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公开(公告)号：US08318445B2
公开(公告)日：2012-11-27
申请号：US12349811
申请日：2009-01-07
IPC分类号： G01N33/553 , G01N33/569
CPC分类号： G01N33/56972 , G01N33/54333 , G01N33/569 , G01N33/58
摘要： The present invention relates to methods and systems for labeling, isolating, detecting, and/or enumerating a statistically significant number of biological cells, or other biological analytes of interest, present in a complex matrix sample. The isolation of a biological target of interest from a sample mixture is done by immunomagnetic separation. Upon introduction of the sample within an imaging chamber, the capture complex (biological target-magnetic capture agent) will be attracted by the magnetic field and will lay on the surface of the chamber in the focal plane of the imaging system.
摘要翻译： 本发明涉及用于标记，分离，检测和/或列举存在于复合基质样品中的统计学上显着数量的生物细胞或感兴趣的其他生物分析物的方法和系统。 从样品混合物中分离感兴趣的生物靶是通过免疫磁性分离进行的。 当在成像室内引入样品时，捕获复合物（生物目标 - 磁捕获剂）将被磁场吸引并且将位于成像系统的焦平面中的室的表面上。
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2.发明授权Methods and systems for altering fluorescent intensities of a plurality of particles 有权用于改变多个颗粒的荧光强度的方法和系统公开(公告)号：US08124943B1
公开(公告)日：2012-02-28
申请号：US11696771
申请日：2007-04-05
CPC分类号： G01N15/1456 , G01N2021/6439
摘要： A method for altering fluorescent emissions of particles includes setting the particles in motion and exposing the moving particles to light such that fluorescent intensities of the particles are lessened isotropically and substantially simultaneously. Another method includes measuring fluorescent emissions of particles, determining the measured fluorescent emissions do not collectively fit within a first predetermined range of fluorescent values, and exposing the particles to one or more incidents of light that are configured to cooperatively alter the fluorescent emissions of the particles to be within a second predetermined range of fluorescent values. An embodiment of an apparatus includes a vessel configured to contain a plurality of particles and a means for setting the particles in motion. The apparatus further includes an illumination subsystem configured to direct light toward the vessel and at a spectral window (i.e., wavelength or band of wavelengths) which is configured to isotropically and substantially simultaneously lessen the fluorescent emissions of each of the particles.
摘要翻译： 用于改变颗粒的荧光发射的方法包括使颗粒运动并使可移动的颗粒暴露于光，使得颗粒的荧光强度各向同性地并且基本上同时减少。 另一种方法包括测量颗粒的荧光发射，确定所测量的荧光发射不共同适合于荧光值的第一预定范围内，并将颗粒暴露于被配置为协调地改变颗粒的荧光发射的一种或多种光的事件 在荧光值的第二预定范围内。 装置的实施例包括构造成容纳多个颗粒的容器和用于设置颗粒运动的装置。 该装置还包括被配置为将光引向容器并且被配置为各向同性地并且基本上同时减少每个颗粒的荧光发射的光谱窗（即波长或波长带）的照明子系统。
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公开(公告)号：US08372590B2
公开(公告)日：2013-02-12
申请号：US12349833
申请日：2009-01-07
IPC分类号： G01N33/543
CPC分类号： G01N33/56966 , G01N33/54326
摘要： The present invention relates to methods and systems for labeling, isolating, detecting and enumerating biological cells, or other biological analytes of interest present in a sample, where the capturing complex may also serve as a labeling agent. In one embodiment, the capture complex is an encoded magnetic bead coupled to antibodies having a specific affinity for a cell surface protein on a cell of interest. The methods and systems of the present invention can be used for quantitative or qualitative detection.
摘要翻译： 本发明涉及用于标记，分离，检测和列举样品中存在的生物细胞或其他生物分析物的方法和系统，其中捕获复合物也可以用作标记试剂。 在一个实施方案中，捕获复合物是与感兴趣的细胞上的细胞表面蛋白具有特异性亲和力的抗体偶联的编码磁珠。 本发明的方法和系统可用于定量或定性检测。
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4.发明授权公开(公告)号：US08296088B2
公开(公告)日：2012-10-23
申请号：US12781550
申请日：2010-05-17
申请人： Wayne D. Roth , Charles J. Collins , William R. Deicher , Jarden E. Krager , Adam R. Schilffarth , Ross G. Johnson , Colin D. Bozarth , Victor Selvaraj , Nicolas F. Arab , Bruce J. C. Bernard , Donald A. Conner , Robert S. Roach
发明人： Wayne D. Roth , Charles J. Collins , William R. Deicher , Jarden E. Krager , Adam R. Schilffarth , Ross G. Johnson , Colin D. Bozarth , Victor Selvaraj , Nicolas F. Arab , Bruce J. C. Bernard , Donald A. Conner , Robert S. Roach
CPC分类号： G01N21/05 , G01N15/1463 , G01N21/274 , G01N21/645 , G01N33/54333 , G01N35/00693 , G01N35/00732 , G01N35/0098 , G01N2015/1452 , G01N2021/0367 , G01N2021/058 , G01N2035/00544 , G01N2035/00564 , G01N2201/1211 , Y10T436/12
摘要： Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.
摘要翻译： 提供了用于执行一种或多种材料的测量的系统和方法。 一个系统被配置为将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积。 另一系统被配置成对测量装置的成像体积中的一种或多种材料进行成像。 附加系统被配置为基本上将一种或多种材料固定在测量装置的成像体积中。 另一系统被配置成将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积，以对成像体积中的一种或多种材料进行成像，以使成像体积中的一种或多种材料基本上固定 ，或其某些组合。
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公开(公告)号：US20090176255A1
公开(公告)日：2009-07-09
申请号：US12349811
申请日：2009-01-07
IPC分类号： G01N33/567 , G01N33/53 , G01N33/569
CPC分类号： G01N33/56972 , G01N33/54333 , G01N33/569 , G01N33/58
摘要： The present invention relates to methods and systems for labeling, isolating, detecting, and/or enumerating a statistically significant number of biological cells, or other biological analytes of interest, present in a complex matrix sample. The isolation of a biological target of interest from a sample mixture is done by immunomagnetic separation. Upon introduction of the sample within an imaging chamber, the capture complex (biological target-magnetic capture agent) will be attracted by the magnetic field and will lay on the surface of the chamber in the focal plane of the imaging system.
摘要翻译： 本发明涉及用于标记，分离，检测和/或列举存在于复合基质样品中的统计学上显着数量的生物细胞或感兴趣的其他生物分析物的方法和系统。 从样品混合物中分离感兴趣的生物靶是通过免疫磁性分离进行的。 当在成像室内引入样品时，捕获复合物（生物目标 - 磁捕获剂）将被磁场吸引并且将位于成像系统的焦平面中的室的表面上。
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公开(公告)号：US20090246796A1
公开(公告)日：2009-10-01
申请号：US12349833
申请日：2009-01-07
IPC分类号： G01N33/53
CPC分类号： G01N33/56966 , G01N33/54326
摘要： The present invention relates to methods and systems for labeling, isolating, detecting and enumerating biological cells, or other biological analytes of interest present in a sample, where the capturing complex may also serve as a labeling agent. In one embodiment, the capture complex is an encoded magnetic bead coupled to antibodies having a specific affinity for a cell surface protein on a cell of interest. The methods and systems of the present invention can be used for quantitative or qualitative detection.
摘要翻译： 本发明涉及用于标记，分离，检测和列举样品中存在的生物细胞或其他生物分析物的方法和系统，其中捕获复合物也可以用作标记试剂。 在一个实施方案中，捕获复合物是与感兴趣的细胞上的细胞表面蛋白具有特异性亲和力的抗体偶联的编码磁珠。 本发明的方法和系统可用于定量或定性检测。
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