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    TRANSCRIPTION FACTORS FOR CELLULOSIC ENZYME PRODUCTION
    1.
    发明申请
    TRANSCRIPTION FACTORS FOR CELLULOSIC ENZYME PRODUCTION 有权
    用于细胞生产的转录因子

    公开(公告)号：US20140220641A1

    公开(公告)日：2014-08-07

    申请号：US14233735

    申请日：2012-07-23

    申请人： Chaoguang Tian ,  Teresa Shock ,  N. Louise Glass ,  Samuel Coradetti ,  James Craig

    发明人： Chaoguang Tian ,  Teresa Shock ,  N. Louise Glass ,  Samuel Coradetti ,  James Craig

    IPC分类号： C12P19/14 ,  C12P19/02 ,  C12N15/80

    CPC分类号： C12P19/14 ,  C07K14/37 ,  C12N15/80 ,  C12P19/02

    摘要： Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases. The disclosure relates inter alia to a method of degrading cellulose-containing material, to a method of increasing the production of one or more cellulases from a fungal cell and to a method of reducing the viscosity of a pretreated biomass material, by contacting pretreated biomass material with a fungal host cell containing at least one recombinant nucleic acid encoding clr-2 or a related transcription factor.

    摘要翻译： 本文提供了用于增加一种或多种纤维素酶从真菌宿主细胞产生的方法和组合物。 本发明基于惊人的发现，即转录调节因子clr-2在丝状真菌细胞中的错误表达能够在非诱导或饥饿条件下诱导纤维素酶基因的表达，导致纤维素酶从细胞中的分泌增加 。 有利的是，在不存在纤维素或纤维二糖的情况下培养的丝状真菌细胞中转录因子clr-2的错误表达导致纤维素酶的分泌增加。 本公开尤其涉及将含纤维素材料降解的方法，一种增加一种或多种纤维素酶从真菌细胞产生的方法，以及降低预处理的生物质材料的粘度的方法，通过使预处理的生物质材料 与含有至少一种编码clr-2或相关转录因子的重组核酸的真菌宿主细胞。

    Transcription factors for cellulosic enzyme production
    2.
    发明授权
    Transcription factors for cellulosic enzyme production 有权
    纤维素酶生产的转录因子

    公开(公告)号：US09441255B2

    公开(公告)日：2016-09-13

    申请号：US14233735

    申请日：2012-07-23

    申请人： Chaoguang Tian ,  Teresa Shock ,  N. Louise Glass ,  Samuel Coradetti ,  James Craig

    发明人： Chaoguang Tian ,  Teresa Shock ,  N. Louise Glass ,  Samuel Coradetti ,  James Craig

    IPC分类号： C12P19/14 ,  C07K14/37 ,  C12N15/80 ,  C12P19/02

    CPC分类号： C12P19/14 ,  C07K14/37 ,  C12N15/80 ,  C12P19/02

    摘要： Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases. The disclosure relates inter alia to a method of degrading cellulose-containing material, to a method of increasing the production of one or more cellulases from a fungal cell and to a method of reducing the viscosity of a pretreated biomass material, by contacting pretreated biomass material with a fungal host cell containing at least one recombinant nucleic acid encoding clr-2 or a related transcription factor.

    摘要翻译： 本文提供了用于增加一种或多种纤维素酶从真菌宿主细胞产生的方法和组合物。 本发明基于惊人的发现，即转录调节因子clr-2在丝状真菌细胞中的错误表达能够在非诱导或饥饿条件下诱导纤维素酶基因的表达，导致纤维素酶从细胞中的分泌增加 。 有利的是，在不存在纤维素或纤维二糖的情况下培养的丝状真菌细胞中转录因子clr-2的错误表达导致纤维素酶的分泌增加。 本公开尤其涉及将含纤维素材料降解的方法，一种增加一种或多种纤维素酶从真菌细胞产生的方法，以及降低预处理的生物质材料的粘度的方法，通过使预处理的生物质材料 与含有至少一种编码clr-2或相关转录因子的重组核酸的真菌宿主细胞。