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1.发明申请METHODS AND COMPOSITIONS FOR IMPROVING SUGAR TRANSPORT, MIXED SUGAR FERMENTATION, AND PRODUCTION OF BIOFUELS 有权改善蔗糖运输，混合糖发酵和生产生物柴油的方法和组合物公开(公告)号：US20110020910A1
公开(公告)日：2011-01-27
申请号：US12843844
申请日：2010-07-26
申请人： N.Louise C. GLASS , Chaoguang Tian , William T. Beeson, IV , Huimin Zhao , Jing Du , Jin Ho Choi , James H. Doudna Cate , Jonathan M. Galazka , Suk Jin Ha , Yong-Su Jin , Soo Rin Kim , Sijin Li , Xiaomin Yang
发明人： N.Louise C. GLASS , Chaoguang Tian , William T. Beeson, IV , Huimin Zhao , Jing Du , Jin Ho Choi , James H. Doudna Cate , Jonathan M. Galazka , Suk Jin Ha , Yong-Su Jin , Soo Rin Kim , Sijin Li , Xiaomin Yang
摘要： The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.
摘要翻译： 本公开内容涉及含有编码多肽的重组多核苷酸的宿主细胞，其中多肽将纤维葡聚糖转运入细胞。 本发明进一步涉及增加纤维素递送到细胞中的方法，增加细胞在含有纤维葡聚糖的培养基上生长的方法，共发酵纤维素衍生和半纤维素衍生的糖的方法，以及制备烃或烃的方法 通过提供含有编码多肽的重组多核苷酸的宿主细胞，其中所述多肽将纤维葡聚糖转运到细胞中。 本公开涉及含有编码多肽的重组多核苷酸的宿主细胞，其中多肽将戊糖转运入细胞，增加戊糖转运至细胞中的方法，在含有戊糖的培养基上增加细胞生长的方法，以及 通过提供含有编码多肽的重组多核苷酸的宿主细胞来制备烃或烃衍生物的方法，其中多肽将戊糖转运到细胞中。
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公开(公告)号：US08431360B2
公开(公告)日：2013-04-30
申请号：US12843844
申请日：2010-07-26
申请人： N. Louise Glass , Chaoguang Tian , William T. Beeson, IV , Huimin Zhao , Jing Du , Jin Ho Choi , James H. Doudna Cate , Jonathan M. Galazka , Suk-Jin Ha , Yong-Su Jin , Soo Rin Kim , Sijin Li , Xiaomin Yang
发明人： N. Louise Glass , Chaoguang Tian , William T. Beeson, IV , Huimin Zhao , Jing Du , Jin Ho Choi , James H. Doudna Cate , Jonathan M. Galazka , Suk-Jin Ha , Yong-Su Jin , Soo Rin Kim , Sijin Li , Xiaomin Yang
摘要： The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.
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3.发明申请公开(公告)号：US20140154751A1
公开(公告)日：2014-06-05
申请号：US13980866
申请日：2012-01-20
申请人： James H. Doudna Cate , William T. Beeson, IV , Jonathan M. Galazka , Huimin Zhao , Sijin Li , Yong-Su Jin , Suk-Jin Ha
发明人： James H. Doudna Cate , William T. Beeson, IV , Jonathan M. Galazka , Huimin Zhao , Sijin Li , Yong-Su Jin , Suk-Jin Ha
CPC分类号： C12P19/12 , C07K14/195 , C07K14/37 , C12N9/2445 , C12N9/90 , C12P7/10 , C12P19/14 , C12P19/16 , C12P19/24 , Y02E50/16 , Y02E50/17
摘要： The present disclosure provides compositions and methods for the fermentation of cellodextrins and β-D-glucose. Host cells and recombinant polypeptides having glucose mutarotase activity are provided. Additionally, methods for improving cell growth, production of chemicals, and consumption of cellodextrins and β-D-glucose during fermentation of mixtures containing cellodextrins and β-D-glucose are provided.
摘要翻译： 本公开提供了用于发酵纤维糊精和β-葡萄糖的组合物和方法。 提供具有葡萄糖变异酶活性的宿主细胞和重组多肽。 另外，提供了用于改善细胞生长，化学品生产和在含有纤维糊精和β-葡萄糖的混合物发酵过程中消耗纤维糊精和消耗D-葡萄糖的方法。
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公开(公告)号：US20130295631A1
公开(公告)日：2013-11-07
申请号：US13877144
申请日：2011-10-03
申请人： Huimin Zhao , Byoungjin Kim , Jing Du , Yongbo Yuan , Dawn Eriksen , Tong Si
发明人： Huimin Zhao , Byoungjin Kim , Jing Du , Yongbo Yuan , Dawn Eriksen , Tong Si
IPC分类号： C12P7/06
CPC分类号： C12P7/06 , C07K14/39 , C12N9/0006 , C12N9/1205 , C12N9/2445 , C12N15/1093 , C12N15/52 , C12N15/81 , C12P7/10 , C12Y101/01009 , C12Y101/0101 , C12Y101/01307 , C12Y207/01017 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to the production of highly efficient heterologous pathways in host cells by identifying favorable enzyme and/or promoter combinations. In particular the present disclosure provides methods for assembly and selection of multi-step xylose and arabinose/xylose utilization pathways from a library of fungal enzymes. The present disclosure further provides compositions containing favorable enzyme combinations, as well as recombinant yeast expressing such combinations, and methods of use for bioconversion of pentose sugars. Also provided are compositions and methods involving favorable expression patterns identified by utilization of combinations of promoters of varying strengths. Provided herein are methods for assembly and selection of multi-step xylose, arabinose/xylose, and cellobiose utilization pathways from a library of promoters of varying strengths. The present disclosure further provides compositions containing heterologous enzyme-coding polynucleotides under the control of favorable promoters, as well as recombinant yeast expressing such enzymes, and methods of their use for bioconversion of pentose and/or hexose sugars.
摘要翻译： 本公开涉及通过鉴定有利的酶和/或启动子组合在宿主细胞中产生高效的异源途径。 特别地，本公开提供了从真菌酶文库中组装和选择多步木糖和阿拉伯糖/木糖利用途径的方法。 本公开进一步提供含有有利的酶组合的组合物，以及表达这种组合的重组酵母以及用于戊糖的生物转化的方法。 还提供了包括通过利用不同强度的启动子的组合鉴定的有利的表达模式的组合物和方法。 本文提供了用于从不同强度的启动子文库中组装和选择多步木糖，阿拉伯糖/木糖和纤维二糖利用途径的方法。 本公开进一步提供了含有有利启动子控制的异源酶编码多核苷酸的组合物，以及表达这些酶的重组酵母，以及它们用于戊糖和/或己糖的生物转化的方法。
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5.发明申请公开(公告)号：US20120052570A1
公开(公告)日：2012-03-01
申请号：US13145752
申请日：2010-01-22
IPC分类号： C12N5/071
CPC分类号： C07K14/721 , C07K2319/715 , C12N9/90
摘要： The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor α, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site-specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.
摘要翻译： 本发明涉及在诱导包含所述目的蛋白质的融合蛋白的活性和含有人的突变配体结合结构域的ERM多肽时紧密地暂时地控制脊椎动物中感兴趣的蛋白质的生物学活性的方法 雌激素受体α与不影响雌激素信号传导的合成配体。 特别地，本发明涉及通过用合成配体诱导包含位点特异性重组酶蛋白和ERM多肽的融合蛋白的活性，在脊椎动物，优选小鼠中产生紧密的时间控制的靶向体细胞突变的方法 没有雌激素和抗雌激素活性。
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公开(公告)号：US07442773B2
公开(公告)日：2008-10-28
申请号：US11040686
申请日：2005-01-21
申请人： Huimin Zhao , Olga Esteban
发明人： Huimin Zhao , Olga Esteban
CPC分类号： C40B30/04
摘要： The present invention provides a universal peptide-binding scaffold. This scaffold is used to bind a target. The target can be a peptide or peptides of interest (for example, peptides associated with a disease state) or can represent the entire proteome. The target can be either protein fragments prepared by enzymatic digestion of the entire proteome or N- or C-terminal short sequences exposed by chemical denaturation of the entire proteome (unfolded proteins). The universal peptide-binding scaffold can be tailored to specifically bind a target using the methods described herein.
摘要翻译： 本发明提供通用的肽结合支架。 此支架用于绑定目标。 该靶可以是感兴趣的肽或肽（例如，与疾病状态相关的肽）或可以代表整个蛋白质组。 靶标可以是通过酶切消化整个蛋白质组或通过整个蛋白质组（未折叠的蛋白质）的化学变性暴露的N-或C-末端短序列制备的蛋白质片段。 可以使用本文所述的方法来调整通用肽结合支架以特异性结合靶标。
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公开(公告)号：US20080241900A1
公开(公告)日：2008-10-02
申请号：US12123279
申请日：2008-05-19
IPC分类号： C12P7/18
CPC分类号： C12P7/06 , C12N9/0006 , C12P7/18 , C12P7/40 , C12P19/02 , C12Y101/01021 , Y02E50/16 , Y02E50/17
摘要： A new xylose reductase encoding gene from Neurspora crassa was heterologously expressed in E. coli as a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.
摘要翻译： 来自粗糙脉孢菌的新的木糖还原酶编码基因在大肠杆菌中作为His标签融合蛋白异源表达，随后以高产率纯化。 显示该木糖还原酶具有高的周转率和催化效率，在室温下的高稳定性，宽的pH曲线以及NADPH对NADH的偏好。 该酶用于生产木糖醇和其它糖醇如山梨糖醇，以及用于将植物生物质发酵成乙醇的生物体的代谢增强。
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8.发明申请公开(公告)号：US20050026250A1
公开(公告)日：2005-02-03
申请号：US10865146
申请日：2004-06-10
CPC分类号： C12N9/0004 , C12P19/36
摘要： Phosphite dehydrogenase mutant enzymes were generated that provide relaxed cofactor specificity and increased thermostability over the wild type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.
摘要翻译： 产生亚磷酸脱氢酶突变酶，其提供比野生型酶更宽松的辅因子特异性和增加的热稳定性。 突变体酶可用于烟酰胺辅因子再生。
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公开(公告)号：US10106824B2
公开(公告)日：2018-10-23
申请号：US13521366
申请日：2011-01-14
申请人： Huimin Zhao , Nikhil Unni Nair , Michael Racine , Ryan Woodyer
发明人： Huimin Zhao , Nikhil Unni Nair , Michael Racine , Ryan Woodyer
IPC分类号： C12P7/18
摘要： Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.
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公开(公告)号：US20100291645A1
公开(公告)日：2010-11-18
申请号：US12667269
申请日：2008-07-10
申请人： Huimin Zhao , Nikhil Unni Nair
发明人： Huimin Zhao , Nikhil Unni Nair
CPC分类号： C12N9/0006 , C12N9/0004 , C12P7/065 , C12P7/18 , Y02E50/17
摘要： Engineered mutant xylose reductases demonstrate higher preference to xylose than arabinose. Amino acid mutations were engineered in to native xylose reductase from Neurospora crassa. Mutant xylose reductases are useful in the production of xylitol and ethanol.
摘要翻译： 工程化的突变木糖还原酶表现出比阿拉伯糖更优先于木糖。 将氨基酸突变工程改造为来自粗糙脉孢菌的天然木糖还原酶。 突变木糖还原酶可用于生产木糖醇和乙醇。
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