公开(公告)号：US09441255B2

公开(公告)日：2016-09-13

申请号：US14233735

申请日：2012-07-23

申请人： Chaoguang Tian ,  Teresa Shock ,  N. Louise Glass ,  Samuel Coradetti ,  James Craig

发明人： Chaoguang Tian ,  Teresa Shock ,  N. Louise Glass ,  Samuel Coradetti ,  James Craig

IPC分类号： C12P19/14 ,  C07K14/37 ,  C12N15/80 ,  C12P19/02

CPC分类号： C12P19/14 ,  C07K14/37 ,  C12N15/80 ,  C12P19/02

摘要： Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases. The disclosure relates inter alia to a method of degrading cellulose-containing material, to a method of increasing the production of one or more cellulases from a fungal cell and to a method of reducing the viscosity of a pretreated biomass material, by contacting pretreated biomass material with a fungal host cell containing at least one recombinant nucleic acid encoding clr-2 or a related transcription factor.

摘要翻译： 本文提供了用于增加一种或多种纤维素酶从真菌宿主细胞产生的方法和组合物。 本发明基于惊人的发现，即转录调节因子clr-2在丝状真菌细胞中的错误表达能够在非诱导或饥饿条件下诱导纤维素酶基因的表达，导致纤维素酶从细胞中的分泌增加 。 有利的是，在不存在纤维素或纤维二糖的情况下培养的丝状真菌细胞中转录因子clr-2的错误表达导致纤维素酶的分泌增加。 本公开尤其涉及将含纤维素材料降解的方法，一种增加一种或多种纤维素酶从真菌细胞产生的方法，以及降低预处理的生物质材料的粘度的方法，通过使预处理的生物质材料 与含有至少一种编码clr-2或相关转录因子的重组核酸的真菌宿主细胞。

公开(公告)号：US20140220641A1

公开(公告)日：2014-08-07

申请号：US14233735

申请日：2012-07-23

申请人： Chaoguang Tian ,  Teresa Shock ,  N. Louise Glass ,  Samuel Coradetti ,  James Craig

发明人： Chaoguang Tian ,  Teresa Shock ,  N. Louise Glass ,  Samuel Coradetti ,  James Craig

IPC分类号： C12P19/14 ,  C12P19/02 ,  C12N15/80

CPC分类号： C12P19/14 ,  C07K14/37 ,  C12N15/80 ,  C12P19/02

摘要： Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases. The disclosure relates inter alia to a method of degrading cellulose-containing material, to a method of increasing the production of one or more cellulases from a fungal cell and to a method of reducing the viscosity of a pretreated biomass material, by contacting pretreated biomass material with a fungal host cell containing at least one recombinant nucleic acid encoding clr-2 or a related transcription factor.

摘要翻译： 本文提供了用于增加一种或多种纤维素酶从真菌宿主细胞产生的方法和组合物。 本发明基于惊人的发现，即转录调节因子clr-2在丝状真菌细胞中的错误表达能够在非诱导或饥饿条件下诱导纤维素酶基因的表达，导致纤维素酶从细胞中的分泌增加 。 有利的是，在不存在纤维素或纤维二糖的情况下培养的丝状真菌细胞中转录因子clr-2的错误表达导致纤维素酶的分泌增加。 本公开尤其涉及将含纤维素材料降解的方法，一种增加一种或多种纤维素酶从真菌细胞产生的方法，以及降低预处理的生物质材料的粘度的方法，通过使预处理的生物质材料 与含有至少一种编码clr-2或相关转录因子的重组核酸的真菌宿主细胞。

公开(公告)号：US10121387B1

公开(公告)日：2018-11-06

申请号：US15867962

申请日：2018-01-11

申请人： James Craig

发明人： James Craig

IPC分类号： G09B15/08 ,  G09B15/00 ,  G09B1/00 ,  G09B15/02

摘要： A system of teaching piano playing and music theory having piano cards. Piano cards are a flash card system with piano note information on each card. These piano cards are placed above piano keys of a piano or other keyboard designating keys to be played in a specific scale. Black and white triangles on these piano cards designate ebony and ivory keys to be played and can only be placed on a keyboard correctly if aligning white and black triangles with ebony and ivory keys. Triangles designate fingers to be used on fingering cards by way of numbers designating fingers. On music theory cards numbers inside triangles designate scale degrees. On chord cards numbers designate degrees to be played. This music teaching system further comprises a music wheel that has been color coded designating the key a scale is in.

公开(公告)号：US5178181A

公开(公告)日：1993-01-12

申请号：US786706

申请日：1991-11-01

申请人： James Craig

发明人： James Craig

IPC分类号： B01F1/00 ,  B01F5/04

CPC分类号： B01F5/0496 ,  B01F1/0027 ,  Y10T137/4891

摘要： A fertilizer applicator is provided for use with a sprinkler line system. The applicator comprises a fertilizer container with integrally formed internal inlet and outlet tubes. Water is fed through an inlet-outlet tube into the bottom of the fertilizer container, where it dissolves or dilutes some of the solid or liquid fertilizer and forms a leached, concentrated solution. This concentrate continuously rises through the fertilizer and into the outlet tube where it drains downwardly. The concentrate is then diluted with water coming through the inlet-outlet tube including a venturi, the size of which controls the flow rate of the concentrate. The diluted concentrate is then passed into the sprinkler system for application to vegetation. The applicator has a single piece, simplified design, without moving parts, and does not leak fertilizer into the drain. Also, the bore arrangement of the applicator enables the device to be drained and then filled with fertilizer, without fertilizer leakage, followed by running water through the fertilizer at any selected time, and this renders the operation more automatic.

摘要翻译： 提供了一种用于洒水管线系统的肥料施加器。 涂抹器包括具有整体形成的内部入口和出口管的肥料容器。 水通过入口 - 出口管进入肥料容器的底部，其中它溶解或稀释一些固体或液体肥料并形成浸出的浓缩溶液。 这种浓缩物不断地通过肥料上升到排出管下方的出口管中。 然后将浓缩物用通过入口 - 出口管的水稀释，包括文氏管，其大小控制浓缩物的流速。 然后将稀释的浓缩物进入喷洒系统以施用于植被。 涂抹器具有单件，简化设计，无移动部件，不会将肥料泄漏到排水管中。 此外，涂布器的孔布置使得装置能够排出，然后用肥料填充，没有肥料泄漏，随后在任何选定的时间将水通过肥料，这使得操作更自动化。

公开(公告)号：US20100202816A1

公开(公告)日：2010-08-12

申请号：US12762137

申请日：2010-04-16

申请人： David Block ,  James Craig

发明人： David Block ,  James Craig

IPC分类号： B41J25/34 ,  B41J11/66

CPC分类号： B41J15/042 ,  B41J3/4075 ,  B41J3/54

摘要： A printing device comprising a casing having a first part housing a first printing mechanism which comprises a printhead and a platen located at a print zone, and a holder for holding a roll of precut label stock in a position to pass through the print zone, and a second part housing a second printing mechanism which comprises a printhead and a platen located at a print zone and means for receiving a cassette housing image receiving tape in a position to pass through the print zone.

摘要翻译： 一种打印装置，包括壳体，该壳体具有容纳包括打印头和位于打印区域的压板的第一打印机构的第一部分，以及用于保持预切割标签纸卷在穿过打印区域的位置的保持器，以及 第二部分，其容纳第二打印机构，该第二打印机构包括位于打印区域的打印头和台板，以及用于接收在通过打印区域的位置中的盒子容纳图像接收带的盒的装置。

公开(公告)号：US20090233032A1

公开(公告)日：2009-09-17

申请号：US12075347

申请日：2008-03-11

申请人： James Craig

发明人： James Craig

IPC分类号： B32B33/00

CPC分类号： A61F9/025 ,  G02C7/00 ,  Y10T428/1471

摘要： One embodiment of the lens protectors attached to pre-manufactured safety lenses, or goggles. The lens protectors are made up of clear self adhesive plastic strips, with easy to use peeling tabs. Each set of lens protectors contains several peel-off protective strips.

摘要翻译： 附接到预制安全镜片或护目镜的镜片保护器的一个实施例。 透镜保护膜由透明的自粘塑料条组成，易于使用的剥离片。 每组镜头保护膜包含几个剥离保护条。

公开(公告)号：US07275881B2

公开(公告)日：2007-10-02

申请号：US10964920

申请日：2004-10-15

申请人： David Block ,  James Craig ,  Patrick R. Reitz

发明人： David Block ,  James Craig ,  Patrick R. Reitz

IPC分类号： B41J1/50

CPC分类号： B41J3/54 ,  B41J3/4075

摘要： A printing system comprising: a printing device having first and second similar printing mechanisms; first and second similar holding means for holding respective first and second rolls of label stock in a position to pass the first and second printing mechanisms respectively; and a local processor connected to supply print data to a selected one of said first and second printing mechanisms and comprising means for receiving print data and a command for supplying said print data to the selected one of said first and second printing mechanisms to print an image on a label of the respective associated one of said first and second rolls of label stock, based on said command; and a host computer connected to the printing system and arranged to execute a common printer driver for supplying said print data and command to the printing device, regardless of which printing mechanism is selected.

摘要翻译： 一种打印系统，包括：具有第一和第二类似打印机构的打印装置; 第一和第二类似的保持装置，用于将各自的第一和第二标签纸卷保持在分别通过第一和第二印刷机构的位置; 以及本地处理器，其连接以向所述第一和第二打印机构中的所选择的一个提供打印数据，并且包括用于接收打印数据的装置和用于将所述打印数据提供给所述第一和第二打印机构中的所选打印机的打印图像的命令 基于所述命令，在所述第一和第二标签纸卷上分别相关联的一个标签上; 以及连接到打印系统的主计算机，并且被布置成执行用于将打印数据和命令提供给打印装置的公共打印机驱动程序，而不管选择了哪个打印机构。

公开(公告)号：US07241920B2

公开(公告)日：2007-07-10

申请号：US11420742

申请日：2006-05-27

申请人： Erik J. Mori ,  Brian Hong ,  James Craig

发明人： Erik J. Mori ,  Brian Hong ,  James Craig

IPC分类号： C09K13/00

CPC分类号： C09K13/08

摘要： An improved aqueous soluble surfactant which has particular utility for incorporating in etchants for semiconductor devices is provided. The surfactant comprises a combination of a linear perfluorocarboxylic acid, a cyclic amine and an aliphatic alcohol.

摘要翻译： 提供了一种改进的水溶性表面活性剂，其特别适用于掺入用于半导体器件的蚀刻剂中。 表面活性剂包括直链全氟羧酸，环胺和脂族醇的组合。

公开(公告)号：US20080044215A1

公开(公告)日：2008-02-21

申请号：US11844626

申请日：2007-08-24

申请人： David Block ,  James Craig ,  Patrick Reitz

发明人： David Block ,  James Craig ,  Patrick Reitz

IPC分类号： B41J1/50

CPC分类号： B41J3/54 ,  B41J3/4075

摘要： A printing system comprising: a printing device having first and second similar printing mechanisms; first and second similar holding means for holding respective first and second rolls of label stock in a position to pass the first and second printing mechanisms respectively; and a local processor connected to supply print data to a selected one of said first and second printing mechanisms and comprising means for receiving print data and a command for supplying said print data to the selected one of said first and second printing mechanisms to print an image on a label of the respective associated one of said first and second rolls of label stock, based on said command; and a host computer connected to the printing system and arranged to execute a common printer driver for supplying said print data and command to the printing device, regardless of which printing mechanism is selected.

摘要翻译： 一种打印系统，包括：具有第一和第二类似打印机构的打印装置; 第一和第二类似的保持装置，用于将各自的第一和第二标签纸卷保持在分别通过第一和第二印刷机构的位置; 以及本地处理器，其连接以向所述第一和第二打印机构中的所选择的一个提供打印数据，并且包括用于接收打印数据的装置和用于将所述打印数据提供给所述第一和第二打印机构中的所选打印机的打印图像的命令 基于所述命令，在所述第一和第二标签纸卷上分别相关联的一个标签上; 以及连接到打印系统的主计算机，并且被布置成执行用于将打印数据和命令提供给打印装置的公共打印机驱动程序，而不管选择了哪个打印机构。

公开(公告)号：US20070078751A1

公开(公告)日：2007-04-05

申请号：US11242448

申请日：2005-10-03

申请人： James Craig

发明人： James Craig

IPC分类号： G06Q40/00

CPC分类号： G06Q20/023 ,  G06Q20/02 ,  G06Q20/12 ,  G06Q20/40 ,  G06Q40/04

摘要： A system for conducting commerce across a distributed network includes a remote consumer computer. A merchant server communicates with the remote consumer computer across an open network to enable purchasing of a product from the remote consumer computer. A financial clearing house server is located within a secured closed network and communicates with the remote consumer computer and the merchant server across the secured closed network. The merchant server creates a merchant ID, receipt ID and transaction total corresponding to a consumer transaction and sends the merchant ID, receipt ID, and transaction ID to the financial clearing house server and the remote consumer computer. The financial clearing house server arranges payment of the transaction in response to a matching merchant ID, receipt ID, and transaction total from the remote consumer computer.

摘要翻译： 用于在分布式网络上进行商务的系统包括远程消费者计算机。 商家服务器通过开放网络与远程消费者计算机进行通信，以便能够从远程消费者计算机购买产品。 金融清算所服务器位于安全的封闭网络内，并通过安全的封闭网络与远程消费者计算机和商家服务器进行通信。 商家服务器创建与消费者交易相对应的商家ID，收据ID和交易总额，并将商家ID，收据ID和交易ID发送到金融结算所服务器和远程消费者计算机。 金融结算所服务器响应于来自远程消费者计算机的匹配商家ID，收据ID和交易总额来安排交易的支付。