公开(公告)号：US06989235B2

公开(公告)日：2006-01-24

申请号：US10365378

申请日：2003-02-11

申请人： Lars Chapsky ,  Wayne D. Frasch ,  Chia Fu Chou ,  Frederic Zenhausern ,  Herbert Goronkin

发明人： Lars Chapsky ,  Wayne D. Frasch ,  Chia Fu Chou ,  Frederic Zenhausern ,  Herbert Goronkin

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： B82Y5/00 ,  C12Q1/6825 ,  C12Q2563/155

摘要： An exemplary system and method of employing DNA hybridization for the detection of bio-agents is disclosed as comprising inter alia a biomolecular rotary motor (150); a capture probe DNA fragment (140) effectively attached to said biomolecular motor (150); a target DNA fragment (130) suitably adapted for hybridization with said capture probe DNA (140); a signal probe DNA fragment (120) suitably adapted for hybridization with said target DNA (130); and a fluorescent bead (100) attached to said signal probe DNA (120). Disclosed features and specifications may be variously controlled, adapted or otherwise optionally modified to improve certain device fabrication parameters and/or performance metrics.

摘要翻译： 公开了使用DNA杂交来检测生物试剂的示例性系统和方法，其特别包括生物分子旋转马达（150）; 有效连接到所述生物分子马达（150）的捕获探针DNA片段（140）; 适于与所述捕获探针DNA（140）杂交的靶DNA片段（130）; 适于与所述靶DNA（130）杂交的信号探针DNA片段（120）; 和连接到所述信号探针DNA（120）上的荧光珠（100）。 公开的特征和规范可以被不同地控制，适应或以其他方式任意地修改以改进某些设备制造参数和/或性能度量。

公开(公告)号：US06824664B1

公开(公告)日：2004-11-30

申请号：US09707892

申请日：2000-11-06

申请人： Robert H. Austin ,  Jonas O. Tegenfeldt ,  Edward C. Cox ,  Chia Fu Chou ,  Olgica Bakajin

发明人： Robert H. Austin ,  Jonas O. Tegenfeldt ,  Edward C. Cox ,  Chia Fu Chou ,  Olgica Bakajin

IPC分类号： C02F140

CPC分类号： B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2400/0421 ,  B01L2400/0424 ,  B03C5/024 ,  B03C5/026

摘要： The present invention further provides a device for the integrated micromanipulation, amplification, and analysis of polarized particles such as DNA comprises a microchip which contains constrictions of insulating material for dielectrophoresis powered by an alternating current or direct current signal generator, and attached to a hot source that can be heated to specific temperatures. Nucleic acids can be heated and cooled to allow for denaturation, and the annealing of complementary primers and enzymatic reactions, as in a thermocycling reaction. After such a reaction has been completed at the constriction, the dielectrophoretic field can be switched to a direct field to release the product and direct it through a matrix for fractionation. The device includes data analysis equipment for the control of these operations, and imaging equipment for the analysis of the products. The invention permits the efficient handling of minute samples in large numbers, since reactions occur while sample material is trapped between constrictions. Because the positioning, reactions, and release into a fractioning matrix all occur at the constriction which serves as a focusing locus, the need to transfer samples into different tubes is eliminated, thus increasing the efficiency and decreasing the possibility of damage to the samples.

摘要翻译： 本发明还提供了用于对诸如DNA的极化粒子进行集成显微操作，放大和分析的装置，包括微芯片，其包含用于由交流电或直流信号发生器供电的介电电泳的绝缘材料的收缩部分，并连接到热源 可以加热到特定温度。 核酸可被加热和冷却以允许变性，以及互补引物的退火和酶反应，如在热循环反应中。 在收缩处完成这样的反应之后，介电电泳场可以切换到直接场以释放产物并将其引导通过基质进行分馏。 该设备包括用于控制这些操作的数据分析设备和用于分析产品的成像设备。 本发明允许有效地处理微量样品，因为在样品材料被捕获在收缩之间时发生反应。 因为定位，反应和释放到分级矩阵中都会发生在用作聚焦轨迹的收缩处，消除了将样品转移到不同管中的需要，从而提高了效率并降低了样品损伤的可能性。

公开(公告)号：US06858436B2

公开(公告)日：2005-02-22

申请号：US10135790

申请日：2002-04-30

申请人： Frederic Zenhausern ,  Chia-Fu Chou

发明人： Frederic Zenhausern ,  Chia-Fu Chou

IPC分类号： G01N21/55 ,  G01N30/60 ,  G01N30/74 ,  G01N30/86 ,  G01Q30/12 ,  G01Q60/18 ,  G01N21/00

CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N30/44 ,  G01N30/6095 ,  G01N30/74 ,  G01N30/78 ,  G01N30/8617 ,  G01N30/8631

摘要： An exemplary system and method for detecting at least one analyte in a sample comprises inter alia a source of radiation (300), a near-field aperture array (315), a chromatographic field (330), a detector (350), and a data processor (370). Disclosed features and specifications may be variously controlled, adapted or otherwise optionally modified to improve detection of any sub-diffraction-limited scale phenomena. Exemplary embodiments of the present invention representatively provide for improved S/N, increased sample throughput, refined spectral resolution and enhanced detection sensitivity.

摘要翻译： 用于检测样品中至少一种分析物的示例性系统和方法尤其包括辐射源（300），近场孔径阵列（315），色谱场（330），检测器（350）和 数据处理器（370）。 公开的特征和规范可以被不同地控制，适应或以其他方式任意地修改，以改进任何次衍射受限尺度现象的检测。 本发明的示例性实施例代表性地提供了改进的S / N，增加的采样通量，精确的光谱分辨率和增强的检测灵敏度。

公开(公告)号：US06203683B1

公开(公告)日：2001-03-20

申请号：US09188284

申请日：1998-11-09

申请人： Robert H. Austin ,  Edward C. Cox ,  Chia-Fu Chou

发明人： Robert H. Austin ,  Edward C. Cox ,  Chia-Fu Chou

IPC分类号： G01N2726

CPC分类号： B01L7/52 ,  G01N27/44743

摘要： A device for the integrated micromanipulation, amplification, and analysis of polyelectrolytes such as DNA comprises a microchip which contains electrodes for dielectrophoresis powered by an AC signal generator, and a trapping electrode attached to a direct current source that can be heated to specific temperatures. Nucleic acids can be heated and cooled to allow for denaturation, the annealing of complementary primers and enzymatic reactions, as in a thermocycling reaction. After such a reaction has been completed on the trapping electrode, the dielectrophoretic field can be switched to a direct current to release the product and direct it through a matrix for fractionation and/or analysis. The device includes data analysis equipment for the control of these operations, and imaging equipment for the analysis of the products. The invention permits the efficient handling of minute samples in large numbers, since reactions occur while sample material is positioned on an electrode in a microfluidic channel. Because the positioning, reactions, and release into a fractioning matrix are all integrated from the focusing wire, the need to transfer samples into different tubes is eliminated, thus increasing the efficiency and decreasing the possibility of damage to the samples.

摘要翻译： 用于聚电解质如DNA的集成显微操作，扩增和分析的装置包括微芯片，其包含用于由AC信号发生器供电的介电电泳的电极和附接到可被加热到特定温度的直流电源的捕获电极。 核酸可被加热和冷却以允许变性，互补引物的退火和酶反应，如在热循环反应中。 在捕获电极上完成这样的反应之后，介电电泳场可以转换成直流电流以释放产物并将其引导通过基质进行分级和/或分析。 该设备包括用于控制这些操作的数据分析设备和用于分析产品的成像设备。 本发明允许大量有效处理微小样品，因为当样品材料位于微流体通道中的电极上时发生反应。 因为定位，反应和释放到分级矩阵中都是从聚焦线集成的，因此消除了将样品转移到不同管中的需要，从而提高了效率并降低了样品损伤的可能性。

公开(公告)号：US06986601B2

公开(公告)日：2006-01-17

申请号：US10437515

申请日：2003-05-13

申请人： Daniel J. Sadler ,  Rajnish G. Changrani ,  Chia Fu Chou ,  Frederic Zenhausern

发明人： Daniel J. Sadler ,  Rajnish G. Changrani ,  Chia Fu Chou ,  Frederic Zenhausern

IPC分类号： B01F11/02

CPC分类号： B01F11/0062 ,  B01F13/0059 ,  B01F2215/0098 ,  B01F2215/0454 ,  B01L3/5027

摘要： A method for mixing at least two fluids includes introducing the at least two fluids into a mixing chamber. The mixing chamber includes a piezoelectric component (500) for mechanical actuation of fluid motion within or adjacent the mixing chamber. The piezoelectric component includes at least first (400), second (410), third (420), and fourth (430) actuation domains, the first and third actuation domains being on first and third opposed sides of the piezoelectric component, and the second and fourth actuation domains being on second and fourth opposed sides of the piezoelectric component. The first and third domains are actuated at a first phase of a frequency of oscillation, and the second and fourth domains are actuated at a second phase of the frequency of oscillation.

摘要翻译： 用于混合至少两种流体的方法包括将至少两种流体引入混合室。 混合室包括用于在混合室内或邻近混合室内的流体运动的机械致动的压电元件（500）。 压电元件至少包括第一（400），第二（410），第三（420）和第四（430）致动域，第一和第三致动域位于压电元件的第一和第三相对侧上，第二 并且第四致动域位于压电元件的第二和第四相对侧上。 第一和第三域在振荡频率的第一阶段被激励，并且第二和第四域在振荡频率的第二阶段被致动。