Method for identification and quantification of short or small RNA molecules
    2.
    发明申请
    Method for identification and quantification of short or small RNA molecules 审中-公开
    用于鉴定和定量短或小RNA分子的方法

    公开(公告)号:US20060063181A1

    公开(公告)日:2006-03-23

    申请号:US11204903

    申请日:2005-08-15

    IPC分类号: C12Q1/68 C12P19/34

    摘要: A method of identifying and quantifying small RNA molecules comprising a) isolating RNA molecules; b) ligating RNA adapter molecules onto the isolated RNA molecules to form RNA template molecules; c) forming complementary DNA molecules by transcribing the RNA template molecules; d) amplifying the complementary DNA molecules; e) obtaining sequence information of the complementary DNA molecules (and thereby the RNA from which it was derived); and f) obtaining quantity information of the complementary DNA molecules, wherein the quantity information of the DNA molecules reflects the quantity of the isolated RNA molecules is provided. Included in the invention is the identification of RNA molecules between 15 and 30 nucleotides in length.

    摘要翻译: 鉴定和定量小RNA分子的方法,包括:a)分离RNA分子; b)将RNA衔接子分子连接到分离的RNA分子上以形成RNA模板分子; c)通过转录RNA模板分子形成互补DNA分子; d)扩增互补DNA分子; e)获得互补DNA分子的序列信息(从而获得衍生自其的RNA); 以及f)获得所述互补DNA分子的量信息,其中所述DNA分子的量信息反映分离的RNA分子的量。 本发明中包括鉴定长度在15至30个核苷酸之间的RNA分子。