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公开(公告)号：US08062876B2
公开(公告)日：2011-11-22
申请号：US12097027
申请日：2006-12-21
申请人： Robert W. Mallett , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
发明人： Robert W. Mallett , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
IPC分类号： C12N9/74
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.
摘要翻译： 公开了将凝血酶原-1转化为凝血酶的方法。 将凝血酶原-1的水溶液施加到固定在固体支持物上的西葫芦C上，从而提供每毫升固体支持物500mg至4000mg的凝血酶原-1和凝血酶原-1与西葫芦之间的接触时间 -C为1.8至3.5分钟。 所得到的活性凝血酶可以在离子交换色谱介质或亲和层析介质上捕获。
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公开(公告)号：US06437102B1
公开(公告)日：2002-08-20
申请号：US09638275
申请日：2000-08-14
IPC分类号： A23J100
CPC分类号： C07K1/30 , C07K14/47 , Y10S435/962 , Y10S435/975
摘要： Disclosed is a method for separating prions from biological materials. The method includes adding a polyalkylene glycol, such as polyethylene glycol, to a solution of the biological material such that a precipitate containing the prion is formed. This precipitate is then separated from the solution of biological material, thereby removing prions. Biological materials include biologically derived fluids, such as cerebrospinal fluid, biological samples, such as brain homogenates, blood plasma fractions, and aqueous solutions of recombinantly produced products. The disclosed method provides an effective process for the removal of these infectious materials from the biological materials, which may be further processed to provide the therapeutic compositions.
摘要翻译： 公开了从生物材料中分离朊病毒的方法。 该方法包括将聚亚烷基二醇例如聚乙二醇加入到生物材料的溶液中，使得形成含有朊病毒的沉淀物。 然后将该沉淀物与生物材料的溶液分离，从而除去朊病毒。 生物材料包括生物衍生的流体，如脑脊液，生物样品，如脑匀浆，血浆级分和重组产物的水溶液。 所公开的方法提供了从生物材料中去除这些感染性物质的有效方法，其可进一步加工以提供治疗组合物。
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3.发明授权公开(公告)号：US08759066B2
公开(公告)日：2014-06-24
申请号：US12935235
申请日：2009-04-07
CPC分类号： C12N9/6418 , C07K2319/50
摘要： Disclosed are compositions for activating thrombin precursors to thrombin. The compositions provided include polypeptide compositions wherein the pre-pro-sequence comprises a thrombin cleavage site. The compositions provided also include polynucleotides encoding said polypeptides and recombinant systems for expressing said polypeptides. This disclosure also relates to methods for producing said compositions, recovering said compositions, activating said compositions purifying said compositions and producing active thrombin molecules using the active form of said compositions.
摘要翻译： 公开了用于激活凝血酶凝血酶前体的组合物。 提供的组合物包括多肽组合物，其中前序列包含凝血酶切割位点。 提供的组合物还包括编码所述多肽的多核苷酸和用于表达所述多肽的重组系统。 本公开还涉及生产所述组合物的方法，回收所述组合物，使用所述组合物的活性形式活化所述组合物来纯化所述组合物并产生活性凝血酶分子。
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4.发明申请公开(公告)号：US20110151536A1
公开(公告)日：2011-06-23
申请号：US12935235
申请日：2009-04-07
CPC分类号： C12N9/6418 , C07K2319/50
摘要： Disclosed are compositions for activating thrombin precursors to thrombin. The compositions provided include polypeptide compositions wherein the pre-pro-sequence comprises a thrombin cleavage site. The compositions provided also include polynucleotides encoding said polypeptides and recombinant systems for expressing said polypeptides. This disclosure also relates to methods for producing said compositions, recovering said compositions, activating said compositions purifying said compositions and producing active thrombin molecules using the active form of said compositions.
摘要翻译： 公开了用于激活凝血酶凝血酶前体的组合物。 提供的组合物包括多肽组合物，其中前序列包含凝血酶切割位点。 提供的组合物还包括编码所述多肽的多核苷酸和用于表达所述多肽的重组系统。 本公开还涉及生产所述组合物的方法，回收所述组合物，使用所述组合物的活性形式活化所述组合物来纯化所述组合物并产生活性凝血酶分子。
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5.发明授权Process for the production of a reversibly inactive acidified plasmin composition 无效用于生产可逆失活的酸化纤溶酶组合物的方法公开(公告)号：US07544500B2
公开(公告)日：2009-06-09
申请号：US10692105
申请日：2003-10-23
申请人： Rita T. Bradley , Scott A. Cook , Christopher A. Dadd , Jonathan D. Kent , Marina N. Korneyeva , Valery V. Novokhatny , James F. Rebbeor , Christopher J. Stenland , Jonathan S. Strauss , Jarrett C. Terry , Jeffrey A. Yuziuk
发明人： Rita T. Bradley , Scott A. Cook , Christopher A. Dadd , Jonathan D. Kent , Marina N. Korneyeva , Valery V. Novokhatny , James F. Rebbeor , Christopher J. Stenland , Jonathan S. Strauss , Jarrett C. Terry , Jeffrey A. Yuziuk
CPC分类号： A61K38/4833 , A61K38/484 , A61K47/02 , A61K47/12 , A61K47/183 , A61K47/26 , C12N9/6435 , C12Y304/21007
摘要： Disclosed is both a process for producing a reversibly inactive acidified plasmin by activating plasminogen and a process for producing a purified plasminogen. The produced plasmin is isolated and stored with a low pH-buffering capacity agent to provide a substantially stable formulation. The purified plasminogen is typically purified from a fraction obtained in the separation of immunoglobulin from Fraction II+III chromatographic process and eluted at a low pH. The reversibly inactive acidified plasmin may be used in the administration of a thrombolytic therapy.
摘要翻译： 本发明公开了通过活化纤溶酶原产生可逆失活的酸化纤溶酶的方法和用于产生纯化的纤溶酶原的方法。 分离产生的纤溶酶并用低pH缓冲能力试剂储存以提供基本上稳定的制剂。 纯化的纤溶酶原通常从从级分II + III色谱法分离免疫球蛋白获得的级分中纯化，并在低pH下洗脱。 可逆惰性的酸化纤溶酶可以用于溶栓治疗的施用。
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公开(公告)号：US20080293122A1
公开(公告)日：2008-11-27
申请号：US12097027
申请日：2006-12-21
申请人： Robert W. Mallet , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
发明人： Robert W. Mallet , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
IPC分类号： C12N9/74
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.
摘要翻译： 公开了将凝血酶原-1转化为凝血酶的方法。 将凝血酶原-1的水溶液施加到固定在固体支持物上的西葫芦C上，从而提供每毫升固体支持物500mg至4000mg的凝血酶原-1和凝血酶原-1与西葫芦之间的接触时间 -C为1.8至3.5分钟。 所得到的活性凝血酶可以在离子交换色谱介质或亲和层析介质上捕获。
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公开(公告)号：US06913695B2
公开(公告)日：2005-07-05
申请号：US10614904
申请日：2003-07-08
申请人： Nathan C. Jones , Marina N. Korneyeva , James F. Rebbeor , Richard Scott Rosenthal , Christopher J. Stenland
发明人： Nathan C. Jones , Marina N. Korneyeva , James F. Rebbeor , Richard Scott Rosenthal , Christopher J. Stenland
摘要： A method of sanitizing chromatographic media is provided. The method includes contacting the media with an acidic chaotropic agent, at low temperature and low pH. The method provides pathogen removal and/or inactivation, including viral inactivation in particular embodiments.
摘要翻译： 提供了一种消毒色谱介质的方法。 该方法包括在低温和低pH下使介质与酸性离液剂接触。 该方法提供病原体去除和/或灭活，包括特定实施方案中的病毒灭活。
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