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公开(公告)号：US20080293122A1
公开(公告)日：2008-11-27
申请号：US12097027
申请日：2006-12-21
申请人： Robert W. Mallet , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
发明人： Robert W. Mallet , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
IPC分类号： C12N9/74
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.
摘要翻译： 公开了将凝血酶原-1转化为凝血酶的方法。 将凝血酶原-1的水溶液施加到固定在固体支持物上的西葫芦C上，从而提供每毫升固体支持物500mg至4000mg的凝血酶原-1和凝血酶原-1与西葫芦之间的接触时间 -C为1.8至3.5分钟。 所得到的活性凝血酶可以在离子交换色谱介质或亲和层析介质上捕获。
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公开(公告)号：US5986049A
公开(公告)日：1999-11-16
申请号：US457254
申请日：1995-06-01
IPC分类号： C12N15/09 , A61K38/00 , A61P7/06 , C07K1/18 , C07K1/22 , C07K1/26 , C07K7/06 , C07K14/52 , C07K14/715 , C12P21/02 , C12R1/91 , C07K1/00
CPC分类号： C07K14/715 , C07K14/52 , A61K38/00
摘要： Purified mammalian thrombopoietin proteins and methods of making them are disclosed. The proteins are characterized by a M.sub.r =70,000.+-.10,000 daltons as determined by SDS-polyacrylamide gel electrophoresis under denaturing conditions and are at least 90% pure with respect to contaminating proteins. The proteins can be prepared by a method in which thrombopoietin is adsorbed to and eluted from a polypeptide comprising a ligand-binding domain of an MPL receptor, then the eluted thrombopoietin is fractionated by anion exchange chromatography.
摘要翻译： 公开了纯化的哺乳动物血小板生成蛋白及其制备方法。 蛋白质的特征在于Mr = 70,000 +/- 10,000道尔顿，在变性条件下通过SDS-聚丙烯酰胺凝胶电泳测定，并且相对于污染蛋白质至少为90％纯度。 蛋白质可以通过血小板生成素被吸附到包含MPL受体的配体结合结构域的多肽中并从其中洗脱的方法来制备，然后通过阴离子交换色谱分离洗脱的血小板生成素。
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3.发明授权
公开(公告)号：US5827734A
公开(公告)日：1998-10-27
申请号：US540242
申请日：1995-10-04
IPC分类号： A61K38/00 , C07K14/575 , C12N5/00 , C07K16/00
CPC分类号： C07K14/5759 , A61K38/00
摘要： Methods for determining ob protein in a biological sample are disclosed. In a preferred embodiment, the biological samples are analyzed using an ELISA capable of detecting and quantitating ob protein.
摘要翻译： 公开了用于测定生物样品中ob蛋白的方法。 在优选的实施方案中，使用能够检测和定量ob蛋白的ELISA分析生物样品。
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公开(公告)号：US08062876B2
公开(公告)日：2011-11-22
申请号：US12097027
申请日：2006-12-21
申请人： Robert W. Mallett , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
发明人： Robert W. Mallett , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
IPC分类号： C12N9/74
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.
摘要翻译： 公开了将凝血酶原-1转化为凝血酶的方法。 将凝血酶原-1的水溶液施加到固定在固体支持物上的西葫芦C上，从而提供每毫升固体支持物500mg至4000mg的凝血酶原-1和凝血酶原-1与西葫芦之间的接触时间 -C为1.8至3.5分钟。 所得到的活性凝血酶可以在离子交换色谱介质或亲和层析介质上捕获。
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公开(公告)号：US5858967A
公开(公告)日：1999-01-12
申请号：US938001
申请日：1997-09-19
IPC分类号： C07K14/575 , A01N37/18 , C07K14/74 , C12P21/06
CPC分类号： C07K14/5759
摘要： Methods for identifying factors that regulate appetite are disclosed. Preferably, the methods feature determinination of decreased food consumption in selected recipient mammals following administration of a test sample. A product having appetite suppressing activity is also described. In addition, methods for reducing food consumption in a mammal, featuring administration of a pharmaceutical composition comprising a representative appetite suppression factor, are disclosed.
摘要翻译： 公开了确定食欲调节因素的方法。 优选地，所述方法的特征在于在施用测试样品之后确定所选择的受体哺乳动物中食物消耗减少。 还描述了具有食欲抑制活性的产品。 此外，公开了减少哺乳动物食物消耗的方法，其特征在于包括代表性食欲抑制因子的药物组合物的给药。
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公开(公告)号：US5744587A
公开(公告)日：1998-04-28
申请号：US484246
申请日：1995-06-07
申请人： Andrew R. Alaska , Jin-Jyi Chang , William Downey , John W. Forstrom , Linh Phan
发明人： Andrew R. Alaska , Jin-Jyi Chang , William Downey , John W. Forstrom , Linh Phan
CPC分类号： C07K14/524
摘要： Methods of removing protein contaminants from a solution comprising thrombopoietin are disclosed. The solution is exposed to hydroxyapatite, whereby protein contaminants are bound to the hydroxyapatite and the thrombopoietin remains substantially unbound, and the unbound thrombopoietin is recovered. The use of hydroxyapatite is advantageously combined with other purification and concentration techniques, including ion-exchange chromatography, ligand affinity chromatography, hydrophobic interaction chromatography, ultrafiltration, and differential precipitation.
摘要翻译： 公开了从包含血小板生成素的溶液中除去蛋白质污染物的方法。 溶液暴露于羟基磷灰石，由此蛋白质污染物与羟基磷灰石结合，血小板生成素基本上未结合，并且回收未结合的血小板生成素。 羟磷灰石的使用有利地与其它纯化和浓缩技术组合，包括离子交换层析，配体亲和层析，疏水相互作用层析，超滤和差示沉淀。
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公开(公告)号：US5241049A
公开(公告)日：1993-08-31
申请号：US455698
申请日：1989-12-22
IPC分类号： A61K38/00 , A61P29/00 , C07K1/20 , C07K5/08 , C07K5/10 , C07K7/06 , C07K7/08 , C07K14/00 , C07K14/47 , C07K14/52 , C12N15/09 , C12P21/02 , C12R1/91
摘要： Substantially pure porcine proteins having chemoattractant activity for neutrophils and methods for preparing the proteins are provided. These proteins are useful within compositions for enhancing neutrophil chemotaxis, such as in promoting the formation of granulation tissue. The proteins may also be used within methods for developing inflammatory and anti-inflammatory agents.
摘要翻译： 提供了对嗜中性粒细胞具有化学吸收活性的基本纯化的猪蛋白质以及制备蛋白质的方法。 这些蛋白质在用于增强嗜中性粒细胞趋化性的组合物中是有用的，例如促进肉芽组织的形成。 蛋白质也可用于发展炎症和抗炎剂的方法中。
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