公开(公告)号：US08383766B2

公开(公告)日：2013-02-26

申请号：US11893341

申请日：2007-08-15

Applicant: Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

Inventor： Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

IPC: C07K14/00

CPC classification number: C07K14/00 ,  C07K1/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K2299/00 ,  C12N15/102 ,  C12N15/62 ,  C12Q1/6886 ,  C12Q2600/156 ,  G01N33/5308 ,  G01N33/57484 ,  G01N33/6875 ,  G01N2500/04

Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.

Abstract translation: 锌指结合到三联体或四核苷酸靶位点的特异性取决于锌指在多角蛋白中的位置，并且因此取决于其目标亚位点在较大靶序列内的位置。 本公开提供了锌指氨基酸序列，用于识别具有子部位的5'-最多位置的核苷酸G的相对于靶位点内的亚位点的位置优化的三重态目标亚位点。 因此，本公开提供了用于识别GNN靶亚属的手指位置特异性氨基酸序列。 这允许构建具有改善其靶序列的亲和性和特异性的多指锌指蛋白，以及增强的生物活性。

公开(公告)号：US20090246841A1

公开(公告)日：2009-10-01

申请号：US12079274

申请日：2008-03-26

Applicant: Andrew C. Jamieson ,  Mark Anthony Vandeyar

Inventor： Andrew C. Jamieson ,  Mark Anthony Vandeyar

IPC: C12P7/06 ,  C12P7/24

CPC classification number: C12P7/40 ,  C12N1/12 ,  C12N1/16 ,  C12N1/20 ,  C12N9/88 ,  C12P7/06 ,  C12P7/24 ,  C12Y401/01001 ,  Y02E50/17

Abstract: The present disclosure provides methods for producing acetaldehyde from renewable biological resources, for example, from a fermentable substrate, with the advantages of energy efficiency and ease of purification. Particular embodiments, feature the production of acetaldehyde from pyruvate, wherein the pyruvate is generated from various carbon sources (e.g., sugars) by pyruvate-producing microorganisms. The methods comprise culturing a pyruvate-producing microorganism in a culture medium under conditions such that pyruvate is excreted and accumulates extracellularly, resulting in a pyruvate-enriched medium.

Abstract translation: 本公开提供了从可再生生物资源例如可发酵底物生产乙醛的方法，具有能量效率和易于纯化的优点。 具体实施方案的特征在于从丙酮酸生产乙醛，其中丙酮酸盐由产生丙酮酸的微生物的各种碳源（例如糖）产生。 所述方法包括在培养基中培养产生丙酮酸的微生物，使得丙酮酸盐被排泄并在细胞外积聚，产生富含丙酮酸的培养基。

公开(公告)号：US07585849B2

公开(公告)日：2009-09-08

申请号：US11202009

申请日：2005-08-11

Applicant: Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

Inventor： Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

IPC: A01N43/04 ,  A01N65/00 ,  A61K31/70 ,  A61K38/00 ,  C12N15/00 ,  C07K5/00 ,  C07K7/00 ,  C07K16/00 ,  C07K17/00 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C07K14/00 ,  C07K1/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K2299/00 ,  C12N15/102 ,  C12N15/62 ,  C12Q1/6886 ,  C12Q2600/156 ,  G01N33/5308 ,  G01N33/57484 ,  G01N33/6875 ,  G01N2500/04

Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.

Abstract translation: 锌指结合到三联体或四核苷酸靶位点的特异性取决于锌指在多角蛋白中的位置，并且因此取决于其目标亚位点在较大靶序列内的位置。 本公开提供了锌指氨基酸序列，用于识别具有子部位的5'-最多位置的核苷酸G的相对于靶位点内的亚位点的位置优化的三重态目标亚位点。 因此，本公开提供了用于识别GNN靶亚属的手指位置特异性氨基酸序列。 这允许构建具有改善其靶序列的亲和性和特异性的多指锌指蛋白，以及增强的生物活性。

公开(公告)号：US20080113406A1

公开(公告)日：2008-05-15

申请号：US11748432

申请日：2007-05-14

Applicant: David W. Martin ,  Andrew C. Jamieson ,  Dean M. Scholl ,  Steven R. Williams

Inventor： David W. Martin ,  Andrew C. Jamieson ,  Dean M. Scholl ,  Steven R. Williams

IPC: C07K14/195 ,  C12N1/21 ,  C07H21/04 ,  C12P21/06 ,  C12N15/74

CPC classification number: C07K14/21 ,  C07K2319/35 ,  C07K2319/74

Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.

Abstract translation: 公开了天然存在的细菌素的修饰形式，例如铜绿假单胞菌的R型py虫。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或适应因子）的方法。

公开(公告)号：US20160289708A1

公开(公告)日：2016-10-06

申请号：US15089364

申请日：2016-04-01

Applicant: Andrew C. JAMIESON ,  Mark Anthony VANDEYAR

Inventor： Andrew C. JAMIESON ,  Mark Anthony VANDEYAR

IPC: C12P7/40 ,  C12N9/88

CPC classification number: C12P7/40 ,  C12N1/12 ,  C12N1/16 ,  C12N1/20 ,  C12N9/88 ,  C12P7/06 ,  C12P7/24 ,  C12Y401/01001 ,  Y02E50/17

Abstract: The present disclosure provides methods for producing acetaldehyde from renewable biological resources, for example, from a fermentable substrate, with the advantages of energy efficiency and ease of purification. Particular embodiments, feature the production of acetaldehyde from pyruvate, wherein the pyruvate is generated from various carbon sources (e.g., sugars) by pyruvate-producing microorganisms. The methods comprise culturing a pyruvate-producing microorganism in a culture medium under conditions such that pyruvate is excreted and accumulates extracellularly, resulting in a pyruvate-enriched medium.

Abstract translation: 本公开提供了从可再生生物资源例如可发酵底物生产乙醛的方法，具有能量效率和易于纯化的优点。 具体实施方案的特征在于从丙酮酸生产乙醛，其中丙酮酸盐由产生丙酮酸的微生物的各种碳源（例如糖）产生。 所述方法包括在培养基中培养产生丙酮酸的微生物，使得丙酮酸盐被排泄并在细胞外积聚，产生富含丙酮酸的培养基。

公开(公告)号：US07732586B2

公开(公告)日：2010-06-08

申请号：US11748432

申请日：2007-05-14

Applicant: David W. Martin, Jr. ,  Andrew C. Jamieson ,  Dean M. Scholl ,  Steven R. Williams

Inventor： David W. Martin, Jr. ,  Andrew C. Jamieson ,  Dean M. Scholl ,  Steven R. Williams

IPC: C07H21/04 ,  C12P21/04

CPC classification number: C07K14/21 ,  C07K2319/35 ,  C07K2319/74

Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.

Abstract translation: 公开了天然存在的细菌素的修饰形式，例如铜绿假单胞菌的R型py虫。 细菌素在其尾纤维的末端被修饰，负责对其同源结合配偶体或受体（例如细菌表面上的那些）的结合特异性和亲和力。 还描述了在细菌表面上使用修饰的细菌素，例如结合受体（包括毒力或适应因子）的方法。

公开(公告)号：US20080242847A1

公开(公告)日：2008-10-02

申请号：US11893341

申请日：2007-08-15

Applicant: Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

Inventor： Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

IPC: C07K14/00

CPC classification number: C07K14/00 ,  C07K1/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K2299/00 ,  C12N15/102 ,  C12N15/62 ,  C12Q1/6886 ,  C12Q2600/156 ,  G01N33/5308 ,  G01N33/57484 ,  G01N33/6875 ,  G01N2500/04

Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.

Abstract translation: 锌指结合到三联体或四核苷酸靶位点的特异性取决于锌指在多角蛋白中的位置，并且因此取决于其目标亚位点在较大靶序列内的位置。 本公开提供了锌指氨基酸序列，用于识别具有子部位的5'-最多位置的核苷酸G的相对于靶位点内的亚位点的位置优化的三重态目标亚位点。 因此，本公开提供了用于识别GNN靶亚属的手指位置特异性氨基酸序列。 这允许构建具有改善其靶序列的亲和性和特异性的多指锌指蛋白，以及增强的生物活性。

公开(公告)号：US07030215B2

公开(公告)日：2006-04-18

申请号：US09990186

申请日：2001-11-20

Applicant: Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

Inventor： Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

IPC: A61K38/00 ,  A23J1/00 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C07K1/00 ,  C07K2299/00 ,  G01N33/5308 ,  G01N33/6875 ,  G01N2500/04

Abstract: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.