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公开(公告)号:US09315860B2
公开(公告)日:2016-04-19
申请号:US13504394
申请日:2010-10-26
申请人: Claus Becker , Dmitry Cherkasov
发明人: Claus Becker , Dmitry Cherkasov
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6853 , C12Q1/6806 , C12Q1/686 , C12Q2563/179
摘要: The invention relates to a novel method for enzymatically marking nucleic acid chains (target sequences) by using nucleotide conjugates. Said nucleotide conjugates are capable of binding specifically to the target sequence under reaction conditions and of being incorporated in the complementary growing strand by means of a polymerase. The nucleic acid chains marked with such conjugates can be bound to the solid phase. The marking can be carried out in parallel with the enzymatic amplification of target sequences.
摘要翻译: 本发明涉及通过使用核苷酸缀合物对核酸链(靶序列)进行酶标记的新方法。 所述核苷酸缀合物能够在反应条件下与靶序列特异性结合,并通过聚合酶并入互补生长链中。 标记有这种缀合物的核酸链可以结合固相。 标记可以与靶序列的酶促扩增并行进行。
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公开(公告)号:US09035035B2
公开(公告)日:2015-05-19
申请号:US11886518
申请日:2006-03-17
申请人: Dmitry Cherkasov , Englbert Bäuml
发明人: Dmitry Cherkasov , Englbert Bäuml
CPC分类号: C07H21/00 , C07H19/06 , C07H19/16 , C12Q1/6869 , C12Q2537/143 , C12Q2525/179
摘要: The invention describes new structures of the nucleotide conjugates (nuc-macromolecules) comprising at lease one nucleotide moiety coupled to at least one macromolecular compound via a short linker. These conjugates can be used as substrates for various kinds of polymerizing enzymes in the enzymatic synthesis of nucleic acids. In particular, these compounds can be used for labeling nucleic acids.
摘要翻译: 本发明描述了包含通过短接头与至少一种大分子化合物偶联的至少一个核苷酸部分的核苷酸缀合物(核 - 大分子)的新结构。 这些缀合物可以用作核酸的酶合成中各种聚合酶的底物。 特别地,这些化合物可用于标记核酸。
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公开(公告)号:US20140295438A1
公开(公告)日:2014-10-02
申请号:US14114506
申请日:2012-04-26
IPC分类号: C12Q1/68
CPC分类号: C12Q1/686 , C12Q1/6846 , C12Q2525/101 , C12Q2563/149 , C12Q2563/179
摘要: The invention relates to a novel method for the enzymatic marking of nucleic acid chains (target sequences) by means of nucleotide conjugates. Said nucleotide conjugates are able, under reaction conditions, to specifically bind to the target sequence and integrate into the complementary growing strand by means of a polymerase. The nucleic acid chains marked by such conjugates can be bound to the solid phase. The marking can be carried out parallel to the enzymatic amplification of target sequences.
摘要翻译: 本发明涉及通过核苷酸缀合物酶标记核酸链(靶序列)的新方法。 所述核苷酸缀合物在反应条件下能够通过聚合酶特异性结合靶序列并整合到互补生长链中。 由这种缀合物标记的核酸链可以结合固相。 标记可以与靶序列的酶促扩增平行进行。
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公开(公告)号:US20130171631A1
公开(公告)日:2013-07-04
申请号:US13504394
申请日:2010-10-26
申请人: Claus Becker , Dmitry Cherkasov
发明人: Claus Becker , Dmitry Cherkasov
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6853 , C12Q1/6806 , C12Q1/686 , C12Q2563/179
摘要: The invention relates to a novel method for enzymatically marking nucleic acid chains (target sequences) by using nucleotide conjugates. Said nucleotide conjugates are capable of binding specifically to the target sequence under reaction conditions and of being incorporated in the complementary growing strand by means of a polymerase. The nucleic acid chains marked with such conjugates can be bound to the solid phase. The marking can be carried out in parallel with the enzymatic amplification of target sequences.
摘要翻译: 本发明涉及通过使用核苷酸缀合物对核酸链(靶序列)进行酶标记的新方法。 所述核苷酸缀合物能够在反应条件下与靶序列特异性结合,并通过聚合酶并入互补生长链中。 标记有这种缀合物的核酸链可以结合固相。 标记可以与靶序列的酶促扩增并行进行。
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公开(公告)号:US20100304368A1
公开(公告)日:2010-12-02
申请号:US12442184
申请日:2007-09-20
摘要: Novel methods for enzymatic synthesis of nucleic acid chains and the substrates for the same are disclosed. The methods are based on a step-wise enzymatic reaction. The sequencing of nucleic acids is an example of the use of the claimed methods.
摘要翻译: 公开了用于酶合成核酸链的新方法及其用途。 该方法基于逐步的酶反应。 核酸的测序是使用所要求保护的方法的实例。
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公开(公告)号:US20100093992A1
公开(公告)日:2010-04-15
申请号:US11886518
申请日:2006-03-17
申请人: Dmitry Cherkasov , Englbert Bäuml
发明人: Dmitry Cherkasov , Englbert Bäuml
IPC分类号: C07H19/00
CPC分类号: C07H21/00 , C07H19/06 , C07H19/16 , C12Q1/6869 , C12Q2537/143 , C12Q2525/179
摘要: The invention relates to the structure, production and use of modified nucleotide and nucleoside building blocks, (nuc-macromolecules).
摘要翻译: 本发明涉及修饰的核苷酸和核苷结构单元(nuc-大分子)的结构,生产和应用。
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公开(公告)号:US08637650B2
公开(公告)日:2014-01-28
申请号:US10578313
申请日:2004-11-05
申请人: Dmitry Cherkasov , Christian Hennig
发明人: Dmitry Cherkasov , Christian Hennig
CPC分类号: C07H21/00 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C12Q1/6848 , C12Q1/6869 , C12Q2525/113 , C12Q2525/197
摘要: The invention relates to novel classes of nucleotides that can be used as substrates for enzymes, e.g. for labeling nucleic acids.
摘要翻译: 本发明涉及可用作酶底物的新型核苷酸,例如, 用于标记核酸。
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公开(公告)号:US20100029494A1
公开(公告)日:2010-02-04
申请号:US10578313
申请日:2004-11-05
申请人: Dmitry Cherkasov , Christian Hennig
发明人: Dmitry Cherkasov , Christian Hennig
IPC分类号: C40B30/04 , C07H21/00 , C07H21/02 , C07H21/04 , C07K14/00 , C12P19/34 , C40B50/14 , C12Q1/68 , C08F283/00 , C07K16/00 , C12N9/96 , C07K9/00
CPC分类号: C07H21/00 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C12Q1/6848 , C12Q1/6869 , C12Q2525/113 , C12Q2525/197
摘要: The invention relates to novel classes of nucleotides that can be used as substrates for enzymes, e.g. for labeling nucleic acids.
摘要翻译: 本发明涉及可用作酶底物的新型核苷酸,例如, 用于标记核酸。
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公开(公告)号:US09994894B2
公开(公告)日:2018-06-12
申请号:US14114506
申请日:2012-04-26
CPC分类号: C12Q1/686 , C12Q1/6846 , C12Q2525/101 , C12Q2563/149 , C12Q2563/179
摘要: The invention relates to a novel method for the enzymatic marking of nucleic acid chains (target sequences) by means of nucleotide conjugates. Said nucleotide conjugates are able, under reaction conditions, to specifically bind to the target sequence and integrate into the complementary growing strand by means of a polymerase. The nucleic acid chains marked by such conjugates can be bound to the solid phase. The marking can be carried out parallel to the enzymatic amplification of target sequences.
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