Conjugates of nucleotides and method for the application thereof
    1.
    发明授权
    Conjugates of nucleotides and method for the application thereof 有权
    核苷酸缀合物及其应用方法

    公开(公告)号:US09315860B2

    公开(公告)日:2016-04-19

    申请号:US13504394

    申请日:2010-10-26

    IPC分类号: C12Q1/68

    摘要: The invention relates to a novel method for enzymatically marking nucleic acid chains (target sequences) by using nucleotide conjugates. Said nucleotide conjugates are capable of binding specifically to the target sequence under reaction conditions and of being incorporated in the complementary growing strand by means of a polymerase. The nucleic acid chains marked with such conjugates can be bound to the solid phase. The marking can be carried out in parallel with the enzymatic amplification of target sequences.

    摘要翻译: 本发明涉及通过使用核苷酸缀合物对核酸链(靶序列)进行酶标记的新方法。 所述核苷酸缀合物能够在反应条件下与靶序列特异性结合,并通过聚合酶并入互补生长链中。 标记有这种缀合物的核酸链可以结合固相。 标记可以与靶序列的酶促扩增并行进行。

    CONJUGATES OF NUCLEOTIDES AND METHOD FOR THE APPLICATION THEREOF
    4.
    发明申请
    CONJUGATES OF NUCLEOTIDES AND METHOD FOR THE APPLICATION THEREOF 有权
    核子结合体及其应用方法

    公开(公告)号:US20130171631A1

    公开(公告)日:2013-07-04

    申请号:US13504394

    申请日:2010-10-26

    IPC分类号: C12Q1/68

    摘要: The invention relates to a novel method for enzymatically marking nucleic acid chains (target sequences) by using nucleotide conjugates. Said nucleotide conjugates are capable of binding specifically to the target sequence under reaction conditions and of being incorporated in the complementary growing strand by means of a polymerase. The nucleic acid chains marked with such conjugates can be bound to the solid phase. The marking can be carried out in parallel with the enzymatic amplification of target sequences.

    摘要翻译: 本发明涉及通过使用核苷酸缀合物对核酸链(靶序列)进行酶标记的新方法。 所述核苷酸缀合物能够在反应条件下与靶序列特异性结合,并通过聚合酶并入互补生长链中。 标记有这种缀合物的核酸链可以结合固相。 标记可以与靶序列的酶促扩增并行进行。