Method for Identifying and Quantifying Organic and Biochemical Substances
    5.
    发明申请
    Method for Identifying and Quantifying Organic and Biochemical Substances 审中-公开
    识别和定量有机和生化物质的方法

    公开(公告)号:US20100184062A1

    公开(公告)日:2010-07-22

    申请号:US12667583

    申请日:2008-07-04

    IPC分类号: C12Q1/68 G01N33/53

    摘要: The invention relates to a method for identifying organic or biochemical substances and for determining their concentration in a fluid medium using a nanogap sensor that comprises at least two electrodes. The invention is characterized in that: a nanogap sensor) with electrodes of different materials is used, a respective probe molecule is bonded to each surface of the two electrodes of the sensor and the free remainder of the probe molecules have at least one bondable group with specificity for bonding to a sought substance or to an analyte molecule in the fluid medium. The analyte molecule has at least two binding sites and passes selectively out of the fluid medium in which it is contained, binds to the free ends of the probe molecules, forming a bridge with the probe molecule, and modifies the resulting impedance between the electrodes. The concentration of the substance in the fluid medium can be determined as a result of the modification.

    摘要翻译: 本发明涉及用于鉴别有机或生物化学物质并使用包含至少两个电极的纳米隙传感器来测定其在流体介质中的浓度的方法。 本发明的特征在于:使用具有不同材料的电极的纳米隙传感器),将相应的探针分子结合到传感器的两个电极的每个表面,并且探针分子的游离剩余部分具有至少一个可结合的基团, 与所寻求的物质或流体介质中的分析物分子结合的特异性。 分析物分子具有至少两个结合位点,并且选择性地从其所含的流体介质中流出,与探针分子的自由端结合,与探针分子形成桥,并修改电极之间产生的阻抗。 流体介质中物质的浓度可以作为修改的结果来确定。