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    Human T-cell line infected with HIV-2 which secretes functionally intact HIV-2 GP160.
    2.
    发明授权
    Human T-cell line infected with HIV-2 which secretes functionally intact HIV-2 GP160. 失效
    感染HIV-2的人类T细胞系分泌功能完整的HIV-2 GP160。

    公开(公告)号：US5766844A

    公开(公告)日：1998-06-16

    申请号：US676865

    申请日：1996-07-03

    申请人： Mangalasseril G. Sarngadharan ,  Vaniambadi S. Kalyanaraman ,  Irene LaRue-Kalisz

    发明人： Mangalasseril G. Sarngadharan ,  Vaniambadi S. Kalyanaraman ,  Irene LaRue-Kalisz

    IPC分类号： G01N33/569 ,  A61K39/00 ,  A61K39/21 ,  A61P31/12 ,  A61P31/18 ,  C07K14/155 ,  C07K14/16 ,  C07K16/10 ,  C12N5/06 ,  C12N7/00 ,  C12P21/02 ,  C12R1/92 ,  C12Q1/70 ,  C07K1/00

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2740/16122

    摘要： A novel cell line chronically infected with HIV-2.sub.NIHZ virus secretes the precursor of the viral envelope proteins in extracellular medium. The cells grow well in serum-free medium which enables the concentration of the conditioned medium of the cells to be greatly increased (>100 fold). Such a concentration step is otherwise impossible to achieve if the cells are grown under conventional conditions using 10% fetal calf serum. A specific affinity procedure is provided to purify the gp160 protein from the concentrated conditioned medium of cultures of 100 liter batches using a mouse monoclonal antibody to the HIV-2 gp41 protein, which is a part of the gp160 protein. This purified HIV-2 gp160 reacts with high specificity and sensitivity with the anti viral antibodies of HIV-2 infected humans by several criteria such as i) ELISA, ii) western blot analysis, and iii) radioimmuno-precipitation of metabolically labeled gp160 from the same cell line. The purified HIV-2 gp160 from this cell line is therefore a diagnostic tool for the detection of HIV-2 infection in humans. In view of the predicted oligomeric property of gp160 and its close conformational similarity to the virion envelope, the purified gp160 can also be useful for the development of vaccine strategies against HIV-2 in humans.

    摘要翻译： 长期感染HIV-2NIHZ病毒的​​新型细胞系分泌细胞外介质中病毒包膜蛋白的前体。 细胞在无血清培养基中生长良好，使得细胞条件培养基的浓度大大增加（> 100倍）。 如果细胞在常规条件下使用10％胎牛血清生长，则这样的浓缩步骤是不可能实现的。 提供特异性亲和性程序以使用作为gp160蛋白的一部分的HIV-2 gp41蛋白的小鼠单克隆抗体从100升批次的培养物的浓缩条件培养基中纯化gp160蛋白。 这种纯化的HIV-2 gp160通过几个标准如HIV-2感染的人的抗病毒抗体具有高特异性和敏感性，例如i）ELISA，ii）蛋白质印迹分析，和iii）代谢标记的gp160的放射免疫沉淀 相同的细胞系。 因此，来自该细胞系的纯化的HIV-2 gp160是用于检测人类中HIV-2感染的诊断工具。 鉴于gp160的预测寡聚特性及其与病毒粒子包膜的紧密构象相似性，纯化的gp160也可用于开发人类针对HIV-2的疫苗策略。

    Human T-cell line infected with HIV-2 which secretes functionally intact HIV-2 GP160
    8.
    发明授权
    Human T-cell line infected with HIV-2 which secretes functionally intact HIV-2 GP160 失效
    感染HIV-2的人类T细胞系分泌功能完整的HIV-2 GP160

    公开(公告)号：US5876732A

    公开(公告)日：1999-03-02

    申请号：US59804

    申请日：1998-04-14

    申请人： Mangalasseril G. Sarngadharan ,  Vaniambadi S. Kalyanaraman ,  Irene LaRue-Kalisz

    发明人： Mangalasseril G. Sarngadharan ,  Vaniambadi S. Kalyanaraman ,  Irene LaRue-Kalisz

    IPC分类号： G01N33/569 ,  A61K39/00 ,  A61K39/21 ,  A61P31/12 ,  A61P31/18 ,  C07K14/155 ,  C07K14/16 ,  C07K16/10 ,  C12N5/06 ,  C12N7/00 ,  C12P21/02 ,  C12R1/92 ,  G01N33/53

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2740/16122

    摘要： A novel cell line chronically infected with HIV-2.sub.NIHZ virus secretes the precursor of the viral envelope proteins in extracellular medium. The cells grow well in serum-free medium which enables the concentration of the conditioned medium of the cells to be greatly increased (>100 fold). Such a concentration step is otherwise impossible to achieve if the cells are grown under conventional conditions using 10% fetal calf serum. A specific affinity procedure is provided to purify the gp160 protein from the concentrated conditioned medium of cultures of 100 liter batches using a mouse monoclonal antibody to the HIV-2 gp41 protein, which is a part of the gp160 protein. This purified HIV-2 gp160 reacts with high specificity and sensitivity with the anti viral antibodies of HIV-2 infected humans by several criteria such as i) ELISA, ii) western blot analysis, and iii) radioimmuno-precipitation of metabolically labeled gp160 from the same cell line. The purified HIV-2 gp160 from this cell line is therefore a diagnostic tool for the detection of HIV-2 infection in humans. In view of the predicted oligomeric property of gp160 and its close conformational similarity to the virion envelope, the purified gp160 can also be useful for the development of vaccine strategies against HIV-2 in humans.

    摘要翻译： 长期感染HIV-2NIHZ病毒的​​新型细胞系分泌细胞外介质中病毒包膜蛋白的前体。 细胞在无血清培养基中生长良好，使得细胞条件培养基的浓度大大增加（> 100倍）。 如果细胞在常规条件下使用10％胎牛血清生长，则这样的浓缩步骤是不可能实现的。 提供特异性亲和性程序以使用作为gp160蛋白的一部分的HIV-2 gp41蛋白的小鼠单克隆抗体从100升批次的培养物的浓缩条件培养基中纯化gp160蛋白。 这种纯化的HIV-2 gp160通过几个标准如HIV-2感染的人的抗病毒抗体具有高特异性和敏感性，例如i）ELISA，ii）蛋白质印迹分析，和iii）代谢标记的gp160的放射免疫沉淀 相同的细胞系。 因此，来自该细胞系的纯化的HIV-2 gp160是用于检测人类中HIV-2感染的诊断工具。 鉴于gp160的预测寡聚特性及其与病毒粒子包膜的紧密构象相似性，纯化的gp160也可用于开发人类针对HIV-2的疫苗策略。