中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

96 records (took 0.025 seconds)

    Method for propagating adenoviral vectors encoding inhibitory gene products
    7.
    发明授权
    Method for propagating adenoviral vectors encoding inhibitory gene products 有权
    繁殖编码抑制性基因产物的腺病毒载体的方法

    公开(公告)号：US09388429B2

    公开(公告)日：2016-07-12

    申请号：US14288493

    申请日：2014-05-28

    Applicant: GenVec, Inc.

    Inventor： Jason G. D. Gall ,  Douglas E. Brough ,  C. Richter King

    IPC: C12N7/01 ,  C12N15/861 ,  C12N15/86 ,  C12N7/00 ,  C12N15/63

    CPC classification number: C12N15/635 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/006

    Abstract: The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (b) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.

    Abstract translation: 本发明提供了一种繁殖腺病毒载体的方法。 该方法包括（a）提供包含细胞基因组的细胞，所述细胞基因组包含编码四环素操纵子阻遏蛋白（tetR）的核酸序列，和（b）使细胞与包含编码毒性蛋白质的异源核酸序列的腺病毒载体接触。 异源核酸序列可操作地连接到启动子和一个或多个四环素操纵子操纵子序列（tetO），并且异源核酸序列的表达在tetR存在下被抑制，使得腺病毒载体被繁殖。 本发明还提供了包含上述细胞和腺病毒载体的系统。

    E1-revertant-free adenoviral composition
    8.
    发明申请
    E1-revertant-free adenoviral composition 审中-公开
    E1无反应蛋白腺病毒组合物

    公开(公告)号：US20040209247A1

    公开(公告)日：2004-10-21

    申请号：US10844133

    申请日：2004-05-12

    Applicant: GenVec, Inc.

    Inventor： Douglas E. Brough ,  Imre Kovesdi

    IPC: C12Q001/70

    CPC classification number: C12N15/86 ,  A61K48/00 ,  C12N2710/10343

    Abstract: The invention provides a composition comprising particles of an adenoviral vector comprising deficiencies in two or more gene functions required for viral replication, wherein at least one of the deficiencies is of a gene function of the E1 region of the adenoviral genome and (b) a carrier therefor, with relatively high ratios of (i) the number of particles of the adenoviral vectors to the number of particles of E1-revertant replication-deficient adenoviral vectors not comprising one or more of the deficiencies in gene functions of the E1 region of the adenoviral and (ii) the number of particles of the adenoviral vectors to the number of particles of replication-competent adenoviral vectors, as well as a method of preparing such a composition.

    Abstract translation: 本发明提供包含腺病毒载体颗粒的组合物，其包含病毒复制所需的两个或更多个基因功能的缺陷，其中至少一个缺陷是腺病毒基因组的E1区域的基因功能，和（b）载体 因此，具有相对高的比例（i）腺病毒载体的颗粒数与E1复发缺失型复制缺陷型腺病毒载体的颗粒数量不包含腺病毒E1区的基因功能的一个或多个缺陷 和（ii）腺病毒载体的颗粒数与复制能力的腺病毒载体的颗粒数目，以及制备这种组合物的方法。

    Adenoviral vector and related system and methods of making and use
    9.
    发明申请
    Adenoviral vector and related system and methods of making and use 审中-公开
    腺病毒载体及相关系统及制作及使用方法

    公开(公告)号：US20040161848A1

    公开(公告)日：2004-08-19

    申请号：US10778832

    申请日：2004-02-13

    Applicant: GenVec, Inc.

    Inventor： Imre Kovesdi

    IPC: A61K048/00 ,  C12N015/861

    CPC classification number: C12N7/00 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/002 ,  C12N2830/85

    Abstract: An adenoviral vector comprising an adenoviral genome comprising (i) at least one deletion in a region of the adenoviral genome selected from the group consisting of E1, E2A and E4, (ii) (a) at least one deletion in the VAI gene of the adenoviral genome, alone or in further combination with at least one deletion in the VAII gene of the adenoviral genome, (b) a recombinant VAI gene, alone or in further combination with a recombinant VAII gene, wherein the recombinant gene comprises either of a regulatable promoter in place of the native promoter or a mutated native promoter and 5null to the mutated native promoter, a pol II promoter, or (c) a dominant negative, double-stranded, RNA-dependent protein kinase (PKR) and, optionally, (iii) a polymerase II (pol II) construct comprising a pol II promoter operably linked to a coding region and/or a polymerase III (pol III) construct comprising a pol III promoter operably linked to a coding region, as well as a system comprising such an adenoviral vector and a cell line that complements the adenoviral vector, and related systems and methods.

    Abstract translation: 一种腺病毒载体，其包含腺病毒基因组，其包含（i）在选自E1，E2A和E4的腺病毒基因组的区域中的至少一个缺失，（ii）（a）在所述腺病毒基因的VAI基因中的至少一个缺失 腺病毒基因组单独或与腺病毒基因组的VAII基因中的至少一个缺失进一步组合，（b）单独的或与重组VAII基因进一步组合的重组VAI基因，其中重组基因包括可调节的 启动子代替天然启动子或突变的天然启动子和5'至突变的天然启动子，pol II启动子，或（c）显性失活的，双链的RNA依赖性蛋白激酶（PKR） （iii）包含可操作地连接到编码区的pol II启动子和/或包含可操作地连接到编码区的pol III启动子的聚合酶III（pol III）构建体的聚合酶II（pol II）构建体，以及系统 包括这样的腺病毒 ector和补充腺病毒载体的细胞系，以及相关系统和方法。

    Non-adenoviral gene product-based complementing cells for adenoviral vectors
    10.
    发明申请
    Non-adenoviral gene product-based complementing cells for adenoviral vectors 失效
    用于腺病毒载体的非腺病毒基因产物互补细胞

    公开(公告)号：US20040063203A1

    公开(公告)日：2004-04-01

    申请号：US10695605

    申请日：2003-10-28

    Applicant: GenVec, Inc.

    Inventor： Douglas E. Brough ,  Jason G. D. Gall ,  Imre Kovesdi

    IPC: C12N005/08 ,  C12N015/861

    CPC classification number: C12N7/00 ,  C12N2710/10051 ,  C12N2710/10052 ,  C12N2710/10351 ,  C12N2710/10352

    Abstract: The invention provides cells and methods of using the cells for the propagation of replication-deficient adenoviral vectors. The cells comprise at least one heterologous nucleic acid sequence which upon expression produces at least one non-adenoviral gene product that complements in trans for a deficiency in at least one essential gene function of one or more regions of an adenoviral genome so as to propagate a replication-deficient adenoviral vector comprising an adenoviral genome deficient in the at least one essential gene function of the one or more regions when present in the cell.

    Abstract translation: 本发明提供了细胞和使用细胞促进复制缺陷型腺病毒载体的方法。 所述细胞包含至少一种异源核酸序列，其在表达时产生至少一种非腺病毒基因产物，所述非腺病毒基因产物在腺病毒基因组的一个或多个区域的至少一个必需基因功能缺陷中互补互补，从而传播 复制缺陷型腺病毒载体，其包含存在于细胞中时所述一个或多个区域的至少一个必需基因功能缺陷的腺病毒基因组。

Patent Agency Ranking