公开(公告)号：US06538178B1

公开(公告)日：2003-03-25

申请号：US08120703

申请日：1993-09-13

Applicant: Ganesh Murthy Kishore

Inventor： Ganesh Murthy Kishore

IPC: C12N1582

CPC classification number: C12N9/1241 ,  C12N15/8235 ,  C12N15/8245 ,  C12N15/8247

Abstract: Transformed plant cells which have increased'starch content are disclosed. Also disclosed are whole plants comprising plant cells which express CTP/ADPglucose pyrophosphorylase genes.

Abstract translation: 公开了具有增加的淀粉含量的转化的植物细胞。 还公开了包含表达CTP / ADP葡萄糖焦磷酸化酶基因的植物细胞的整株植物。

公开(公告)号：US5866775A

公开(公告)日：1999-02-02

申请号：US590647

申请日：1990-09-28

Applicant: David Alan Eichholtz ,  Charles Scott Gasser ,  Ganesh Murthy Kishore

Inventor： David Alan Eichholtz ,  Charles Scott Gasser ,  Ganesh Murthy Kishore

IPC: A01H5/00 ,  C12N5/10 ,  C12N9/10 ,  C12N15/09 ,  C12N15/29 ,  C12N15/31 ,  C12N15/54 ,  C12N15/82 ,  A01H1/06 ,  A01H5/10 ,  C12N5/14 ,  C12N15/52

CPC classification number: C12N9/1092 ,  C12N15/8275 ,  Y10S47/01

Abstract: Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphsate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a first conserved sequence found between positions 80 and 120, and a threonine residue for an alanine residue in a second conserved sequence found between positions 170 and 210 in the mature wild type EPSP synthase.

Abstract translation: 编码耐草甘膦的5-烯醇丙酮酸-3-磷酸鸟苷酸（EPSP）合成酶，编码含有glyphsate的EPSP合成酶的DNA，编码耐草甘膦酶的植物基因，含有该基因的植物转化载体，转化的植物细胞和含有植物基因的分化转化植物 被披露。 草甘膦耐受EPSP合成酶是通过在第80和120位发现的第一个保守序列中的丙氨酸残基取代甘氨酸残基，而在第170和210位发现的第二个保守序列中的丙氨酸残基的苏氨酸残基 成熟野生型EPSP合酶。

公开(公告)号：US5804425A

公开(公告)日：1998-09-08

申请号：US833485

申请日：1997-04-07

Applicant: Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

Inventor： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

IPC: C12N9/10 ,  C12N15/54 ,  C12N15/82 ,  C12N1/00 ,  C12N1/20 ,  C12N15/00

CPC classification number: C12N9/1092 ,  C12N15/8275 ,  Y10S435/816 ,  Y10S435/822 ,  Y10S435/839 ,  Y10S435/874 ,  Y10S435/883 ,  Y10T436/143333

Abstract: Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.

公开(公告)号：US07205457B1

公开(公告)日：2007-04-17

申请号：US08432147

申请日：1994-02-04

Applicant: Ganesh Murthy Kishore ,  Thomas Gene Ruff ,  Vincent Jean-Marie Armel Arondel ,  Susan Irma Gibson ,  Christopher Roland Somerville

Inventor： Ganesh Murthy Kishore ,  Thomas Gene Ruff ,  Vincent Jean-Marie Armel Arondel ,  Susan Irma Gibson ,  Christopher Roland Somerville

IPC: A01H5/00 ,  C12N15/82 ,  C07H21/02

CPC classification number: C12N9/0083 ,  A01H5/10 ,  C12N15/8247 ,  C12Y114/19003

Abstract: Methods of altering the linolenic and oleic acid content in plants are disclosed. Also disclosed are transformed plants which have altered linolenic and oleic acid content, and the DNA molecules used to produce them.

Abstract translation: 公开了改变植物中亚麻酸和油酸含量的方法。 还公开了具有改变的亚麻酸和油酸含量的转化植物以及用于生产它们的DNA分子。

公开(公告)号：US06538179B1

公开(公告)日：2003-03-25

申请号：US08399023

申请日：1995-03-06

Applicant: Gerard Francis Barry ,  Ganesh Murthy Kishore ,  David Martin Stark

Inventor： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  David Martin Stark

IPC: C12N1582

CPC classification number: C12N9/1241 ,  C12N15/8222 ,  C12N15/8235 ,  C12N15/8245 ,  C12N15/8247

Abstract: Promoters for enhanced expression of ADPglucose pyrophosphorylase in potato tubers and fruits such as tomato; methods of using them; DNA molecules, plant cells and plants containing them. A method of decreasing the oil content of seeds by expression of ADPglucose pyrophosphorylase.

Abstract translation: 用于增强马铃薯块茎和水果如番茄中ADP葡萄糖焦磷酸化酶表达的启动子; 使用它们的方法; DNA分子，植物细胞和含有它们的植物。 通过ADP葡萄糖焦磷酸化酶的表达降低种子含油量的方法。

公开(公告)号：US5716837A

公开(公告)日：1998-02-10

申请号：US596024

申请日：1996-02-06

Applicant: Gerard Francis Barry ,  Jan Willem de Weerd ,  Ganesh Murthy Kishore ,  Marcia Lee Weldon

Inventor： Gerard Francis Barry ,  Jan Willem de Weerd ,  Ganesh Murthy Kishore ,  Marcia Lee Weldon

IPC: A01H1/00 ,  C12N5/10 ,  C12N9/10 ,  C12N9/14 ,  C12N15/09 ,  C12N15/29 ,  C12N15/31 ,  C12N15/54 ,  C12N15/82 ,  C12R1/91 ,  A01H4/00 ,  A01H5/00

CPC classification number: C12N9/1051 ,  C12N15/8245

Abstract: Introducing sucrose phosphorylase activity into plants by transformation with a gene for the enzyme increases the rate of sucrose hydrolysis, leading to increased starch, oil, and protein levels. The preferred gene is from Streptococcus mutans. Surprisingly, in potatoes transformed to express this gene in tubers, reduced bruise discoloration susceptibility and increased uniformity of starch deposition throughout the tuber are achieved.

公开(公告)号：US5648249A

公开(公告)日：1997-07-15

申请号：US406858

申请日：1995-03-20

Applicant: Gerard Francis Barry ,  Ganesh Murthy Kishore ,  David Martin Stark ,  James Conrad Zalewski

Inventor： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  David Martin Stark ,  James Conrad Zalewski

IPC: A01H5/00 ,  C12N5/10 ,  C12N9/12 ,  C12N15/09 ,  C12N15/54 ,  C12N15/82 ,  C12N15/31 ,  C12N15/62

CPC classification number: C12N9/1241 ,  C12N15/8245

Abstract: The invention relates to a method of improving the quality of potatoes stored at reduced temperatures and a method of prolonging dormancy of stored potato tubers, by increasing the level of ADPglucose pyrophosphorylase enzyme activity within the potato tuber during storage at ambient or reduced temperatures. Novel DNA molecules, plant cells, and potato plants are provided which contain the gene for the ADPglucose pyrophosphorylase enzyme.

Abstract translation: PCT No.PCT / US94 / 05275第 371日期1995年3月20日 102（e）1995年3月20日PCT PCT 1994年5月18日PCT公布。 第WO94 / 28149号公报 日期1994年12月8日本发明涉及通过在环境储存期间提高马铃薯块茎内的ADP葡萄糖焦磷酸化酶活性的水平，提高在降低温度下储存的马铃薯的质量的方法和延长储存的马铃薯块茎的休眠的方法 降低温度 提供了含有ADP葡萄糖焦磷酸化酶基因的新型DNA分子，植物细胞和马铃薯植物。

公开(公告)号：US06225114B1

公开(公告)日：2001-05-01

申请号：US09243374

申请日：1999-02-01

Applicant: David Alan Eichholtz ,  Charles Scott Gasser ,  Ganesh Murthy Kishore

Inventor： David Alan Eichholtz ,  Charles Scott Gasser ,  Ganesh Murthy Kishore

IPC: C12N1500

CPC classification number: C12N9/1092 ,  C12N15/8275 ,  Y10S47/01

Abstract: DNA encoding glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, plant genes encoding the glyphosate-tolerant enzymes, and plant transformation vectors containing the genes are disclosed. The DNA encodes glyphosate-tolerant EPSP synthases modified by substitution of an alanine residue for a glycine residue in a first conserved sequence found between positions 80 and 120, and a threonine residue for an alanine residue in a second conserved sequence found between positions 170 and 210 in the mature wild type EPSP synthase.

Abstract translation: 公开了编码耐草甘膦的5-烯醇丙酮酸-3-磷酸鸟苷酸（EPSP）合成酶，编码草甘膦耐受酶的植物基因和含有该基因的植物转化载体的DNA。 DNA编码通过在位置80和120之间发现的第一个保守序列中的丙氨酸残基取代甘氨酸残基修饰的草甘膦耐受性EPSP合成酶，以及在位置170和210之间发现的第二个保守序列中的丙氨酸残基的苏氨酸残基 在成熟的野生型EPSP合酶中。

公开(公告)号：US5776760A

公开(公告)日：1998-07-07

申请号：US484274

申请日：1995-06-07

Applicant: Gerard Francis Barry ,  Ganesh Murthy Kishore

Inventor： Gerard Francis Barry ,  Ganesh Murthy Kishore

IPC: A01H5/00 ,  C07H21/04 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/06 ,  C12N15/00 ,  C12N15/09 ,  C12N15/53 ,  C12N15/82 ,  C12P21/06 ,  C12R1/01 ,  C12R1/38 ,  C12R1/91

CPC classification number: C12R1/38 ,  C12N15/8275 ,  C12N9/0004 ,  C12N9/0012 ,  C12R1/01

Abstract: Genes encoding a glyphosate oxidoreductase enzyme are disclosed. The genes are useful in producing transformed bacteria and plants which degrade glyphosate herbicide as well as crop plants which are tolerant to glyphosate herbicide.

公开(公告)号：US5750876A

公开(公告)日：1998-05-12

申请号：US476519

申请日：1995-06-07

Applicant: Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Bradley Martin Krohn

Inventor： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Bradley Martin Krohn

IPC: A01H5/00 ,  C08B30/00 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12N9/28 ,  C12N9/44 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12N15/82 ,  C12P19/04 ,  C12R1/125 ,  C12R1/19 ,  C12R1/91 ,  C12N15/31

CPC classification number: C12N9/246 ,  C12N15/8245 ,  C12N9/1241 ,  C12N9/2451 ,  C12Y302/01068

Abstract: A method of producing plant products containing modified starch content, including higher ratios of amylose to amylopectin, increase in intermediate material, or amylopectin having fewer branches or altered branching pattern. Also provided are DNA constructs and transformed plant cells useful in that method. The preferred method uses isoamylase from a Flavobacterium sp., more preferably in combination with a gene encoding ADPglucose pyrophosphorylase. Also disclosed are the gene from Flavobacterium sp. and transformed bacterial and plant cells containing a derivative thereof.

Abstract translation: 一种生产含有改性淀粉含量的植物产品的方法，其包括较高比例的直链淀粉与支链淀粉，中间物质的增加或具有较少枝条或改变的分枝花样的支链淀粉。 还提供了在该方法中有用的DNA构建体和转化的植物细胞。 优选的方法使用来自黄杆菌属的异淀粉酶，更优选与编码ADP葡萄糖焦磷酸化酶的基因组合。 还公开了来自黄杆菌属（Flavobacterium sp。）的基因。 并转化含有其衍生物的细菌和植物细胞。