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公开(公告)号：US09321996B2
公开(公告)日：2016-04-26
申请号：US13608803
申请日：2012-09-10
申请人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David Epstein , Dale Gruber , Don McClure , Paul Price
发明人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David Epstein , Dale Gruber , Don McClure , Paul Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
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公开(公告)号：US08455246B2
公开(公告)日：2013-06-04
申请号：US12463351
申请日：2009-05-08
申请人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David A. Epstein , Dale Gruber , Paul Price
发明人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David A. Epstein , Dale Gruber , Paul Price
IPC分类号： A61K33/32 , A61K33/34 , A01N59/16 , C12N5/00 , C12N5/02 , C12N5/07 , C12N5/10 , C12N11/00 , C12N11/16
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。
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公开(公告)号：US20060148074A1
公开(公告)日：2006-07-06
申请号：US11151647
申请日：2005-06-14
申请人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David Epstein , Dale Gruber , Don McClure , Paul Price
发明人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David Epstein , Dale Gruber , Don McClure , Paul Price
IPC分类号： C12N5/06
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
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公开(公告)号：US08785194B2
公开(公告)日：2014-07-22
申请号：US12463371
申请日：2009-05-08
申请人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul Price
发明人： Stephen Gorfien , Richard Fike , Glenn Godwin , Joyce Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。
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