公开(公告)号：US08785194B2

公开(公告)日：2014-07-22

申请号：US12463371

申请日：2009-05-08

申请人： Stephen Gorfien ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David A. Epstein ,  Dale Gruber ,  Don McClure ,  Paul Price

发明人： Stephen Gorfien ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David A. Epstein ,  Dale Gruber ,  Don McClure ,  Paul Price

IPC分类号： A61K33/00 ,  A61K33/26 ,  A61K33/32 ,  C12N5/00 ,  C12N5/02 ,  C12N5/07 ,  C12N5/10

CPC分类号： C12N5/0682 ,  C07K14/61 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0603 ,  C12N7/00 ,  C12N9/2402 ,  C12N9/2471 ,  C12N15/85 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2710/10051 ,  C12Y302/01023

摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。

公开(公告)号：US20060148074A1

公开(公告)日：2006-07-06

申请号：US11151647

申请日：2005-06-14

申请人： Stephen Gorfien ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David Epstein ,  Dale Gruber ,  Don McClure ,  Paul Price

发明人： Stephen Gorfien ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David Epstein ,  Dale Gruber ,  Don McClure ,  Paul Price

IPC分类号： C12N5/06

CPC分类号： C12N5/0682 ,  C07K14/61 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0603 ,  C12N7/00 ,  C12N9/2402 ,  C12N9/2471 ,  C12N15/85 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2710/10051 ,  C12Y302/01023

摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

公开(公告)号：US08455246B2

公开(公告)日：2013-06-04

申请号：US12463351

申请日：2009-05-08

申请人： Stephen Gorfien ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David A. Epstein ,  Dale Gruber ,  Paul Price

发明人： Stephen Gorfien ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David A. Epstein ,  Dale Gruber ,  Paul Price

IPC分类号： A61K33/32 ,  A61K33/34 ,  A01N59/16 ,  C12N5/00 ,  C12N5/02 ,  C12N5/07 ,  C12N5/10 ,  C12N11/00 ,  C12N11/16

CPC分类号： C12N5/0682 ,  C07K14/61 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0603 ,  C12N7/00 ,  C12N9/2402 ,  C12N9/2471 ,  C12N15/85 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2710/10051 ,  C12Y302/01023

摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。

公开(公告)号：US09321996B2

公开(公告)日：2016-04-26

申请号：US13608803

申请日：2012-09-10

申请人： Stephen Gorfien ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David Epstein ,  Dale Gruber ,  Don McClure ,  Paul Price

发明人： Stephen Gorfien ,  Richard Fike ,  Glenn Godwin ,  Joyce Dzimian ,  David Epstein ,  Dale Gruber ,  Don McClure ,  Paul Price

IPC分类号： C12N5/071 ,  C07K14/61 ,  C12N7/00 ,  C12N15/85 ,  C12N9/38 ,  C12N5/073 ,  C12N9/24 ,  C12N5/00

CPC分类号： C12N5/0682 ,  C07K14/61 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0603 ,  C12N7/00 ,  C12N9/2402 ,  C12N9/2471 ,  C12N15/85 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/95 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2710/10051 ,  C12Y302/01023

摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

公开(公告)号：US20130084640A1

公开(公告)日：2013-04-04

申请号：US13603270

申请日：2012-09-04

申请人： Paul PRICE ,  Stephen Gorfien ,  Douglas Danner ,  Mark Plavsic

发明人： Paul PRICE ,  Stephen Gorfien ,  Douglas Danner ,  Mark Plavsic

IPC分类号： C12N5/07

CPC分类号： C12N5/0043 ,  C12N5/06 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/40 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2501/11

摘要： The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production.

摘要翻译： 本发明提供能够支持体外培养动物细胞的无血清细胞培养基制剂。 培养基包含至少一种非动物衍生营养素，例如至少一种植物肽和/或至少一种非动物或植物脂质和/或脂肪酸。 培养基还可以任选地包含酵母细胞的酶促消化或提取物。 本发明还提供了使用这些细胞培养基制剂在体外培养动物细胞的方法。 此外，本发明的培养基可以用于病毒生产的动物细胞的生长。