Pharmaceutical compositions and topical use thereof

    公开(公告)号:US09730964B2

    公开(公告)日:2017-08-15

    申请号:US14005269

    申请日:2012-03-15

    CPC分类号: A61K35/35 A61K35/28

    摘要: The present invention relates to methods for the preparation of compositions comprising adipose tissue-derived secretions, for example those derived from bovine adipose tissue, and the use of such compositions in the preparation of a pharmaceutical composition for topical use. The invention also relates to the use of adipose tissue-derived secretions and pharmaceutical compositions thereof for the topical treatment of a non-inflammatory condition, for example the treatment of a skin condition, and for the stimulation of hair growth in a subject by topical application. The invention also relates to the use of adipose tissue-derived secretions and pharmaceutical compositions thereof for the topical treatment of acne.

    Method for the detection of viable Cryptosporidium parvum oocysts
    5.
    发明授权
    Method for the detection of viable Cryptosporidium parvum oocysts 失效
    检测活菌隐孢子虫卵囊的方法

    公开(公告)号:US06146855A

    公开(公告)日:2000-11-14

    申请号:US952376

    申请日:1998-03-03

    IPC分类号: C12Q1/68 C07H21/04 C12P19/34

    CPC分类号: C12Q1/6893 C12Q1/6841

    摘要: Oligonucleotide molecules and methods are disclosed for the detection of viable oocysts or other cells of the protozoa species, Cyrptosporidium parvum. Preferred oligonucleotide molecules are selected from the group comprising oligonucleotides having one or more of the following sequences: (a) ACA ATT ATT, (b) CTT TTT GGT, (c) ATT TTA TAT AAA ATA TTT TGA TGA A, (d) TTT TTT TTT TTA GTA T, (e) TAT ATT TTT TAT CTG, (f) CTT TAC TTA CAT GGA TAA CCG, or comprising a part of the sequences (a) to (f) above so as to allow specific hybridization to unique 18S rRNA sequences of C. parvum.

    摘要翻译: PCT No.PCT / AU96 / 00274 Sec。 371日期1998年3月3日 102(e)1998年3月3日PCT PCT 1996年5月6日PCT公布。 出版物WO96 / 34978 日期1996年11月7日公开了寡核苷酸分子和方法用于检测原生动物物种Cyrptosporidium parvum的活卵囊或其它细胞。 优选的寡核苷酸分子选自具有一个或多个以下序列的寡核苷酸:(a)ACA ATT ATT,(b)CTT TTT GGT,(c)ATT TTA TAT AAA ATA TTT TGA TGA A,(d)TTT TTT TTT TTA GTA T,(e)TAT ATT TTT TAT CTG,(f)CTT TAC TTA CAT GGA TAA CCG,或包含上述序列(a)至(f)的一部分,以便允许与独特的18S的特异性杂交 小曲霉的rRNA序列。

    Therapeutic methods using adipose tissue-derived cell suspensions comprising adipocytes
    7.
    发明授权
    Therapeutic methods using adipose tissue-derived cell suspensions comprising adipocytes 有权
    使用包含脂肪细胞的脂肪组织来源的细胞悬液的治疗方法

    公开(公告)号:US09062288B2

    公开(公告)日:2015-06-23

    申请号:US13059646

    申请日:2009-08-20

    申请人: Graham Vesey

    发明人: Graham Vesey

    IPC分类号: A61K35/35 C12N5/077 A61K35/12

    摘要: Provided herein is a use of an adipose tissue-derived cell suspension which comprises adipocytes for the preparation of a pharmaceutical composition for use in the treatment of an inflammatory disorder, a cartilage or bone disorder and/or the alleviation of pain associated with an inflammatory disorder in a mammalian subject. Also provided herein is a method of treating an inflammatory disorder, a cartilage or bone disorder or alleviating pain associated with an inflammatory disorder in a mammalian subject, comprising administering to the subject a pharmaceutical composition which comprises: (i) an adipose tissue-derived cell suspension which comprises adipocytes; or (ii) a cell-free extract which is prepared from an adipose tissue-derived cell suspension, wherein the adipose tissue-derived cell suspension comprises adipocytes, together with a pharmaceutically-acceptable carrier or diluent.

    摘要翻译: 本文提供了脂肪组织来源的细胞悬浮液的用途,其包含脂肪细胞用于制备用于治疗炎症性疾病,软骨或骨骼疾病和/或缓解与炎性疾病相关的疼痛的药物组合物 在哺乳动物受试者。 本文还提供了治疗炎症性疾病,软骨或骨骼疾病或减轻与哺乳动物受试者中的炎性病症相关的疼痛的方法,其包括向受试者施用药物组合物,其包含:(i)脂肪组织来源的细胞 包含脂肪细胞的悬浮液; 或(ii)由脂肪组织衍生的细胞悬浮液制备的无细胞提取物,其中所述脂肪组织来源的细胞悬液包含脂肪细胞,以及药学上可接受的载体或稀释剂。

    Products comprising quantum of bioparticles and method for production thereof
    8.
    发明授权
    Products comprising quantum of bioparticles and method for production thereof 有权
    包含生物粒子量的产品及其生产方法

    公开(公告)号:US06780581B2

    公开(公告)日:2004-08-24

    申请号:US09949860

    申请日:2001-09-12

    IPC分类号: C12Q170

    CPC分类号: A01N1/02 A01N1/0221 C12N1/04

    摘要: A method for the production of a product containing a quantum of bioparticles, for example bacterial cells, is provided, as are products obtained thereby. In one embodiment, the products contain a defined quantum of bioparticles, preferably presented in a viable state. Products made in accordance with the invention may be usefully put to applications where having knowledge of the number of bioparticles present may be important or at least advantageous.

    摘要翻译: 提供了生产包含量子生物颗粒例如细菌细胞的产品的方法,以及由此得到的产品。 在一个实施方案中,产物含有限定量的生物颗粒,优选呈现在可行状态。 根据本发明制备的产品可以有用地投入应用,其中了解存在的生物颗粒的数量可能是重要的或至少是有利的。

    Antibodies to cryptosporidium
    9.
    发明授权
    Antibodies to cryptosporidium 有权
    隐孢子虫的抗体

    公开(公告)号:US06777222B1

    公开(公告)日:2004-08-17

    申请号:US09424048

    申请日:2000-02-24

    IPC分类号: C12N110

    摘要: A method of producing IgG1 subclass antibodies reactive to the surface of Crystosporidium oocysts, the method comprising: (a) separating at least a protion of the Cryptosporidium oocyst wall from the internal sporozoites to form an oocyst-wall preparation; (b) treating the separated oocyst-wall preparation so as to obtain an oocyst antigen preparation capable of eliciting a detectable IgG1 immune response in an animal to the surface of the oocyst; (c) immunizing an animal with the oocyst antigen preparation so as to elicit an IgG1 immune response in the animal; and (d) obtaining from the animal IgG1 antibodies reactive to the surface of Cryptosporidium oocysts. IgG1 antibodies reactive to the surface of Cryptosporidium oocysts.

    摘要翻译: 一种产生对Crystosporidium oocysts表面具有反应性的IgG1亚类抗体的方法,该方法包括:(a)从内部子孢子中分离出至少一种隐孢子虫卵囊壁,形成卵囊壁制剂; (b)处理分离的卵囊壁制剂以获得能够在动物体内向卵囊表面引发可检测的IgG1免疫应答的卵囊抗原制剂; (c)用卵囊抗原制剂免疫动物以在动物中引发IgG1免疫应答; 和(d)从动物IgG1获得对隐孢子虫卵囊表面有反应性的抗体。 IgG1抗体与隐孢子虫卵囊表面反应。

    Method for detecting microorganisms
    10.
    发明授权
    Method for detecting microorganisms 失效
    微生物检测方法

    公开(公告)号:US06225046B1

    公开(公告)日:2001-05-01

    申请号:US08942206

    申请日:1998-07-28

    IPC分类号: G01N1102

    摘要: Methods for detecting microorganisms in a sample by binding detectable particles and fluorescent labelled ligands reactive to the microorganisms. The present invention also includes the use of multiple fluorochromes for the detection of microorganisms and is adaptable for use in flow cytometry.

    摘要翻译: 通过结合可检测的颗粒和与微生物反应的荧光标记配体来检测样品中的微生物的方法。 本发明还包括使用多种荧光染料来检测微生物并且适用于流式细胞术。