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    Norovirus detection, methods and compositions therefor
    1.
    发明授权
    Norovirus detection, methods and compositions therefor 有权
    诺如病毒检测，其方法和组合物

    公开(公告)号：US07794928B2

    公开(公告)日：2010-09-14

    申请号：US12025327

    申请日：2008-02-04

    申请人： Herbert W. Virgin ,  Christiane Wobus ,  Stephanie Karst

    发明人： Herbert W. Virgin ,  Christiane Wobus ,  Stephanie Karst

    IPC分类号： C12Q1/70 ,  C12P21/04 ,  C12N5/00 ,  C12N7/00 ,  G01N33/567

    CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K16/10 ,  C07K2317/76 ,  C12N15/1131 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/52 ,  C12N2770/16022 ,  C12N2770/16051 ,  C12N2310/3521

    摘要： A norovirus-permissive cell culture infected with a norovirus, and methods of culturing a norovirus, are disclosed. Norovirus-permissive cells include dendritic cell-lineage cells, and macrophage-lineage cells, such as dendritic cells, and macrophages having a deficiency in a cellular anti-viral pathway such as a STAT-1-dependent pathway, an interferon receptor-dependent pathway, or a PKR-dependent pathway. Also disclosed are methods of screening anti-viral compounds against norovirus-permissive cells infected with norovirus, and norovirus adapted to grow in fibroblasts as well as macrophages that are not deficient in a cellular anti-viral pathway. Methods of making a norovirus vaccine are also disclosed. A replicative form of norovirus as well as its use in the development of an anti-viral agent and a polypeptide expression system are also described. Further disclosed are methods of detecting norovirus in a sample.

    摘要翻译： 公开了用诺如病毒感染的诺如病毒允许细胞培养物，以及培养诺如病毒的方法。 诺如病毒允许细胞包括树突状细胞谱系细胞和巨噬细胞谱系细胞，例如树突细胞和在细胞抗病毒途径中缺乏的巨噬细胞，例如STAT-1-依赖性途径，干扰素受体依赖性途径 ，或PKR依赖性途径。 还公开了筛选针对诺如病毒感染的诺如病毒允许细胞的抗病毒化合物的方法，以及适于在成纤维细胞以及细胞抗病毒途径中不缺陷的巨噬细胞生长的诺如病毒。 还公开了制备诺如病毒疫苗的方法。 还描述了诺如病毒的复制形式及其在开发抗病毒剂和多肽表达系统中的用途。 还公开了检测样品中的诺如病毒的方法。

    Norovirus detection, methods and compositions therefor
    3.
    发明申请
    Norovirus detection, methods and compositions therefor 有权
    诺如病毒检测，其方法和组合物

    公开(公告)号：US20080254443A1

    公开(公告)日：2008-10-16

    申请号：US12025327

    申请日：2008-02-04

    申请人： Herbert W. Virgin ,  Christiane Wobus ,  Stephanie Karst

    发明人： Herbert W. Virgin ,  Christiane Wobus ,  Stephanie Karst

    IPC分类号： C12Q1/70

    CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K16/10 ,  C07K2317/76 ,  C12N15/1131 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/52 ,  C12N2770/16022 ,  C12N2770/16051 ,  C12N2310/3521

    摘要： A norovirus-permissive cell culture infected with a norovirus, and methods of culturing a norovirus, are disclosed. Norovirus-permissive cells include dendritic cell-lineage cells, and macrophage-lineage cells, such as dendritic cells, and macrophages having a deficiency in a cellular anti-viral pathway such as a STAT-1-dependent pathway, an interferon receptor-dependent pathway, or a PKR-dependent pathway. Also disclosed are methods of screening anti-viral compounds against norovirus-permissive cells infected with norovirus, and norovirus adapted to grow in fibroblasts as well as macrophages that are not deficient in a cellular anti-viral pathway. Methods of making a norovirus vaccine are also disclosed. A replicative form of norovirus as well as its use in the development of an anti-viral agent and a polypeptide expression system are also described. Further disclosed are methods of detecting norovirus in a sample.

    摘要翻译： 公开了用诺如病毒感染的诺如病毒允许细胞培养物，以及培养诺如病毒的方法。 诺如病毒允许细胞包括树突状细胞谱系细胞和巨噬细胞谱系细胞，例如树突细胞和在细胞抗病毒途径中缺乏的巨噬细胞，例如STAT-1-依赖性途径，干扰素受体依赖性途径 ，或PKR依赖性途径。 还公开了筛选针对诺如病毒感染的诺如病毒允许细胞的抗病毒化合物的方法，以及适于在成纤维细胞以及细胞抗病毒途径中不缺陷的巨噬细胞生长的诺如病毒。 还公开了制备诺如病毒疫苗的方法。 还描述了诺如病毒的复制形式及其在开发抗病毒剂和多肽表达系统中的用途。 还公开了检测样品中的诺如病毒的方法。

    METHODS OF PREVENTING AND TREATING VIRAL INFECTIONS BY INHIBITING THE DelSGYLATION ACTIVITY OF OTU DOMAIN-CONTAINING VIRAL PROTEINS
    6.
    发明申请
    METHODS OF PREVENTING AND TREATING VIRAL INFECTIONS BY INHIBITING THE DelSGYLATION ACTIVITY OF OTU DOMAIN-CONTAINING VIRAL PROTEINS 有权
    通过抑制包含OTU域的病毒蛋白质的去除活性来预防和治疗病毒感染的方法

    公开(公告)号：US20110033498A1

    公开(公告)日：2011-02-10

    申请号：US12594774

    申请日：2008-04-07

    申请人： Adolfo Garcia-Sastre ,  Natalia Frias-Staheli ,  Herbert W. Virgin ,  Nadia Giannakopoulos

    发明人： Adolfo Garcia-Sastre ,  Natalia Frias-Staheli ,  Herbert W. Virgin ,  Nadia Giannakopoulos

    IPC分类号： A61K39/12 ,  C12N7/01 ,  C12N7/02 ,  G01N33/53

    CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N2760/12021 ,  C12N2760/12022 ,  C12N2770/10022

    摘要： Viruses having an impaired ability to deISGylate ISG15 conjugates, in particular, viral mutants comprising a mutation in the viral genome that reduces or eliminates the ability of the viral OTU domain-containing protein encoded by the viral genome to deISGylate ISG15 conjugates and/or deubiquitinate ubiquitinated proteins and/or deNeddylate Neddylated proteins are disclosed. Such viral mutants may be used in the formulation of immunogenic compositions for inducing an immune response and preventing, managing and/or treating a viral infection. Also disclosed are methods for identifying anti-viral compounds, in particular, methods of identifying compounds that reduce or inhibit the deISGylation activity and/or deubiquitination and/or deNeddylation activity of a viral OTU domain-containing protein. The compounds identified using such methods may be used as antiviral agents for the prevention, treatment and/or management of viral infections.

    摘要翻译： 具有减弱ISG15缀合物脱敏能力的病毒，特别是包含病毒基因组中的突变的病毒突变体，其降低或消除由病毒基因组编码的病毒OTU结构域的蛋白质使ISG15缀合物和/或泛素化泛素化的能力脱落 公开了蛋白质和/或脱硝化二烯酸化蛋白质。 这样的病毒突变体可用于诱导免疫应答和预防，控制和/或治疗病毒感染的免疫原性组合物的制剂中。 还公开了用于鉴定抗病毒化合物的方法，特别是鉴定降低或抑制含有病毒OTU结构域的蛋白质的脱甲硅烷基化活性和/或去泛素化和/或脱亚氨基化活性的化合物的方法。 使用这些方法鉴定的化合物可用作预防，治疗和/或管理病毒感染的抗病毒剂。

    Norovirus infected cell cultures and uses therefor
    7.
    发明授权
    Norovirus infected cell cultures and uses therefor 有权
    诺如病毒感染细胞培养物及其用途

    公开(公告)号：US07264923B2

    公开(公告)日：2007-09-04

    申请号：US10839211

    申请日：2004-05-05

    申请人： Herbert W. Virgin ,  Christiane Wobus ,  Stephanie Karst

    发明人： Herbert W. Virgin ,  Christiane Wobus ,  Stephanie Karst

    IPC分类号： C12Q1/70 ,  C12N5/00 ,  C12N5/02 ,  C12N7/00 ,  C12N7/02 ,  C12N5/06 ,  C12P21/04

    CPC分类号： C07K14/005 ,  A61K2039/5254 ,  C12N7/00 ,  C12N2770/30021 ,  C12N2770/30022

    摘要： A norovirus-permissive cell culture infected with a norovirus, and methods of culturing a norovirus, are disclosed. Norovirus-permissive cells include dendritic cell-lineage cells, and macrophage-lineage cells, such as dendritic cells, and macrophages having a deficiency in a cellular anti-viral pathway such as a STAT-1-dependent pathway, an interferon receptor-dependent pathway, or a PKR-dependent pathway. Also disclosed are methods of screening anti-viral compounds against norovirus-permissive cells infected with norovirus, and norovirus adapted to grow in fibroblasts as well as macrophages that are not deficient in a cellular anti-viral pathway. Methods of making a norovirus vaccine are also disclosed.

    摘要翻译： 公开了用诺如病毒感染的诺如病毒允许细胞培养物，以及培养诺如病毒的方法。 诺如病毒允许细胞包括树突状细胞谱系细胞和巨噬细胞谱系细胞，例如树突细胞和在细胞抗病毒途径中缺乏的巨噬细胞，例如STAT-1-依赖性途径，干扰素受体依赖性途径 ，或PKR依赖性途径。 还公开了筛选针对诺如病毒感染的诺如病毒允许细胞的抗病毒化合物的方法，以及适于在成纤维细胞以及细胞抗病毒途径中不缺陷的巨噬细胞生长的诺如病毒。 还公开了制备诺如病毒疫苗的方法。

    Methods of preventing and treating viral infections by inhibiting the deISGylation activity of OTU domain-containing viral proteins
    10.
    发明授权
    Methods of preventing and treating viral infections by inhibiting the deISGylation activity of OTU domain-containing viral proteins 有权
    通过抑制含有OTU结构域的病毒蛋白的deISG化活性来预防和治疗病毒感染的方法

    公开(公告)号：US08709730B2

    公开(公告)日：2014-04-29

    申请号：US12594774

    申请日：2008-04-07

    申请人： Adolfo Garcia-Sastre ,  Natalia Frias-Staheli ,  Herbert W. Virgin ,  Nadia Vicki Giannakopoulos

    发明人： Adolfo Garcia-Sastre ,  Natalia Frias-Staheli ,  Herbert W. Virgin ,  Nadia Vicki Giannakopoulos

    IPC分类号： G01N33/53 ,  A61K39/12

    CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N2760/12021 ,  C12N2760/12022 ,  C12N2770/10022

    摘要： Viruses having an impaired ability to deISGylate ISG15 conjugates, in particular, viral mutants comprising a mutation in the viral genome that reduces or eliminates the ability of the viral OTU domain-containing protein encoded by the viral genome to deISGylate ISG15 conjugates and/or deubiquitinate ubiquitinated proteins and/or deNeddylate Neddylated proteins are disclosed. Such viral mutants may be used in the formulation of immunogenic compositions for inducing an immune response and preventing, managing and/or treating a viral infection. Also disclosed are methods for identifying anti-viral compounds, in particular, methods of identifying compounds that reduce or inhibit the deISGylation activity and/or deubiquitination and/or deNeddylation activity of a viral OTU domain-containing protein. The compounds identified using such methods may be used as antiviral agents for the prevention, treatment and/or management of viral infections.

    摘要翻译： 具有减弱ISG15缀合物脱敏能力的病毒，特别是包含病毒基因组中的突变的病毒突变体，其降低或消除由病毒基因组编码的病毒OTU结构域的蛋白质使ISG15缀合物和/或泛素化泛素化的能力脱落 公开了蛋白质和/或脱硝化二烯酸化蛋白质。 这样的病毒突变体可用于诱导免疫应答和预防，控制和/或治疗病毒感染的免疫原性组合物的制剂中。 还公开了用于鉴定抗病毒化合物的方法，特别是鉴定降低或抑制含有病毒OTU结构域的蛋白质的脱甲硅烷基化活性和/或去泛素化和/或脱亚氨基化活性的化合物的方法。 使用这些方法鉴定的化合物可用作预防，治疗和/或管理病毒感染的抗病毒剂。