公开(公告)号：US08980625B2

公开(公告)日：2015-03-17

申请号：US11360535

申请日：2006-02-24

申请人： Yuji Kikuchi ,  Hiroko Kikuchi ,  Taiji Nishi ,  Takenori Kitani ,  Yukihiro Yanagawa ,  Motohiro Fukuda

发明人： Yuji Kikuchi ,  Hiroko Kikuchi ,  Taiji Nishi ,  Takenori Kitani ,  Yukihiro Yanagawa ,  Motohiro Fukuda

IPC分类号： C12M1/22 ,  C12M3/00 ,  C12M1/12 ,  C12M1/00 ,  C12M3/06 ,  B01L3/00

CPC分类号： C12M23/16 ,  B01L3/5027

摘要： A cell culture plate has a plurality of flow channels, uneven pattern areas and through holes. The flow channels are formed between the uneven pattern areas, and culture solution flows from the flow channels into the uneven pattern areas or from the uneven pattern areas into the flow channels. The uneven pattern area has uneven patterns that create a cell culture space.

摘要翻译： 细胞培养板具有多个流路，不均匀图案区域和通孔。 流动通道形成在不均匀图案区域之间，培养液从流动通道流入不均匀图案区域或从凹凸图案区域流入流动通道。 不均匀图案区域具有形成细胞培养空间的不均匀图案。

公开(公告)号：US20090280197A1

公开(公告)日：2009-11-12

申请号：US12373725

申请日：2007-09-04

申请人： Hiroko Kikuchi ,  Masaki Arashima ,  Sayaka Aramaki ,  Hiroko Yoshida

发明人： Hiroko Kikuchi ,  Masaki Arashima ,  Sayaka Aramaki ,  Hiroko Yoshida

IPC分类号： A61K36/00

CPC分类号： A61K8/97 ,  A23L33/105 ,  A23V2002/00 ,  A61K2800/522 ,  A61Q19/02 ,  A61Q19/08 ,  A23V2250/21

摘要： The present invention provides a cell activation agent, a collagen production accelerator, a whitening agent, an antioxidant, an anti-inflammatory agent, an aromatase activity accelerator, a protease activity accelerator, a skin external preparation, and food, which all contain an extract of Hibiscus makinoi.

摘要翻译： 本发明提供一种细胞活化剂，胶原产生促进剂，增白剂，抗氧化剂，抗炎剂，芳香酶活性促进剂，蛋白酶活性促进剂，皮肤外用剂和食品，它们都含有提取物 的木槿。

公开(公告)号：US07794759B2

公开(公告)日：2010-09-14

申请号：US12373725

申请日：2007-09-04

申请人： Hiroko Kikuchi ,  Masaki Arashima ,  Sayaka Aramaki ,  Hiroko Yoshida

发明人： Hiroko Kikuchi ,  Masaki Arashima ,  Sayaka Aramaki ,  Hiroko Yoshida

IPC分类号： A61K36/00

CPC分类号： A61K8/97 ,  A23L33/105 ,  A23V2002/00 ,  A61K2800/522 ,  A61Q19/02 ,  A61Q19/08 ,  A23V2250/21

摘要： The present invention provides a cell activation agent, a collagen production accelerator, a whitening agent, an antioxidant, an anti-inflammatory agent, an aromatase activity accelerator, a protease activity accelerator, a skin external preparation, and food, which all contain an extract of Hibiscus makinoi.

摘要翻译： 本发明提供一种细胞活化剂，胶原产生促进剂，增白剂，抗氧化剂，抗炎剂，芳香酶活性促进剂，蛋白酶活性促进剂，皮肤外用剂和食品，它们都含有提取物 的木槿。

公开(公告)号：US20070202589A1

公开(公告)日：2007-08-30

申请号：US11360535

申请日：2006-02-24

申请人： Yuji Kikuchi ,  Hiroko Kikuchi ,  Taiji Nishi ,  Takenori Kitani ,  Yukihiro Yanagawa ,  Motohiro Fukuda

发明人： Yuji Kikuchi ,  Hiroko Kikuchi ,  Taiji Nishi ,  Takenori Kitani ,  Yukihiro Yanagawa ,  Motohiro Fukuda

IPC分类号： C12M3/00

CPC分类号： C12M23/16 ,  B01L3/5027

摘要： A cell culture plate has a plurality of flow channels, uneven pattern areas and through holes. The flow channels are formed between the uneven pattern areas, and culture solution flows from the flow channels into the uneven pattern areas or from the uneven pattern areas into the flow channels. The uneven pattern area has uneven patterns that create a cell culture space.

摘要翻译： 细胞培养板具有多个流路，不均匀图案区域和通孔。 流动通道形成在不均匀图案区域之间，培养液从流动通道流入不均匀图案区域或从凹凸图案区域流入流动通道。 不均匀图案区域具有形成细胞培养空间的不均匀图案。

公开(公告)号：US20070184432A1

公开(公告)日：2007-08-09

申请号：US11691815

申请日：2007-03-27

申请人： Shiro KANEGASAKI ,  Yuji Kikuchi ,  Hiroko Kikuchi

发明人： Shiro KANEGASAKI ,  Yuji Kikuchi ,  Hiroko Kikuchi

IPC分类号： C12Q1/00

CPC分类号： B01L3/502715 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2400/086 ,  G01N15/1456 ,  G01N2015/1486

摘要： The present invention aims at providing a structure whereby, in the step of injecting a sample in a microquantity into a well, the migration of the sample into another well or overflow thereof can be prevented; and a structure whereby the position of the injected sample can be adjusted in a well or the sample can be transferred into the next well under controlling. It also aims at providing an apparatus for detecting chemotaxis of cells and separating chemotactic cells with the application of the above structures. Accordingly, the present invention provides a microsample treatment apparatus and an apparatus for detecting chemotaxis of cells and separating chemotactic cells wherein a plural number of wells are connected to each other via a part having resistance to fluids and the wells are each provided with tubes for injecting/sucking a sample and, if necessary, tubes for relieving pressure changes at the injection/suction, characterized in that these tubes have a space in common at the top ends thereof in which a liquid can be held.

摘要翻译： 本发明的目的在于提供一种结构，其中在将微量样品注入井中的步骤中，可以防止样品向另一个井或其溢流的迁移; 并且可以在井中调整注入的样品的位置，或者可以在控制下将样品转移到下一个孔中的结构。 它还旨在提供一种用于检测细胞的趋化性并分离趋化细胞并利用上述结构的装置。 因此，本发明提供了一种微量样品处理装置和用于检测细胞趋化性和分离趋化细胞的微量样品处理装置和装置，其中通过具有耐流体性的部分将多个孔相互连接，并且各孔分别设置有用于注射的管 抽吸样品，如果需要，用于在注射/抽吸时减轻压力变化的管，其特征在于，这些管在其顶端具有共同的空间，其中可以保持液体。

公开(公告)号：US07022516B2

公开(公告)日：2006-04-04

申请号：US10181708

申请日：2001-12-06

申请人： Shiro Kanegasaki ,  Yuji Kikuchi ,  Hiroko Kikuchi

发明人： Shiro Kanegasaki ,  Yuji Kikuchi ,  Hiroko Kikuchi

IPC分类号： C12M1/34

CPC分类号： G01N33/5302 ,  B01L3/5027 ,  B01L3/502761 ,  B01L2300/0861 ,  B01L2400/086 ,  C12M41/46 ,  G01N15/1456 ,  G01N33/5005 ,  G01N2015/1486 ,  G01N2015/1493

摘要： The present invention aims at providing a well unit to be used in fabricating an apparatus whereby movements of cells based on their own actions can be accurately and easily detected, in detecting the chemotaxis of cells due to a chemotactic factor or the inhibition of the chemotaxis of cells by an inhibitor.Accordingly, the present invention provides a well unit to be used in an apparatus for detecting chemotaxis of cells and separating cells characterized in that a plural number of wells, in which a liquid sample can be held in a resting state, are connected to each other via a channel, the channel is provided with a bank, and, in the upper part of the bank, barriers constituting one or more grooves having a width and/or a depth fit for the diameter or deformability of cells are provided or a plane is provided so as to give a gap having a depth fit for the diameter or deformability of cells between the plane and the glass substrate.