公开(公告)号：US5597688A

公开(公告)日：1997-01-28

申请号：US467799

申请日：1995-06-06

申请人： Mark C. Connelly ,  Utpal R. Chakraborty ,  Houston G. Brooks, Jr.

发明人： Mark C. Connelly ,  Utpal R. Chakraborty ,  Houston G. Brooks, Jr.

IPC分类号： C12Q1/00 ,  G01N1/30 ,  G01N15/14 ,  G01N33/48 ,  G01N33/533 ,  G01N33/545 ,  G01N33/569 ,  C12Q1/70 ,  G01N33/53

CPC分类号： G01N33/56988 ,  G01N1/30 ,  G01N33/56972 ,  G01N33/56983 ,  G01N2001/305 ,  Y10S435/975 ,  Y10S436/805 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25

摘要： The present invention provides a novel cellular fixative composition and method of cellular fixing and cellular analysis wherein cells may be fixed for further analysis without destroying the cell's surface markers, cellular morphology, and the cell's light scattering properties. Treatment of cells with the fixative as described herein also allows antibodies or .other desired components to enter the cell through the cellular membrane, without allowing important contents of the cell to escape. As an added benefit, the fixative composition of the invention may also kill or at least inactivate or reduce the activity of deadly virus contained in the cell. In its broadest aspect, the presently claimed fixative composition comprises at least three components, and in more preferred embodiments, four components. In a particularly preferred embodiment, the fixative composition described herein comprises at least one compound suitable for increasing the permeability of a cellular membrane, at least one compound that facilitates transportation of components across cellular membranes, at least one detergent, and at least one compound having the structure:R.sub.1 R.sub.2 R.sub.3 R.sub.4 R.sub.5 --Ar--X

摘要翻译： 本发明提供了一种新的细胞固定组合物和细胞固定和细胞分析方法，其中细胞可以固定用于进一步分析，而不破坏细胞的表面标志物，细胞形态和细胞的光散射性质。 用本文所述的固定剂处理细胞还允许抗体或其它所需组分通过细胞膜进入细胞，而不允许细胞的重要内容物逸出。 作为额外的益处，本发明的固定组合物还可以杀死或至少灭活或降低细胞中包含的致命病毒的活性。 在其最广泛的方面，目前要求的固定剂组合物包含至少三种组分，在更优选的实施方案中包含四种组分。 在一个特别优选的实施方案中，本文描述的固定剂组合物包含至少一种适合于增加细胞膜的渗透性的化合物，至少一种促进组分跨细胞膜运输的化合物，至少一种洗涤剂和至少一种具有 结构：R1R2R3R4R5-Ar-X

公开(公告)号：US5571726A

公开(公告)日：1996-11-05

申请号：US446533

申请日：1995-05-19

申请人： Houston G. Brooks, Jr. ,  Chi-Deu Chang ,  Utpal R. Chakraborty ,  Henry A. Graham, Jr. ,  Lloyd L. Hollenbeck, Jr. ,  Sharon R. Lawler ,  Jennifer Nasser ,  Ernest G. Schutt ,  Albert Venturini

发明人： Houston G. Brooks, Jr. ,  Chi-Deu Chang ,  Utpal R. Chakraborty ,  Henry A. Graham, Jr. ,  Lloyd L. Hollenbeck, Jr. ,  Sharon R. Lawler ,  Jennifer Nasser ,  Ernest G. Schutt ,  Albert Venturini

IPC分类号： B01J13/00 ,  G01N33/543 ,  G01N33/558 ,  G01N33/58 ,  G01N33/74 ,  G01N33/551 ,  G01N33/553

CPC分类号： G01N33/587 ,  B01J13/0004 ,  B01J13/0043 ,  G01N33/54326 ,  G01N33/54386 ,  G01N33/558 ,  G01N33/74 ,  Y10S435/975

摘要： A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.

摘要翻译： 用于各种免疫测定系统的方法和测试试剂盒。 提供一种制备含有预选尺寸的金属溶胶颗粒的金属溶胶试剂的方法。 在优化的pH条件下还原含金属溶液以制备预选尺寸的金属溶胶颗粒，用偶联化合物涂覆颗粒，然后与至少一种选择的免疫化学反应性组分结合。 具有不同免疫化学特异性的颗粒也混合以产生具有多种选择的免疫化学特异性的试剂。

公开(公告)号：US5422277A

公开(公告)日：1995-06-06

申请号：US859212

申请日：1992-03-27

申请人： Mark C. Connelly ,  Utpal R. Chakraborty ,  Houston G. Brooks, Jr.

发明人： Mark C. Connelly ,  Utpal R. Chakraborty ,  Houston G. Brooks, Jr.

IPC分类号： C12Q1/00 ,  G01N1/30 ,  G01N15/14 ,  G01N33/48 ,  G01N33/533 ,  G01N33/545 ,  G01N33/569 ,  G01N31/00 ,  C12Q1/02 ,  G01N1/00

CPC分类号： G01N33/56988 ,  G01N1/30 ,  G01N33/56972 ,  G01N33/56983 ,  G01N2001/305 ,  Y10S435/975 ,  Y10S436/805 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25

摘要： A cell fixative composition for fixing the internal components of a cell without disrupting the cell surface components is disclosed. The composition contains a compound for preserving intracellular morphology, a compound for fixing internal components of the cell, a compound which facilitates transportation of components across the cellular membrane, and a compound for increasing permeability of cell membranes. Methods for analyzing cells which have been fixed using the composition are also disclosed.

摘要翻译： 公开了用于固定电池的内部组分而不破坏电池表面组分的电池固定组合物。 该组合物含有用于保留细胞内形态的化合物，用于固定细胞内部组分的化合物，促进组分跨细胞膜运输的化合物和用于增加细胞膜渗透性的化合物。 还公开了使用该组合物固定的细胞分析方法。

公开(公告)号：US5514602A

公开(公告)日：1996-05-07

申请号：US22544

申请日：1993-02-25

申请人： Houston G. Brooks, Jr. ,  Chi-Deu Chang ,  Utpal R. Chakraborty ,  Henry A. Graham, Jr. ,  Lloyd L. Hollenbeck, Jr. ,  Sharon R. Lawler ,  Jennifer Nasser ,  Ernest G. Schutt ,  Albert Venturini

发明人： Houston G. Brooks, Jr. ,  Chi-Deu Chang ,  Utpal R. Chakraborty ,  Henry A. Graham, Jr. ,  Lloyd L. Hollenbeck, Jr. ,  Sharon R. Lawler ,  Jennifer Nasser ,  Ernest G. Schutt ,  Albert Venturini

IPC分类号： B01J13/00 ,  G01N33/543 ,  G01N33/558 ,  G01N33/58 ,  G01N33/74 ,  G01N33/553

CPC分类号： G01N33/587 ,  B01J13/0004 ,  B01J13/0043 ,  G01N33/54326 ,  G01N33/54386 ,  G01N33/558 ,  G01N33/74 ,  Y10S435/975

摘要： A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.

摘要翻译： 用于各种免疫测定系统的方法和测试试剂盒。 提供一种制备含有预选尺寸的金属溶胶颗粒的金属溶胶试剂的方法。 在优化的pH条件下还原含金属溶液以制备预选尺寸的金属溶胶颗粒，用偶联化合物涂覆颗粒，然后与至少一种选择的免疫化学反应性组分结合。 具有不同免疫化学特异性的颗粒也混合以产生具有多种选择的免疫化学特异性的试剂。