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    METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS
    1.
    发明申请
    METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS 审中-公开
    分离蛋白质的方法，蛋白质和液体蛋白质染色剂的染色方法以及用于这些方法的蛋白质染色试剂盒

    公开(公告)号：US20090223823A1

    公开(公告)日：2009-09-10

    申请号：US11720927

    申请日：2005-12-06

    申请人： Ichiji Namatame ,  Yoshinori Ishii ,  Yoshimasa Saito ,  Takayoshi Komatsu ,  Yasuhiro Ogawa ,  Takashi Shibata ,  Hideki Kinoshita ,  Kenji Yokoyama

    发明人： Ichiji Namatame ,  Yoshinori Ishii ,  Yoshimasa Saito ,  Takayoshi Komatsu ,  Yasuhiro Ogawa ,  Takashi Shibata ,  Hideki Kinoshita ,  Kenji Yokoyama

    IPC分类号： B01D57/02 ,  C07K2/00 ,  G01N33/00

    CPC分类号： G01N27/44726

    摘要： A method of separating a protein by gel electrophoresis, which comprises a staining step for bringing a protein-containing sample into contact with a staining liquid containing a staining agent, a surfactant and a buffer solution, and an electrophoresis step for subjecting the protein-containing sample after the staining step to gel electrophoresis.According to the present invention, using a staining liquid containing a surfactant such as SDS and the like, a protein-containing sample can be stained in a short time, and can develop color with high sensitivity. In addition, since an excess staining agent migrates earlier than protein by electrophoresis, a washing operation is not necessary. Furthermore, by staining, after the first dimension electrophoresis, with a staining liquid containing a surfactant such as SDS and the like, the second dimension electrophoresis can be performed immediately. As a result, the number of steps can be reduced as compared to conventional protein separation methods, and the separation operation can be simplified. Hence, a method capable of staining and separating a protein conveniently and quickly by electrophoresis can be provided.

    摘要翻译： 一种通过凝胶电泳分离蛋白质的方法，其包括使含蛋白质的样品与含有染色剂，表面活性剂和缓冲溶液的染色液体接触的染色步骤，以及将含蛋白质的样品 染色步骤后进行凝胶电泳。 根据本发明，使用含有SDS等表面活性剂的染色液，可以在短时间内对含蛋白质的样品进行染色，可以以高灵敏度显色。 此外，由于过量的染色剂通过电泳比蛋白质迁移更早，所以不需要洗涤操作。 此外，通过染色，在第一维电泳之后，用含有诸如SDS等的表面活性剂的染色液进行染色，可以立即进行第二维电泳。 结果，与常规蛋白质分离方法相比，可以减少步骤数，并且可以简化分离操作。 因此，可以提供通过电泳方便快速地染色和分离蛋白质的方法。

    Electrophoresis apparatus and electrophoresis method
    7.
    发明授权
    Electrophoresis apparatus and electrophoresis method 有权
    电泳仪和电泳法

    公开(公告)号：US08500981B2

    公开(公告)日：2013-08-06

    申请号：US13054271

    申请日：2009-07-15

    申请人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

    发明人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

    IPC分类号： G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44773

    摘要： An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.

    摘要翻译： 电泳装置包括样品分离部分，用于容纳用于在水平方向上分离样品的样品分离介质，样品分离部分包含样品分离介质，使得样品分离介质具有暴露部分，至少一端 所述样品分离介质的表面与所述水平方向平行; 以及介质连接装置，用于在连接区域连接包含样品的介质到样品分离介质，所述样品包含含有样品的介质和满足特定等式的连接区域。 由此，可以提供比现有技术更好的精度的电泳技术。

    Sample separation/adsorption appliance
    8.
    发明授权
    Sample separation/adsorption appliance 有权
    样品分离/吸附装置

    公开(公告)号：US08449748B2

    公开(公告)日：2013-05-28

    申请号：US13001274

    申请日：2009-06-01

    申请人： Uichi Midorikawa ,  Takateru Matsunaga ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama

    发明人： Uichi Midorikawa ,  Takateru Matsunaga ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama

    IPC分类号： G01N27/447

    CPC分类号： G01N27/4473

    摘要： A sample separation/adsorption appliance (100) according to one embodiment of the present invention includes: a first buffer solution tank (103) equipped with a first electrode (101) and used for holding a first buffer solution; a second buffer solution tank (104) equipped with a second electrode (102) and used for holding a second buffer solution; a sample separation section (110) that holds a sample separation medium (131) for separating a sample; and a conductive section (120) that holds a conductive medium (133), wherein: the sample separation medium (131) and the conductive medium (133) are in contact with the sample adsorption member (132), respectively, on the opposite sides thereof; an end of the sample separation medium (131) opposite to an end being in contact with the sample adsorption member (132) is connected to an inside of the first buffer solution tank (103); and an end of the conductive medium (133) opposite to an end being in contact with the sample adsorption member (132) is connected to an inside of the second buffer solution tank (104). With this arrangement, a sample separation/adsorption appliance that enables efficient transfer of a sample is offered.

    摘要翻译： 根据本发明的一个实施方案的样品分离/吸附装置（100）包括：装备有第一电极（101）并用于保持第一缓冲溶液的第一缓冲溶液罐（103） 装备有第二电极（102）并用于保持第二缓冲溶液的第二缓冲溶液罐（104）; 样品分离部（110），其保持用于分离样品的样品分离介质（131） 以及保持导电介质（133）的导电部（120），其中：所述样品分离介质（131）和所述导电介质（133）分别在所述样品吸附部件（132）的相对侧 的; 与第一缓冲溶液罐（103）的内部连接的是与样品吸附部件（132）接触的端部相反的样品分离介质（131）的端部。 并且与与样品吸附构件（132）接触的端部相对的导电介质（133）的端部连接到第二缓冲溶液罐（104）的内部。 通过这种布置，提供了能够有效地转移样品的样品分离/吸附装置。

    SAMPLE SEPARATION/ADSORPTION APPLIANCE
    9.
    发明申请
    SAMPLE SEPARATION/ADSORPTION APPLIANCE 有权
    样品分离/吸附装置

    公开(公告)号：US20110094887A1

    公开(公告)日：2011-04-28

    申请号：US13001274

    申请日：2009-06-01

    申请人： Uichi Midorikawa ,  Takateru Matsunaga ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama

    发明人： Uichi Midorikawa ,  Takateru Matsunaga ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama

    IPC分类号： G01N27/447

    CPC分类号： G01N27/4473

    摘要： A sample separation/adsorption appliance (100) according to one embodiment of the present invention includes: a first buffer solution tank (103) equipped with a first electrode (101) and used for holding a first buffer solution; a second buffer solution tank (104) equipped with a second electrode (102) and used for holding a second buffer solution; a sample separation section (110) that holds a sample separation medium (131) for separating a sample; and a conductive section (120) that holds a conductive medium (133), wherein: the sample separation medium (131) and the conductive medium (133) are in contact with the sample adsorption member (132), respectively, on the opposite sides thereof; an end of the sample separation medium (131) opposite to an end being in contact with the sample adsorption member (132) is connected to an inside of the first buffer solution tank (103); and an end of the conductive medium (133) opposite to an end being in contact with the sample adsorption member (132) is connected to an inside of the second buffer solution tank (104). With this arrangement, a sample separation/adsorption appliance that enables efficient transfer of a sample is offered.

    摘要翻译： 根据本发明的一个实施方案的样品分离/吸附装置（100）包括：装备有第一电极（101）并用于保持第一缓冲溶液的第一缓冲溶液罐（103） 装备有第二电极（102）并用于保持第二缓冲溶液的第二缓冲溶液罐（104）; 样品分离部（110），其保持用于分离样品的样品分离介质（131） 以及保持导电介质（133）的导电部（120），其中：所述样品分离介质（131）和所述导电介质（133）分别在所述样品吸附部件（132）的相对侧 的; 与第一缓冲溶液罐（103）的内部连接的是与样品吸附部件（132）接触的端部相反的样品分离介质（131）的端部。 并且与与样品吸附构件（132）接触的端部相对的导电介质（133）的端部连接到第二缓冲溶液罐（104）的内部。 通过这种布置，提供了能够有效地转移样品的样品分离/吸附装置。