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    L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from gluconobacter oxydans T-100
    1.
    发明授权
    L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from gluconobacter oxydans T-100 失效
    L-山梨糖脱氢酶和由氧化葡萄糖葡萄球菌T-100获得的新型L-山梨糖酮脱氢酶

    公开(公告)号：US06197562B1

    公开(公告)日：2001-03-06

    申请号：US09118317

    申请日：1998-07-17

    申请人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Suzuki

    发明人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Suzuki

    IPC分类号： C12N904

    CPC分类号： C12N9/0006 ,  Y10S435/822

    摘要： A novel L-sorbose dehydrogenase (SDH) and a novel L-sorbosone dehydrogenase both derived from Gluconobacter oxydans T-100, a DNA which encodes the SDH and/or SNDH, an expression vector which contains the DNA, a host cell transformed by the expression vector and a process for producing the SDH and/or SNDH, which comprises culturing the host cell in a medium and recovering the SDH and/or SNDH from the resulting culture. The SDH and SNDH of the present invention are useful enzymes having preferable properties for the production of 2-keto-L-gulonic acid, as well as L-ascorbic acid. According to the production method of the present invention, the SDH and SNDH having such preferable properties can be produced in large amounts by genetic engineering.

    摘要翻译： 一种新型的L-山梨糖脱氢酶（SDH）和一种新型的L-山梨糖酮脱氢酶，它们都衍生自氧化葡糖杆菌T-100，一种编码SDH和/或SNDH的DNA，含有该DNA的表达载体，由 表达载体和产生SDH和/或SNDH的方法，其包括在培养基中培养宿主细胞并从所得培养物中回收SDH和/或SNDH。 本发明的SDH和SNDH是生产2-酮-L-古洛糖酸以及L-抗坏血酸的优选性质的有用的酶。 根据本发明的制造方法，可以通过遗传工程大量生产具有优选性能的SDH和SNDH。

    METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS
    4.
    发明申请
    METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS 审中-公开
    分离蛋白质的方法，蛋白质和液体蛋白质染色剂的染色方法以及用于这些方法的蛋白质染色试剂盒

    公开(公告)号：US20090223823A1

    公开(公告)日：2009-09-10

    申请号：US11720927

    申请日：2005-12-06

    申请人： Ichiji Namatame ,  Yoshinori Ishii ,  Yoshimasa Saito ,  Takayoshi Komatsu ,  Yasuhiro Ogawa ,  Takashi Shibata ,  Hideki Kinoshita ,  Kenji Yokoyama

    发明人： Ichiji Namatame ,  Yoshinori Ishii ,  Yoshimasa Saito ,  Takayoshi Komatsu ,  Yasuhiro Ogawa ,  Takashi Shibata ,  Hideki Kinoshita ,  Kenji Yokoyama

    IPC分类号： B01D57/02 ,  C07K2/00 ,  G01N33/00

    CPC分类号： G01N27/44726

    摘要： A method of separating a protein by gel electrophoresis, which comprises a staining step for bringing a protein-containing sample into contact with a staining liquid containing a staining agent, a surfactant and a buffer solution, and an electrophoresis step for subjecting the protein-containing sample after the staining step to gel electrophoresis.According to the present invention, using a staining liquid containing a surfactant such as SDS and the like, a protein-containing sample can be stained in a short time, and can develop color with high sensitivity. In addition, since an excess staining agent migrates earlier than protein by electrophoresis, a washing operation is not necessary. Furthermore, by staining, after the first dimension electrophoresis, with a staining liquid containing a surfactant such as SDS and the like, the second dimension electrophoresis can be performed immediately. As a result, the number of steps can be reduced as compared to conventional protein separation methods, and the separation operation can be simplified. Hence, a method capable of staining and separating a protein conveniently and quickly by electrophoresis can be provided.

    摘要翻译： 一种通过凝胶电泳分离蛋白质的方法，其包括使含蛋白质的样品与含有染色剂，表面活性剂和缓冲溶液的染色液体接触的染色步骤，以及将含蛋白质的样品 染色步骤后进行凝胶电泳。 根据本发明，使用含有SDS等表面活性剂的染色液，可以在短时间内对含蛋白质的样品进行染色，可以以高灵敏度显色。 此外，由于过量的染色剂通过电泳比蛋白质迁移更早，所以不需要洗涤操作。 此外，通过染色，在第一维电泳之后，用含有诸如SDS等的表面活性剂的染色液进行染色，可以立即进行第二维电泳。 结果，与常规蛋白质分离方法相比，可以减少步骤数，并且可以简化分离操作。 因此，可以提供通过电泳方便快速地染色和分离蛋白质的方法。

    Vector derivatives of gluconobacter plasmid pF4
    7.
    发明授权
    Vector derivatives of gluconobacter plasmid pF4 失效
    葡萄糖杆菌质粒pF4的载体衍生物

    公开(公告)号：US06399340B1

    公开(公告)日：2002-06-04

    申请号：US09509565

    申请日：2000-06-23

    申请人： Yoshimasa Saito ,  Yuji Noguchi ,  Koji Yoshikawa ,  Shinsuke Soeda

    发明人： Yoshimasa Saito ,  Yuji Noguchi ,  Koji Yoshikawa ,  Shinsuke Soeda

    IPC分类号： C12P760

    CPC分类号： C12N9/0006 ,  C12N15/74 ,  C12P7/60

    摘要： A DNA derived from plasmid pF4, which contains a region involved in the control of autonomous replication in a bacterial cell belonging to the genus Gluconobactor, with or without a part of the polynucleotide region not essential for the autonomous replication therein, particularly, the same DNA comprising, as the region involved in the control of autonomous replication in the bacterial cell belonging to the genus Gluconobactor, a part or the entirety of the polynucleotide of nucleotide No. 2897-3969 region of Sequence Listing SEQ:ID No. 1. A plasmid vector containing this DNA, transformant transformed with this plasmid vector, and a method for producing a physiologically active substance comprising culturing this transformant. The full length nucleotide sequence of plasmid pF4 and the region containing DNA involved in the control of automonous replication in the bacterial cell belonging to the genus Gluconobactor were specified, whereby the region not essential for the automonous replication could be removed to provide a shortened pF4. The shortening of the region in the vector for autonomous replication increases the remaining region of the vector and allows incorporation of many structural genes into the vector. Consequently, the vector can have many functions.

    摘要翻译： 来自质粒pF4的DNA，其含有涉及控制属于Gluconobactor属的细菌细胞中的自主复制的区域，具有或不具有对其自主复制不是必需的一部分多核苷酸区域，特别是相同的DNA 包括作为控制属于Gluconobactor属的细菌细胞中的自主复制的区域的部分或全部是序列表SEQ ID NO：1的核苷酸2897-3969区域的多核苷酸的部分或全部。质粒 含有该DNA的载体，用该质粒载体转化的转化体，以及生产生物活性物质的方法，包括培养该转化体。 规定了质粒pF4的全长核苷酸序列和涉及控制属于Gluconobactor属细菌细胞中的自动复制控制的DNA的区域，由此可以除去对于自动复制不是必需的区域以提供缩短的pF4。 自由复制载体中区域的缩短增加了载体的剩余区域，并允许将许多结构基因并入载体中。 因此，矢量可以有很多功能。

    L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from Gluconobacter oxydans T-100
    8.
    发明授权
    L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from Gluconobacter oxydans T-100 失效
    L-山梨糖脱氢酶和由氧化葡糖杆菌T-100获得的新型L-山梨糖酮脱氢酶

    公开(公告)号：US5861292A

    公开(公告)日：1999-01-19

    申请号：US942673

    申请日：1997-10-02

    申请人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Suzuki

    发明人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Suzuki

    IPC分类号： C12N9/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/0006 ,  Y10S435/822

    摘要： A novel L-sorbose dehydrogenase (SDH) and a novel L-sorbosone dehydrogenase (SNDH) both derived from Gluconobacter oxydans T-100, a DNA which encodes the SDH and/or SNDH, an expression vector which contains the DNA, a host cell transformed by the expression vector and a process for producing the SDH and/or SNDH, which comprises culturing the host cell in a medium and recovering the SDH and/or SNDH from the resulting culture. The SDH and SNDH of the present invention are useful enzymes having preferable properties for the production of 2-keto-L-gulonic acid, as well as L-ascorbic acid. According to the production method of the present invention, the SDH and SNDH having such preferable properties can be produced in large amounts by genetic engineering.

    摘要翻译： 来自氧化葡糖杆菌T-100的新型L-山梨糖脱氢酶（SDH）和新型L-山梨糖酮脱氢酶（SNDH），编码SDH和/或SNDH的DNA，含有DNA的表达载体，宿主细胞 通过表达载体和产生SDH和/或SNDH的方法转化，其包括在培养基中培养宿主细胞并从所得培养物中回收SDH和/或SNDH。 本发明的SDH和SNDH是生产2-酮-L-古洛糖酸以及L-抗坏血酸的优选性质的有用的酶。 根据本发明的制造方法，可以通过遗传工程大量生产具有优选性能的SDH和SNDH。

    Cephalosporin C acylase
    9.
    发明授权
    Cephalosporin C acylase 失效
    头孢菌素C酰基转移酶

    公开(公告)号：US5804429A

    公开(公告)日：1998-09-08

    申请号：US633760

    申请日：1996-05-01

    申请人： Mineo Niwa ,  Yoshimasa Saito ,  Takao Fujimura ,  Yoshinori Ishii ,  Yuji Noguchi

    发明人： Mineo Niwa ,  Yoshimasa Saito ,  Takao Fujimura ,  Yoshinori Ishii ,  Yuji Noguchi

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/14 ,  C12N9/80 ,  C12N15/55 ,  C12P35/02 ,  C12R1/19 ,  C07H21/04 ,  C12N15/00 ,  C12P21/06

    CPC分类号： C12P35/02 ,  C12N9/80

    摘要： A mutant CC acylase wherein at least one amino acid at the Ala.sup.49, Met.sup.164, Ser.sup.166, Met.sup.174, Glu.sup.358, Met.sup.465, Met.sup.506, or Met.sup.750 position of the amino acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like.

    摘要翻译： PCT No.PCT / JP94 / 01799 Sec。 371日期：1996年5月1日 102（e）日期1996年5月1日PCT 1994年10月26日PCT公布。 公开号WO95 / 12680 日期1995年5月11日，其中天然CC酰基转移酶的氨基酸序列的Ala49，Met164，Ser166，Met174，Glu358，Met465，Met506或Met750位置的至少一个氨基酸被不同的氨基酸替代的突变CC酰基转移酶 编码该DNA的DNA，含有上述DNA的表达载体，用上述表达载体转化的微生物，通过培养上述转化体产生CC酰基转移酶及其制备化合物的用途。 本发明的突变CC酰基转移酶在酶效力，pH曲线的改变，加工效率等方面具有期望的性质。

    Optical path rotating device used with linear array laser diode and laser apparatus applied therewith
    10.
    发明授权
    Optical path rotating device used with linear array laser diode and laser apparatus applied therewith 失效
    用于线性阵列激光二极管的光路旋转装置及其应用的激光装置

    公开(公告)号：US5513201A

    公开(公告)日：1996-04-30

    申请号：US235455

    申请日：1994-04-28

    申请人： Satoshi Yamaguchi ,  Masahiro Daimon ,  Koichi Chiba ,  Tetsurou Kobayashi ,  Yoshimasa Saito

    发明人： Satoshi Yamaguchi ,  Masahiro Daimon ,  Koichi Chiba ,  Tetsurou Kobayashi ,  Yoshimasa Saito

    IPC分类号： G02B6/42 ,  G02B27/09 ,  H01S3/00 ,  H01S5/00 ,  H01S5/40 ,  H01S3/094

    CPC分类号： G02B19/0057 ,  G02B19/0028 ,  G02B27/0944 ,  G02B27/0961 ,  G02B27/0966 ,  G02B27/0972 ,  G02B6/425 ,  H01S5/4025 ,  G02B6/4206 ,  H01S3/005 ,  H01S5/005

    摘要： An optical path rotating device, disposed in front of a linear array laser diode having a plurality of long and narrow and linearly arranged emitters for emitting a group of laser beams in the form of a dotted line, the optical path rotating device being able to convert the laser beams from the emitters into laser beams lined up in the form of ladder rungs by receiving the group of laser beams collimated by being refracted in a direction substantially perpendicular to the direction of the dotted line, rotating the positions of the laser beams from the emitters substantially for a right angle, and emitting the laser beams, and a laser apparatus, which uses the optical path rotating device, for collimating the substantially rung-shaped laser beams into two directions independently, bringing the laser means into focus, thus increasing the density of the laser energy at the focus.

    摘要翻译： 一种光路旋转装置，设置在线阵列激光二极管的前面，该线阵列激光二极管具有多个长而窄的线性排列的发射体，用于发出虚线形式的一组激光束，该光路旋转装置能够转换 通过接收通过在与虚线的方向基本垂直的方向上被折射而准直的激光束组，将来自发射器的激光束形成为阶梯形式的激光束，将激光束的位置从 发射器基本上成直角，并且发射激光束;以及激光装置，其使用光路旋转装置，用于将大致梯形激光束独立地准直到两个方向，使激光装置聚焦，从而增加 焦点处的激光能量的密度。