公开(公告)号：US08604278B2

公开(公告)日：2013-12-10

申请号：US13113615

申请日：2011-05-23

申请人： Ute Heim ,  Helke Hillebrand ,  Irene Kunze ,  Karin Herbers ,  Uwe Sonnewald ,  Eric Glickmann ,  Wolfgang Lein ,  Rüdiger Hell ,  Ricarda Jost

发明人： Ute Heim ,  Helke Hillebrand ,  Irene Kunze ,  Karin Herbers ,  Uwe Sonnewald ,  Eric Glickmann ,  Wolfgang Lein ,  Rüdiger Hell ,  Ricarda Jost

IPC分类号： A01H5/00 ,  C12N15/63 ,  C12N15/82 ,  C12N15/87 ,  C12N5/14 ,  C07H21/00 ,  C07H21/04

CPC分类号： C12N15/8216

摘要： The invention relates to expression cassettes and vectors which contain constitutive promoters of plants and to the use of these expression cassettes or vectors for transgenic expression of nucleic acid sequences, preferably selection markers, in organisms, preferably in plants. The invention further relates to transgenic plants which have been transformed with the expression cassettes or vectors, to cultures, parts or propagation material derived from these plants, and to the use of these plants for the production of food and animal feedstuffs, seed, pharmaceuticals, or fine chemicals.

摘要翻译： 本发明涉及含有植物的组成型启动子的表达盒和载体，以及这些表达盒或载体用于在生物体，优选植物中的核酸序列，优选选择标记的转基因表达的用途。 本发明还涉及已经用表达盒或载体转化的转基因植物，衍生自这些植物的培养物，部分或繁殖材料，以及这些植物用于生产食品和动物饲料，种子，药物， 或精细化学品。

公开(公告)号：US08022272B2

公开(公告)日：2011-09-20

申请号：US10755677

申请日：2004-01-13

申请人： Ute Heim ,  Helke Hillebrand ,  Irene Kunze ,  Karin Herbers ,  Uwe Sonnewald ,  Eric Glickmann ,  Wolfgang Lein ,  Rüdiger Hell ,  Ricarda Jost

发明人： Ute Heim ,  Helke Hillebrand ,  Irene Kunze ,  Karin Herbers ,  Uwe Sonnewald ,  Eric Glickmann ,  Wolfgang Lein ,  Rüdiger Hell ,  Ricarda Jost

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/87 ,  C12N5/14 ,  C12N15/63 ,  C07H21/04 ,  C07H21/00

CPC分类号： C12N15/8216

摘要： The invention relates to expression cassettes and vectors, which contain vegetable constitutive promoters and to the use of these expression cassettes or vectors for the transgenic expression of nucleic acid sequences preferably selection markers in organisms, preferably in plants. The invention also relates to transgenic plants that have been transformed using these expression cassettes or vectors, to cultures, parts or propagation products derived from these plants, and to the use of these plants for producing food and animal feed agents, seeds, pharmaceuticals, or fine chemicals.

摘要翻译： 本发明涉及含有植物组成型启动子的表达盒和载体，以及这些表达盒或载体用于转基因表达核酸序列的优点，优选在生物体中，优选在植物中的选择标记。 本发明还涉及已经使用这些表达盒或载体转化的转基因植物，衍生自这些植物的培养物，部分或繁殖产物，以及这些植物用于生产食物和动物饲料，种子，药物或 精细化学品。

公开(公告)号：US20110289622A1

公开(公告)日：2011-11-24

申请号：US13113615

申请日：2011-05-23

申请人： Ute Heim ,  Helke Hillebrand ,  Irene Kunze ,  Karin Herbers ,  Uwe Sonnewald ,  Eric Glickmann ,  Wolfgang Lein ,  Rüdiger Hell ,  Ricarda Jost

发明人： Ute Heim ,  Helke Hillebrand ,  Irene Kunze ,  Karin Herbers ,  Uwe Sonnewald ,  Eric Glickmann ,  Wolfgang Lein ,  Rüdiger Hell ,  Ricarda Jost

IPC分类号： A01H1/00 ,  C12N9/00 ,  C12N5/10 ,  C12N1/21 ,  A01H5/00 ,  C12N1/19 ,  C12N15/63 ,  C07K14/00 ,  C07K16/00 ,  A01K67/027 ,  C12Q1/68 ,  C12N1/15

CPC分类号： C12N15/8216

摘要： The invention relates to expression cassettes and vectors which contain constitutive promoters of plants and to the use of these expression cassettes or vectors for transgenic expression of nucleic acid sequences, preferably selection markers, in organisms, preferably in plants. The invention further relates to transgenic plants which have been transformed with the expression cassettes or vectors, to cultures, parts or propagation material derived from these plants, and to the use of these plants for the production of food and animal feedstuffs, seed, pharmaceuticals, or fine chemicals.

摘要翻译： 本发明涉及含有植物的组成型启动子的表达盒和载体，以及这些表达盒或载体用于在生物体，优选植物中的核酸序列，优选选择标记的转基因表达的用途。 本发明还涉及已经用表达盒或载体转化的转基因植物，衍生自这些植物的培养物，部分或繁殖材料，以及这些植物用于生产食品和动物饲料，种子，药物， 或精细化学品。

公开(公告)号：US20050216967A1

公开(公告)日：2005-09-29

申请号：US10755677

申请日：2004-01-13

申请人： Ute Heim ,  Helke Hillebrand ,  Irene Kunze ,  Karin Herbers ,  Uwe Sonnewald ,  Eric Glickmann ,  Wolfgang Lein ,  Rudiger Hell ,  Ricarda Jost

发明人： Ute Heim ,  Helke Hillebrand ,  Irene Kunze ,  Karin Herbers ,  Uwe Sonnewald ,  Eric Glickmann ,  Wolfgang Lein ,  Rudiger Hell ,  Ricarda Jost

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8216

摘要： The invention relates to expression cassettes and vectors which contain constitutive promoters of plants and to the use of said expression cassettes or vectors for transgenic expression of nucleic acid sequences, preferably selection markers, in organisms, preferably in plants. The invention further relates to transgenic plants which have been transformed with said expression cassettes or vectors, to cultures, parts or propagation material derived therefrom and also to the use of same for the production of food- and feedstuffs, seed, pharmaceuticals or fine chemicals.

摘要翻译： 本发明涉及含有植物组成型启动子的表达盒和载体，以及使用所述表达盒或载体用于在生物体，优选植物中的核酸序列，优选选择标记的转基因表达。 本发明还涉及已经用所述表达盒或载体转化的转基因植物，从其衍生的培养物，部分或繁殖材料，以及用于生产食品和饲料，种子，药物或精细化学品的转基因植物。

公开(公告)号：US20070113300A1

公开(公告)日：2007-05-17

申请号：US10581293

申请日：2004-11-30

申请人： Thomas Ehrhardt ,  Andreas Reindl ,  Annette Freund ,  Ralf-Michael Schmidt ,  Uwe Sonnewald ,  Marc Nigel ,  Wolfgang Lein ,  Frederik Bornke ,  Kirsten Deist

发明人： Thomas Ehrhardt ,  Andreas Reindl ,  Annette Freund ,  Ralf-Michael Schmidt ,  Uwe Sonnewald ,  Marc Nigel ,  Wolfgang Lein ,  Frederik Bornke ,  Kirsten Deist

IPC分类号： A01H1/00 ,  C12Q1/37 ,  C12P21/06 ,  A01N25/00 ,  C12N9/48 ,  C12N5/04 ,  C12N15/82 ,  C07H21/04

CPC分类号： C12N9/14 ,  C12N15/8261 ,  C12Q1/37 ,  G01N33/56961 ,  G01N33/573 ,  Y02A40/146

摘要： The present invention relates to Clp-protease, which, when absent, brings about reduced growth and chlorotic leaves as target for herbicides. For this purpose, novel nucleic acid sequences encompassing SEQ ID NO:3, SEQ ID NO:11 and SEQ ID NO: 17 and functional equivalents of SEQ ID NO:3, SEQ ID NO:11 and SEQ ID NO: 17 are provided. Moreover, the present invention relates to the use of Clp-protease in a method for identifying compounds with herbicidal or growth-regulatory activity, and to the use of the compounds identified by this method as herbicides or growth regulators.

摘要翻译： 本发明涉及Clp-蛋白酶，当不存在时，会产生减少的生长和褪绿叶作为除草剂的靶标。 为此，提供包含SEQ ID NO：3，SEQ ID NO：11和SEQ ID NO：17的新型核酸序列和SEQ ID NO：3，SEQ ID NO：11和SEQ ID NO：17的功能等同物。 此外，本发明涉及Clp-蛋白酶在鉴定具有除草或生长调节活性的化合物的方法中的用途，以及通过该方法鉴定的化合物作为除草剂或生长调节剂的用途。

公开(公告)号：US07662603B2

公开(公告)日：2010-02-16

申请号：US11949874

申请日：2007-12-04

申请人： Uwe Sonnewald ,  Frederik Boernke ,  Kirsten Deist ,  Marc Stitt Nigel ,  Wolfgang Lein ,  Thomas Ehrhardt ,  Andreas Reindl ,  Ralf-Michael Schmidt ,  Annette Freund

发明人： Uwe Sonnewald ,  Frederik Boernke ,  Kirsten Deist ,  Marc Stitt Nigel ,  Wolfgang Lein ,  Thomas Ehrhardt ,  Andreas Reindl ,  Ralf-Michael Schmidt ,  Annette Freund

IPC分类号： C12N9/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/1014 ,  C12N15/8274

摘要： The present invention relates to serine hydroxymethyltransferase (E.C. 2.1.2.1) as novel target for herbicides, and to nucleic acid sequences encoding a polypeptide with the biological activity of a serine hydroxymethyltransferase, which, when not present, bring about growth retardation symptoms and chlorotic leaves, comprising the nucleic acid sequence SEQ ID No:3, and functional equivalents of the abovementioned nucleic acid sequence or the nucleic acid sequence SEQ ID NO:7 and functional equivalents of the abovementioned nucleic acid sequence. Moreover, the present invention relates to the use of the abovementioned nucleic acid sequences, of functional analogs of the SEQ ID NO:3 or SEQ ID NO:7 or of polypeptides encoded by one of the abovementioned nucleic acid sequences in a method for identifying herbicidally active compounds which inhibit serine hydroxymethyltransferases, and to the use of these compounds which have been identified by the method as herbicides.

摘要翻译： 本发明涉及丝氨酸羟甲基转移酶（EC 2.1.2.1）作为除草剂的新靶标，以及编码具有丝氨酸羟甲基转移酶生物活性的多肽的核酸序列，当不存在时，其产生生长迟缓症状和褪绿叶 包括核酸序列SEQ ID No：3和上述核酸序列或核酸序列SEQ ID NO：7的功能等同物和上述核酸序列的功能等同物。 此外，本发明涉及上述核酸序列，SEQ ID NO：3或SEQ ID NO：7的功能类似物或由上述核酸序列之一编码的多肽在除草方法中的用途 抑制丝氨酸羟甲基转移酶的活性化合物，以及通过该方法鉴定的这些化合物作为除草剂的用途。

公开(公告)号：US07319013B2

公开(公告)日：2008-01-15

申请号：US10507989

申请日：2003-03-13

申请人： Uwe Sonnewald ,  Frederik Börnke ,  Kirsten Deist ,  Marc Stitt Nigel ,  Wolfgang Lein ,  Thomas Ehrhardt ,  Andreas Reindl ,  Ralf-Michael Schmidt ,  Annette Freund

发明人： Uwe Sonnewald ,  Frederik Börnke ,  Kirsten Deist ,  Marc Stitt Nigel ,  Wolfgang Lein ,  Thomas Ehrhardt ,  Andreas Reindl ,  Ralf-Michael Schmidt ,  Annette Freund

IPC分类号： C12Q1/48 ,  C12N9/10 ,  C12N5/04 ,  C12N5/14 ,  C07H21/04

CPC分类号： C12N9/1014 ,  C12N15/8274

摘要： The present invention relates to serine hydroxymethyltransferase (E.C. 2.1.2.1) as novel target for herbicides, and to nucleic acid sequences encoding a polypeptide with the biological activity of a serine hydroxymethyltransferase, which, when not present, bring about growth retardation symptoms and chlorotic leaves, comprising the nucleic acid sequence SEQ ID No:3, and functional equivalents of the abovementioned nucleic acid sequence or the nucleic acid sequence SEQ ID NO:7 and functional equivalents of the abovementioned nucleic acid sequence. Moreover, the present invention relates to the use of the abovementioned nucleic acid sequences, of functional analogs of the SEQ ID NO:3 or SEQ ID NO:7 or of polypeptides encoded by one of the abovementioned nucleic acid sequences in a method for identifying herbicidally active compounds which inhibit serine hydroxymethyltransferases, and to the use of these compounds which have been identified by the method as herbicides.

摘要翻译： 本发明涉及丝氨酸羟甲基转移酶（EC 2.1.2.1）作为除草剂的新靶标，以及编码具有丝氨酸羟甲基转移酶生物活性的多肽的核酸序列，当不存在时，其产生生长迟缓症状和褪绿叶 包括核酸序列SEQ ID No：3和上述核酸序列或核酸序列SEQ ID NO：7的功能等同物和上述核酸序列的功能等同物。 此外，本发明涉及上述核酸序列，SEQ ID NO：3或SEQ ID NO：7的功能类似物或由上述核酸序列之一编码的多肽在除草方法中的用途 抑制丝氨酸羟甲基转移酶的活性化合物，以及通过该方法鉴定的这些化合物作为除草剂的用途。

公开(公告)号：US20110113494A1

公开(公告)日：2011-05-12

申请号：US12645976

申请日：2009-12-23

申请人： Uwe Sonnewald ,  Frederik Börnke ,  Kirsten Deist ,  Marc Stitt Nigel ,  Wolfgang Lein ,  Thomas Ehrhardt ,  Andreas Reindl ,  Ralf-Michael Schmidt ,  Annette Freund

发明人： Uwe Sonnewald ,  Frederik Börnke ,  Kirsten Deist ,  Marc Stitt Nigel ,  Wolfgang Lein ,  Thomas Ehrhardt ,  Andreas Reindl ,  Ralf-Michael Schmidt ,  Annette Freund

IPC分类号： A61K49/00 ,  C40B30/04 ,  A01H5/00 ,  A01K67/00 ,  C12N1/19 ,  C12N1/15 ,  C12N1/21 ,  C12Q1/68 ,  G01N33/573 ,  C12Q1/02 ,  C40B30/06 ,  A01H1/06

CPC分类号： C12N9/1014 ,  C12N15/8274

摘要： The present invention relates to serine hydroxymethyltransferase (E.C. 2.1.2.1) as novel target for herbicides, and to nucleic acid sequences encoding a polypeptide with the biological activity of a serine hydroxymethyltransferase, which, when not present, bring about growth retardation symptoms and chlorotic leaves, comprising the nucleic acid sequence SEQ ID No:3, and functional equivalents of the abovementioned nucleic acid sequence or the nucleic acid sequence SEQ ID NO:7 and functional equivalents of the abovementioned nucleic acid sequence. Moreover, the present invention relates to the use of the abovementioned nucleic acid sequences, of functional analogs of the SEQ ID NO:3 or SEQ ID NO:7 or of polypeptides encoded by one of the abovementioned nucleic acid sequences in a method for identifying herbicidally active compounds which inhibit serine hydroxymethyltransferases, and to the use of these compounds which have been identified by the method as herbicides.

摘要翻译： 本发明涉及丝氨酸羟甲基转移酶（EC 2.1.2.1）作为除草剂的新靶标，以及编码具有丝氨酸羟甲基转移酶生物活性的多肽的核酸序列，当不存在时，其产生生长迟缓症状和褪绿叶 包括核酸序列SEQ ID No：3和上述核酸序列或核酸序列SEQ ID NO：7的功能等同物和上述核酸序列的功能等同物。 此外，本发明涉及上述核酸序列，SEQ ID NO：3或SEQ ID NO：7的功能类似物或由上述核酸序列之一编码的多肽在除草方法中的用途 抑制丝氨酸羟甲基转移酶的活性化合物，以及通过该方法鉴定的这些化合物作为除草剂的用途。

公开(公告)号：US20070042451A1

公开(公告)日：2007-02-22

申请号：US10578637

申请日：2004-10-27

申请人： Thomas Ehrhardt ,  Andreas Reindl ,  Annette Freund ,  Ralf-Michael Schmidt ,  Uwe Sonnewald ,  Marc Nigel ,  Wolfgang Lein ,  Frederik Bornke ,  Kirsten Deist

发明人： Thomas Ehrhardt ,  Andreas Reindl ,  Annette Freund ,  Ralf-Michael Schmidt ,  Uwe Sonnewald ,  Marc Nigel ,  Wolfgang Lein ,  Frederik Bornke ,  Kirsten Deist

IPC分类号： C12Q1/48 ,  A01N63/00

CPC分类号： C12N15/8274 ,  C12N9/00

摘要： The invention relates to the use of glycine decarboxylase complex, which is used as a target for herbicides in the absence of growth retardations and chlorotic leaves. Novel nucleic acid sequences comprising SEQ ID NO:1 and functional equivalents of SEQ ID NO:1 are thus disclosed. The invention also relates to the use of glycine decarboxylase complex and the functional equivalents thereof in a method for identifying compounds having a herbicidal or growth-regulatory effect, in addition to the use of said compounds identified via said method as herbicides or growth regulators.

摘要翻译： 本发明涉及甘氨酸脱羧酶复合物的使用，其在不存在生长迟缓和褪绿叶的情况下用作除草剂的靶标。 因此，公开了包含SEQ ID NO：1和SEQ ID NO：1的功能等同物的新型核酸序列。 本发明还涉及除用于通过所述方法鉴定的所述化合物作为除草剂或生长调节剂之外，甘氨酸脱羧酶复合物及其功能等同物用于鉴定具有除草或生长调节作用的化合物的方法中。

公开(公告)号：US20070003934A1

公开(公告)日：2007-01-04

申请号：US10581223

申请日：2004-11-30

申请人： Thomas Ehrhardt ,  Andreas Reindl ,  Annette Freund ,  Ralf-Michael Schmidt ,  Uwe Sonnewald ,  Marc Nigel ,  Wolfgang Lein ,  Frederik Bornke

发明人： Thomas Ehrhardt ,  Andreas Reindl ,  Annette Freund ,  Ralf-Michael Schmidt ,  Uwe Sonnewald ,  Marc Nigel ,  Wolfgang Lein ,  Frederik Bornke

IPC分类号： C12Q1/68 ,  C12Q1/48 ,  C12P19/34 ,  A01N25/00

CPC分类号： A01N61/00 ,  C12N9/1007 ,  C12N15/8274 ,  G01N2333/91011 ,  G01N2500/00

摘要： The present invention relates to the use of 2-methyl-6-solanylbenzoquinone methyltransferase which, when absent, brings about retarded growth and chlorotic leaves, as target for herbicides. In this context, there are provided novel nucleic acid sequences comprising SEQ ID NO:5 and SEQ ID NO:7 and corresponding functional equivalents. Moreover, the present invention relates to the use of 2-methyl-6-solanylbenzoquinone methyltransferase and its functional equivalents in a method for identifying herbicidal or growth-regulatory compounds, and to the use of the compounds identifed via the method as herbicides or growth regulators.

摘要翻译： 本发明涉及2-甲基-6-苯甲酰基苯并醌甲基转移酶的用途，当不存在时，会导致延迟生长和褪绿叶作为除草剂的靶标。 在本文中，提供了包含SEQ ID NO：5和SEQ ID NO：7的新型核酸序列和相应的功能等同物。 此外，本发明涉及2-甲基-6-苯基苯醌基甲基转移酶及其功能等同物在鉴定除草或生长调节化合物的方法中的用途，以及通过该方法鉴定的化合物作为除草剂或生长调节剂的用途 。