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公开(公告)号：US4325706A
公开(公告)日：1982-04-20
申请号：US178486
申请日：1980-08-15
CPC分类号： G01N33/5094 , G01N15/1459 , G01N2015/0073 , G01N2015/0076 , G01N2015/0084
摘要： A sample of whole blood is stained with an acridine orange reagent, and is analyzed rapidly, a cell at a time, in a flow cytometry system having a sample stream dimension in the range of expected red cell dimensions. Red florescence and forward scatter data is utilized first to discriminate a cell from noise, and then to distinguish platelets from reticulocytes and red cells. The red cell and reticulocyte data is subjected to a correction such as rotational coordinate shift, and the shifted data are, by means of statistical procedures, utilized to determine threshold criteria separating red cells from reticulocytes, and to enumerate the cells on that basis.
摘要翻译： 用吖啶橙试剂染色全血样品，并在流式细胞仪系统中快速分析细胞，流式细胞仪系统中样品流尺寸在预期的红细胞尺寸范围内。 首先利用红色荧光和前向散射数据来区分细胞与噪声，然后将血小板与网织红细胞和红细胞区分开来。 对红细胞和网状细胞数据进行诸如旋转坐标移位的校正，并且通过统计程序利用移位的数据来确定将红细胞与网织红细胞分离的阈值标准，并在此基础上枚举细胞。
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公开(公告)号：US4665553A
公开(公告)日：1987-05-12
申请号：US605732
申请日：1984-05-01
CPC分类号： G01N15/1456 , G01N2021/4704 , G01N21/59 , G01N2201/04
摘要： Samples are prepared and applied to a cuvette, and the cuvette is translated in a given direction. Laser light is coupled to the sample through a Bragg cell, which causes the beam to scan the sample transversely to the given direction. Select optical parameters are monitored, and when they occur, the scan is stopped or slowed for a more complete analysis of the illuminated cell.
摘要翻译： 制备样品并将其应用于比色皿，并将反应杯沿给定方向平移。 激光通过布拉格细胞耦合到样品，这使得光束横向于给定方向扫描样品。 选择光学参数进行监控，当它们发生时，扫描被停止或减慢，以便对照明单元进行更完整的分析。
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公开(公告)号：US4217780A
公开(公告)日：1980-08-19
申请号：US055877
申请日：1979-07-09
CPC分类号： G01N35/1004
摘要： A fluid sampling nozzle is spatially fixed, and adapted to be supplied by fluid to be sampled. A nearby actuating switch, manipulated by the operator, energizes a sequence of sampling, cleaning, and drying steps. In particular, once fluid is withdrawn, the sample is removed. A waste removal sink pivots to a point beneath the nozzle, and in a series of motions by a probe washer assembly, the nozzle is successively washed, inside and out. The apparatus is then returned to its initial position, ready for successive sampling operations.
摘要翻译： 流体采样喷嘴在空间上固定，并且适于由待采样的流体提供。 由操作员操纵的附近的致动开关激励一系列取样，清洁和干燥步骤。 特别地，一旦液体被取出，就移除样品。 废物清除槽枢转到喷嘴下方的一个点，并且在通过探针垫圈组件的一系列运动中，喷嘴被连续地洗涤，内部和外部。 然后将设备返回到其初始位置，准备进行连续采样操作。
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