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公开(公告)号：US09176121B2
公开(公告)日：2015-11-03
申请号：US11057095
申请日：2005-02-11
CPC分类号： G01N33/5094 , G01N2015/0073 , G01N2015/008 , G01N2015/0084 , G01N2015/144 , G01N2015/1486 , G01N2015/1497
摘要： A method of identifying the elements of a blood sample including placing an aliquot of blood on a transparent substrate such as a coverslip. The blood is allowed to stand and the cells to settle to form a layer or matrix. Inverted microscopy is used to identify the elements in the sample. Various forms of illumination may be used alone or in multiple combinations. The method improves the accuracy due to homogenous distribution of formed elements in the wet drop or aliquot, simplifies the method, lowers the cost of the test and results in a shortened analytical cycle time.
摘要翻译： 一种识别血液样本的元件的方法，包括将等分的血液放置在诸如盖玻片的透明基底上。 允许血液静置并使细胞沉降以形成层或基质。 倒置显微镜用于识别样品中的元素。 各种形式的照明可以单独使用或以多种组合使用。 该方法由于在湿滴或等分试样中形成的元素的均匀分布而提高了准确性，简化了该方法，降低了测试成本并导致了缩短的分析周期时间。
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2.发明授权Apparatus and method for in vivo analysis of red and white blood cell indices 失效用于红细胞和白细胞指数的体内分析的装置和方法
公开(公告)号：US4998533A
公开(公告)日：1991-03-12
申请号：US320896
申请日：1989-03-09
申请人： James W. Winkelman
发明人： James W. Winkelman
CPC分类号： A61B5/14535 , A61B5/14555 , A61B5/411 , G01N15/1463 , G01N2015/0073 , G01N2015/008 , G01N2015/1486
摘要： Apparatus and methods for in vivo determination of red and white blood cell characteristics from a flow of red and white blood cells in mucous membranes are provided wherein image capturing means are employed to optically isolate images from a flow of blood cells and transmit those images to an image receiving means for encoding into electronic signals. The images are also filtered through light filters and subsequently transmitted to a computer for evaluation and analysis. The results of this analysis are then made available for display either on a CRT or hard copy printer.
摘要翻译： 提供了用于从粘液中的红细胞和白细胞流中体内测定红细胞和白细胞特征的装置和方法，其中使用图像捕获装置将血液细胞的图像光学隔离并将这些图像传输到 图像接收装置，用于编码成电子信号。 图像也通过滤光片过滤，随后传输到计算机进行评估和分析。 然后，分析结果可以在CRT或硬拷贝打印机上显示。
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3.发明授权Method and apparatus for determining erythrocyte sedimentation rate and hematocrit 失效用于确定红细胞沉降率和血细胞比容的方法和装置公开(公告)号：US06506606B1
公开(公告)日：2003-01-14
申请号：US08471536
申请日：1995-06-06
IPC分类号： G01N3349
CPC分类号： G01N15/042 , G01N15/0211 , G01N15/05 , Y10T436/111666
摘要： A method and apparatus is disclosed for determining the erythrocyte sedimentation rate and hematocrit simultaneously with the centrifugation of whole blood. A centrifuge separates the whole blood into its erythrocytes and its fluid portion. A video camera measures the levels of whole blood, erythrocytes and the fluid portion of the blood and records the time of the formation of an interface between the erythrocytes and fluid portion. A monitor displays the results of the recording. Also disclosed are the method steps performed.
摘要翻译： 公开了用于在全血离心的同时确定红细胞沉降速率和血细胞比容的方法和装置。 离心机将全血分离成红细胞及其流体部分。 摄像机测量全血，红细胞和血液部分的水平，并记录在红细胞和流体部分之间形成界面的时间。 显示器显示录制结果。 还公开了所执行的方法步骤。
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公开(公告)号：US20080138852A1
公开(公告)日：2008-06-12
申请号：US11057095
申请日：2005-02-11
IPC分类号： G01N1/30
CPC分类号： G01N33/5094 , G01N2015/0073 , G01N2015/008 , G01N2015/0084 , G01N2015/144 , G01N2015/1486 , G01N2015/1497
摘要： A method of identifying the elements of a blood sample including placing an aliquot of blood on a transparent substrate such as a coverslip. The blood is allowed to stand and the cells to settle to form a layer or matrix. Inverted microscopy is used to identify the elements in the sample. Various forms of illumination may be used alone or in multiple combinations. The method improves the accuracy due to homogenous distribution of formed elements in the wet drop or aliquot, simplifies the method, lowers the cost of the test and results in a shortened analytical cycle time.
摘要翻译： 一种识别血液样本的元件的方法，包括将等分的血液放置在诸如盖玻片的透明基底上。 允许血液静置并使细胞沉降以形成层或基质。 倒置显微镜用于识别样品中的元素。 各种形式的照明可以单独使用或以多种组合使用。 该方法由于在湿滴或等分试样中形成的元素的均匀分布而提高了准确性，简化了该方法，降低了测试成本并导致了缩短的分析周期时间。
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5.发明授权Apparatus for separating plasma or serum from the red cells with a blood sample 失效用血液样品从红细胞分离血浆或血清的装置公开(公告)号：US06398705B1
公开(公告)日：2002-06-04
申请号：US09661131
申请日：2000-09-13
IPC分类号： B04B502
CPC分类号： B04B5/0414 , G01N33/491
摘要： The method of rapidly separating plasma or serum from red blood cells comprising the steps of mounting a pair of spaced apart tubes coaxially related to one another head-to-head with a sharp-ended hollow needle disposed generally along the tube axes and between them, arranging the tubes in a vertical position one above the other in a centrifuge with the tubes closed with self-sealing stoppers, providing a partial vacuum in the upper tube, rotating the tubes at high speed about an axis parallel to and spaced from the axis of the tubes and needle, after separation of the red blood cells from the plasma and serum causing the tubes while still being rotated to be moved relative to one another such as to cause the needle to enter both tubes through the stoppers, separating the tubes while still being rotated by the centrifuge whereby to remove the needle from both tubes.
摘要翻译： 从红细胞中快速分离血浆或血清的方法包括以下步骤：将一对间隔开的管以相对于彼此头对头的方式同轴地相连，其中所述尖头端中空针通常沿着管轴和它们之间布置， 将管子放置在离心机中的一个垂直位置上，其中管子用自密封塞子封闭，在上管中提供部分真空，使管子以高速度绕与轴线平行并与其间隔开的轴线 管和针之后，红细胞从等离子体和血清分离出来，导致管子同时仍然被旋转以相对于彼此移动，以使针头通过塞子进入两个管，同时分离管 由离心机旋转，从而从两个管中取出针头。
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6.发明授权Apparatus and method for separating plasma or serum from the red cells of a blood sample 失效用于从血液样品的红细胞中分离血浆或血清的装置和方法
公开(公告)号：US6132353A
公开(公告)日：2000-10-17
申请号：US897420
申请日：1997-07-21
CPC分类号： B04B5/0414 , G01N33/491
摘要： Red cells are separated from plasma or serum of whole blood by arranging an evacuated tube sealed by a self-sealing stopper above and preferably coaxial with a tube containing a blood sample and sealed by a self-sealing stopper. A hollow double ended needle is held by a fixture and is disposed between and coaxial with the tubes and the tubes are placed in the fixture in a vertical position in a centrifuge and rotated about and spaced from an axis parallel to the axis of the tubes and needle. After the red cells are separated from the serum and/or plasma the tubes are pushed toward one another so that the needle penetrates both stoppers and the serum and/or plasma is drawn into the upper tube. The tubes and needle are separated while still being rotated so that the whole blood components are separated as required.
摘要翻译： 将红细胞与全血的血浆或血清分开，通过将自封闭塞子密封的真空管布置在上面并且优选地与含有血液样品的管同轴并由自密封塞密封。 空心双头针由固定器固定并且设置在管之间并且与管同轴，并且管在离心机中处于垂直位置并且与平行于管的轴线的轴线旋转并且与其平行的轴线间隔开，并且 针。 在从血清和/或等离子体分离红细胞后，将管彼此推动，使得针穿透两个塞子，将血清和/或血浆拉入上管。 管和针在仍然旋转的同时分离，使得全部血液成分根据需要分离。
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