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公开(公告)号：US20090269822A1
公开(公告)日：2009-10-29
申请号：US12499362
申请日：2009-07-08
摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrile hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrile wherein the nitrile is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NCIMB 41164, a mutant thereof and a nitrile hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NCIMB 41164.
摘要翻译： 作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。 要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。 要求从微生物获得的腈水合酶。 还要求的是从相应的腈制备酰胺的方法，其中腈在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NCIMB 41164， 其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的腈水合酶或其突变体。 还要求保存红玫瑰色红球菌NCIMB 41164的方法。
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2.发明授权Strain-of Rhodococcus rhodochrous NCIMB 41164 and its use as producer of nitrile hydratase 有权菌株Rhodococcus rhodochrous NCIMB 41164及其用作腈水合酶的生产者公开(公告)号：US07575912B2
公开(公告)日：2009-08-18
申请号：US10580448
申请日：2004-11-22
摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrite hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrite wherein the nitrite is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NUMB 41164, a mutant thereof and a nitrite hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NUMB 41164.
摘要翻译： 作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。 要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。 要求从微生物获得的亚硝酸盐水合酶。 还要求的是从相应的亚硝酸盐制备酰胺的方法，其中亚硝酸盐在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NUMB 41164， 其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的亚硝酸盐水合酶或其突变体。 还要求保存红玫瑰色红球菌NUMB 41164的方法。
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公开(公告)号：US08153406B2
公开(公告)日：2012-04-10
申请号：US12499362
申请日：2009-07-08
摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrile hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrile wherein the nitrile is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NCIMB 41164, a mutant thereof and a nitrile hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NCIMB 41164.
摘要翻译： 作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。 要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。 要求从微生物获得的腈水合酶。 还要求的是从相应的腈制备酰胺的方法，其中腈在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NCIMB 41164， 其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的腈水合酶或其突变体。 还要求保存红玫瑰色红球菌NCIMB 41164的方法。
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公开(公告)号：US20070077633A1
公开(公告)日：2007-04-05
申请号：US10580446
申请日：2004-11-22
申请人： Yvonne Armitage , Jatinder Kullar
发明人： Yvonne Armitage , Jatinder Kullar
CPC分类号： C12P13/02 , Y10S435/822
摘要： A method of producing an amide from the corresponding nitrile comprising the following steps, i) providing a microorganism capable of producing a nitrile hydratase biocatalyst, ii) culturing the microorganism in a growth medium, iii) storing the microorganism, iv) contacting the nitrile by the microorganism in an aqueous medium and thereby converting the nitrile to the amide, wherein the microorganism is stored as none actively growing free cells in a storage medium that comprises water. The stored microorganism may be as whole microbial cells, this may be in the form of a cell paste recovered from a fermentation medium; an aqueous suspension of the microbial cells, prepared using a suitable suspending medium such as water, physiological saline solution, or a suitable buffer solution, such as phosphate or comprises the fermentation broth that contains components of the fermentation culture medium and products of microbial culture. The microorganism exhibits no significant loss of activity, for example if stored even for at least 2 days, especially 3 to 28 days.
摘要翻译： 一种从相应的腈生产酰胺的方法，包括以下步骤：i）提供能够产生腈水合酶生物催化剂的微生物，ii）在生长培养基中培养微生物，iii）储存微生物，iv）使腈接触 微生物在水性介质中，从而将腈转化为酰胺，其中微生物作为无活性生长的游离细胞储存在包含水的储存培养基中。 储存的微生物可以是整个微生物细胞，其可以是从发酵培养基中回收的细胞糊的形式; 使用合适的悬浮介质如水，生理盐水或适当的缓冲溶液（例如磷酸盐）制备的微生物细胞的水性悬浮液，或包含含有发酵培养基的组分和微生物培养产物的发酵液。 微生物显示出不显着的活性损失，例如如果储存至少2天，特别是3至28天。
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公开(公告)号：US20060247323A1
公开(公告)日：2006-11-02
申请号：US11475698
申请日：2006-06-27
CPC分类号： A01N25/22 , A01N53/00 , A01N25/10 , A01N25/04 , A01N2300/00
摘要： An emulsion comprising an organic discontinuous phase which is distributed throughout a continuous aqueous phase, wherein the organic phase comprises a light sensitive active ingredient, and the emulsion is stabilised by a water soluble stabilising material in the aqueous phase, wherein the water-soluble stabilising material is a water-soluble stabilising polymer which has a plurality of hydrophilic and hydrophobic groups and is selected from partially hydrolysed polyvinyl acetate and addition copolymers formed from (i) at least one ethylenically unsaturated carboxylic acid esters and (ii) at least one ethylenically unsaturated carboxylic acid or ethylenically unsaturated carboxylic acid anhydride, and wherein the organic phase further comprises, a) an organic solvent which is a liquid at 25° C. and/or b) an organic phase stabilising material comprises hydrophobic moieties and is a material which is more soluble in the organic phase than the aqueous phase. The composition is useful for protecting light sensitive active ingredients which would otherwise in neat form decompose on exposure to light, preferably sunlight. The invention is of particular value when the light sensitive active ingredient is a pesticide, herbicide or a veterinary treatment active. Preferably the light sensitive active ingredient is a light sensitive pyrethroids. The invention also contemplates a domestic pest control formulation comprising light sensitive pesticides.
摘要翻译： 一种乳液，其包含分布在整个连续水相中的有机不连续相，其中所述有机相包含光敏活性成分，并且所述乳液通过所述水相中的水溶性稳定材料稳定化，其中所述水溶性稳定材料 是一种水溶性稳定聚合物，其具有多个亲水和疏水基团，并且选自部分水解的聚乙酸乙烯酯和由（i）至少一种烯键式不饱和羧酸酯和（ii）至少一种烯属不饱和羧酸形成的加成共聚物 酸或烯属不饱和羧酸酐，并且其中有机相还包含a）在25℃下为液体的有机溶剂和/或b）有机相稳定材料包含疏水部分，并且是更多的材料 可溶于有机相，而不是水相。 该组合物可用于保护光敏活性成分，否则其将在曝光于光，优选阳光下分解。 当光敏性活性成分是农药，除草剂或兽医治疗活性物质时，本发明是特别有价值的。 优选地，光敏活性成分是光敏拟除虫菊酯。 本发明还考虑了包含光敏农药的国内有害生物防治制剂。
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公开(公告)号：US20080038788A1
公开(公告)日：2008-02-14
申请号：US11631993
申请日：2005-07-01
申请人： Dinesh Mistry , Jatinder Kullar
发明人： Dinesh Mistry , Jatinder Kullar
CPC分类号： C08F220/06 , C08F220/56 , C12P7/40 , C12P13/02
摘要： A process of preparing an ethylenically unsaturated amide or an ethylenically unsaturated carboxylic acid or salt thereof from the corresponding ethylenically unsaturated nitrile wherein the nitrile is subjected to a hydration or hydrolysis reaction in an aqueous medium in the presence of a biocatalyst, wherein the nitrite contains above 2 ppm acrolein and the amide or carboxylic acid or salt thereof contains less than 2 ppm acrolein. The process can be used to prepare high purity acrylamide or acrylic acid (salt) from low quality acrylonitrile containing high levels of acrolein. Suitable biocatalysts include microorganisms of the Rhodococcus genus.
摘要翻译： 从相应的烯键式不饱和腈制备烯属不饱和酰胺或烯属不饱和羧酸或其盐的方法，其中腈在生物催化剂存在下在含水介质中进行水合或水解反应，其中亚硝酸盐含有上述 2ppm丙烯醛，酰胺或羧酸或其盐含有小于2ppm的丙烯醛。 该方法可用于从含有高含量丙烯醛的低品质丙烯腈制备高纯度丙烯酰胺或丙烯酸（盐）。 合适的生物催化剂包括红球菌属的微生物。
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公开(公告)号：US20070077634A1
公开(公告)日：2007-04-05
申请号：US10580448
申请日：2004-11-22
摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrite hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrite wherein the nitrite is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NUMB 41164, a mutant thereof and a nitrite hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NUMB 41164.
摘要翻译： 作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。 要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。 要求从微生物获得的亚硝酸盐水合酶。 还要求的是从相应的亚硝酸盐制备酰胺的方法，其中亚硝酸盐在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NUMB 41164， 其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的亚硝酸盐水合酶或其突变体。 还要求保存红玫瑰色红球菌NUMB 41164的方法。
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