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    Fluorescent proteins and uses thereof
    7.
    发明授权
    Fluorescent proteins and uses thereof 有权
    荧光蛋白及其用途

    公开(公告)号：US08541558B2

    公开(公告)日：2013-09-24

    申请号：US11381495

    申请日：2006-05-03

    申请人： Jenny J. Yang

    发明人： Jenny J. Yang

    IPC分类号： C07H21/04 ,  G01N33/53 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C07K14/43595 ,  G01N33/533 ,  G01N33/582

    摘要： A fluorescent sensor and methods for producing and using the fluorescent sensor. Such fluorescent sensors have broad applicability in characterizing cells and organisms, in detecting or measuring various cellular parameters, and in detecting or measuring protein-protein/peptide interactions.

    摘要翻译： 一种荧光传感器及其生产和使用荧光传感器的方法。 这种荧光传感器在表征细胞和生物体，检测或测量各种细胞参数以及检测或测量蛋白质/蛋白质/肽相互作用方面具有广泛的应用。

    Analyte sensors
    9.
    发明授权
    Analyte sensors 有权
    分析物传感器

    公开(公告)号：US09151767B2

    公开(公告)日：2015-10-06

    申请号：US10914769

    申请日：2004-08-09

    申请人： Jenny J. Yang

    发明人： Jenny J. Yang

    IPC分类号： G01N31/00 ,  G01N33/53 ,  G01N33/84 ,  G01N33/542

    CPC分类号： G01N33/84 ,  G01N33/542

    摘要： Described herein are analyte sensors with a tailored analyte binding motif that binds an analyte and a host protein operatively linked to the analyte binding motif, in which the binding of the analyte to the analyte binding motif produces a detectable change and manipulation of the analyte binding motif manipulates the responsiveness of the sensor. Also described herein are methods for constructing an analyte sensor by constructing a tailored analyte binding motif capable of responding to an analyte and operatively inserting the analyte binding motif into a host protein. Included is a method for quantifying an analyte by introducing a nucleotide sequence for a protein having an analyte sensor with a tailored analyte binding motif that is able to produce a detectable change upon excitation, expressing the protein, providing excitement to the analyte sensor, and quantifying the detectable change. Nucleic acid sequences for an analyte sensor with a tailored analyte binding motif sequence for an analyte binding peptide that produces a detectable change upon excitation and a host sequence for a host protein, in which the tailored binding motif sequence and the host protein sequence are operatively linked, and manipulation of the analyte binding motif sequence manipulates the responsiveness of the analyte sensor are also included.

    摘要翻译： 本文描述了具有定制的分析物结合基序的分析物传感器，其结合分析物和可操作地连接到分析物结合基序的宿主蛋白，其中分析物与分析物结合基序的结合产生可检测的变化并且分析物结合基序的操作 操纵传感器的响应性。 本文还描述了通过构建能够响应于分析物并将分析物结合基序可操作地插入宿主蛋白的定制的分析物结合基序来构建分析物传感器的方法。 包括一种通过为具有分析物传感器的蛋白质的核苷酸序列引入定制的分析物结合基序来定量分析物的方法，其能够在激发时产生可检测的变化，表达蛋白质，向分析物传感器提供兴奋，并量化 可检测的变化。 用于分析物传感器的核酸序列，其具有用于分析物结合肽的定制分析物结合基序序列，其在激发时产生可检测的变化，并且宿主蛋白质的宿主序列，其中定制的结合基序序列和宿主蛋白质序列可操作地连接 并且还包括对分析物结合基序序列的操纵操纵分析物传感器的响应性。