公开(公告)号：US20060134604A1

公开(公告)日：2006-06-22

申请号：US11303548

申请日：2005-12-16

申请人： Mark Smith ,  Kenneth Palmer ,  Gregory Pogue ,  John Lindbo ,  Kathleen Hanley ,  David Mannion ,  Gershon Wolfe

发明人： Mark Smith ,  Kenneth Palmer ,  Gregory Pogue ,  John Lindbo ,  Kathleen Hanley ,  David Mannion ,  Gershon Wolfe

IPC分类号： C12Q1/00 ,  G01N33/50

CPC分类号： C07K5/06165 ,  A61K38/00 ,  B01D15/1885 ,  B01D15/3804 ,  C07K5/06139

摘要： A plurality of proteins of interest, or peptides of interest, or other genetically expressed materials, are screened and subsequently produced using any of a variety of expression systems. The plurality of proteins are extracted from a plurality of separate, processed green juices, each green juice containing one of the proteins of interest. A multi-channel apparatus processes the various green juices, one green juice per channel. The apparatus is computer controlled such that the various valves in each channel and pump are controlled in an automated manner to extract each protein of interest and deliver each protein of interest into its own storage vessel.

公开(公告)号：US20060188991A1

公开(公告)日：2006-08-24

申请号：US11410572

申请日：2006-04-24

申请人： Alison McCormick ,  Mark Smith ,  Kenneth Palmer ,  John Lindbo ,  Long Nguyen ,  Gregory Pogue

发明人： Alison McCormick ,  Mark Smith ,  Kenneth Palmer ,  John Lindbo ,  Long Nguyen ,  Gregory Pogue

IPC分类号： C12N15/86 ,  C12N7/00 ,  A01H1/00

CPC分类号： C07K14/005 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/385 ,  A61K2039/5258 ,  A61K2039/55516 ,  A61K2039/6075 ,  A61K2039/627 ,  A61K2039/64 ,  C07K14/4748 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20034 ,  C12N2760/16122 ,  C12N2760/18022 ,  C12N2770/00022 ,  C12N2770/00023

摘要： Herein-described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different encapsidation intermediate such that the reassembled VLP displays more than one peptide or nucleic acid. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

摘要翻译： 本文描述了制备由重组病毒颗粒（VLP）制成的疫苗的各种方法。 首先，将VLP分解成壳化中间种群。 每个壳化中间体经历例如独特的肽或核酸部分的化学共轭以形成分开的群体。 此后，将来自不同群体的几种（一种或多种）不同的壳化中间体中的每一种的预定量混合并连接，形成围绕核酸核心的完整的VLP，其由不同的包封中间体组成，使得重新组装的VLP显示 多于一种肽或核酸。 核酸可以作为单独的支架起作用，或者可以被工程化以在真核细胞中表达免疫调节蛋白。

公开(公告)号：US20060018900A1

公开(公告)日：2006-01-26

申请号：US11209592

申请日：2005-08-22

申请人： Alison McCormick ,  Daniel Tuse ,  Stephen Reinl ,  John Lindbo ,  Thomas Turpen

发明人： Alison McCormick ,  Daniel Tuse ,  Stephen Reinl ,  John Lindbo ,  Thomas Turpen

IPC分类号： A61K39/395 ,  A61K48/00 ,  C40B40/08

CPC分类号： C07K16/00 ,  A61K2039/505 ,  A61K2039/55522 ,  A61K2039/5555 ,  A61K2039/55577 ,  C07K2317/13 ,  C07K2317/21 ,  C07K2317/622 ,  C12N15/8242 ,  C12N15/8258

摘要： A polypeptide self-antigen useful in a tumor-specific vaccine mimics one or more epitopes of an antigen uniquely expressed by cells of the tumor. The polypeptide is preferably produced in a plant that has been transformed or transfected with nucleic acid encoding the polypeptide and is obtainable from the plant in correctly folded, preferably soluble form without a need for denaturation and renaturation. This plant-produced polypeptide is immunogenic without a need for exogenous adjuvants or other immunostimulatory materials. The polypeptide is preferably an scFv molecule that bears the idiotype of the surface immunoglobulin of a non-Hodgkin's (or B cell) lymphoma. Upon administration to a subject with lymphoma, the plant-produced, tumor-unique scFv polypeptide induces an idiotype-specific antibody or cell-mediated immune response against the lymphoma.

摘要翻译： 可用于肿瘤特异性疫苗的多肽自身抗原模拟由肿瘤细胞唯一表达的抗原的一个或多个表位。 多肽优选在已经用编码多肽的核酸转化或转染的植物中产生，并且可以以正确折叠，优选可溶形式从植物获得，而不需要变性和复性。 该植物产生的多肽是免疫原性的，不需要外源性佐剂或其他免疫刺激材料。 多肽优选是具有非霍奇金（或B细胞）淋巴瘤的表面免疫球蛋白的独特型的scFv分子。 当给予具有淋巴瘤的受试者时，植物产生的肿瘤独特的scFv多肽诱导针对淋巴瘤的独特型特异性抗体或细胞介导的免疫应答。

公开(公告)号：US20080032346A1

公开(公告)日：2008-02-07

申请号：US11768157

申请日：2007-06-25

申请人： Hal Padgett ,  John Lindbo ,  Wayne Fitzmaurice

发明人： Hal Padgett ,  John Lindbo ,  Wayne Fitzmaurice

IPC分类号： C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/102 ,  C12N15/1027

摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

摘要翻译： 我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。 该方法使用相反链的退火以形成具有错配的多核苷酸双链体。 将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。 并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。 任选地连接得到的多核苷酸。 产生几种变异多核苷酸。 在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。 在退火异源链之前，母体多核苷酸不需要切割成片段。 因此，不需要重新组装。

公开(公告)号：US20050282263A1

公开(公告)日：2005-12-22

申请号：US11090497

申请日：2005-03-25

申请人： Alison McCormick ,  Mark Smith ,  Kenneth Palmer ,  John Lindbo ,  Long Nguyen ,  Gregory Pogue

发明人： Alison McCormick ,  Mark Smith ,  Kenneth Palmer ,  John Lindbo ,  Long Nguyen ,  Gregory Pogue

IPC分类号： C12N7/00 ,  C12N7/04 ,  C12N15/86

CPC分类号： C12N7/00 ,  A61K2039/5258 ,  C12N2710/20022 ,  C12N2770/00023

摘要： Herein described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into coat proteins or encapsidation intermediate populations. Each population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different coat proteins or encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different coat proteins such that the reassembled VLP displays more than one peptide or other molecule. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

摘要翻译： 本文描述了制备由重新组装的病毒样颗粒（VLP）制成的疫苗的各种方法。 首先，将VLP分解成外壳蛋白或衣壳化中间种群。 每个群体经历例如独特的肽或核酸部分的化学共轭以形成分开的群体。 此后，将来自不同群体的几种（一种或多种）不同外壳蛋白或壳化中间体中的每一种的预定量混合并连接，形成围绕核酸核心的完整VLP，其由不同的外壳蛋白组成，使得 重组VLP显示多于一个肽或其他分子。 核酸可以作为单独的支架起作用，或者可以被工程化以在真核细胞中表达免疫调节蛋白。

公开(公告)号：US20050175590A1

公开(公告)日：2005-08-11

申请号：US10624193

申请日：2003-07-21

申请人： Wayne Fitzmaurice ,  Gregory Pogue ,  John Lindbo

发明人： Wayne Fitzmaurice ,  Gregory Pogue ,  John Lindbo

IPC分类号： A01H5/00 ,  C07K14/08 ,  C12N5/10 ,  C12N15/09 ,  C12N15/40 ,  C12N15/82 ,  C12N15/86 ,  C07H21/04 ,  A61K48/00 ,  C12N7/00

CPC分类号： C07K14/005 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/86 ,  C12N2770/00022 ,  C12N2770/00043

摘要： The present invention provides nucleic acid sequences having an altered viral movement protein and 126/183 kDa replicase proteins further characterized in its ability tostabilize a transgene contained in a virus that expresses the altered movement protein. The present invention also provides viral vectors expressing the altered movement protein, cells transformed with the vectors, and host plants infected by the viral vectors.

摘要翻译： 本发明提供了具有改变的病毒运动蛋白质和126/183kDa复制酶蛋白质的核酸序列，其进一步的特征在于其能够稳定包含在表达改变的运动蛋白质的病毒中的转基因。 本发明还提供表达改变的运动蛋白的病毒载体，用载体转化的细胞，以及被病毒载体感染的宿主植物。

公开(公告)号：US20050153283A1

公开(公告)日：2005-07-14

申请号：US10226372

申请日：2002-08-21

申请人： Hal Padgett ,  John Lindbo ,  Wayne Fitzmaurice

发明人： Hal Padgett ,  John Lindbo ,  Wayne Fitzmaurice

IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/1027

摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

摘要翻译： 我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。 该方法使用相反链的退火以形成具有错配的多核苷酸双链体。 将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。 并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。 任选地连接得到的多核苷酸。 产生几种变异多核苷酸。 在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。 在退火异源链之前，母体多核苷酸不需要切割成片段。 因此，不需要重新组装。

公开(公告)号：US20050005319A1

公开(公告)日：2005-01-06

申请号：US10893056

申请日：2004-07-16

申请人： Guy della-Cioppa ,  Robert Erwin ,  Wayne Fitzmaurice ,  Kathleen Hanley ,  Moaro Kumagai ,  John Lindbo ,  David McGee ,  Hal Padgett ,  Gregory Pogue

发明人： Guy della-Cioppa ,  Robert Erwin ,  Wayne Fitzmaurice ,  Kathleen Hanley ,  Moaro Kumagai ,  John Lindbo ,  David McGee ,  Hal Padgett ,  Gregory Pogue

IPC分类号： A01H1/04 ,  A01H5/12 ,  C12N15/10 ,  C12N15/83 ,  C12Q1/68 ,  A01H1/00 ,  C12N15/82 ,  C12N15/87

CPC分类号： C12N15/1079 ,  A01H1/04 ,  A01H5/12 ,  C12N15/1034 ,  C12Q1/68

摘要： The present invention provides methods for rapidly determining the function of nucleic acid sequences by transfecting the same into a host organism to effect expression. Phenotypic and biochemical changes produced thereby are then analyzed to ascertain the function of the nucleic acids which have been transfected into the host organism. The invention also provides methods for silencing endogenous genes by transfecting hosts with nucleic acid sequences to effect expression of the same. The present invention also provides methods for selecting desired functions of RNAs and proteins by the use of virus vectors to express libraries of nucleic acid sequence variants. Moreover, the present invention provides methods for inhibiting an endogenous protease of a plant host.

摘要翻译： 本发明提供了通过将其转染宿主生物来进行表达来快速测定核酸序列的功能的方法。 然后分析由此产生的表型和生物化学变化以确定已经转染到宿主生物体中的核酸的功能。 本发明还提供了通过用核酸序列转染宿主以使其表达而沉默内源基因的方法。 本发明还提供了通过使用病毒载体来表达核酸序列变体文库来选择RNA和蛋白质的所需功能的方法。 此外，本发明提供了抑制植物宿主的内源性蛋白酶的方法。