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    Flexible vaccine assembly and vaccine delivery platform
    5.
    发明申请
    Flexible vaccine assembly and vaccine delivery platform 审中-公开
    灵活的疫苗组装和疫苗交付平台

    公开(公告)号：US20050282263A1

    公开(公告)日：2005-12-22

    申请号：US11090497

    申请日：2005-03-25

    申请人： Alison McCormick ,  Mark Smith ,  Kenneth Palmer ,  John Lindbo ,  Long Nguyen ,  Gregory Pogue

    发明人： Alison McCormick ,  Mark Smith ,  Kenneth Palmer ,  John Lindbo ,  Long Nguyen ,  Gregory Pogue

    IPC分类号： C12N7/00 ,  C12N7/04 ,  C12N15/86

    CPC分类号： C12N7/00 ,  A61K2039/5258 ,  C12N2710/20022 ,  C12N2770/00023

    摘要： Herein described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into coat proteins or encapsidation intermediate populations. Each population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different coat proteins or encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different coat proteins such that the reassembled VLP displays more than one peptide or other molecule. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

    摘要翻译： 本文描述了制备由重新组装的病毒样颗粒（VLP）制成的疫苗的各种方法。 首先，将VLP分解成外壳蛋白或衣壳化中间种群。 每个群体经历例如独特的肽或核酸部分的化学共轭以形成分开的群体。 此后，将来自不同群体的几种（一种或多种）不同外壳蛋白或壳化中间体中的每一种的预定量混合并连接，形成围绕核酸核心的完整VLP，其由不同的外壳蛋白组成，使得 重组VLP显示多于一个肽或其他分子。 核酸可以作为单独的支架起作用，或者可以被工程化以在真核细胞中表达免疫调节蛋白。

    Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector
    8.
    发明申请
    Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector 审中-公开
    通过植物病毒载体对单子叶植物中的基因表达和外源蛋白的表达的细胞质抑制

    公开(公告)号：US20050009012A1

    公开(公告)日：2005-01-13

    申请号：US10913536

    申请日：2004-08-06

    申请人： Steven Holzberg ,  Gregory Pogue

    发明人： Steven Holzberg ,  Gregory Pogue

    IPC分类号： C07K14/08 ,  C12N15/82 ,  C12Q1/70 ,  A01H1/00 ,  C07H21/04 ,  C07K14/005 ,  C12N5/04

    CPC分类号： C07K14/005 ,  C12N15/8216 ,  C12N15/8218 ,  C12N2770/00022

    摘要： The present invention relates to recombinant viral vectors encoding a transcriptional unit, that encodes a fusion protein, or a foreign protein or a gene of interest to be silenced, which can be expressed in a host. The present invention also relates to the use of these recombinant viral vectors to express a fusion protein, a foreign protein, to silence a gene of interest in a host. The present invention also relates to the use of these recombinant viral vectors to screen a cDNA or genomic library in order to correlate a nucleotide sequence with a phenotypic or biochemical change.

    摘要翻译： 本发明涉及编码融合蛋白的转录单位的重组病毒载体，或可以在宿主中表达的待沉默的外源蛋白或感兴趣的基因。 本发明还涉及这些重组病毒载体在宿主中表达融合蛋白，一种外来蛋白质以使感兴趣的基因沉默的用途。 本发明还涉及这些重组病毒载体筛选cDNA或基因组文库以使核苷酸序列与表型或生物化学变化相关联的用途。

    Retrofit Jig System for a Truss Assembly Table
    9.
    发明申请
    Retrofit Jig System for a Truss Assembly Table 审中-公开
    桁架装配台改装夹具系统

    公开(公告)号：US20080084014A1

    公开(公告)日：2008-04-10

    申请号：US11864436

    申请日：2007-09-28

    申请人： Kieth Godwin ,  Gerald McCoombe ,  Gregory Pogue ,  Ferdie Tenshler ,  Jack Dodd ,  Adam McClenahan

    发明人： Kieth Godwin ,  Gerald McCoombe ,  Gregory Pogue ,  Ferdie Tenshler ,  Jack Dodd ,  Adam McClenahan

    IPC分类号： B27F7/15

    CPC分类号： B25B11/02

    摘要： The invention is directed toward a retrofit jig system for use with an existing truss assembly table having multiple planks extending lengthwise along the table. The jig system includes a motor and jig assembly for moving a puck lengthwise along the truss assembly table between adjacent planks of the table. A support member supports the screw between the adjacent planks. The support member is adjustable to allow the puck to move past the support member without interference.

    摘要翻译： 本发明涉及一种用于与现有的桁架组装台一起使用的改装夹具系统，其具有沿桌子纵向延伸的多个木板。 夹具系统包括马达和夹具组件，用于沿着桁架组件台沿着桌子的相邻板之间纵向移动圆盘。 支撑构件支撑相邻板之间的螺钉。 支撑构件是可调节的，以允许圆盘不受干扰地移动经过支撑构件。

    Rolling circle replicon expression vector
    10.
    发明申请
    Rolling circle replicon expression vector 审中-公开

    公开(公告)号：US20060024821A1

    公开(公告)日：2006-02-02

    申请号：US11231725

    申请日：2005-09-20

    申请人： Kenneth Palmer ,  Gregory Pogue

    发明人： Kenneth Palmer ,  Gregory Pogue

    IPC分类号： C12N15/86

    CPC分类号： C07K14/005 ,  A01K2217/05 ,  C12N15/79 ,  C12N15/86 ,  C12N2750/00022 ,  C12N2750/12043 ,  C12N2800/108 ,  C12N2830/42

    摘要： A rolling circle DNA replicon which replicates in a host eukaryotic cell is disclosed which has a truncated replication cycle. The rolling circle DNA replicon comprises the following elements present on the same DNA molecule. It contains a Rep gene open reading frame from a virus belonging to the viral taxonomic families Geminiviridae, Circoviridae or genus Nanovirus. The Rep gene open reading frame is placed under transcriptional control of a promoter, which is placed 5′ of the gene. Any sequences that are required to be present in cis on the rolling circle DNA replicon in order that the Rep protein might promote replication of the rolling circle DNA replicon are included. An expression cassette for expression of an ancillary protein that is capable of creating a cellular environment permissive for replication of the rolling circle DNA replicon in the host cell of interest is also included. At least one expression cassette with an RNA polymerase II promoter, a multiple cloning site, and transcription termination and polyadenylation signals suitable for transcription of RNA molecules not normally intrinsic to a geminiviral, circoviral or nanoviral genome is also included.