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18 条记录 (耗时 0.031 秒)

    Methods of preparing compositions comprising chemicals capable of transcriptional modulators
    1.
    发明授权
    Methods of preparing compositions comprising chemicals capable of transcriptional modulators 失效
    制备包含能够转录调节剂的化学品的组合物的方法

    公开(公告)号：US06589733B1

    公开(公告)日：2003-07-08

    申请号：US08463398

    申请日：1995-06-05

    申请人： J. Gordon Foulkes ,  Robert Franco ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    发明人： J. Gordon Foulkes ,  Robert Franco ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    IPC分类号： C12Q168

    CPC分类号： A61K31/7105 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/42 ,  C12N2830/85 ,  C12N2840/20 ,  C12Q1/6897

    摘要： The present invention provides a method of transcriptionally modulating the expression of a gene of interest, the expression of which is associated with a defined physiological or pathological effect within a multicellular organism. The method comprises contacting a cell which is capable of expressing the gene with an amount of a molecule effective to transcriptionally modulate expression of the gene and thereby affect the level of the protein encoded by the gene which is expressed by the cell. Molecules useful in the practice of the invention are characterized as follows (a) do not naturally occur in the cell, (b) specifically transcriptionally modulate expression of the gene of interest, and (c) bind to DNA or RNA or bind to a protein through a domain of such protein which is not a ligand binding domain of a receptor which naturally occurs in the cell, the binding of a ligand to which ligand binding domain is normally associated with the defined physiological or pathological effect.

    摘要翻译： 本发明提供了一种转录调节感兴趣的基因的表达的方法，其表达与多细胞生物体内具有规定的生理或病理作用相关。 该方法包括使能够表达基因的细胞与一定量的有效转录调节基因表达的分子接触，从而影响由细胞表达的基因编码的蛋白质的水平。 在本发明的实践中有用的分子的特征如下（a）天然不发生在细胞中，（b）特异地转录调节目的基因的表达，和（c）结合DNA或RNA或结合蛋白质 通过这种蛋白质的结构域，其不是天然存在于细胞中的受体的配体结合结构域，配体结合结构域通常与所定义的生理或病理作用相关联。

    Tissue-derived tumor growth inhibitors, methods of preparation and uses thereof
    2.
    发明授权
    Tissue-derived tumor growth inhibitors, methods of preparation and uses thereof 失效
    组织衍生的肿瘤生长抑制剂，其制备方法及其应用

    公开(公告)号：US06425769B1

    公开(公告)日：2002-07-30

    申请号：US08457097

    申请日：1995-06-01

    申请人： Kenneth K. Iwata ,  John R. Stephenson ,  Peter ten Dijke

    发明人： Kenneth K. Iwata ,  John R. Stephenson ,  Peter ten Dijke

    IPC分类号： C07H2104

    CPC分类号： C07K16/22 ,  A61K35/44 ,  A61K35/50 ,  A61K38/00 ,  C07K14/495 ,  C07K14/52 ,  C07K16/24 ,  C07K2319/00 ,  G01N33/74

    摘要： The invention provides a recombinant nucleic acid vector comprising a nucleic acid encoding a polypeptide of 112 amino acids having the amino acid sequence shown in FIG. 29 beginning with alanine at position 1 and ending with serine at position 112. The sequence of FIG. 29 represents a member of the TGF-&bgr; family of tumor growth factors. The invention also provides a method for producing a protein which comprises culturing the host cell under conditions suitable to express the protein in the host cell and recovering the protein so produced.

    摘要翻译： 本发明提供了包含编码具有图11所示氨基酸序列的112个氨基酸的多肽的核酸的重组核酸载体。 29从位置1处的丙氨酸开始并以位置112处的丝氨酸结束。 29代表肿瘤生长因子的TGF-β家族成员。 本发明还提供了一种生产蛋白质的方法，其包括在适于在宿主细胞中表达蛋白质的条件下培养宿主细胞并回收如此产生的蛋白质。

    Methods of transcriptionally modulating gene expression and of discovering chemicals capable as gene expression modulators
    6.
    发明授权
    Methods of transcriptionally modulating gene expression and of discovering chemicals capable as gene expression modulators 失效
    转录调控基因表达和发现能够作为基因表达调节剂的化学物质的方法

    公开(公告)号：US5976793A

    公开(公告)日：1999-11-02

    申请号：US683455

    申请日：1996-07-18

    申请人： J. Gordon Foulkes ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    发明人： J. Gordon Foulkes ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    IPC分类号： A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C07K14/775 ,  C12N15/67 ,  C12N15/79 ,  C12N15/85 ,  C12P19/34 ,  C12Q1/68 ,  C12N5/10

    CPC分类号： A61K31/7068 ,  A61K48/00 ,  C07K14/775 ,  C12N15/67 ,  C12Q1/6886 ,  C12Q1/6897 ,  C12Q2600/136

    摘要： The present invention provides a method of transcriptionally modulating the expression of a gene-of-interest. The method comprises contacting a cell which is capable of expressing the gene with an amount of a molecule effective to transcriptionally modulate expression of the gene and thereby affect the level of the protein encoded by the gene which is expressed by the cell. Molecules useful in the practice of the invention are characterized as follows (a) do not naturally occur in the cell, (b) bind to DNA or RNA or bind to a protein through a domain of such protein which is not a ligand binding domain of a receptor which naturally occurs in the cell. Additionally, this invention provides a method for determining whether a molecule known to be a modulator of protein biosynthesis is capable of transcriptionally modulating expression of a gene-of-interest.

    摘要翻译： 本发明提供了转录调节感兴趣基因表达的方法。 该方法包括使能够表达基因的细胞与一定量的有效转录调节基因表达的分子接触，从而影响由细胞表达的基因编码的蛋白质的水平。 在本发明的实践中有用的分子的特征如下（a）天然不发生在细胞中，（b）通过不属于这种蛋白质的配体结合域的蛋白质的结构域结合DNA或RNA或结合蛋白质 天然存在于细胞中的受体。 此外，本发明提供了一种用于确定已知是蛋白质生物合成调节剂的分子是否能够转录调节感兴趣的基因的表达的方法。

    Deoxyribonucleic acid molecules useful as probes for detecting oncogenes incorporated into chromosomal DNA
    7.
    发明授权
    Deoxyribonucleic acid molecules useful as probes for detecting oncogenes incorporated into chromosomal DNA 失效
    脱氧核糖核酸分子可用作检测引入染色体DNA的癌基因的探针

    公开(公告)号：US4681840A

    公开(公告)日：1987-07-21

    申请号：US571911

    申请日：1984-01-18

    申请人： John R. Stephenson ,  John Groffen ,  Nora Heisterkamp

    发明人： John R. Stephenson ,  John Groffen ,  Nora Heisterkamp

    IPC分类号： G01N33/50 ,  C07H21/04 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566 ,  G01N33/58 ,  G01N33/48 ,  G01N33/52

    CPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/156

    摘要： A single-stranded deoxyribonucleic acid molecule having a length of less than about 25 kb comprises at least three distinct nucleotide sequences which are the sites for incorporation into a chromosome of a deoxyribonucleic acid molecule encoding a deleterious gene. Deoxyribonucleic acid probes have been prepared from such molecules and are useful as hybridization probes for detecting chromosomal deoxyribonucleic acid which has a deoxyribonucleic acid molecule encoding a deleterious gene, i.e. oncogene, incorporated therein.A single-stranded deoxyribonucleic acid molecule derived from human chromosome 22 which is about 5.8 kb in length contains sites for incorporation of a deoxyribonucleic acid molecule encoding the oncogene c-abl derived from human chromosome 9. Deoxyribonucleic acid probes have been prepared from this molecule and used to detect the abnormal Philadelphia chromosome and chronic myelocytic leukemia.

    摘要翻译： 长度小于约25kb的单链脱氧核糖核酸分子包含至少三个不同的核苷酸序列，其是用于掺入编码有害基因的脱氧核糖核酸分子染色体的位点。 已经从这样的分子制备脱氧核糖核酸探针，并且可用作检测染色体脱氧核糖核酸的杂交探针，其具有编码有害基因（即癌基因）的脱氧核糖核酸分子。 衍生自长度为约5.8kb的人染色体22的单链脱氧核糖核酸分子包含用于掺入编码源自人染色体9的致癌基因c-abl的脱氧核糖核酸分子的位点。已经从该分子制备了脱氧核糖核酸探针 用于检测费城染色体异常和慢性骨髓性白血病。

    Tissue-derived tumor growth inhibitor
    8.
    发明授权
    Tissue-derived tumor growth inhibitor 失效
    组织来源的肿瘤生长抑制剂

    公开(公告)号：US06586394B1

    公开(公告)日：2003-07-01

    申请号：US08188197

    申请日：1994-01-27

    申请人： Kenneth K. Iwata ,  John R. Stephenson ,  Leslie I. Gold

    发明人： Kenneth K. Iwata ,  John R. Stephenson ,  Leslie I. Gold

    IPC分类号： A61K3818

    CPC分类号： C07K14/52 ,  A61K35/44 ,  A61K35/50 ,  A61K38/00 ,  C07K14/495 ,  C07K16/22 ,  C07K16/24 ,  C07K2319/00 ,  G01N33/74

    摘要： This invention is directed to a chromatographically recovered polypeptide having the N-terminal amino acid sequence Ala-Leu-Asp-Thr-Asn-Tyr-Cys-Phe-Arg-Asn-Leu-Glu-Clu-Asn-Cys-Cys-Val. This polypeptide is known as TGI, TGI-1 and TGI-2. It is also referred to as TGF-&bgr;3. The invention is also directed to a compositions which comprises the chromatographically recovered polypeptide. The invention also provides a pharmaceutical compositions to inhibit the growth of epithelial cells or heal a wound or treat a burn consisting of the chromatographically recovered polypeptide. The invention is also directed to methods which comprise administering to a subject an effective amount of the chromatographically recovered so as to thereby inhibit the growth of epithelial cells, or heal the wound or treat the burn.

    摘要翻译： 本发明涉及具有N-末端氨基酸序列Ala-Leu-Asp-Thr-Asn-Tyr-Cys-Phe-Arg-Asn-Leu-Glu-Clu-Asn-Cys-Cys-Val的色谱回收多肽 。 该多肽称为TGI，TGI-1和TGI-2。 它也称为TGF-β3。 本发明还涉及包含色谱回收多肽的组合物。 本发明还提供了抑制上皮细胞生长或愈合伤口或治疗由色谱回收的多肽组成的烧伤的药物组合物。 本发明还涉及包括向受试者施用有效量的色谱回收的方法，从而抑制上皮细胞的生长，或愈合伤口或治疗烧伤。

    Methods of specifically transcriptionally modulating the expression of gene of interest
    10.
    发明授权
    Methods of specifically transcriptionally modulating the expression of gene of interest 失效
    特异性转录调控感兴趣的基因表达的方法

    公开(公告)号：US6136779A

    公开(公告)日：2000-10-24

    申请号：US778754

    申请日：1997-01-06

    申请人： J. Gordon Foulkes ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    发明人： J. Gordon Foulkes ,  Franz Leichtfried ,  Christian Pieler ,  John R. Stephenson

    IPC分类号： A61K48/00 ,  C07H21/02 ,  C07H21/04 ,  C07K14/775 ,  C12N15/67 ,  C12N15/79 ,  C12N15/85 ,  C12P19/34 ,  C12Q1/68 ,  A01K31/00 ,  C12P21/00

    CPC分类号： A61K31/7068 ,  A61K48/00 ,  C07K14/775 ,  C12N15/67 ,  C12Q1/6886 ,  C12Q1/6897 ,  C12Q2600/136

    摘要： The present invention provides a method of transcriptionally modulating the expression of a gene-of-interest. The method comprises contacting a cell which is capable of expressing the gene with an amount of a molecule effective to transcriptionally modulate expression of the gene and thereby affect the level of the protein encoded by the gene which is expressed by the cell. Molecules useful in the practice of the invention are characterized as follows (a) do not naturally occur in the cell, (b) bind to DNA or RNA or bind to a protein through a domain of such protein which is not a ligand binding domain of a receptor which naturally occurs in the cell. Additionally, this invention provides a method for determining whether a molecule known to be a modulator of protein biosynthesis is capable of transcriptionally modulating expression of a gene-of-interest.

    摘要翻译： 本发明提供了转录调节感兴趣基因表达的方法。 该方法包括使能够表达基因的细胞与一定量的有效转录调节基因表达的分子接触，从而影响由细胞表达的基因编码的蛋白质的水平。 在本发明的实践中有用的分子的特征如下（a）天然不发生在细胞中，（b）通过不属于这种蛋白质的配体结合域的蛋白质的结构域结合DNA或RNA或结合蛋白质 天然存在于细胞中的受体。 此外，本发明提供了一种用于确定已知是蛋白质生物合成调节剂的分子是否能够转录调节感兴趣的基因的表达的方法。