公开(公告)号：US06589786B1

公开(公告)日：2003-07-08

申请号：US09489116

申请日：2000-01-21

Applicant: Joseph A. Mangano ,  Henry M. Eppich

Inventor： Joseph A. Mangano ,  Henry M. Eppich

IPC: C12N508

CPC classification number: C12N5/0087 ,  C12M35/02 ,  C12M47/04 ,  C12N1/02 ,  C12N1/06 ,  C12N5/0093 ,  C12N13/00

Abstract: The present invention involves methods and devices which enable discrete objects having a conducting inner core, surrounded by a dielectric membrane to be selectively inactivated by electric fields via irreversible breakdown of their dielectric membrane. One important application of the invention is in the selection, purification, and/or purging of desired or undesired biological cells from cell suspensions. According to the invention, electric fields can be utilized to selectively inactivate and render non-viable particular subpopulations of cells in a suspension, while not adversely affecting other desired subpopulations. According to the inventive methods, the cells can be selected on the basis of intrinsic or induced differences in a characteristic electroporation threshold, which can depend, for example, on a difference in cell size and/or critical dielectric membrane breakdown voltage. The invention enables effective cell separation without the need to employ undesirable exogenous agents, such as toxins or antibodies. The inventive method also enables relatively rapid cell separation involving a relatively low degree of trauma or modification to the selected, desired cells. The inventive method has a variety of potential applications in clinical medicine, research, etc., with two of the more important foreseeable applications being stem cell enrichment/isolation, and cancer cell purging.

公开(公告)号：US4211983A

公开(公告)日：1980-07-08

申请号：US901600

申请日：1978-05-01

Applicant: Jack D. Daugherty ,  Jonah H. Jacob ,  Joseph A. Mangano

Inventor： Jack D. Daugherty ,  Jonah H. Jacob ,  Joseph A. Mangano

IPC: H01S3/097

CPC classification number: H01S3/09707

Abstract: Improved electron beam driven gas laser method and apparatus with the achievement of high single pulse energies therefore enabled by establishment of a magnetic field in a direction substantially parallel to the desired direction of electron beam propagation into a gas. The applied magnetic field is made substantially uniform and sized to exceed that of the self induced magnetic field of the current established by the electron beam, alone or in combination with a discharge current (optionally) established in the gas.

Abstract translation: 因此，通过在基本上平行于电子束传播到气体的期望方向的方向上建立磁场来实现改进的电子束驱动气体激光方法和实现高单脉冲能量的装置。 所施加的磁场被制成基本上均匀且尺寸超过由电子束建立的电流的自身感应磁场的单独或与在气体中建立的放电电流（可选地）组合的尺寸。

公开(公告)号：US6043066A

公开(公告)日：2000-03-28

申请号：US148620

申请日：1998-09-04

Applicant: Joseph A. Mangano ,  Henry M. Eppich

Inventor： Joseph A. Mangano ,  Henry M. Eppich

IPC: C12M3/00 ,  C12N1/02 ,  C12N1/06 ,  C12N5/00 ,  C12N5/06 ,  C12N5/08 ,  C12N13/00

CPC classification number: C12N5/0087 ,  C12M35/02 ,  C12M47/04 ,  C12N1/02 ,  C12N1/06 ,  C12N13/00 ,  C12N5/0093

Abstract: The present invention involves methods and devices which enable discrete objects having a conducting inner core, surrounded by a dielectric membrane to be selectively inactivated by electric fields via irreversible breakdown of their dielectric membrane. One important application of the invention is in the selection, purification, and/or purging of desired or undesired biological cells from cell suspensions. According to the invention, electric fields can be utilized to selectively inactivate and render non-viable particular subpopulations of cells in a suspension, while not adversely affecting other desired subpopulations. According to the inventive methods, the cells can be selected on the basis of intrinsic or induced differences in a characteristic electroporation threshold; which can depend, for example, on a difference in cell size and/or critical dielectric membrane breakdown voltage. The invention enables effective cell separation without the need to employ undesirable exogenous agents, such as toxins or antibodies. The inventive method also enables relatively rapid cell separation involving a relatively low degree of trauma or modification to the selected, desired cells. The inventive method has a variety of potential applications in clinical medicine, research, etc., with two of the more important foreseeable applications being stem cell enrichment/isolation, and cancer cell purging.

Abstract translation: 本发明涉及能够使具有导电内芯的离散物体的方法和装置，其被介电膜包围，以通过其电场通过其介电膜的不可逆击穿来选择性地失活。 本发明的一个重要应用是从细胞悬浮液中选择，纯化和/或清除所需或不需要的生物细胞。 根据本发明，可以使用电场来选择性地使悬浮液中的非活细胞细胞群失活并使其呈现，同时不影响其它所需亚种群。 根据本发明的方法，可以基于特征电穿孔阈值的固有或诱导差异来选择细胞; 这可以取决于例如电池尺寸和/或临界电介质膜击穿电压的差异。 本发明能够有效地进行细胞分离，而不需要使用不需要的外源性试剂，例如毒素或抗体。 本发明的方法还使得相对快速的细胞分离涉及对所选择的所需细胞的相对低的创伤或修饰程度。 本发明方法在临床医学，研究等方面具有多种潜在的应用，其中两个更重要的可预见的应用是干细胞富集/分离和癌细胞清除。

公开(公告)号：US07002168B2

公开(公告)日：2006-02-21

申请号：US10442544

申请日：2003-05-21

Applicant: Jonah Jacob ,  Joseph A. Mangano ,  James Moran ,  Alexander Bykanov ,  Rodney Petr ,  Mordechai Rokni

Inventor： Jonah Jacob ,  Joseph A. Mangano ,  James Moran ,  Alexander Bykanov ,  Rodney Petr ,  Mordechai Rokni

IPC: H01J35/00

CPC classification number: H05G2/003 ,  H05G2/006 ,  H05H1/48

Abstract: A dense plasma focus radiation source for generating EUV radiation using Lithium vapor and including a coaxially disposed anode and cathode. The invention includes methods and apparatuses for enhancing the efficiency of EUV radiation production, for protecting, cooling and extending the life of the anode and cathode, for protecting and shielding collecting optics from debris and pressure disturbances in the discharge chamber, and for feeding Lithium into the discharge chamber.

Abstract translation: 一种致密的等离子体聚焦辐射源，用于使用锂蒸汽产生EUV辐射，并包括同轴设置的阳极和阴极。 本发明包括用于提高EUV辐射生产效率的方法和装置，用于保护，冷却和延长阳极和阴极的寿命，用于保护和屏蔽聚集光学元件不受放电室中的碎片和压力扰动的影响，并将锂进入 放电室。

公开(公告)号：US07180921B2

公开(公告)日：2007-02-20

申请号：US10914613

申请日：2004-08-09

Applicant: Joseph A. Mangano ,  Rodney Petr

Inventor： Joseph A. Mangano ,  Rodney Petr

IPC: H01S3/00

CPC classification number: H01S5/0428 ,  H01S5/06808 ,  H01S5/06825

Abstract: A method and apparatus for driving a laser diode source, such as a laser diode or a laser diode array. The driver controlling current in response to a signal indicative of excessive current or current density. The signal may be derived from the drive current, the voltage across the laser diode source or the impedance of the laser diode source. The circuit may be pulsed using a switch, such as a GCT. The current to generate drive the laser diode source provided by a capacitive or inductive charging circuit.

Abstract translation: 用于驱动激光二极管源的方法和装置，例如激光二极管或激光二极管阵列。 驱动器响应于指示过大电流或电流密度的信号来控制电流。 信号可以从驱动电流，激光二极管源上的电压或激光二极管源的阻抗导出。 电路可以使用诸如GCT的开关脉冲。 产生驱动由电容或感应充电电路提供的激光二极管源的电流。

公开(公告)号：US4203078A

公开(公告)日：1980-05-13

申请号：US794401

申请日：1977-05-06

Applicant: Jack D. Daugherty ,  Jonah H. Jacob ,  Joseph A. Mangano

Inventor： Jack D. Daugherty ,  Jonah H. Jacob ,  Joseph A. Mangano

IPC: H01S3/097 ,  H01S3/225

CPC classification number: H01S3/225 ,  H01S3/09707

Abstract: Apparatus for and method of operating a laser wherein a discharge is produced preferably in a high pressure lasing gaseous mixture comprising at least one suitable first gaseous species capable of providing an excited state which has a finite probability of being ionized and a molecular second gaseous species having a capability for attaching electrons to form negative ions. The gaseous mixture may, for example, comprise argon, neon, helium, xenon, krypton or a metal vapor such as mercury as the first species and, for example, hydrogen iodide, carbon tetrachloride, bromine, iodine or fluorine as the second species. A buffer gas such as, for example, argon, helium or neon may also be used. The discharge is produced by means of an electron beam and an electric field. The discharge resulting from the application of the electric field heats secondary electrons produced by the electron beam to an energy level sufficient to make excited states. Thus, for a mixture comprising argon, krypton and fluorine, for example, the heated secondary electrons pump at least some of the argon and the krypton to the metastable state. The excited argon transfers energy to the krypton to form additional excited krypton which, in turn, reacts with the fluorine to form excited krypton fluoride molecules. The krypton fluoride then dissociates or decays upon the emission of spontaneous or stimulated radiation. At power input levels where the electron density remains constant in time for a constant electric field, efficient discharge pumping of the excited states is provided when the fractional excited state population is kept small. Stable discharge operation is achieved when the lasing mixture contains an amount of the second species gas sufficient to provide an attachment rate n times the equilibrium ionization rate where n is the number of electron excitations which causes ionization of the first species.