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公开(公告)号：US10688694B2
公开(公告)日：2020-06-23
申请号：US15768161
申请日：2016-10-14
申请人： UNIVERSIDAD DE LOS ANDES , CELLS FOR CELLS S.A. , Juan Pablo Acevedo , Camila Wilkens , Maroun Khoury , Christopher Rivet
IPC分类号： B29C41/22 , B29C41/14 , A61L27/34 , A61L27/50 , A61L27/38 , A61F2/04 , A61F2/06 , A61L27/18 , A61L27/20 , A61L27/22 , A61L27/26 , A61L27/36 , A61L27/54 , A61L27/58 , B32B1/08 , B32B9/02 , B29L31/00
摘要： The present invention overcomes all the above drawbacks and provides a versatile method for the fabrication of multilayer hollow tubes that uses a layer-by-layer rod dipping approach using different biomaterials. The device enables fine control over fabrication parameters, such as ascending/descending speeds, rod rotational velocity, and crosslinking or polymerization time. All these technologies allows the generation of more complex multilayer hollow tubes such as vessel-like structures, urethral grafting, prostate grafting and the like.
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公开(公告)号：US20180304502A1
公开(公告)日：2018-10-25
申请号：US15768161
申请日：2016-10-14
申请人： Juan Pablo ACEVEDO , Camila WILKENS , Maroun KHOURY , Christopher RIVET , UNIVERSIDAD DE LOS ANDES , CELLS FOR CELLS, S.P.A
IPC分类号： B29C41/22 , B32B1/08 , B32B9/02 , B29C41/14 , A61L27/22 , A61L27/54 , A61L27/38 , A61L27/34 , A61L27/18 , A61L27/26 , A61L27/58 , A61L27/50 , A61L27/36 , A61L27/20 , A61F2/04 , A61F2/06
CPC分类号： B29C41/22 , A61F2/04 , A61F2/06 , A61F2002/047 , A61F2210/0076 , A61F2240/001 , A61L27/18 , A61L27/20 , A61L27/222 , A61L27/26 , A61L27/34 , A61L27/3679 , A61L27/3808 , A61L27/3813 , A61L27/3817 , A61L27/3826 , A61L27/3834 , A61L27/3882 , A61L27/507 , A61L27/54 , A61L27/58 , A61L2300/604 , A61L2300/608 , A61L2300/64 , A61L2420/02 , A61L2420/08 , A61L2430/22 , A61L2430/32 , B29C41/14 , B29K2089/00 , B29L2031/7534 , B32B1/08 , B32B9/02 , B32B2535/00 , C08L67/04 , C08L89/06
摘要： The present invention overcomes all the above drawbacks and provides a versatile method for the fabrication of multilayer hollow tubes that uses a layer-by-layer rod dipping approach using different biomaterials. The device enables fine control over fabrication parameters, such as ascending/descending speeds, rod rotational velocity, and crosslinking or polymerization time. All these technologies allows the generation of more complex multilayer hollow tubes such as vessel-like structures, urethral grafting, prostate grafting and the like.
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3.发明授权Protein and DNA sequence encoding a cold adapted subtilisin-like activity 有权编码冷适应枯草杆菌蛋白酶样活性的蛋白质和DNA序列公开(公告)号：US08759065B2
公开(公告)日：2014-06-24
申请号：US12672226
申请日：2008-08-06
CPC分类号： C12N9/52
摘要： Nucleic acid and corresponding amino acid sequences of a cold adapted subtilisin-like activity protein, insolated from antarctic marine origin, preferably from an Antarctic bacteria (Polaribacter sp) that can be used in a variety of industrial contexts and commercial purposes including laundry detergents, food processing, leather processing and skin care products. Nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the cold adapted subtilisin-like protein are also described.
摘要翻译： 来自南极海洋来源的冷适应的枯草杆菌蛋白酶样活性蛋白质的核酸和相应的氨基酸序列，优选来自可用于各种工业环境和商业目的的南极细菌（Polaribacter sp），包括洗衣洗涤剂，食品 加工，皮革加工和护肤品。 还描述了包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生和使用冷适应的枯草杆菌蛋白酶样蛋白质的方法。
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公开(公告)号：US08679814B2
公开(公告)日：2014-03-25
申请号：US13148227
申请日：2010-02-02
CPC分类号： C12N9/2434 , C12Y302/01008
摘要： A method of preparing a cold adapted xylanase by use of recombinant DNA techniques. A nucleic acid and corresponding amino acid sequences of a cold adapted xylanase, isolated from antarctic marine origin, preferably from an Antarctic bacteria (Psychrobacter sp.) are provided. These can be used in a variety of industrial contexts and for a variety of commercial purposes including more complete hydrolysis of lignocellulosic biomass into simple sugars that can then be fermented to products, such as liquid fuels and chemical feedstocks. The enzymes are also useful in the production methods of other industries, such as the animal feed, baking, and paper industries. Nucleic acids, corresponding amino acid sequences, constructs, expression vectors or integration vectors containing the DNA molecule, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides for producing and using the Psychrobacter-derived cold adapted family GH10 xylanase-like protein are also described.
摘要翻译： 使用重组DNA技术制备冷适应木聚糖酶的方法。 提供了从南极海洋来源分离的冷适应木聚糖酶的核酸和相应的氨基酸序列，优选来自南极细菌（Psychrobacter sp。）。 这些可用于各种工业环境和各种商业目的，包括将木质纤维素生物质更完全水解成简单的糖，然后将其发酵成产物，例如液体燃料和化学原料。 酶也可用于其他行业的生产方法，如动物饲料，烘焙和造纸工业。 含有DNA分子的核酸，相应的氨基酸序列，构建体，表达载体或整合载体，以及包含多核苷酸的宿主细胞以及用于产生和使用多肽用于产生和使用来源于精神分裂杆菌的冷适应家族GH10木聚糖酶 - 也描述了类似的蛋白质。
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5.发明申请PROTEIN AND DNA SEQUENCE ENCODING A COLD ADAPTED-SUBTILISIN-LIKE ACTIVITY 有权蛋白质和DNA序列编码一种适应性好的喜树碱活性公开(公告)号：US20120264191A1
公开(公告)日：2012-10-18
申请号：US12672226
申请日：2008-08-06
CPC分类号： C12N9/52
摘要： Nucleic acid and corresponding amino acid sequences of a cold adapted subtilisin-like activity protein, insolated from antarctic marine origin, preferably from an Antarctic bacteria (Polar ibacter sp) that can be used in a variety of industrial contexts and commercial purposes including laundry detergents, food processing, leather processing and skin care products. Nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the cold adapted subtilisin-like protein are also described.
摘要翻译： 来自南极海洋来源的冷适应的枯草杆菌蛋白酶样活性蛋白质的核酸和相应的氨基酸序列，优选来自可用于各种工业环境和商业目的的南极细菌（Polar ibacter sp），包括洗衣洗涤剂， 食品加工，皮革加工和护肤品。 还描述了包含核酸序列的核酸构建体，载体和宿主细胞以及用于产生和使用冷适应的枯草杆菌蛋白酶样蛋白质的方法。
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公开(公告)号：US20110287515A1
公开(公告)日：2011-11-24
申请号：US13148227
申请日：2010-02-02
CPC分类号： C12N9/2434 , C12Y302/01008
摘要： A method of preparing a cold adapted xylanase by use of recombinant DNA techniques. A nucleic acid and corresponding amino acid sequences of a cold adapted xylanase, isolated from antarctic marine origin, preferably from an Antarctic bacteria (Psychrobacter sp.) are provided. These can be used in a variety of industrial contexts and for a variety of commercial purposes including more complete hydrolysis of lignocellulosic biomass into simple sugars that can then be fermented to products, such as liquid fuels and chemical feedstocks. The enzymes are also useful in the production methods of other industries, such as the animal feed, baking, and paper industries. Nucleic acids, corresponding amino acid sequences, constructs, expression vectors or integration vectors containing the DNA molecule, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides for producing and using the Psychrobacter-derived cold adapted family GH10 xylanase-like protein are also described.
摘要翻译： 使用重组DNA技术制备冷适应木聚糖酶的方法。 提供了从南极海洋来源分离的冷适应木聚糖酶的核酸和相应的氨基酸序列，优选来自南极细菌（Psychrobacter sp。）。 这些可用于各种工业环境和各种商业目的，包括将木质纤维素生物质更完全水解成简单的糖，然后将其发酵成产物，例如液体燃料和化学原料。 酶也可用于其他行业的生产方法，如动物饲料，烘焙和造纸工业。 含有DNA分子的核酸，相应的氨基酸序列，构建体，表达载体或整合载体，以及包含多核苷酸的宿主细胞以及用于产生和使用多肽用于产生和使用来源于精神分裂杆菌的冷适应家族GH10木聚糖酶 - 也描述了类似的蛋白质。
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