利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

172 条记录 (耗时 0.047 秒)

    Method to Quantify siRNAs, miRNAs and Polymorphic miRNAs
    2.
    发明申请
    Method to Quantify siRNAs, miRNAs and Polymorphic miRNAs 有权
    定量siRNA，miRNA和多态性miRNA的方法

    公开(公告)号：US20110212494A1

    公开(公告)日：2011-09-01

    申请号：US13015332

    申请日：2011-01-27

    申请人： Ruoying TAN ,  Caifu Chen ,  Karl J. Guegler

    发明人： Ruoying TAN ,  Caifu Chen ,  Karl J. Guegler

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6855 ,  C12Q1/6851 ,  C12Q2525/301 ,  C12Q2525/207 ,  C12Q2521/501 ,  C12Q2537/143

    摘要： The present teachings provide methods, compositions, and kits for quantifying target, polynucleotides. In some embodiments, a reverse stem-loop ligation probe is ligated to the 3′ end of a target polynucleotide, using a ligase that can ligate the 3′ end of RNA to the 5′ end of DNA using a DNA template, such as T4 DNA ligase. Following digestion to form an elongated target polynucleotide with a liberated end, a reverse transcription reaction can be performed, followed by a PCR. In some embodiments, the methods of the present teachings can discriminate between polymorphic polynucleotides that vary by as little as one nucleotide.

    摘要翻译： 本教导提供了用于量化靶标多核苷酸的方法，组合物和试剂盒。 在一些实施方案中，使用连接酶将反向茎环结合探针连接到靶多核苷酸的3'末端，该连接酶可以使用DNA模板如T4连接DNA的3'末端至DNA的5'末端 DNA连接酶。 在消化形成具有释放末端的细长靶多核苷酸之后，可以进行逆转录反应，随后进行PCR。 在一些实施方案中，本教导的方法可以区分多至一个核苷酸变化的多态性多核苷酸。

    Nek1-related protein kinase
    6.
    发明授权
    Nek1-related protein kinase 失效
    Nek1相关蛋白激酶

    公开(公告)号：US06713060B1

    公开(公告)日：2004-03-30

    申请号：US09481275

    申请日：2000-01-11

    申请人： Olga Bandman ,  Neil C. Corley ,  Karl J. Guegler ,  Mariah Baughn

    发明人： Olga Bandman ,  Neil C. Corley ,  Karl J. Guegler ,  Mariah Baughn

    IPC分类号： A61K39395

    CPC分类号： C12N9/1205 ,  A61K38/00

    摘要： The invention provides a human Nek1-related protein kinase (NRPK) and polynucleotides which identify and encode NRPK. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of NRPK.

    摘要翻译： 本发明提供了人Nek1相关蛋白激酶（NRPK）和识别和编码NRPK的多核苷酸。 本发明还提供表达载体，宿主细胞，抗体，激动剂和拮抗剂。 本发明还提供了用于诊断，治疗或预防与NRPK表达相关的紊乱的方法。

    Antibodies to a chemokine expressed in inflamed adenoid
    7.
    发明授权
    Antibodies to a chemokine expressed in inflamed adenoid 失效
    在发炎腺样体中表达的趋化因子的抗体

    公开(公告)号：US06692920B1

    公开(公告)日：2004-02-17

    申请号：US09588044

    申请日：2000-06-05

    申请人： Karl J. Guegler ,  Phillip R. Hawkins ,  Craig G. Wilde ,  Jeffrey J. Seilhamer

    发明人： Karl J. Guegler ,  Phillip R. Hawkins ,  Craig G. Wilde ,  Jeffrey J. Seilhamer

    IPC分类号： A61K39395

    CPC分类号： C07K14/522 ,  A61K38/00 ,  C12Q1/6883 ,  C12Q2600/158

    摘要： The present invention provides nucleotide and amino acid sequences that identify and encode a novel expressed chemokine (ADEC) from inflamed adenoid tissue. The present invention also provides for antisense molecules to the nucleotide sequences which encode ADEC, expression vectors for the production of purified ADEC, antibodies capable of binding specifically to ADEC, hybridization probes or oligonucleotides for the detection of ADEC-encoding nucleotide sequences, genetically engineered host cells for the expression of ADEC, diagnostic tests for inflammation or disease based on ADEC-encoding nucleic acid molecules or antibodies capable of binding specifically to ADEC.

    摘要翻译： 本发明提供鉴定和编码来自发炎腺样体组织的新型表达趋化因子（ADEC）的核苷酸和氨基酸序列。 本发明还提供编码ADEC的核苷酸序列的反义分子，用于产生纯化的ADEC的表达载体，能够特异性结合ADEC的抗体，用于检测编码ADEC的核苷酸序列的杂交探针或寡核苷酸的遗传工程宿主 用于表达ADEC的细胞，基于编码ADEC的核酸分子或能够特异性结合ADEC的抗体的炎症或疾病的诊断测试。

    Human ena/VASP-like protein splice variant
    9.
    发明授权
    Human ena/VASP-like protein splice variant 失效
    人类ena / VASP样蛋白剪接变体

    公开(公告)号：US06645499B1

    公开(公告)日：2003-11-11

    申请号：US09387811

    申请日：1999-09-01

    申请人： Preeti Lal ,  Karl J. Guegler ,  Neil C. Corley

    发明人： Preeti Lal ,  Karl J. Guegler ,  Neil C. Corley

    IPC分类号： A61K3900

    CPC分类号： C07K14/4702 ,  Y10S530/849 ,  Y10T436/143333

    摘要： The invention provides a human ena/VASP-like protein splice variant (EVL1) and polynucleotides which identify and encode EVL1. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of EVL1.

    摘要翻译： 本发明提供了鉴定和编码EVL1的人类ena / VASP样蛋白剪接变体（EVL1）和多核苷酸。 本发明还提供表达载体，宿主细胞，抗体，激动剂和拮抗剂。 本发明还提供了治疗或预防与EVL1表达相关的病症的方法。