公开(公告)号：US20100221768A1

公开(公告)日：2010-09-02

申请号：US12702526

申请日：2010-02-09

申请人： Tomonori Akai ,  Yasuhito Onishi ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Tamas Somfai

发明人： Tomonori Akai ,  Yasuhito Onishi ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Tamas Somfai

IPC分类号： C12Q1/02 ,  C12M1/22

CPC分类号： C12M23/12 ,  C12M23/10

摘要： An object of the present invention is to provide a culture dish appropriate for automatically identifying cultured cells. The present invention relates to a culture dish for culturing cells that require separate control, which has a bottom wall and a side wall, wherein cell-holding parts having wells are arranged on the bottom wall, 4 or more wells are adjacent to each other, the wall surface of each well has a concave surface that slopes upward from the lowest position to the outer edge of the well, and the pitch between adjacent wells is 1 mm or less.

摘要翻译： 本发明的目的是提供一种适于自动识别培养细胞的培养皿。 本发明涉及一种用于培养需要分开控制的细胞的培养皿，其具有底壁和侧壁，其中具有孔的细胞保持部件布置在底壁上，4个或更多个孔彼此相邻， 每个井的壁表面具有从井的最低位置向外倾斜向上的凹面，相邻孔之间的间距为1mm以下。

公开(公告)号：US20050164159A1

公开(公告)日：2005-07-28

申请号：US11038253

申请日：2005-01-21

申请人： Toshiaki Takezawa ,  Kei Imai ,  Toru Takahashi ,  Kazuyoshi Hashizume

发明人： Toshiaki Takezawa ,  Kei Imai ,  Toru Takahashi ,  Kazuyoshi Hashizume

IPC分类号： C12M3/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/073 ,  C12R1/91 ,  A01N1/02

CPC分类号： C12N5/0068 ,  C12N5/0604 ,  C12N2533/54

摘要： There is provided a carrier for co-culturing a fertilized ovum of an animal by which behavior of the fertilized ovum of an animal can be easily observed in a culture system and by which adhesion and three-dimensional growth of the fertilized ovum become possible at first, as well as there is provided a method of culturing the fertilized ovum of an animal using the co-culturing carrier. There is provided a carrier for co-culturing a fertilized ovum of an animal composed of a cell incorporated type three-dimensionally reconstructed tissue for co-culturing the fertilized ovum of an animal to induce adhesion and three-dimensional growth, of the fertilized ovum as well as there is provided a method of culturing the fertilized ovum of an animal characterized in that the co-culturing carrier is introduced into a culture vessel to culture the fertilized ovum of an animal.

摘要翻译： 提供了用于共培养动物受精卵的载体，通过该载体可以容易地在培养系统中观察到动物的受精卵的行为，并且首先使受精卵的粘附和三维生长成为可能 ，并且提供了使用共培养载体培养动物的受精卵的方法。 提供了一种用于共培养动物受精卵的载体，该动物由细胞结合型三维重建组织组成，用于共培养动物的受精卵以诱导受精卵的粘附和三维生长作为 因为提供了培养动物的受精卵的方法，其特征在于将共培养载体引入培养容器中以培养动物的受精卵。

公开(公告)号：US09163274B2

公开(公告)日：2015-10-20

申请号：US13807346

申请日：2011-06-30

申请人： Tomonori Akai ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Satoshi Sugimura

发明人： Tomonori Akai ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Satoshi Sugimura

IPC分类号： C12Q1/04 ,  A61B17/435 ,  A01K67/027 ,  C12N5/073 ,  C12M3/00 ,  C12M1/34 ,  C12M1/36

CPC分类号： G06T7/0016 ,  A01K67/027 ,  A01K2227/101 ,  A61B17/435 ,  C12M21/06 ,  C12M41/46 ,  C12M41/48 ,  C12N5/0604 ,  C12Q1/04 ,  G01N33/4833 ,  G06T2207/10016 ,  G06T2207/30044

摘要： An object of the present invention is to provide a means for efficiently obtaining mammalian embryos having high conception rates. A first aspect of the present invention is a method for selecting a mammalian embryo prepared by in vitro culture from a fertilized egg, comprising a step of selecting an embryo using two or more of the following indicators: the time from fertilization to the completion of first cleavage; the morphology at a stage after first cleavage and before second cleavage; the morphology at a stage after third cleavage and before fourth cleavage; and the amount of oxygen consumed at the early blastocyst stage, the blastocyst stage, or the expanded blastocyst stage.

摘要翻译： 本发明的目的是提供一种有效地获得具有高受孕率的哺乳动物胚胎的方法。 本发明的第一方面是一种选择通过从受精卵进行体外培养而制备的哺乳动物胚胎的方法，其包括使用以下两个或更多个指标来选择胚胎的步骤：从受精到完成第一次的时间 裂解 在第一次切割后和第二次切割之前的阶段的形态; 在第三次切割后和第四次切割之前的阶段的形态; 以及早期胚泡期，胚泡期或扩大胚泡期消耗的氧量。

公开(公告)号：US08515143B2

公开(公告)日：2013-08-20

申请号：US12654823

申请日：2010-01-05

申请人： Yasuhito Oonishi ,  Sho Sanami ,  Kei Imai

发明人： Yasuhito Oonishi ,  Sho Sanami ,  Kei Imai

IPC分类号： G06K9/00

CPC分类号： G06K9/00147 ,  C12M21/06 ,  C12M41/46

摘要： An embryo quality evaluation assistance system including an image pickup unit for picking up an image of the embryo, and a computer that communicates data with the embryo observing apparatus to assist quality evaluation of the embryo. The computer includes a time-series image storing unit that stores a time-series image picked up by the embryo observing apparatus, an embryo image extracting unit that extracts an embryo image from the time-series image, and an active site extracting unit that compares an embryo image based on a first time-series image with an embryo image based on a second time-series image picked up before or after a predetermined time from a pickup time of the first time-series image, and extracts as an active site a set of pixels when the difference between pixel values of corresponding pixels of the first and second time-series images is larger than a predetermined threshold value.

摘要翻译： 包括用于拾取胚胎图像的图像拾取单元的胚胎质量评估辅助系统以及与胚观察装置进行数据通信的计算机，以辅助胚胎的质量评估。 计算机包括时间序列图像存储单元，其存储由胚胎观察装置拾取的时间序列图像，从时间序列图像提取胚胎图像的胚胎图像提取单元和比较 基于具有基于在从第一时间序列图像的拾取时间起的预定时间之前或之后拾取的第二时间序列图像的具有胚胎图像的第一时间序列图像的胚胎图像，并且提取作为活动位点a 当第一和第二时间序列图像的相应像素的像素值之间的差异大于预定阈值时，像素集合。

公开(公告)号：US20130116499A1

公开(公告)日：2013-05-09

申请号：US13807346

申请日：2011-06-30

申请人： Tomonori Akai ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Satoshi Sugimura

发明人： Tomonori Akai ,  Kei Imai ,  Yoshio Aikawa ,  Masaki Ohtake ,  Satoshi Sugimura

IPC分类号： C12Q1/04 ,  A61B17/435

CPC分类号： G06T7/0016 ,  A01K67/027 ,  A01K2227/101 ,  A61B17/435 ,  C12M21/06 ,  C12M41/46 ,  C12M41/48 ,  C12N5/0604 ,  C12Q1/04 ,  G01N33/4833 ,  G06T2207/10016 ,  G06T2207/30044

摘要： An object of the present invention is to provide a means for efficiently obtaining mammalian embryos having high conception rates. A first aspect of the present invention is a method for selecting a mammalian embryo prepared by in vitro culture from a fertilized egg, comprising a step of selecting an embryo using two or more of the following indicators: the time from fertilization to the completion of first cleavage; the morphology at a stage after first cleavage and before second cleavage; the morphology at a stage after third cleavage and before fourth cleavage; and the amount of oxygen consumed at the early blastocyst stage, the blastocyst stage, or the expanded blastocyst stage.

摘要翻译： 本发明的目的是提供一种有效地获得具有高受孕率的哺乳动物胚胎的方法。 本发明的第一方面是一种选择通过从受精卵进行体外培养而制备的哺乳动物胚胎的方法，其包括使用以下两个或更多个指标来选择胚胎的步骤：从受精到完成第一次的时间 裂解 在第一次切割后和第二次切割之前的阶段的形态; 在第三次切割后和第四次切割之前的阶段的形态; 以及早期胚泡期，胚泡期或扩大胚泡期消耗的氧量。

公开(公告)号：US20070109632A1

公开(公告)日：2007-05-17

申请号：US11598795

申请日：2006-11-14

申请人： Kei Imai ,  Tetsuo Nishidate

发明人： Kei Imai ,  Tetsuo Nishidate

IPC分类号： G02B21/00

CPC分类号： G11B7/22 ,  G11B7/1353 ,  G11B7/1381

摘要： There is provided an optical pickup apparatus whereby the phase of a grating can be adjusted sufficiently even while further reducing the number of parts. A plate spring portion 12b formed in a bent manner is provided in the front part of a cover 12. The plate spring portion is composed of an annular plate-like portion 12ba having an outside diameter approximately the same as the diameter of a grating 3 and formed with a through hole “h” for passage of laser beams through the center axis and a connecting portion 12bb for connecting the edge portion of the plate-like portion 12ba and the front end of the cover 12. Therefore, the connecting portion 12bb is to be deformed elastically by loading in the axial direction with respect to the plate-like portion 12ba to serve as a so-called plate spring. As for the plate spring portion 12b, the connecting portion 12bb is deformed elastically and the plate-like portion 12ba is in contact with the grating 3, whereby the elastic force of the connecting portion 12bb is applied to the grating 3 in the axial direction.

摘要翻译： 提供了一种光学拾取装置，由此即使在进一步减少部件数量的同时也可以充分调节光栅的相位。 在盖12的前部设有以弯曲方式形成的板簧部分12b。 板簧部分由具有与光栅3的直径大致相同的外径的环形板状部分12ba组成，并形成有用于使激光束穿过中心轴线的通孔“h”，并且连接 部分12bb用于连接板状部分12ba的边缘部分和盖12的前端。 因此，连接部分12bb将相对于板状部分12ba沿轴向加载而弹性变形，以用作所谓的板簧。 对于板簧部分12b，连接部分12bb弹性变形，并且板状部分12ba与光栅3接触，由此连接部分12bb的弹力被施加到光栅3上 轴向。

公开(公告)号：US20100195877A1

公开(公告)日：2010-08-05

申请号：US12654823

申请日：2010-01-05

申请人： Yasuhito Oonishi ,  Sho Sanami ,  Kei Imai

发明人： Yasuhito Oonishi ,  Sho Sanami ,  Kei Imai

IPC分类号： G06K9/00

CPC分类号： G06K9/00147 ,  C12M21/06 ,  C12M41/46

摘要： An embryo quality evaluation assistance system including an image pickup unit for picking up an image of the embryo, and a computer that communicates data with the embryo observing apparatus to assist quality evaluation of the embryo. The computer includes a time-series image storing unit that stores a time-series image picked up by the embryo observing apparatus, an embryo image extracting unit that extracts an embryo image from the time-series image, and an active site extracting unit that compares an embryo image based on a first time-series image with an embryo image based on a second time-series image picked up before or after a predetermined time from a pickup time of the first time-series image, and extracts as an active site a set of pixels when the difference between pixel values of corresponding pixels of the first and second time-series images is larger than a predetermined threshold value.

摘要翻译： 包括用于拾取胚胎图像的图像拾取单元的胚胎质量评估辅助系统以及与胚观察装置进行数据通信的计算机，以辅助胚胎的质量评估。 计算机包括时间序列图像存储单元，其存储由胚胎观察装置拾取的时间序列图像，从时间序列图像提取胚胎图像的胚胎图像提取单元和比较 基于具有基于在从第一时间序列图像的拾取时间起的预定时间之前或之后拾取的第二时间序列图像的具有胚胎图像的第一时间序列图像的胚胎图像，并且提取作为活动位点a 当第一和第二时间序列图像的相应像素的像素值之间的差异大于预定阈值时，像素集合。

公开(公告)号：US20060192061A1

公开(公告)日：2006-08-31

申请号：US11362038

申请日：2006-02-27

申请人： Kei Imai ,  Tetsuo Nishidate ,  Masaaki Suetsugu

发明人： Kei Imai ,  Tetsuo Nishidate ,  Masaaki Suetsugu

IPC分类号： A47B96/06

CPC分类号： G11B7/22

摘要： A fitting structure for fitting a laser holder holding a semiconductor laser to a base member in an optical pickup device includes a support member of a metal plate that supports the laser holder. The support member integrally has: a base part having a laser passage hole that is formed in the center thereof for permitting laser light from the semiconductor laser to pass therethrough; an upper projecting part and a lower projecting part that respectively project from the upper and the lower edges of the base part so as to oppose each other and that support the laser holder so that the laser holder can swing about the vertical axis; and a bending part that is elastically deformable and that first projects from the upper edge of the base part toward a side opposite to a side toward which the upper projecting part projects and then is bent downward and also inward toward the base part. The base part is inserted from the lower projecting part along the plate-like fitting part of the base member, whereby the plate-like fitting part becomes sandwiched between the bending part and the base part by an elastic restoring force of the bending part, and then, after optical axis adjustment of the semiconductor laser, the laser holder, the support member, and the base member are fixed to one another with an adhesive.

摘要翻译： 用于将保持半导体激光器的激光器保持器配合到光学拾取装置中的基底构件的装配结构包括支撑激光器保持器的金属板的支撑构件。 支撑构件一体地具有：基部，其具有形成在其中心的激光通孔，用于允许来自半导体激光器的激光通过; 上突出部分和下突出部分分别从基部的上边缘和下边缘突出以彼此相对，并且支撑激光器保持器，使得激光器保持器能够围绕竖直轴线摆动; 以及弯曲部，其可弹性变形，并且首先从所述基部的上边缘朝向与所述上突出部突出的一侧相反的一侧突出，然后向下弯折并且朝向所述基部向内突出。 基部部分沿着基部构件的板状嵌合部从下突出部分插入，由此弯曲部分的弯曲部分和基部之间通过弯曲部分的弹性恢复力夹住板状装配部分，并且 然后，在半导体激光器的光轴调整之后，用粘合剂将激光器保持器，支撑部件和基底部件彼此固定。