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公开(公告)号:US20070154914A1
公开(公告)日:2007-07-05
申请号:US11583606
申请日:2006-10-18
申请人: David Gelfand , Keith Bauer , Amar Gupta , Veeraiah Bodepudi , John Niemiec
发明人: David Gelfand , Keith Bauer , Amar Gupta , Veeraiah Bodepudi , John Niemiec
CPC分类号: C12P19/34 , C12Q1/6827 , C12Q1/6848 , C12Q1/6886 , C12Q2600/156 , C12Q2565/301 , C12Q2525/186 , C12Q2521/101
摘要: The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.
摘要翻译: 本发明提供包含包含2'-终止子核苷酸的封闭寡核苷酸的反应混合物。 当从寡核苷酸中除去2'-终止子核苷酸时,例如通过焦磷酸分解,封闭的寡核苷酸可以延伸。 反应混合物可用于许多其它应用中的各种核酸聚合和/或扩增测定中。 除了反应混合物之外,本发明还提供相关方法和反应混合物。
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公开(公告)号:US20050037398A1
公开(公告)日:2005-02-17
申请号:US10879493
申请日:2004-06-28
申请人: David Gelfand , Fred Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas Myers
发明人: David Gelfand , Fred Reichert , Veeraiah Bodepudi , Amar Gupta , Stephen Will , Thomas Myers
CPC分类号: C12Q1/6869 , Y10S435/975 , C12Q2535/101 , C12Q2525/101 , C12Q2525/186
摘要: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
摘要翻译: 本发明提供扩增引物核酸和测序靶核酸的方法。 所述方法包括使用2'-终止子核苷酸来实现链终止。 除了相关的反应混合物和试剂盒之外,本发明还提供计算机和计算机可读介质。
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公开(公告)号:US20050037991A1
公开(公告)日:2005-02-17
申请号:US10879494
申请日:2004-06-28
申请人: Veeraiah Bodepudi , Stephen Will , David Gelfand
发明人: Veeraiah Bodepudi , Stephen Will , David Gelfand
IPC分类号: A61K31/513 , A61K31/522 , A61K31/7072 , A61K31/7076 , C07H19/00 , C07H19/06 , C07H19/16 , C12Q1/68
CPC分类号: C07H21/04 , C07H19/00 , C07H19/16 , C12Q1/6869
摘要: The invention provides compositions the comprise nucleotides and/or nucleosides having blocking groups at 2′-positions of sugar moieties. Methods of synthesizing these nucleic acids are also provided.
摘要翻译: 本发明提供了包含在糖部分的2'-位具有封闭基团的核苷酸和/或核苷的组合物。 还提供了合成这些核酸的方法。
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公开(公告)号:US07890268B2
公开(公告)日:2011-02-15
申请号:US11304448
申请日:2005-12-14
申请人: Thomas Koch , Roderic Fuerst , Uwe Kobold , Dieter Heindl , Herbert von der Eltz , David Gelfand , Ivo Gut , Christoph Steinbeck
发明人: Thomas Koch , Roderic Fuerst , Uwe Kobold , Dieter Heindl , Herbert von der Eltz , David Gelfand , Ivo Gut , Christoph Steinbeck
CPC分类号: G06F19/18 , C12Q1/6872
摘要: The present invention is directed to an improved analysis procedure for the comparative sequencing of nucleic acids using multistage mass spectrometry. More precisely, the invention is directed to a method enabling the de-novo sequencing of nucleic acid molecules using multistage mass spectrometry.
摘要翻译: 本发明涉及使用多级质谱法进行核酸比较测序的改进的分析程序。 更准确地说,本发明涉及一种能够使用多级质谱法进行核酸分子的即时测序的方法。
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公开(公告)号:US20060257885A1
公开(公告)日:2006-11-16
申请号:US11203622
申请日:2005-08-11
申请人: David Gelfand , Pamela Holland , Randall Saiki , Robert Watson
发明人: David Gelfand , Pamela Holland , Randall Saiki , Robert Watson
CPC分类号: C12P19/34
摘要: A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.
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公开(公告)号:US20060084092A1
公开(公告)日:2006-04-20
申请号:US11203048
申请日:2005-08-11
申请人: David Gelfand , Pamela Holland , Randall Saiki , Robert Watson
发明人: David Gelfand , Pamela Holland , Randall Saiki , Robert Watson
CPC分类号: C12P19/34
摘要: A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
摘要翻译: 使用标记的寡核苷酸检测靶核酸的过程使用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火标记的寡核苷酸,并释放标记的寡核苷酸片段进行检测。 该方法容易地并入PCR扩增测定中。
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公开(公告)号:US20060078914A1
公开(公告)日:2006-04-13
申请号:US11203040
申请日:2005-08-11
申请人: David Gelfand , Pamela Holland , Randall Saiki , Robert Watson
发明人: David Gelfand , Pamela Holland , Randall Saiki , Robert Watson
CPC分类号: C12P19/34
摘要: A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.
摘要翻译: 使用标记的寡核苷酸检测靶核酸的过程使用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火标记的寡核苷酸,并释放标记的寡核苷酸片段进行检测。 该方法容易地并入PCR扩增测定中。
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公开(公告)号:US20050255499A1
公开(公告)日:2005-11-17
申请号:US11043099
申请日:2005-01-27
申请人: David Gelfand , Pamela Holland , Randall Saiki , Robert Watson
发明人: David Gelfand , Pamela Holland , Randall Saiki , Robert Watson
CPC分类号: C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815 , C12Q2521/101 , C12Q2565/1015 , C12Q2531/113 , C12Q2521/319 , C12Q2535/131 , C12Q2521/325 , C12Q2537/157 , C12Q2561/101 , C12Q2525/186 , C12Q2525/131
摘要: A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
摘要翻译: 使用标记的寡核苷酸检测靶核酸的过程使用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火标记的寡核苷酸,并释放标记的寡核苷酸片段进行检测。 该方法容易地并入PCR扩增测定中。
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