摘要:
The present invention makes it possible to efficiently recognize carbon nanotubes, carbon nanohorns or modifiers thereof and to support functional compounds by fusing the ability of ferritin molecules capable of forming nanoparticles of inorganic metal atoms or inorganic metal compounds. In addition, because ferritin molecules are capable of forming two-dimensional crystals at the interface, the present invention makes it possible to align carbon nanotubes, carbon nanohorns with the use of the molecular arrangement ability of ferritin fused with nanographite structure recognition peptides. A nanographite structure/metal nanoparticle composite is constructed, wherein a nanoparticle of an inorganic metal atom or an inorganic metal compound is retained in an interior space of a protein in which a nanographite structure recognition peptide is fused or chemically bound to a surface of a cage protein such as ferritin, and wherein a plurality of nanoparticles of an inorganic metal atom or an inorganic metal compound are supported on a nanographite structure with the use of affinity of the nanographite structure recognition peptide to the nanographite structure.
摘要:
A method for selectively arranging ferritin modified with a peptide, which specifically binds to titanium, to titanium formed on a substrate surface is provided.The method for arranging ferritin of the present invention is characterized in that ferritin is selectively bound on titanium on a substrate by modifying the N-terminal part of ferritin with a peptide which specifically binds to titanium. Also, the method for arranging ferritin of the present invention is characterized in that selectivity for titanium can be markedly improved by adding a nonionic surface activating agent.
摘要:
Isolated, recombinant nucleic acids which encode alanyl-tRNA synthetase (AlaRS) of human origin have been used to make expression constructs and transformed host cells for the production of recombinant human AlaRS. The recombinant enzyme has been purified, and is active in the specific aminoacylation of tRNA by alanine. The isolated, recombinant human AlaRS is also recognized by antibodies made by patients with the particular autoimmune disease known as "antisynthetase syndrome" in which the patients produce antibodies against the human alanyl-tRNA synthetase in their own cells. Thus, the isolated, recombinant enzyme, and antibodies made specifically thereto, can be useful in assays to diagnose and monitor this disease. The essential alanyl-tRNA synthetases of microbes pathogenic in humans can be the targets of inhibitory agents having antimicrobial activity. The human alanyl-tRNA synthetase, isolated and purified, can be used to assess the toxic effect in humans of such an inhibitory agent in various biochemical activity assays. This human enzyme can also be expressed in "tester strains," whose cells rely upon the function of the human alanyl-tRNA synthetase for tRNA.sup.Ala charging. Such tester strains can be used to test for any toxic effects of an antimicrobial agent that specifically interacts with the heterologous human AlaRS gene or gene product.
摘要:
It is to provide an inorganic thin film of titanium dioxide or the like which is controlled at the nanoscale and a three-dimensional structure of a functional material such as semiconductor nanoparticles. A three-dimensional structure of an inorganic material is manufactured by introducing onto a surface of an inorganic substrate ferritin presenting on its surface a plurality of inorganic material-binding peptides; binding the ferritin in a monolayer onto the inorganic substrate; introducing an inorganic material onto the ferritin which is bound in a monolayer, while the inorganic material-binding peptides is having a binding and/or biomineralization ability for the inorganic material; forming a biomineral layer utilizing the biomineralization ability of the inorganic material-binding peptides; and subsequently repeating one or more times the steps (a) and (b) of a multilayering operation: (a) introducing onto the biomineral layer thus formed the ferritin having a binding ability to the biomineral layer, and binding the ferritin in a monolayer onto the biomineral layer; (b) introducing the inorganic material onto the surface of the ferritin which is bound in a monolayer, and forming a biomineral layer.
摘要:
The present invention makes it possible to efficiently recognize carbon nanotubes, carbon nanohorns or modifiers thereof and to support functional compounds by fusing the ability of ferritin molecules capable of forming nanoparticles of inorganic metal atoms or inorganic metal compounds. In addition, because ferritin molecules are capable of forming two-dimensional crystals at the interface, the present invention makes it possible to align carbon nanotubes, carbon nanohorns with the use of the molecular arrangement ability of ferritin fused with nanographite structure recognition peptides. A nanographite structure/metal nanoparticle composite is constructed, wherein a nanoparticle of an inorganic metal atom or an inorganic metal compound is retained in an interior space of a protein in which a nanographite structure recognition peptide is fused or chemically bound to a surface of a cage protein such as ferritin, and wherein a plurality of nanoparticles of an inorganic metal atom or an inorganic metal compound are supported on a nanographite structure with the use of affinity of the nanographite structure recognition peptide to the nanographite structure.
摘要:
It is to provide an inorganic thin film of titanium dioxide or the like which is controlled at the nanoscale and a three-dimensional structure of a functional material such as semiconductor nanoparticles. A three-dimensional structure of an inorganic material is manufactured by introducing onto a surface of an inorganic substrate ferritin presenting on its surface a plurality of inorganic material-binding peptides; binding the ferritin in a monolayer onto the inorganic substrate; introducing an inorganic material onto the ferritin which is bound in a monolayer, while the inorganic material-binding peptides is having a binding and/or biomineralization ability for the inorganic material; forming a biomineral layer utilizing the biomineralization ability of the inorganic material-binding peptides; and subsequently repeating one or more times the steps (a) and (b) of a multilayering operation: (a) introducing onto the biomineral layer thus formed the ferritin having a binding ability to the biomineral layer, and binding the ferritin in a monolayer onto the biomineral layer; (b) introducing the inorganic material onto the surface of the ferritin which is bound in a monolayer, and forming a biomineral layer.
摘要:
It is intended to provide a peptide or a phage recognizing nanographite structures and thus enabling efficient recognition, binding, separation and alignment of nanographite structures such as carbon nanohorns or carbon nanotubes, an artificial protein or a chimeric molecule comprising the above-described peptide bonded to a functional peptide, a protein, a labeling, etc., and a complex of the above-described peptide molecule, artificial protein or chimeric molecule with a nanographite structure. By panning peptide-presenting phages bonded to nanographite structures, a nanographite structure-binding peptide capable of specifically recognizing nanographite structures such as carbon nanohorns or carbon nanotubes is obtained.
摘要:
The present invention provides a carbon nanohorn complex that is excellent in characteristics of adsorption or inclusion of drugs and release, in particular, a sustained release of drugs as a novel drug carrier in drug delivery systems, as well as a process for producing the complex. The complex of drug and carbon nanohorns comprises a steroidal or metal-containing drug being adsorbed onto the oxidized carbon nanohorns, or included in pores opened thereof.
摘要:
A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine to which a label capable of generating a detectable signal or a precursor thereof is bound, to form a complex between said nucleic acid and said polyamine, said precursor converting into said label, if used, removing the polyamine which has not formed any complex before or after the conversion of said precursor and then detecting said label.
摘要:
A method for selectively arranging ferritin modified with a peptide, which specifically binds to titanium, to titanium formed on a substrate surface is provided. The method for arranging ferritin of the present invention is characterized in that ferritin is selectively bound on titanium on a substrate by modifying the N-terminal part of ferritin with a peptide which specifically binds to titanium. Also, the method for arranging ferritin of the present invention is characterized in that selectivity for titanium can be markedly improved by adding a nonionic surface activating agent.