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公开(公告)号:US20100323439A1
公开(公告)日:2010-12-23
申请号:US12854465
申请日:2010-08-11
IPC分类号: C12N5/071
CPC分类号: C12N5/0075 , B01L3/5027 , B01L3/5085 , C12M23/12 , C12M23/16 , C12M23/34
摘要: Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.
摘要翻译: 公开了用于细胞培养的微流体装置及其使用方法。 一个装置包括基底和膜。 衬底包括与通道流体连通的储存器。 可以将生物相容的流体添加到储存器和通道中。 储存器被配置为接收和保留细胞块的至少一部分。 膜作为生物相容性流体从通道蒸发的障碍。 可以加入覆盖流体以覆盖生物相容性流体以防止生物相容性流体的蒸发。
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公开(公告)号:US20100323447A1
公开(公告)日:2010-12-23
申请号:US12854459
申请日:2010-08-11
申请人: Shuichi Takayama , Lourdes Marcella Cabrera , Nobuyuki Futai , Yun Seok Heo , Gary Daniel Smith , Xiaoyue Zhu
发明人: Shuichi Takayama , Lourdes Marcella Cabrera , Nobuyuki Futai , Yun Seok Heo , Gary Daniel Smith , Xiaoyue Zhu
IPC分类号: C12N5/07
CPC分类号: B01L3/502738 , B01L3/502715 , B01L3/50273 , B01L2300/0816 , B01L2300/0819 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , F04B19/006 , F04B43/043 , F16K99/0001 , F16K99/0026
摘要: Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.
摘要翻译: 公开了用于细胞培养的微流体装置及其使用方法。 一个装置包括基底和膜。 衬底包括与通道流体连通的储存器。 可以将生物相容的流体添加到储存器和通道中。 储存器被配置为接收和保留细胞块的至少一部分。 膜作为生物相容性流体从通道蒸发的障碍。 可以加入覆盖流体以覆盖生物相容性流体以防止生物相容性流体的蒸发。
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公开(公告)号:US20100247384A1
公开(公告)日:2010-09-30
申请号:US11582259
申请日:2006-10-17
申请人: Shuichi Takayama , Lourdes Marcella Cabrera , Nobuyuki Futai , Yun Seok Heo , Gary Daniel Smith , Xiaoyue Zhu
发明人: Shuichi Takayama , Lourdes Marcella Cabrera , Nobuyuki Futai , Yun Seok Heo , Gary Daniel Smith , Xiaoyue Zhu
IPC分类号: B01L3/00
CPC分类号: B01L3/502738 , B01L3/502715 , B01L3/50273 , B01L2300/0816 , B01L2300/0819 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , F04B19/006 , F04B43/043 , F16K99/0001 , F16K99/0026
摘要: Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.
摘要翻译: 公开了用于细胞培养的微流体装置及其使用方法。 一个装置包括基底和膜。 衬底包括与通道流体连通的储存器。 可以将生物相容的流体添加到储存器和通道中。 储存器被配置为接收和保留细胞块的至少一部分。 膜作为生物相容性流体从通道蒸发的障碍。 可以加入覆盖流体以覆盖生物相容性流体以防止生物相容性流体的蒸发。
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公开(公告)号:US08865464B2
公开(公告)日:2014-10-21
申请号:US12854465
申请日:2010-08-11
CPC分类号: C12N5/0075 , B01L3/5027 , B01L3/5085 , C12M23/12 , C12M23/16 , C12M23/34
摘要: Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.
摘要翻译: 公开了用于细胞培养的微流体装置及其使用方法。 一个装置包括基底和膜。 衬底包括与通道流体连通的储存器。 可以将生物相容的流体添加到储存器和通道中。 储存器被配置为接收和保留细胞块的至少一部分。 膜作为生物相容性流体从通道蒸发的障碍。 可以加入覆盖流体以覆盖生物相容性流体以防止生物相容性流体的蒸发。
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