利索-全球专利检索

  1. Login
  2. Sign Up

Search Results

52 records (took 0.039 seconds)

    Adenoviral expression vector and methods and cell lines for production
    4.
    发明授权
    Adenoviral expression vector and methods and cell lines for production 有权

    公开(公告)号：US12227754B2

    公开(公告)日：2025-02-18

    申请号：US17223785

    申请日：2021-04-06

    Applicant: Lung Biotechnology PBC

    Inventor： Colin Exline ,  Huizhu Liu ,  Sean Stevens

    IPC: C12N15/86 ,  C07K14/54 ,  C12N15/10

    Abstract: An adenovirus expression vector is provided. The adenovirus expression vector may include: a) one or more mutations that render the adenovirus replication incompetent and b) at least one nucleotide sequence encoding a protein or an RNA is provided. A method of synthesizing an adenovirus vector is also provided. The synthesis may include: a) producing a plurality of overlapping adenovirus sub-fragments, each sub-fragment comprising a portion of the full genome of the adenovirus; b) circularizing the sub-fragments to form plasmid structures; and c) assembling the circularized sub-fragments into a linear structure, wherein the vector comprises a combination of two or more sub-fragments. A mammalian cell line configured to replicate adenoviral vectors, wherein the cell line comprises nucleotide sequences expressing E1A and E1B gene products but is devoid of other adenovirus sequences is also provided.

    CCL20 PROMOTER-REGULATED GENE EXPRESSION
    5.
    发明公开
    CCL20 PROMOTER-REGULATED GENE EXPRESSION 审中-公开

    公开(公告)号：US20240350675A1

    公开(公告)日：2024-10-24

    申请号：US18611465

    申请日：2024-03-20

    Applicant: Lung Biotechnology PBC

    Inventor： Colin EXLINE

    IPC: A61K48/00 ,  A61K38/00 ,  A61P29/00 ,  A61P37/06 ,  C07K14/545 ,  C12N15/86 ,  G01N33/68

    CPC classification number: A61K48/0066 ,  A61P29/00 ,  A61P37/06 ,  C07K14/545 ,  C12N15/86 ,  G01N33/6863 ,  A61K38/00 ,  C12N2710/16143

    Abstract: The present disclosure relates to CCL20 promoters derived from the regulatory region of the CCL20 gene that stimulate gene expression in response to inflammation. These CCL20 promoters, which can be stimulated by an endogenous or exogenous cytokine, can be used to control the timing of the expression of a heterologous gene. The present disclosure also relates to expression cassettes or vectors comprising the CCL20 promoters of the present disclosure operably linked to a nucleic acid sequence encoding, e.g., a polypeptide of interest, as well as delivery systems (e.g., viral particles, lipid vesicles, or nanoparticles) or cells comprising such expression cassettes or vectors. The present disclosure also relates to the use of the promoters, expression cassettes, vectors, delivery systems, or cells in the treatment of diseases related to inflammation, or for recombinant gene expression.

Patent Agency Ranking