公开(公告)号：US5374539A

公开(公告)日：1994-12-20

申请号：US716091

申请日：1991-06-17

申请人： Marcel E. Nimni ,  David T. Cheung

发明人： Marcel E. Nimni ,  David T. Cheung

IPC分类号： A61L27/36 ,  C08H1/06 ,  A61K31/78 ,  A61B17/00 ,  C09H1/04

CPC分类号： A61L27/3683 ,  C08H1/06 ,  A61L2430/40

摘要： A process is described to purify collagen fibers while allowing the individual constituent molecules to retain their native configuration and 3-dimensional arrangement characteristic of the tissues from which they are derived. Collagenous tissues used as sources of purified collagen and for manufacturing bioprosthesis contain significant amounts of other substances (elastin, glycoproteins, polysaccharides, cell derived materials, etc.). This process becomes therefore useful to selectively preserve the collagen in its native conformation and to eliminate contaminants. The method described allows for enzymatic removal of all extraneous materials while preserving the native collagen molecules in their original fiber configuration. Subsequently this network of native collagen fibrils can be crosslinked using standard bifunctional crosslinking reagents, treated with other chemical substances, physical methods of insolubilization, or can be disrupted chemically or mechanically into smaller molecular aggregates or into the constituent single molecules.

摘要翻译： 描述了一种纯化胶原纤维的方法，同时允许各个组分分子保持其天然构型和从其衍生的组织的3维排列特征。 用作纯化胶原的来源和用于制造生物假体的胶原组织含有大量的其他物质（弹性蛋白，糖蛋白，多糖，细胞来源的材料等）。 因此，该方法可用于选择性地保留其天然构象中的胶原蛋白并消除污染物。 所描述的方法允许酶除去所有外来材料，同时保留其原始纤维构型中的天然胶原分子。 随后，这种天然胶原原纤维网可以使用标准的双功能交联剂交联，用其它化学物质处理，不溶解的物理方法，或者可以化学或机械地破坏成较小分子聚集体或构成单分子。

公开(公告)号：US07357947B2

公开(公告)日：2008-04-15

申请号：US10413171

申请日：2003-04-11

申请人： Marcel E. Nimni

发明人： Marcel E. Nimni

IPC分类号： A61K9/14 ,  A61K35/32 ,  A61F2/00

CPC分类号： A61K35/32

摘要： One embodiment of the invention is demineralized bone putty composition comprises: (1) demineralized bone matrix (DBM); and (2) a lipid fraction selected from the group consisting of lecithin and a mixture of lecithin and triglycerides containing unsaturated fatty acids. The putty composition is moldable, biocompatible, slowly resorbable, insoluble in tissue fluids, and non-extrudable. The composition delivers a biologically active product to animals and humans that will enhance bone formation at sites where bone is lost, deficient, or present in suboptimal amounts. The composition can further comprise calcium, an antioxidant such as Vitamin E or Vitamin C, or a hydrophilic polymer such as methylcellulose, a methylcellulose derivative, carboxymethyl cellulose, or hydroxypropyl methylcellulose. A second embodiment of the invention is a demineralized bone paste composition comprising: (1) about 15% to about 75% of an emulsion carrier, such as an aqueous phase; and (2) a bone-material-containing phase comprising: (a) demineralized bone matrix (DBM); and (b) an emulsifier component that is compatible with lipids. This bone paste composition is moldable, biocompatible, slowly resorbable, miscible with bone graft materials, soluble or partially soluble in tissue fluids, and extrudable.

摘要翻译： 本发明的一个实施方案是软化骨灰质组合物，包括：（1）软化骨基质（DBM）; 和（2）选自卵磷脂和含有不饱和脂肪酸的卵磷脂和甘油三酯的混合物的脂质部分。 腻子组合物是可模塑的，生物相容的，缓慢吸收的，不溶于组织液，不可挤出。 该组合物向动物和人类递送生物活性产品，其将在骨丢失，缺陷或以不理想的量存在的部位增强骨形成。 该组合物可进一步包含钙，抗氧化剂如维生素E或维生素C，或亲水性聚合物如甲基纤维素，甲基纤维素衍生物，羧甲基纤维素或羟丙基甲基纤维素。 本发明的第二个实施方案是一种软化骨糊组合物，其包含：（1）约15％至约75％的乳液载体，例如水相; 和（2）含骨材料的相，包含：（a）软化骨基质（DBM）; 和（b）与脂质相容的乳化剂组分。 该骨糊组合物是可模塑的，生物相容的，缓慢吸收的，与骨移植材料混溶，可溶或部分可溶于组织液，并可挤出。

公开(公告)号：US4820724A

公开(公告)日：1989-04-11

申请号：US846171

申请日：1986-03-31

申请人： Marcel E. Nimni

发明人： Marcel E. Nimni

IPC分类号： A61K31/27 ,  A61K9/00 ,  A61K9/08 ,  A61K31/34 ,  A61K31/415 ,  A61K31/425 ,  A61K45/08 ,  A61K47/00 ,  A61K47/10 ,  A61P31/04 ,  A61K31/12 ,  A61K31/21

CPC分类号： A61K47/10 ,  A61K9/0014 ,  Y10S514/858 ,  Y10S514/947

摘要： A dual phase solvent carrier system for topically applying at least one pharmaceutically active compound comprised of the active compound dissolved in at least one delivery solvent and at least one fugitive solvent, with a particularly useful composition for topically treating dermatophytic infections comprised of of griseofulvin, benzyl alcohol and at least one fugitive solvent.

摘要翻译： 一种用于局部施用至少一种药物活性化合物的双相溶剂载体体系，所述药物活性化合物包含溶于至少一种递送溶剂中的活性化合物和至少一种缓冲溶剂，以及特别有用的组合物，用于局部治疗皮肤灰质炎感染，包括灰黄霉素，苄基 酒精和至少一种挥发性溶剂。

公开(公告)号：US20120010146A1

公开(公告)日：2012-01-12

申请号：US13127275

申请日：2009-11-03

申请人： Bo Han ,  Marcel E. Nimni

发明人： Bo Han ,  Marcel E. Nimni

IPC分类号： A61K38/39 ,  A61P17/00

CPC分类号： A61L27/227 ,  A61K8/64 ,  A61K38/39 ,  A61K2800/91 ,  A61L2400/06 ,  A61Q19/00

摘要： An injectable composition having dermal filling and tissue expanding activity comprises (1) a quantity of elastin sufficient to bring about dermal filling and tissue expansion when injected into a subject in need of dermal filling and tissue expansion, and (2) a pharmaceutically acceptable carrier. The composition can further comprise collagen, in other alternatives, the composition can further comprise hyaluronic acid, and one or more of the elastin, the collagen, and the hyaluronic acid, if present can be cross-linked, either intramolecularly or intermolecularly. The elastin, however, is the primary filler, even if collagen or hyaluronic acid are included in the composition

摘要翻译： 具有皮肤填充和组织扩张活性的可注射组合物包括（1）当注射到需要皮肤填充和组织扩张的受试者中时，足以引起皮肤填充和组织扩张的弹性蛋白量，和（2）药学上可接受的载体。 组合物可以进一步包含胶原，在其它替代方案中，组合物还可以包含透明质酸，并且弹性蛋白，胶原和透明质酸中的一种或多种（如果存在的话）可以在分子内或分子内交联。 然而，弹性蛋白是主要的填充剂，即使胶原蛋白或透明质酸被包括在组合物中

公开(公告)号：US06565884B2

公开(公告)日：2003-05-20

申请号：US09950264

申请日：2001-09-10

申请人： Marcel E. Nimni

发明人： Marcel E. Nimni

IPC分类号： A61K914

CPC分类号： A61K35/32

摘要： A demineralized bone putty composition comprises: (1) demineralized bone matrix (DBM); and (2) a lipid fraction selected from the group consisting of lecithin and a mixture of lecithin and triglycerides containing unsaturated fatty acids. The putty composition is moldable, biocompatible, slowly resorbable, and soluble in tissue fluids, and non-extrudable. The composition delivers a biologically active product to animals and humans that will enhance bone formation at sites where bone is lost, deficient, or present in suboptimal amounts. The composition can further comprise calcium, an antioxidant such as Vitamin E or Vitamin C, or a hydrophilic polymer such as methylcellulose or hydroxypropyl methylcellulose.

摘要翻译： 软骨骨灰组合物包括：（1）脱矿骨基质（DBM）; 和（2）选自卵磷脂和含有不饱和脂肪酸的卵磷脂和甘油三酯的混合物的脂质部分。 油灰组合物是可模塑的，生物相容的，缓慢吸收的，并且可溶于组织液，并且不可挤出。 该组合物向动物和人类递送生物活性产品，其将在骨丢失，缺陷或以不理想的量存在的部位增强骨形成。 该组合物可进一步包含钙，抗氧化剂如维生素E或维生素C，或亲水性聚合物如甲基纤维素或羟丙基甲基纤维素。

公开(公告)号：US06372794B1

公开(公告)日：2002-04-16

申请号：US09383845

申请日：1999-08-26

申请人： Marcel E. Nimni

发明人： Marcel E. Nimni

IPC分类号： A61K3800

CPC分类号： A61K38/39 ,  A61K31/726 ,  A61K31/737 ,  A61K47/61 ,  C08B37/0069 ,  C08H1/06 ,  Y10S514/801

摘要： A method for alleviating arthritis in mammals by the oral administration of a pharmaceutical composition compound of native Type II collagen in helical form and sulfated polysaccharides found in mammalian cartilage, the Type II collagen and sulfated polysaccharides being ionically bound.

摘要翻译： 通过口服施用螺旋形式的天然II型胶原的药物组合物化合物和在哺乳动物软骨中发现的硫酸化多糖，II型胶原和硫酸化多糖离子结合来减轻哺乳动物关节炎的方法。

公开(公告)号：US4772288A

公开(公告)日：1988-09-20

申请号：US62027

申请日：1987-06-15

申请人： William H. Borner ,  Marcel E. Nimni ,  Rudy C. Shepard

发明人： William H. Borner ,  Marcel E. Nimni ,  Rudy C. Shepard

IPC分类号： A61L27/36 ,  A61L17/00

CPC分类号： A61L27/3662 ,  A61L27/3683 ,  A61L2430/10 ,  Y10S623/917

摘要： A method of making implantable ligament and tendon prostheses from natural collagen-containing tissues is described. The tissues used are those wherein the collagen fibers are aligned in one direction such as in ligaments or tendons. The method comprises disrupting the interfibrillar matrix physically by mechanical means such as a roller or rollers in order to generate an expanded network of fibers that are more easily further treated chemically and which also generate a more favorable substrate for tissue ingrowth after implantation. Prostheses made from separated collagen fiber bundles which retain their natural configuration and length (as opposed to reconstituted collagen), exhibit improved softness and flexibility when compared with prostheses made from conventional chemically fixed (e.g., glutaraldehyde cross-linked) tendons which have not been separated as described herein. Another implementation of the developed new technology allows for the formation of composites between the dissociated collagen fibers involving two or more tissues or between collagen and synthetic fibers. These composites can also include absorbable materials.

摘要翻译： 描述了从含有天然胶原的组织制备可植入韧带和腱假体的方法。 使用的组织是其中胶原纤维在一个方向上对齐的组织，例如在韧带或腱中。 该方法包括通过诸如辊或辊之类的机械装置物理地干扰纤维素基质，以便产生更容易进一步化学处理的纤维的扩展网络，并且还可以在植入后产生用于组织向内生长的更有利的底物。 与从未分离的常规化学固定（例如戊二醛交联）腱制成的假体相比，保留其自然构型和长度（与复原胶原相反）的分离的胶原纤维束制成的假肢显示出改善的柔软性和柔韧性。 如本文所述。 开发的新技术的另一实施方式允许在涉及两个或更多个组织或胶原与合成纤维之间的解离的胶原纤维之间形成复合物。 这些复合材料也可以包括可吸收材料。

公开(公告)号：US5935994A

公开(公告)日：1999-08-10

申请号：US87594

申请日：1998-05-29

申请人： Marcel E. Nimni

发明人： Marcel E. Nimni

IPC分类号： A61K31/415 ,  A61K31/355 ,  A61K31/195 ,  A61K31/34

CPC分类号： A61K31/415

摘要： A composition and method for enhancing the appearance of the skin, the composition containing a mixture of essential amino acids, a penetrant, a neucleotide, vitamin C and vitamin E.

摘要翻译： 用于增强皮肤外观的组合物和方法，所述组合物含有必需氨基酸，渗透剂，新核苷酸，维生素C和维生素E的混合物。

公开(公告)号：US4378224A

公开(公告)日：1983-03-29

申请号：US188964

申请日：1980-09-19

申请人： Marcel E. Nimni ,  David T. Cheung

发明人： Marcel E. Nimni ,  David T. Cheung

IPC分类号： A61F2/00 ,  A61L2/18 ,  A61L27/24 ,  A61L27/36 ,  A61L33/00 ,  C14C3/16 ,  B23Q23/00 ,  C14C15/00

CPC分类号： A61L27/3687 ,  A61L2/18 ,  A61L27/24 ,  A61L33/0011 ,  A61F2310/00365 ,  A61L2400/02

摘要： A coating and integral treatment for improving the biophysical stability of bioprosthetic devices after implantation, particularly with respect to calcification, and, specifically, a method for treating animal tissues, such as heart valves, to provide improved biophysical stability in allograft and heterograft transplantations. The increased stability results, in-part, from the creation of a three-dimensional matrix of a primary structural component of the prosthetic device and covalently attached calcification inhibitors. Other materials, some having additional stabilizing effects, may be utilized to form additional bridges or fill the interstitial gaps in the matrix. After implantation, the resultant modified device exhibits minimal surface for intramatrix growth of calcium phosphate crystals, and additionally, may inhibit platelet aggregation, enzymatic degradation and host rejection, while minimizing the risk of mechanical failure, in the host organism.

摘要翻译： 用于改善植入后生物假体装置的生物物理稳定性的涂层和整体处理，特别是关于钙化，特别是用于治疗动物组织如心脏瓣膜的方法，以提供同种异体移植和异种移植移植中改善的生物物理稳定性。 增加的稳定性部分地产生由修复装置的主要结构组分的三维基质的产生和共价连接的钙化抑制剂。 可以利用其它具有额外的稳定作用的材料来形成额外的桥，或填充基体中的间隙。 植入后，所得到的修饰的装置表现出磷酸钙晶体肌内生长的最小表面，此外，可以抑制宿主生物体内的血小板聚集，酶降解和宿主排斥，同时最小化机械衰竭的风险。

公开(公告)号：US09248165B2

公开(公告)日：2016-02-02

申请号：US13127275

申请日：2009-11-03

申请人： Bo Han ,  Marcel E. Nimni

发明人： Bo Han ,  Marcel E. Nimni

IPC分类号： A61K38/39 ,  A61K8/64 ,  A61Q19/00

CPC分类号： A61L27/227 ,  A61K8/64 ,  A61K38/39 ,  A61K2800/91 ,  A61L2400/06 ,  A61Q19/00

摘要： An injectable composition having dermal filling and tissue expanding activity comprises (1) a quantity of elastin sufficient to bring about dermal filling and tissue expansion when injected into a subject in need of dermal filling and tissue expansion, and (2) a pharmaceutically acceptable carrier. The composition can further comprise collagen, in other alternatives, the composition can further comprise hyaluronic acid, and one or more of the elastin, the collagen, and the hyaluronic acid, if present can be cross-linked, either intramolecularly or intermolecularly. The elastin, however, is the primary filler, even if collagen or hyaluronic acid are included in the composition.

摘要翻译： 具有皮肤填充和组织扩张活性的可注射组合物包括（1）当注射到需要皮肤填充和组织扩张的受试者中时，足以引起皮肤填充和组织扩张的弹性蛋白量，和（2）药学上可接受的载体。 组合物可以进一步包含胶原，在其它替代方案中，组合物还可以包含透明质酸，并且弹性蛋白，胶原和透明质酸中的一种或多种（如果存在的话）可以在分子内或分子内交联。 然而，弹性蛋白是主要的填充剂，即使胶原蛋白或透明质酸被包括在组合物中。