公开(公告)号：US4876242A

公开(公告)日：1989-10-24

申请号：US99367

申请日：1987-09-21

申请人： Joy D. Applebaum ,  Marvin L. Bayne ,  Margaret A. Cascieri

发明人： Joy D. Applebaum ,  Marvin L. Bayne ,  Margaret A. Cascieri

IPC分类号： A23K1/165 ,  A61K38/00 ,  A61K38/27 ,  A61P7/06 ,  A61P15/00 ,  A61P43/00 ,  C07K14/00 ,  C07K14/575 ,  C07K14/62 ,  C07K14/65 ,  C12N15/09 ,  C12P21/02 ,  C12R1/19 ,  C12R1/865 ,  C12R1/91

CPC分类号： C07K14/65 ,  C07K14/62 ,  A61K38/00 ,  C07K2319/00

摘要： A synthetic gene encoding a 71-amino acid analog of human insulin-like grouwth factor (hIGF-I) has been constructed and expressed in the yeast, Saccharomyces cerevisiae. The protein analog, IGF132, contains the first 17 amino acids of the B chain of human insulin in place of the first 16 amino acids of hIGF-I. The purified hybrid protein has high affinity for the type I IGF recepto (12 nM) yet has drastically reduced affinity for human serum carrier proteins (>1000 nM). This analog is 5 to 10 times more active than normal hIGF-I in stimulating DNA synthesis in 3T3 cells and is a more active growth factor in vivo due to its reduced affinity for serum carrier proteins. Other proteins with similar properties have also been constructed. The protein analogs thus have a variety of utilities such as in promoting lactation in animals; promoting growth and feed efficiency in animals; improving carcass quality by increasing lean and decreasing fat; promoting wound healing in animals, including humans; promoting glucose utilization in skeletal muscle, and stimulating erythropoiesis, the production of red blood cells.

公开(公告)号：US06511827B1

公开(公告)日：2003-01-28

申请号：US09595549

申请日：2000-06-16

申请人： Andrew D. Howard ,  Margaret A. Cascieri ,  Roy G. Smith ,  Kathleen A. Sullivan ,  Carina Tan ,  Leonardus H. T. Van Der Ploeg ,  Kevin R. Lynch

发明人： Andrew D. Howard ,  Margaret A. Cascieri ,  Roy G. Smith ,  Kathleen A. Sullivan ,  Carina Tan ,  Leonardus H. T. Van Der Ploeg ,  Kevin R. Lynch

IPC分类号： C12N1512

CPC分类号： C07K14/72 ,  A61K38/00

摘要： A new galanin receptor, GALR3, is described. Also provided are nucleic acids encoding same and various assays to identify ligands particular to said receptor. Ligands so identified are useful for the treatment of obesity, treatment of pain, and treatment of cognitive disorders.

摘要翻译： 描述了一种新的甘丙肽受体GALR3。 还提供了编码相同的核酸和用于鉴定所述受体特异性的配体的各种测定法。 如此鉴定的配体可用于治疗肥胖症，治疗疼痛和治疗认知障碍。

公开(公告)号：US6001970A

公开(公告)日：1999-12-14

申请号：US817869

申请日：1997-05-06

申请人： Margaret A. Cascieri ,  Douglas John MacNeil ,  Catherine D. Strader

发明人： Margaret A. Cascieri ,  Douglas John MacNeil ,  Catherine D. Strader

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P3/08 ,  A61P3/10 ,  A61P9/00 ,  A61P9/02 ,  A61P25/00 ,  A61P25/28 ,  A61P43/00 ,  C07K14/705 ,  C07K14/72 ,  C12N5/10 ,  C12P21/02 ,  C12P21/04 ,  C12R1/91 ,  C07H21/04 ,  C12N15/11

CPC分类号： C07K14/723

摘要： Modified neuropeptide Y receptors having deletions, replacements or additions in the third intracellular domain are identified and methods of making the modified receptors are provided. The invention includes the modified receptors, assays employing the modified receptors, cells expressing the modified receptors, compounds identified through the use of the modified receptors, including modulators of the receptors, and the use of the compounds to treat conditions, including obesity, diabetes, anxiety, hypertension, cocaine withdrawal, congestive heart failure, memory enhancement, cardiac and cerebral vasospasm, pheochromocytoma and ganglioneuroblastoma, and Huntington's, Alzheimer's and Parkinson's diseases.

摘要翻译： PCT No.PCT / US95 / 14377 Sec。 371日期：1997年5月6日 102（e）日期1997年5月6日PCT提交1995年11月6日PCT公布。 公开号WO96 / 14331 日期1996年5月17日鉴定了在第三细胞内结构域中具有缺失，替换或添加的改良的神经肽Y受体，并提供了修饰受体的制备方法。 本发明包括修饰的受体，使用修饰的受体的测定，表达修饰的受体的细胞，通过使用修饰的受体鉴定的化合物，包括受体的调节剂，以及该化合物用于治疗包括肥胖，糖尿病， 焦虑，高血压，可卡因戒断，充血性心力衰竭，记忆增强，心脏和脑血管痉挛，嗜铬细胞瘤和神经节细胞瘤，亨廷顿舞蹈症，阿尔茨海默氏症和帕金森病。

公开(公告)号：US07029868B2

公开(公告)日：2006-04-18

申请号：US10204200

申请日：2001-02-13

申请人： Kathleen A. Sullivan ,  Diana Benincasa ,  Margaret A. Cascieri ,  Lyndon J. Mitnaul ,  Lin-Lin Shiao ,  Michael R. Tota

发明人： Kathleen A. Sullivan ,  Diana Benincasa ,  Margaret A. Cascieri ,  Lyndon J. Mitnaul ,  Lin-Lin Shiao ,  Michael R. Tota

IPC分类号： C12Q1/34 ,  C12Q1/00 ,  C12N5/00 ,  C12N15/02 ,  C07H19/04

CPC分类号： G01N33/505 ,  C12Q1/34 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5044 ,  G01N33/542 ,  G01N2333/986 ,  G01N2500/20

摘要： Methods of identifying inhibitors of the fusion of two types of cells, particularly when fusion is mediated by the interaction of a viral protein and such cellular proteins as CD4 and chemokine receptors, are disclosed. The methods are suitable for identifying substances that are useful for the treatment and prevention of viral diseases. Particularly preferred methods are useful for the identification of inhibitors of HIV-1 infection.

公开(公告)号：US5939263A

公开(公告)日：1999-08-17

申请号：US894236

申请日：1997-07-25

申请人： Margaret A. Cascieri ,  Douglas J. MacNeil ,  Lin-Lin Shiao ,  David H. Weinberg ,  Carina P. Tan ,  David L. Linemeyer ,  Catherine D. Strader

发明人： Margaret A. Cascieri ,  Douglas J. MacNeil ,  Lin-Lin Shiao ,  David H. Weinberg ,  Carina P. Tan ,  David L. Linemeyer ,  Catherine D. Strader

IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K14/705 ,  C07K16/28 ,  C12N5/10 ,  C12N15/09 ,  C12P21/02 ,  C12R1/91 ,  G01N33/15 ,  G01N33/74 ,  C12Q1/68 ,  C12N15/85 ,  C12Q1/02

CPC分类号： C07K14/70571 ,  G01N33/74 ,  A61K38/00 ,  C12N2799/026

摘要： A novel mammalian neuropeptide Y receptor and method of making the receptor are provided. The invention includes DNA encoding the receptor, the receptor, assays employing the receptor, cells expressing the receptor, antibodies which bind specifically to the receptor, RNA encoded by the DNA sequence or its complementary sequence, and single-stranded DNA with a sequence complementary to the RNA which encodes the receptor. The receptor and assays employing the receptor are useful for identifying compounds which bind to the receptor, including specific modulators of the receptor. Such compounds are useful for treating a variety of disease conditions, including obesity, diabetes, anxiety, hypertension, cocaine withdrawal, congestive heart failure, memory enhancement, cardiac and cerebral vasospasm, pheochromocytoma and ganglioneuroblastoma, and Huntington's, Alzheimer's and Parkinson's diseases.

摘要翻译： PCT No.PCT / US96 / 01444 Sec。 371日期1997年7月25日 102（e）日期1997年7月25日PCT 1996年1月30日PCT PCT。 第WO96 / 23809号公报 日期：1996年8月8日提供了一种新型哺乳动物神经肽Y受体及其制备方法。 本发明包括编码受体的DNA，受体，使用受体的测定，表达受体的细胞，与受体特异性结合的抗体，由DNA序列或其互补序列编码的RNA，以及与DNA序列互补的单链DNA 编码受体的RNA。 使用受体的受体和测定法可用于鉴定结合受体的化合物，包括受体的特异性调节剂。 这些化合物可用于治疗各种疾病状况，包括肥胖症，糖尿病，焦虑症，高血压，可卡因戒断，充血性心力衰竭，记忆增强，心脏和脑血管痉挛，嗜铬细胞瘤和神经节细胞瘤，亨廷顿舞蹈症，阿尔茨海默病和帕金森病。

公开(公告)号：US5908830A

公开(公告)日：1999-06-01

申请号：US961749

申请日：1997-10-30

申请人： Roy G. Smith ,  Margaret A. Cascieri ,  Euan MacIntyre ,  Douglas J. MacNeil ,  John G. Menke

发明人： Roy G. Smith ,  Margaret A. Cascieri ,  Euan MacIntyre ,  Douglas J. MacNeil ,  John G. Menke

IPC分类号： A61K31/24 ,  A61K38/22 ,  C07K14/575 ,  A61K38/00 ,  C07K5/00 ,  C07K16/00

CPC分类号： C07K14/5759 ,  A61K31/24 ,  A61K38/2264

摘要： The combination of a metabolic rate modifying agent (e.g., a .beta..sub.3 adrenergic receptor agonist) and a feeding behavior modifying agent (e.g., a NPY5 antagonist) is useful in the treatment of obesity and diabetes, either as compounds, pharmaceutically acceptable salts, pharmaceutical composition ingredients. Methods of treating obesity and diabetes are also described.

摘要翻译： 代谢速率调节剂（例如β3肾上腺素能受体激动剂）和进食行为改变剂（例如，NPY5拮抗剂）的组合可用于治疗肥胖症和糖尿病，作为化合物，药学上可接受的盐，药物 组成成分。 还描述了治疗肥胖和糖尿病的方法。

公开(公告)号：US5889167A

公开(公告)日：1999-03-30

申请号：US859873

申请日：1997-05-21

申请人： Margaret A. Cascieri ,  Gary A. Chicchi ,  Michael P. Graziano ,  Patricia J. Hey

发明人： Margaret A. Cascieri ,  Gary A. Chicchi ,  Michael P. Graziano ,  Patricia J. Hey

IPC分类号： C07K14/605 ,  C12N15/10 ,  C07K14/705 ,  C12N5/10

CPC分类号： C07K14/605

摘要： A synthetic human glucagon (hGlu) binding protein designated hGlu.DELTA.252-259 binding protein is cloned, expressed and used in an in vitro assay to screen for compounds that bind to the synthetic binding protein, including compounds that specifically stimulate or inhibit the binding of glucagon to the synthetic receptor. The invention includes the assay, the synthetic binding protein used in the assay, DNA encoding the synthetic binding protein, cells expressing the synthetic binding protein, and compounds identified through the use of the synthetic binding protein.

摘要翻译： 命名为hGlu DELTA 252-259结合蛋白的合成人胰高血糖素（hGlu）结合蛋白被克隆，表达并用于体外测定以筛选结合合成结合蛋白的化合物，包括特异性刺激或抑制结合蛋白的化合物 胰高血糖素合成受体。 本发明包括测定，测定中使用的合成结合蛋白，编码合成结合蛋白的DNA，表达合成结合蛋白的细胞，以及通过使用合成结合蛋白鉴定的化合物。

公开(公告)号：US5621079A

公开(公告)日：1997-04-15

申请号：US415818

申请日：1995-04-03

申请人： Margaret A. Cascieri ,  David L. Linemeyer ,  Douglas J. Macneil ,  Lin-Lin Shiao ,  Catherine D. Strader ,  David H. Weinberg ,  Carina P. Tan

发明人： Margaret A. Cascieri ,  David L. Linemeyer ,  Douglas J. Macneil ,  Lin-Lin Shiao ,  Catherine D. Strader ,  David H. Weinberg ,  Carina P. Tan

IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K14/705 ,  C07K16/28 ,  C12N5/10 ,  C12N15/09 ,  C12P21/02 ,  C12R1/91 ,  G01N33/15 ,  G01N33/74

CPC分类号： C07K14/70571 ,  G01N33/74 ,  A61K38/00 ,  C12N2799/026

摘要： A novel mammalian neuropeptide Y receptor and method of making the receptor are provided. The invention includes DNA encoding the receptor, the receptor, assays employing the receptor, cells expressing the receptor, antibodies which bind specifically to the receptor, RNA encoded by the DNA sequence or its complementary sequence, and single-stranded DNA with a sequence complementary to the RNA which encodes the receptor. The receptor and assays employing the receptor are useful for identifying compounds which bind to the receptor, including specific modulators of the receptor. Such compounds are useful for treating a variety of disease conditions, including obesity, diabetes, anxiety, hypertension, cocaine withdrawal, congestive heart failure, memory enhancement, cardiac and cerebral vasospasm, pheochromocytoma and ganglioneuroblastoma, and Huntington's, Alzheimer's and Parkinson's diseases.

摘要翻译： 提供了一种新型哺乳动物神经肽Y受体和制备受体的方法。 本发明包括编码受体的DNA，受体，使用受体的测定，表达受体的细胞，与受体特异性结合的抗体，由DNA序列或其互补序列编码的RNA，以及与DNA序列互补的单链DNA 编码受体的RNA。 使用受体的受体和测定法可用于鉴定结合受体的化合物，包括受体的特异性调节剂。 这些化合物可用于治疗各种疾病状况，包括肥胖症，糖尿病，焦虑症，高血压，可卡因戒断，充血性心力衰竭，记忆增强，心脏和脑血管痉挛，嗜铬细胞瘤和神经节细胞瘤，亨廷顿舞蹈症，阿尔茨海默病和帕金森病。