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公开(公告)号：US06348330B1
公开(公告)日：2002-02-19
申请号：US09482971
申请日：2000-01-12
IPC分类号： C07H2104
CPC分类号： C07K14/70571
摘要： A recombinant human neurokinin-3 receptor (hereinafter identified as human NK3R) is disclosed which has been prepared by polymerase chain reaction techniques. Also disclosed is the complete sequence of human NK3R complementary DNA; expression systems, including a CHO (chinese hamster ovarian cell line) stable expression system; and an assay using the CHO expression system. Human NK3R can be used in an assay to identify and evaluate entities that bind to the neurokinin-3 receptor.
摘要翻译： 公开了通过聚合酶链反应技术制备的重组人神经激肽-3受体（以下称为人NK3R）。 还公开了人NK3R互补DNA的完整序列; 表达系统，包括CHO（中国仓鼠卵巢细胞系）稳定表达系统; 和使用CHO表达系统的测定。 人NK3R可用于测定以鉴定和评估结合神经激肽-3受体的实体。
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公开(公告)号：US6001970A
公开(公告)日：1999-12-14
申请号：US817869
申请日：1997-05-06
IPC分类号： C12N15/09 , A61K38/00 , A61P3/08 , A61P3/10 , A61P9/00 , A61P9/02 , A61P25/00 , A61P25/28 , A61P43/00 , C07K14/705 , C07K14/72 , C12N5/10 , C12P21/02 , C12P21/04 , C12R1/91 , C07H21/04 , C12N15/11
CPC分类号： C07K14/723
摘要： Modified neuropeptide Y receptors having deletions, replacements or additions in the third intracellular domain are identified and methods of making the modified receptors are provided. The invention includes the modified receptors, assays employing the modified receptors, cells expressing the modified receptors, compounds identified through the use of the modified receptors, including modulators of the receptors, and the use of the compounds to treat conditions, including obesity, diabetes, anxiety, hypertension, cocaine withdrawal, congestive heart failure, memory enhancement, cardiac and cerebral vasospasm, pheochromocytoma and ganglioneuroblastoma, and Huntington's, Alzheimer's and Parkinson's diseases.
摘要翻译： PCT No.PCT / US95 / 14377 Sec。 371日期：1997年5月6日 102（e）日期1997年5月6日PCT提交1995年11月6日PCT公布。 公开号WO96 / 14331 日期1996年5月17日鉴定了在第三细胞内结构域中具有缺失，替换或添加的改良的神经肽Y受体，并提供了修饰受体的制备方法。 本发明包括修饰的受体，使用修饰的受体的测定，表达修饰的受体的细胞，通过使用修饰的受体鉴定的化合物，包括受体的调节剂，以及该化合物用于治疗包括肥胖，糖尿病， 焦虑，高血压，可卡因戒断，充血性心力衰竭，记忆增强，心脏和脑血管痉挛，嗜铬细胞瘤和神经节细胞瘤，亨廷顿舞蹈症，阿尔茨海默氏症和帕金森病。
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公开(公告)号：US5525712A
公开(公告)日：1996-06-11
申请号：US463488
申请日：1995-06-05
申请人： Tung M. Fong , Catherine D. Strader
发明人： Tung M. Fong , Catherine D. Strader
IPC分类号： C07K14/705 , C12Q1/68 , C07H21/04
CPC分类号： C07K14/705
摘要： Recombinant human neurokinin-1 receptor (human NKIR) is disclosed which has been isolated by polymerase chain reaction techniques. Also disclosed is the complete sequence of human NKIR cDNA, expression systems containing said isolated cDNA, including a CHO (Chinese Hamster Ovarian Cell Line) stable expression systems, and an assay using the CHO eexpression system. NKIR, also known as substance P receptor, can be used in an assay to identify and evaluate entities that bind substance P receptor. The assay can also be used in conjunction with diagnosis and therapy to determine the body fluid concnetration of substance P in arthritis patients.
摘要翻译： 公开了已经通过聚合酶链反应技术分离的重组人神经激肽-1受体（人NKIR）。 还公开了人NKIR cDNA的完整序列，含有所述分离的cDNA的表达系统，包括CHO（中国仓鼠卵巢细胞系）稳定表达系统，以及使用CHO表达系统的测定。 也称为物质P受体的NKIR可用于测定以鉴定和评估结合物质P受体的实体。 该测定法还可与诊断和治疗结合使用，以确定关节炎患者体内P物质的体液凝结。
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公开(公告)号：US5750826A
公开(公告)日：1998-05-12
申请号：US759848
申请日：1996-12-02
申请人： Joseph A. Borkowski , Howard Y. Chen , John W. Hess , Catherine D. Strader , Myrna E. Trumbauer
发明人： Joseph A. Borkowski , Howard Y. Chen , John W. Hess , Catherine D. Strader , Myrna E. Trumbauer
CPC分类号： C12N15/8509 , A01K67/0276 , A01K67/0278 , C07K14/72 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2217/072 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0356 , A01K2267/0368
摘要： A transgenic non-human animal with alterations in a bradykinin B2 receptor gene is prepared by introduction of a gene encoding an altered bradykinin B2 receptor into a host non-human animal.
摘要翻译： 通过将编码改变的缓激肽B2受体的基因导入宿主非人动物来制备具有缓激肽B2受体基因改变的转基因非人动物。
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公开(公告)号：US5484886A
公开(公告)日：1996-01-16
申请号：US117965
申请日：1993-09-07
申请人： Tung M. Fong , Catherine D. Strader
发明人： Tung M. Fong , Catherine D. Strader
IPC分类号： C07K14/705 , C12N15/12 , C07K14/435 , C07K14/71
CPC分类号： C07K14/705
摘要： Recombinant human neurokinin-1 receptor (hereinafter identified as human NK1R) has been prepared by polymerase chain reaction techniques. The complete sequence of human NK1R complementary DNA has been identified. Expression systems, including a CHO (chinese hamster ovarian cell line) stable expression system; and an assay using the CHO expression system have been developed. NK1R, also known as substance P receptor, may be used in an assay to identify and evaluate entities that bind to the substance P receptor. The assay may also be used in conjunction with diagnosis and therapy to determine the body fluid concentration of substance P antagonists in arthritis patients.
摘要翻译： 通过聚合酶链反应技术制备重组人神经激肽-1受体（以下称为人NK1R）。 已经鉴定了人NK1R互补DNA的完整序列。 表达系统，包括CHO（中国仓鼠卵巢细胞系）稳定表达系统; 并且已经开发了使用CHO表达系统的测定法。 也称为物质P受体的NK1R可用于测定以鉴定和评估结合物质P受体的实体。 该测定还可以与诊断和治疗结合使用以确定关节炎患者中物质P拮抗剂的体液浓度。
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公开(公告)号：US5336595A
公开(公告)日：1994-08-09
申请号：US701935
申请日：1991-05-17
申请人： Catherine D. Strader , Tung M. Fong
发明人： Catherine D. Strader , Tung M. Fong
IPC分类号： A61K38/00 , C07K14/715 , C12Q1/02 , G01N33/68 , C12Q1/68
CPC分类号： G01N33/68 , C07K14/715 , C12Q1/02 , G01N33/6872 , A61K38/00 , G01N2500/00
摘要： A novel recombinant human neurokinin-1 receptor short form (hereinafter identified as human NK1R sF) is disclosed which has been prepared by polymerase chain reaction techniques. Also disclosed is the complete sequence of human NK1R sF complementary DNA; expression systems, including a CHO (Chinese hamster ovarian cell line) stable expression system; and an assay using the CHO expression system.NK1R sF, can be used in an assay to identify and evaluate entities that bind substance P receptor or NK1R sF. The assay can also be used in conjunction with diagnosis and therapy to determine the body fluid concentration of substance P antagonists in arthritis patients.
摘要翻译： 公开了通过聚合酶链反应技术制备的新的重组人神经激肽-1受体短型（以下称为人NK1R sF）。 还公开了人NK1R sF互补DNA的完整序列; 表达系统，包括CHO（中国仓鼠卵巢细胞系）稳定表达系统; 和使用CHO表达系统的测定。 NK1R sF可用于测定以鉴定和评估结合物质P受体或NK1R sF的实体。 该测定法还可以与诊断和治疗结合使用，以确定关节炎患者中物质P拮抗剂的体液浓度。
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公开(公告)号：US06258943B1
公开(公告)日：2001-07-10
申请号：US08090369
申请日：1993-07-12
IPC分类号： C07K14705
CPC分类号： C07K14/70571
摘要： A recombinant human neurokinin-3 receptor (hereinafter identified as human NK3R) is disclosed which has been prepared by polymerase chain reaction techniques. Also disclosed is the complete sequence of human NK3R complementary DNA; expression systems, including a CHO (chinese hamster ovarian cell line) stable expression system; and an assay using the CHO expression system. Human NK3R can be used in an assay to identify and evaluate entities that bind to the neurokinin-3 receptor.
摘要翻译： 公开了通过聚合酶链反应技术制备的重组人神经激肽-3受体（以下称为人NK3R）。 还公开了人NK3R互补DNA的完整序列; 表达系统，包括CHO（中国仓鼠卵巢细胞系）稳定表达系统; 和使用CHO表达系统的测定。 人NK3R可用于测定以鉴定和评估结合神经激肽-3受体的实体。
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公开(公告)号：US5939263A
公开(公告)日：1999-08-17
申请号：US894236
申请日：1997-07-25
申请人： Margaret A. Cascieri , Douglas J. MacNeil , Lin-Lin Shiao , David H. Weinberg , Carina P. Tan , David L. Linemeyer , Catherine D. Strader
发明人： Margaret A. Cascieri , Douglas J. MacNeil , Lin-Lin Shiao , David H. Weinberg , Carina P. Tan , David L. Linemeyer , Catherine D. Strader
IPC分类号： G01N33/53 , A61K38/00 , C07K14/705 , C07K16/28 , C12N5/10 , C12N15/09 , C12P21/02 , C12R1/91 , G01N33/15 , G01N33/74 , C12Q1/68 , C12N15/85 , C12Q1/02
CPC分类号： C07K14/70571 , G01N33/74 , A61K38/00 , C12N2799/026
摘要： A novel mammalian neuropeptide Y receptor and method of making the receptor are provided. The invention includes DNA encoding the receptor, the receptor, assays employing the receptor, cells expressing the receptor, antibodies which bind specifically to the receptor, RNA encoded by the DNA sequence or its complementary sequence, and single-stranded DNA with a sequence complementary to the RNA which encodes the receptor. The receptor and assays employing the receptor are useful for identifying compounds which bind to the receptor, including specific modulators of the receptor. Such compounds are useful for treating a variety of disease conditions, including obesity, diabetes, anxiety, hypertension, cocaine withdrawal, congestive heart failure, memory enhancement, cardiac and cerebral vasospasm, pheochromocytoma and ganglioneuroblastoma, and Huntington's, Alzheimer's and Parkinson's diseases.
摘要翻译： PCT No.PCT / US96 / 01444 Sec。 371日期1997年7月25日 102（e）日期1997年7月25日PCT 1996年1月30日PCT PCT。 第WO96 / 23809号公报 日期：1996年8月8日提供了一种新型哺乳动物神经肽Y受体及其制备方法。 本发明包括编码受体的DNA，受体，使用受体的测定，表达受体的细胞，与受体特异性结合的抗体，由DNA序列或其互补序列编码的RNA，以及与DNA序列互补的单链DNA 编码受体的RNA。 使用受体的受体和测定法可用于鉴定结合受体的化合物，包括受体的特异性调节剂。 这些化合物可用于治疗各种疾病状况，包括肥胖症，糖尿病，焦虑症，高血压，可卡因戒断，充血性心力衰竭，记忆增强，心脏和脑血管痉挛，嗜铬细胞瘤和神经节细胞瘤，亨廷顿舞蹈症，阿尔茨海默病和帕金森病。
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公开(公告)号：US5621079A
公开(公告)日：1997-04-15
申请号：US415818
申请日：1995-04-03
申请人： Margaret A. Cascieri , David L. Linemeyer , Douglas J. Macneil , Lin-Lin Shiao , Catherine D. Strader , David H. Weinberg , Carina P. Tan
发明人： Margaret A. Cascieri , David L. Linemeyer , Douglas J. Macneil , Lin-Lin Shiao , Catherine D. Strader , David H. Weinberg , Carina P. Tan
IPC分类号： G01N33/53 , A61K38/00 , C07K14/705 , C07K16/28 , C12N5/10 , C12N15/09 , C12P21/02 , C12R1/91 , G01N33/15 , G01N33/74
CPC分类号： C07K14/70571 , G01N33/74 , A61K38/00 , C12N2799/026
摘要： A novel mammalian neuropeptide Y receptor and method of making the receptor are provided. The invention includes DNA encoding the receptor, the receptor, assays employing the receptor, cells expressing the receptor, antibodies which bind specifically to the receptor, RNA encoded by the DNA sequence or its complementary sequence, and single-stranded DNA with a sequence complementary to the RNA which encodes the receptor. The receptor and assays employing the receptor are useful for identifying compounds which bind to the receptor, including specific modulators of the receptor. Such compounds are useful for treating a variety of disease conditions, including obesity, diabetes, anxiety, hypertension, cocaine withdrawal, congestive heart failure, memory enhancement, cardiac and cerebral vasospasm, pheochromocytoma and ganglioneuroblastoma, and Huntington's, Alzheimer's and Parkinson's diseases.
摘要翻译： 提供了一种新型哺乳动物神经肽Y受体和制备受体的方法。 本发明包括编码受体的DNA，受体，使用受体的测定，表达受体的细胞，与受体特异性结合的抗体，由DNA序列或其互补序列编码的RNA，以及与DNA序列互补的单链DNA 编码受体的RNA。 使用受体的受体和测定法可用于鉴定结合受体的化合物，包括受体的特异性调节剂。 这些化合物可用于治疗各种疾病状况，包括肥胖症，糖尿病，焦虑症，高血压，可卡因戒断，充血性心力衰竭，记忆增强，心脏和脑血管痉挛，嗜铬细胞瘤和神经节细胞瘤，亨廷顿舞蹈症，阿尔茨海默病和帕金森病。
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公开(公告)号：US06407207B1
公开(公告)日：2002-06-18
申请号：US08148708
申请日：1993-11-08
IPC分类号： C07K14705
CPC分类号： C07K14/72 , A61K38/00 , C07K14/723
摘要： The present invention is a cloned human BK-2 bradykinin receptor cloned from a human lung fibroblast cell line. A cDNA clone, also part of the instant invention, encodes a novel 364 amino acid protein (the BK-2 receptor) that has the characteristics of a seven transmembrane domain G-protein coupled receptor. The invention is used to express a BK-2 bradykinin receptor in a host mammalian cell to screen for pharmaceutical antagonists or agonists which bind to or interact with the BK-2 bradykinin receptor protein.
摘要翻译： 本发明是从人肺成纤维细胞系克隆的克隆人BK-2缓激肽受体。 cDNA克隆也是本发明的一部分，编码具有七个跨膜结构域G蛋白偶联受体特征的新型364氨基酸蛋白（BK-2受体）。 本发明用于在宿主哺乳动物细胞中表达BK-2缓激肽受体，以筛选与BK-2缓激肽受体蛋白结合或相互作用的药物拮抗剂或激动剂。
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