公开(公告)号：US07413902B2

公开(公告)日：2008-08-19

申请号：US10330873

申请日：2002-12-24

申请人： Andrea G. Bodnar ,  Choy-Pik Chiu ,  Joseph D. Gold ,  Margaret Inokuma ,  James T. Murai ,  Michael D. West

发明人： Andrea G. Bodnar ,  Choy-Pik Chiu ,  Joseph D. Gold ,  Margaret Inokuma ,  James T. Murai ,  Michael D. West

IPC分类号： C12N5/02

CPC分类号： C12N5/0606 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2501/385 ,  C12N2502/13 ,  C12N2510/00

摘要： Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from nucleosides and a pyruvate salt.

摘要翻译： 描述了用于培养灵长类衍生的原始干细胞的方法和材料。 在一个实施方案中，提供了用于生长基本上未分化状态的灵长类来源的原始干细胞的细胞培养基，其包括低渗透压，有效支持灵长类来源的原始干细胞的生长的低内毒素碱性培养基。 基本培养基与有效支持灵长类衍生的原始干细胞的生长和选自饲养细胞的底物和源自饲养细胞的细胞外基质组分的营养血清组合。 培养基还包括非必需氨基酸，抗氧化剂和选自核苷和丙酮酸盐的第一生长因子。

公开(公告)号：US20060040387A1

公开(公告)日：2006-02-23

申请号：US11262633

申请日：2005-10-31

申请人： Gregory Fisk ,  Margaret Inokuma

发明人： Gregory Fisk ,  Margaret Inokuma

IPC分类号： C12N5/08

CPC分类号： C12N5/0676 ,  A01N65/00 ,  A01N2300/00 ,  A61K35/12 ,  A61K35/39 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0618 ,  C12N5/067 ,  C12N5/0678 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/62 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2501/41 ,  C12N2502/02 ,  C12N2502/14 ,  C12N2503/00 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00 ,  G01N33/00 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5067 ,  G01N33/507 ,  G01N33/5073 ,  G01N33/56966

摘要： This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells. High quality populations of islet cells can be produced in commercial quantities for use in research, drug screening, or regenerative medicine.

公开(公告)号：US20050164385A1

公开(公告)日：2005-07-28

申请号：US10948956

申请日：2004-09-24

申请人： Joseph Gold ,  Melissa Carpenter ,  Margaret Inokuma ,  Chunhui Xu

发明人： Joseph Gold ,  Melissa Carpenter ,  Margaret Inokuma ,  Chunhui Xu

IPC分类号： C12N5/0735 ,  C12N5/079 ,  C12N5/0793 ,  C12N15/10 ,  C12N5/08

CPC分类号： C12N5/0622 ,  C12N5/0606 ,  C12N5/0619 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2502/13 ,  C12N2502/99 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2510/04

摘要： This disclosure provides a system for obtaining genetically altered primate pluripotent stem (pPS) cells. The role of the feeder cells is replaced by supporting the culture on an extracellular matrix, and culturing the cells in a conditioned medium. The cells can be genetically altered with a viral vector or DNA/lipid complex, and then selected for successful transfection by drug-resistant phenotype in the transfected cells. The system allows for bulk proliferation of genetically altered pPS cells as important products for use in human therapy or drug screening.

摘要翻译： 本公开提供了用于获得遗传改变的灵长动物多能干细胞（pPS）细胞的系统。 通过将培养物支持在细胞外基质上并在条件培养基中培养细胞来代替饲养细胞的作用。 细胞可以用病毒载体或DNA /脂质复合物进行遗传改变，然后通过转染细胞中耐药性表型选择成功转染。 该系统允许遗传改变的pPS细胞的大量增殖作为用于人类治疗或药物筛选的重要产物。

公开(公告)号：US20050042749A1

公开(公告)日：2005-02-24

申请号：US10873414

申请日：2004-06-21

申请人： Melissa Carpenter ,  Jerrod Denham ,  Margaret Inokuma ,  R. Scott Thies

发明人： Melissa Carpenter ,  Jerrod Denham ,  Margaret Inokuma ,  R. Scott Thies

IPC分类号： A61K35/12 ,  C12N5/0735 ,  C12N5/079 ,  C12N5/0793 ,  C12N5/0797 ,  C12N15/10 ,  C12Q1/68 ,  C12N5/08

CPC分类号： C12N5/0606 ,  A61K35/12 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0623 ,  C12N15/1034 ,  C12N15/1096 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/135 ,  C12N2501/155 ,  C12N2502/13 ,  C12N2502/99 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2510/04 ,  C12Q1/6881 ,  Y02A50/471

摘要： This disclosure provides improved methods for obtaining populations of neural progenitor cells and differentiated neurons from pluripotent stem cells. The technology can be used to produce progenitors that proliferate through at least 40 doublings, while maintaining the ability to differentiate into a variety of different neural phenotypes. Cell populations have been obtained that contain a high proportion of cells staining for tyrosine hydroxylase, which is a feature of dopaminergic neurons. The neural progenitors and terminally differentiated neurons of this invention can be generated in large quantities for use in drug screening and the treatment of clinically important neurological disorders, such as Parkinson's disease.

摘要翻译： 本公开提供了用于从多能干细胞获得神经祖细胞和分化神经元的群体的改进方法。 该技术可用于产生通过至少40次倍增增殖的祖细胞，同时保持分化为多种不同神经表型的能力。 已经获得了含有高比例染色酪氨酸羟化酶的细胞的细胞群，其是多巴胺能神经元的特征。 本发明的神经祖细胞和终末分化的神经元可以大量产生，用于药物筛选和治疗临床上重要的神经障碍，例如帕金森病。

公开(公告)号：US06800480B1

公开(公告)日：2004-10-05

申请号：US09530346

申请日：2000-08-29

申请人： Andrea G. Bodnar ,  Choy-Pik Chiu ,  Joseph D. Gold ,  Margaret Inokuma ,  James T. Murai ,  Michael D. West

发明人： Andrea G. Bodnar ,  Choy-Pik Chiu ,  Joseph D. Gold ,  Margaret Inokuma ,  James T. Murai ,  Michael D. West

IPC分类号： C12N500

CPC分类号： C12N5/0606 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2501/385 ,  C12N2502/13 ,  C12N2510/00

摘要： Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.

摘要翻译： 描述了用于培养灵长类衍生的原始干细胞的方法和材料。 在一个实施方案中，提供了用于生长基本上未分化状态的灵长类来源的原始干细胞的细胞培养基，其包括低渗透压，有效支持灵长类来源的原始干细胞的生长的低内毒素碱性培养基。 基本培养基与有效支持灵长类衍生的原始干细胞的生长的营养血清和选自饲养细胞和源自饲养细胞的细胞外基质组分的底物组合。 该培养基还包括非必需氨基酸，抗氧化剂和选自核苷和丙酮酸盐的第一生长因子。