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公开(公告)号：US20100041150A1
公开(公告)日：2010-02-18
申请号：US12582600
申请日：2009-10-20
CPC分类号： C12N5/0603 , C12N5/0606 , C12N2500/90 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2501/385 , C12N2501/415 , C12N2502/13 , C12N2506/02
摘要： Disclosed herein are cell cultures comprising expanded definitive endoderm cells as well as methods for expanding definitive endoderm cells in culture.
摘要翻译： 本文公开了包含扩增的确定性内胚层细胞的细胞培养物以及用于在培养物中扩增定形内胚层细胞的方法。
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公开(公告)号：US20080107633A1
公开(公告)日：2008-05-08
申请号：US11903408
申请日：2007-09-21
申请人： Melissa Carpenter
发明人： Melissa Carpenter
CPC分类号： A61K35/30 , A61K35/12 , A61K48/00 , A61K2035/126 , C12N5/0623 , C12N2500/25 , C12N2500/34 , C12N2500/90 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/235 , C12N2501/392 , C12N2503/02 , G01N33/5005
摘要： The invention provides a cell culture including proliferating human neural stem cells with a doubling rate faster than thirty days. The invention also provides a cell culture media for proliferating mammalian neural cells including a standard defined culture medium, a carbohydrate source, a buffer, a source of hormones, one or more growth factors that stimulate the proliferation of neural stem cells, and LIF. The invention also provides a method for protecting, repairing or replacing damaged tissue comprising transplanting mammalian neural stem cells formed into neurospheres. The invention also provides a cell culture of differentiated human neural stem cells where the cells are glioblasts. The invention also provides a method of differentiating human neural stem cells in culture media.
摘要翻译： 本发明提供包括增殖人神经干细胞的细胞培养物，其倍增速度比30天更快。 本发明还提供了用于增殖哺乳动物神经细胞的细胞培养基，包括标准定义的培养基，碳水化合物源，缓冲液，激素来源，刺激神经干细胞增殖的一种或多种生长因子和LIF。 本发明还提供了一种保护，修复或替换受损组织的方法，包括移植形成神经球的哺乳动物神经干细胞。 本发明还提供了分化的人神经干细胞的细胞培养物，其中细胞是成胶质细胞。 本发明还提供了一种在培养基中分化人神经干细胞的方法。
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公开(公告)号：US20080020458A9
公开(公告)日：2008-01-24
申请号：US10235094
申请日：2002-09-04
申请人： Ramkumar Mandalam , Chunhui Xu , Joseph Gold , Melissa Carpenter
发明人： Ramkumar Mandalam , Chunhui Xu , Joseph Gold , Melissa Carpenter
IPC分类号： C12N5/08
CPC分类号： C12N15/1096 , C12N5/0062 , C12N5/0068 , C12N5/0075 , C12N5/0603 , C12N5/0606 , C12N5/0607 , C12N5/0619 , C12N5/0622 , C12N5/0662 , C12N5/0671 , C12N5/0672 , C12N5/0678 , C12N5/068 , C12N5/0692 , C12N5/0693 , C12N5/0696 , C12N11/04 , C12N15/1034 , C12N2500/25 , C12N2500/90 , C12N2500/98 , C12N2500/99 , C12N2501/065 , C12N2501/105 , C12N2501/115 , C12N2501/119 , C12N2501/125 , C12N2501/13 , C12N2501/145 , C12N2501/155 , C12N2501/2306 , C12N2501/235 , C12N2501/237 , C12N2501/26 , C12N2501/998 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2506/02 , C12N2510/00 , C12N2510/04 , C12N2533/50 , C12N2533/90
摘要： This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.
摘要翻译： 本公开提供了一种用于培养人多能干细胞的改进系统。 传统上，多能干细胞在一层饲养细胞（如小鼠胚胎成纤维细胞）上培养以防止它们分化。 在这里描述的系统中，饲养细胞的作用由添加到培养环境中的组分代替，其支持快速增殖而不分化。 有效的特征是细胞的合适的支持结构，以及可以在不被另一种细胞类型预处理的情况下可以新鲜添加到培养物中的有效培养基。 在根据本发明的新鲜培养基中培养人胚胎干细胞导致细胞惊人地快速扩张，同时保持分化成代表所有三个胚胎胚层的细胞的能力。 这种新的培养系统允许pPS细胞的大量增殖用于商业生产用于药物筛选和人类治疗的重要产品。
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公开(公告)号：US20060240553A1
公开(公告)日：2006-10-26
申请号：US11356281
申请日：2006-02-15
申请人： Melissa Carpenter
发明人： Melissa Carpenter
IPC分类号： C12N5/08
CPC分类号： A61K35/30 , A61K35/12 , A61K48/00 , A61K2035/126 , C12N5/0623 , C12N2500/25 , C12N2500/34 , C12N2500/90 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/235 , C12N2501/392 , C12N2503/02 , G01N33/5005
摘要： The invention provides a cell culture including proliferating human neural stem cells with a doubling rate faster than thirty days. The invention also provides a cell culture media for proliferating mammalian neural cells including a standard defined culture medium, a carbohydrate source, a buffer, a source of hormones, one or more growth factors that stimulate the proliferation of neural stem cells, and LIF. The invention also provides a method for protecting, repairing or replacing damaged tissue comprising transplanting mammalian neural stem cells formed into neurospheres. The invention also provides a cell culture of differentiated human neural stem cells where the cells are glioblasts. The invention also provides a method of differentiating human neural stem cells in culture media.
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公开(公告)号：US20060078545A1
公开(公告)日：2006-04-13
申请号：US11281040
申请日：2005-11-16
申请人： Melissa Carpenter
发明人： Melissa Carpenter
CPC分类号： C12N5/0618 , A61K35/12 , C12N5/0623 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/13 , C12N2501/135 , C12N2503/02 , C12N2506/02 , C12N2510/00 , C12Q1/6881 , C12Q2600/158
摘要： This invention provides populations of neural progenitor cells, differentiated neurons, glial cells, and astrocytes. The populations are obtained by culturing stem cell populations (such as embryonic stem cells) in a cocktail of growth conditions that initiates differentiation, and establishes the neural progenitor population. The progenitors can be further differentiated in culture into a variety of different neural phenotypes, including dopaminergic neurons. The differentiated cell populations or the neural progenitors can be generated in large quantities for use in drug screening and the treatment of neurological disorders.
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公开(公告)号：US20050164385A1
公开(公告)日：2005-07-28
申请号：US10948956
申请日：2004-09-24
申请人： Joseph Gold , Melissa Carpenter , Margaret Inokuma , Chunhui Xu
发明人： Joseph Gold , Melissa Carpenter , Margaret Inokuma , Chunhui Xu
IPC分类号： C12N5/0735 , C12N5/079 , C12N5/0793 , C12N15/10 , C12N5/08
CPC分类号： C12N5/0622 , C12N5/0606 , C12N5/0619 , C12N2501/13 , C12N2501/155 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2506/02 , C12N2510/00 , C12N2510/04
摘要： This disclosure provides a system for obtaining genetically altered primate pluripotent stem (pPS) cells. The role of the feeder cells is replaced by supporting the culture on an extracellular matrix, and culturing the cells in a conditioned medium. The cells can be genetically altered with a viral vector or DNA/lipid complex, and then selected for successful transfection by drug-resistant phenotype in the transfected cells. The system allows for bulk proliferation of genetically altered pPS cells as important products for use in human therapy or drug screening.
摘要翻译： 本公开提供了用于获得遗传改变的灵长动物多能干细胞（pPS）细胞的系统。 通过将培养物支持在细胞外基质上并在条件培养基中培养细胞来代替饲养细胞的作用。 细胞可以用病毒载体或DNA /脂质复合物进行遗传改变，然后通过转染细胞中耐药性表型选择成功转染。 该系统允许遗传改变的pPS细胞的大量增殖作为用于人类治疗或药物筛选的重要产物。
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7.发明申请Use of TGF beta superfamily antagonists to make dopaminergic neurons from embryonic stem cells 有权使用TGFβ超家族拮抗剂从胚胎干细胞制备多巴胺能神经元公开(公告)号：US20050158855A1
公开(公告)日：2005-07-21
申请号：US11010230
申请日：2004-12-10
申请人： Melissa Carpenter , R. Thies
发明人： Melissa Carpenter , R. Thies
IPC分类号： C12N15/09 , A61K35/12 , C12N5/02 , C12N5/07 , C12N5/073 , C12N5/0735 , C12N5/077 , C12N5/079 , C12N5/0793 , C12N5/10 , C12N15/10 , C12P21/02 , C12Q1/02 , C12R1/91 , C12N5/08
CPC分类号： C12N5/0606 , A61K35/12 , C12N15/1034 , C12N15/1072 , C12N15/1096 , C12N2500/14 , C12N2500/30 , C12N2500/38 , C12N2500/44 , C12N2500/62 , C12N2501/105 , C12N2501/13 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/999 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2510/00 , C12N2510/04 , C12N2533/90
摘要： This invention provides a system for efficiently producing differentiated cells from pluripotent cells, such as human embryonic stem cells. Rather than permitting the cells to form embryoid bodies according to established techniques, differentiation is effected directly in monolayer culture on a suitable solid surface. The cells are either plated directly onto a differentiation-promoting surface, or grown initially on the solid surface in the absence of feeder cells and then exchanged into a medium that assists in the differentiation process. The solid surface and the culture medium can be chosen to direct differentiation down a particular pathway, generating a cell population that is remarkably uniform. The methodology is well adapted to bulk production of committed precursor and terminally differentiated cells for use in drug screening or regenerative medicine.
摘要翻译： 本发明提供了一种用于从多能细胞例如人胚胎干细胞有效产生分化细胞的系统。 不是根据确定的技术使细胞形成胚状体，而是在合适的固体表面的单层培养物中直接进行分化。 将细胞直接铺板到分化促进表面上，或者在不存在饲养细胞的情况下最初在固体表面上生长，然后交换到有助于分化过程的培养基中。 可以选择固体表面和培养基以指导特定途径的分化，产生非常均匀的细胞群体。 该方法适用于大量生产用于药物筛选或再生医学的定型前体和终末分化细胞。
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8.发明申请Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease 审中-公开多巴胺能神经元和用于治疗帕金森病的增殖能力前体细胞公开(公告)号：US20050042749A1
公开(公告)日：2005-02-24
申请号：US10873414
申请日：2004-06-21
IPC分类号： A61K35/12 , C12N5/0735 , C12N5/079 , C12N5/0793 , C12N5/0797 , C12N15/10 , C12Q1/68 , C12N5/08
CPC分类号： C12N5/0606 , A61K35/12 , C12N5/0618 , C12N5/0619 , C12N5/0622 , C12N5/0623 , C12N15/1034 , C12N15/1096 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/13 , C12N2501/135 , C12N2501/155 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2506/02 , C12N2510/00 , C12N2510/04 , C12Q1/6881 , Y02A50/471
摘要： This disclosure provides improved methods for obtaining populations of neural progenitor cells and differentiated neurons from pluripotent stem cells. The technology can be used to produce progenitors that proliferate through at least 40 doublings, while maintaining the ability to differentiate into a variety of different neural phenotypes. Cell populations have been obtained that contain a high proportion of cells staining for tyrosine hydroxylase, which is a feature of dopaminergic neurons. The neural progenitors and terminally differentiated neurons of this invention can be generated in large quantities for use in drug screening and the treatment of clinically important neurological disorders, such as Parkinson's disease.
摘要翻译： 本公开提供了用于从多能干细胞获得神经祖细胞和分化神经元的群体的改进方法。 该技术可用于产生通过至少40次倍增增殖的祖细胞，同时保持分化为多种不同神经表型的能力。 已经获得了含有高比例染色酪氨酸羟化酶的细胞的细胞群，其是多巴胺能神经元的特征。 本发明的神经祖细胞和终末分化的神经元可以大量产生，用于药物筛选和治疗临床上重要的神经障碍，例如帕金森病。
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公开(公告)号：US20050037493A1
公开(公告)日：2005-02-17
申请号：US10810311
申请日：2004-03-26
申请人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
发明人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2500/62 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/13 , C12N2501/148 , C12N2501/155 , C12N2501/23 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2502/13 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。
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公开(公告)号：US20050153445A1
公开(公告)日：2005-07-14
申请号：US11010140
申请日：2004-12-10
申请人： Ramkumar Mandalam , Chunhui Xu , Joseph Gold , Melissa Carpenter
发明人： Ramkumar Mandalam , Chunhui Xu , Joseph Gold , Melissa Carpenter
IPC分类号： C12N15/09 , A61K35/12 , A61P43/00 , C12N5/0735 , C12N5/077 , C12N5/079 , C12N5/0793 , C12N5/10 , C12N15/10 , C12N5/08
CPC分类号： C12N15/1096 , C12N5/0062 , C12N5/0068 , C12N5/0075 , C12N5/0603 , C12N5/0606 , C12N5/0607 , C12N5/0619 , C12N5/0622 , C12N5/0662 , C12N5/0671 , C12N5/0672 , C12N5/0678 , C12N5/068 , C12N5/0692 , C12N5/0693 , C12N5/0696 , C12N11/04 , C12N15/1034 , C12N2500/25 , C12N2500/90 , C12N2500/98 , C12N2500/99 , C12N2501/065 , C12N2501/105 , C12N2501/115 , C12N2501/119 , C12N2501/125 , C12N2501/13 , C12N2501/145 , C12N2501/155 , C12N2501/2306 , C12N2501/235 , C12N2501/237 , C12N2501/26 , C12N2501/998 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2506/02 , C12N2510/00 , C12N2510/04 , C12N2533/50 , C12N2533/90
摘要： This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.
摘要翻译： 本公开提供了一种用于培养人多能干细胞的改进系统。 传统上，多能干细胞在一层饲养细胞（如小鼠胚胎成纤维细胞）上培养以防止它们分化。 在这里描述的系统中，饲养细胞的作用由添加到培养环境中的组分代替，其支持快速增殖而不分化。 有效的特征是细胞的合适的支持结构，以及可以在不被另一种细胞类型预处理的情况下可以新鲜添加到培养物中的有效培养基。 在根据本发明的新鲜培养基中培养人胚胎干细胞导致细胞惊人地快速扩张，同时保持分化成代表所有三个胚胎胚层的细胞的能力。 这种新的培养系统允许pPS细胞的大量增殖用于商业生产用于药物筛选和人类治疗的重要产品。
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