公开(公告)号：US20060246499A1

公开(公告)日：2006-11-02

申请号：US11444819

申请日：2006-05-19

Applicant: Mark Spector ,  David Stenger ,  Charles Patterson ,  Brett Martin ,  Paul Charles

Inventor： Mark Spector ,  David Stenger ,  Charles Patterson ,  Brett Martin ,  Paul Charles

IPC: C12Q1/68 ,  C08G63/91 ,  C12M1/34

CPC classification number: G01N33/548 ,  G01N33/54353

Abstract: We describe the novel use of a sugar-containing hydrogels as very highly porous, aqueous support material for the immobilization of oligonucleotides, peptides, proteins, antigens, antibodies, polysaccharides, and other biomolecules for sensor applications. The unusually large sizes of the interconnected pores allow large target molecules to pass rapidly into and through the gel and bind to immobilized biomolecules. An additional advantage of the sugar-containing hydrogels are their extremely low non-specific absorption of labeled target molecules, providing low background levels. State-of-the-art hydrogel materials do not have this type of homogeneous interconnected macroporosity, thus large target molecules cannot readily diffuse through them. In addition, they nearly always experience non-specific (background) absorption of labeled target molecules, limiting their usefulness in sensor applications. This invention provides a method for preparing a sugar polyacrylate hydrogel with functional chemical groups which covalently bond oligonucleotides and peptides. A method for copolymerizing acrylate-terminated oligonucleotides with sugar acrylate monomers and diacrylate cross-linking agents is also provided.

Abstract translation: 我们描述了含糖水凝胶作为用于固定寡核苷酸，肽，蛋白质，抗原，抗体，多糖和其他用于传感器应用的生物分子的非常高度多孔的水性载体材料的新用途。 互连孔的异常大尺寸允许大的目标分子快速通过并穿过凝胶并结合固定的生物分子。 含糖水凝胶的另外的优点是它们非常低的标记靶分子的非特异性吸收，提供低的背景水平。 最先进的水凝胶材料不具有这种类型的均匀相互连接的大孔隙度，因此大的靶分子不能容易地通过它们扩散。 此外，它们几乎总是经历非特异性（背景）吸收标记的靶分子，限制了它们在传感器应用中的用途。 本发明提供了制备具有共价键合寡核苷酸和肽的功能化学基团的糖聚丙烯酸酯水凝胶的方法。 还提供了将丙烯酸酯封端的寡核苷酸与糖丙酯单体和二丙烯酸酯交联剂共聚的方法。

公开(公告)号：US20050019884A1

公开(公告)日：2005-01-27

申请号：US10627143

申请日：2003-07-25

Applicant: Mark Spector ,  David Stenger ,  Charles Patterson ,  Brett Martin ,  Paul Charles

Inventor： Mark Spector ,  David Stenger ,  Charles Patterson ,  Brett Martin ,  Paul Charles

IPC: A61K9/00 ,  C07H1/00 ,  C07H15/00 ,  C08J3/00 ,  C12M1/34 ,  C12N11/00 ,  C12Q20060101 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/548

CPC classification number: G01N33/548 ,  G01N33/54353

Abstract: We describe the novel use of a sugar-containing hydrogels as very highly porous, aqueous support material for the immobilization of oligonucleotides, peptides, proteins, antigens, antibodies, polysaccharides, and other biomolecules for sensor applications. The unusually large sizes of the interconnected pores allow large target molecules to pass rapidly into and through the gel and bind to immobilized biomolecules. An additional advantage of the sugar-containing hydrogels are their extremely low non-specific absorption of labeled target molecules, providing low background levels. State-of-the-art hydrogel materials do not have this type of homogeneous interconnected macroporosity, thus large target molecules cannot readily diffuse through them. In addition, they nearly always experience non-specific (background) absorption of labeled target molecules, limiting their usefulness in sensor applications. This invention provides a method for preparing a sugar polyacrylate hydrogel with functional chemical groups which covalently bond oligonucleotides and peptides. A method for copolymerizing acrylate-terminated oligonucleotides with sugar acrylate monomers and diacrylate cross-linking agents is also provided.

Abstract translation: 我们描述了含糖水凝胶作为用于固定寡核苷酸，肽，蛋白质，抗原，抗体，多糖和其他用于传感器应用的生物分子的非常高度多孔的水性载体材料的新用途。 互连孔的异常大尺寸允许大的目标分子快速通过并穿过凝胶并结合固定的生物分子。 含糖水凝胶的另外的优点是它们非常低的标记靶分子的非特异性吸收，提供低的背景水平。 最先进的水凝胶材料不具有这种类型的均匀相互连接的大孔隙度，因此大的靶分子不能容易地通过它们扩散。 此外，它们几乎总是经历非特异性（背景）吸收标记的靶分子，限制了它们在传感器应用中的用途。 本发明提供了制备具有共价键合寡核苷酸和肽的功能化学基团的糖聚丙烯酸酯水凝胶的方法。 还提供了将丙烯酸酯封端的寡核苷酸与糖丙酯单体和二丙烯酸酯交联剂共聚的方法。

公开(公告)号：US20080097918A1

公开(公告)日：2008-04-24

申请号：US11975796

申请日：2007-10-22

Applicant: Mark Spector ,  Michael Neuss ,  Richard Levy ,  Stanley Forston

Inventor： Mark Spector ,  Michael Neuss ,  Richard Levy ,  Stanley Forston

IPC: G06Q10/00 ,  G06Q50/00 ,  H04L9/00

CPC classification number: G06Q50/24 ,  G06F19/3418 ,  G06F19/3456 ,  G06F19/3481 ,  G06Q50/22 ,  G16H10/60 ,  G16H40/20 ,  G16H50/20

Abstract: An Internet-based, or Web-based, customizable clinical (patients' records and care) information system (“CIS”) is provided. More specifically, the clinical information system is Web/Internet based, whether it utilizes a browser-type user interface or a distributed application-type user interface; the clinical information system may include automatic disease staging and associated treatment planning and/or scheduling; the clinical information system may track certain events/submissions and sort such events/submissions into a physician's in-box for on-line approval by the physician, where such approval causes the event/submission to become an addendum to the patient's record; the clinical information system may be customizable by an administrator; the clinical information system may establish, and make available for on-line review and approval, patient care or standing orders over a weekend; the clinical information system may utilize patients' photographs to ensure accurate identification and proper treatment; and the clinical information system may create and store an audit trail record for all significant events.

Abstract translation: 提供基于互联网或基于网络的可定制临床（患者记录和护理）信息系统（“CIS”）。 更具体地说，临床信息系统是基于Web / Internet的，无论是利用浏览器型用户接口还是分布式应用型用户界面; 临床信息系统可以包括自动疾病分期和相关治疗计划和/或调度; 临床信息系统可以跟踪某些事件/提交，并将这样的事件/提交分类到医生的收件箱中，以由医生在线批准，其中这种批准导致事件/提交成为患者记录的附录; 临床信息系统可以由管理员定制; 临床信息系统可以在一个周末建立并提供在线审查和批准病人护理或常规订单; 临床信息系统可以利用患者的照片来确保准确的识别和适当的治疗; 临床信息系统可以创建和存储所有重要事件的审计跟踪记录。

公开(公告)号：US20060062840A1

公开(公告)日：2006-03-23

申请号：US11215199

申请日：2005-08-29

Applicant: Ronald Price ,  Joel Schnur ,  Banahalli Ratna ,  Mark Spector

Inventor： Ronald Price ,  Joel Schnur ,  Banahalli Ratna ,  Mark Spector

IPC: A61K9/127 ,  B29B15/00

CPC classification number: C11B3/00

Abstract: The wall thickness of lipid microtubules are controlled by selecting a methanol/water system and determining the required amount of a lipid to form the desired wall thickness. The lipid is dissolved in a small portion of the heated methanol and that clear solution is added to the remaining amount of the heated methanol/water system. By slowly cooling the solution, microtubules are formed which have the desired wall thickness. Preferred microtubules have a wall thickness of just 2 bilayers and they are robust so they can be further coated. They can be made with a large aspect ratio and with lengths of greater than 250 microns. The process permits production of microtubules in very high yields.

Abstract translation: 通过选择甲醇/水系统并确定所需量的脂质以形成所需的壁厚来控制脂质微管的壁厚。 将脂质溶解在一小部分加热的甲醇中，并将澄清溶液加入剩余量的加热的甲醇/水体系中。 通过缓慢冷却溶液，形成具有所需壁厚的微管。 优选的微管的壁厚仅为2双层，并且它们是坚固的，因此它们可以被进一步涂覆。 它们可以制成具有大的纵横比并且长度大于250微米。 该方法允许以非常高的产率生产微管。

公开(公告)号：US06013206A

公开(公告)日：2000-01-11

申请号：US80194

申请日：1998-05-18

Applicant: Ronald R. Price ,  Joel M. Schnur ,  Paul E. Schoen ,  Dan Zabetakis ,  Mark Spector

Inventor： Ronald R. Price ,  Joel M. Schnur ,  Paul E. Schoen ,  Dan Zabetakis ,  Mark Spector

IPC: A61K9/127 ,  C23C18/16 ,  B01J13/02 ,  B01J13/04

CPC classification number: A61K9/1274 ,  C23C18/1633 ,  C23C18/1641 ,  C23C18/1644 ,  C23C18/2086 ,  Y10S977/797 ,  Y10T428/2984 ,  Y10T428/2989

Abstract: Lipid microtubules having a controlled bilayer structure and high aspect io are formed in a methanol/ethanol/water solvent system. The lipid microtubules may then be catalyzed (e.g., with a palladium/tin catalyst) in an acidified catalytic bath having no more than about 30 g of catalytic salts. These catalyzed microtubules are then metallized using a diluted plating bath with replenishment of the plating bath as needed to obtain the desired metallization thickness.

Abstract translation: 在甲醇/乙醇/水溶剂体系中形成具有受控双层结构和高纵横比的脂质微管。 然后可以在不超过约30g催化盐的酸化催化浴中催化脂质微管（例如用钯/锡催化剂）。 然后根据需要，使用稀释电镀浴对这些催化的微管进行金属化，补充电镀浴以获得所需的金属化厚度。