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公开(公告)号：US08178322B2
公开(公告)日：2012-05-15
申请号：US12541204
申请日：2009-08-14
申请人： Masayuki Araki , Yusuke Takahashi , Akihiro Watanabe , Fumito Ohnishi , Takahiro Asano , Kazuya Kondo , Wataru Hibino , Shintaro Iwatani , Satoshi Okutani
发明人： Masayuki Araki , Yusuke Takahashi , Akihiro Watanabe , Fumito Ohnishi , Takahiro Asano , Kazuya Kondo , Wataru Hibino , Shintaro Iwatani , Satoshi Okutani
IPC分类号： C12P19/40 , C12P19/30 , C12P19/34 , C12P13/04 , C12P13/22 , C12P13/12 , C12P13/08 , C12P13/06
摘要： A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m3 or lower after either precipitation of the crystals or addition of the seed crystals.
摘要翻译： 描述了通过在含有搅拌叶轮的发酵罐中培养具有在液体培养基中产生L-氨基酸或核酸的能力的微生物来生产L-氨基酸或核酸的方法，并且任选地加入晶种 在培养基中产生并积累L-氨基酸或核酸的晶体并从培养物中收集L-氨基酸或核酸的晶体的培养基中。 搅拌叶轮的功率密度在晶体沉淀或加入晶种后控制在2.4 kW / m3或更低。
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公开(公告)号：US6004773A
公开(公告)日：1999-12-21
申请号：US985908
申请日：1997-12-05
IPC分类号： C12N1/21 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/53 , C12N15/54 , C12N15/60 , C12N15/77 , C12P13/08 , C12P1/00 , C07H21/04 , C12N15/63 , C12P21/02
CPC分类号： C12N9/001 , C12N15/77 , C12N9/1096 , C12N9/1217 , C12N9/88 , C12P13/08
摘要： A coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a dihydrodipicolinate reductase, an enhanced DNA sequence coding for dihydropicolinate reductase, an enhance DNA sequence coding for dihydropicolinate synthase, an enhanced DNA sequence coding for diaminopimelate decarboxylase and an enhanced DNA sequence coding for aspartate aminotransferase; a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture; and a recombinant DNA usable for production of the coryneform bacterium.
摘要翻译： 含有赖氨酸和L-苏氨酸的反馈抑制基本脱敏的天冬氨酸激酶的棒状细菌，包含编码二氢吡啶二羧酸还原酶的增强型DNA序列，编码二氢吡啶啉酮还原酶的增强型DNA序列，编码二氢吡啶酸盐的增强型DNA序列 合成酶，编码二氨基庚二酸脱羧酶的增强的DNA序列和编码天冬氨酸转氨酶的增强的DNA序列; 一种生产L-赖氨酸的方法，包括以下步骤：在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，并从培养物中收集L-赖氨酸; 以及可用于生产棒状细菌的重组DNA。
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公开(公告)号：US20100062493A1
公开(公告)日：2010-03-11
申请号：US12541204
申请日：2009-08-14
申请人： Masayuki Araki , Yusuke Takahashi , Akihiro Watanabe , Fumito Ohnishi , Takahiro Asano , Kazuya Kondo , Wataru Hibino , Shintaro Iwatani , Satoshi Okutani
发明人： Masayuki Araki , Yusuke Takahashi , Akihiro Watanabe , Fumito Ohnishi , Takahiro Asano , Kazuya Kondo , Wataru Hibino , Shintaro Iwatani , Satoshi Okutani
摘要： A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m3 or lower after either precipitation of the crystals or addition of the seed crystals.
摘要翻译： 描述了通过在含有搅拌叶轮的发酵罐中培养具有在液体培养基中产生L-氨基酸或核酸的能力的微生物来生产L-氨基酸或核酸的方法，并且任选地加入晶种 在培养基中产生并积累L-氨基酸或核酸的晶体并从培养物中收集L-氨基酸或核酸的晶体的培养基中。 搅拌叶轮的功率密度在晶体沉淀或加入晶种后控制在2.4 kW / m3或更低。
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