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    Method for producing L-lysine
    1.
    发明授权
    Method for producing L-lysine 失效
    生产L-赖氨酸的方法

    公开(公告)号：US6004773A

    公开(公告)日：1999-12-21

    申请号：US985908

    申请日：1997-12-05

    申请人： Masayuki Araki ,  Masakazu Sugimoto ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    发明人： Masayuki Araki ,  Masakazu Sugimoto ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N15/53 ,  C12N15/54 ,  C12N15/60 ,  C12N15/77 ,  C12P13/08 ,  C12P1/00 ,  C07H21/04 ,  C12N15/63 ,  C12P21/02

    CPC分类号： C12N9/001 ,  C12N15/77 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/88 ,  C12P13/08

    摘要： A coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a dihydrodipicolinate reductase, an enhanced DNA sequence coding for dihydropicolinate reductase, an enhance DNA sequence coding for dihydropicolinate synthase, an enhanced DNA sequence coding for diaminopimelate decarboxylase and an enhanced DNA sequence coding for aspartate aminotransferase; a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture; and a recombinant DNA usable for production of the coryneform bacterium.

    摘要翻译： 含有赖氨酸和L-苏氨酸的反馈抑制基本脱敏的天冬氨酸激酶的棒状细菌，包含编码二氢吡啶二羧酸还原酶的增强型DNA序列，编码二氢吡啶啉酮还原酶的增强型DNA序列，编码二氢吡啶酸盐的增强型DNA序列 合成酶，编码二氨基庚二酸脱羧酶的增强的DNA序列和编码天冬氨酸转氨酶的增强的DNA序列; 一种生产L-赖氨酸的方法，包括以下步骤：在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，并从培养物中收集L-赖氨酸; 以及可用于生产棒状细菌的重组DNA。

    Method for producing L-lysine
    4.
    发明授权
    Method for producing L-lysine 失效
    生产L-赖氨酸的方法

    公开(公告)号：US06221636B1

    公开(公告)日：2001-04-24

    申请号：US08985916

    申请日：1997-12-05

    申请人： Atsushi Hayakawa ,  Masakazu Sugimoto ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    发明人： Atsushi Hayakawa ,  Masakazu Sugimoto ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    IPC分类号： C12P1308

    CPC分类号： C12N9/1217 ,  C12N9/88 ,  C12P13/08

    摘要： A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA sequence coding for a diaminopimelate decarboxylase; a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a diaminopimelate decarboxylase; and a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture.

    摘要翻译： 在棒状杆菌细胞的细胞中自主复制的重组DNA，其中包含编码天冬氨酸激酶的DNA序列，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，以及编码二氨基庚二酸脱羧酶的DNA序列; 携带天冬氨酸激酶的棒状细菌，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，并且包含编码二氨基庚二酸脱羧酶的增强的DNA序列; 以及L-赖氨酸的制造方法，其特征在于，在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，从培养物中收集L-赖氨酸。

    Methods of producing L-lysine and L-glutamic acid by fermentation
    7.
    发明授权
    Methods of producing L-lysine and L-glutamic acid by fermentation 失效
    通过发酵生产L-赖氨酸和L-谷氨酸的方法

    公开(公告)号：US5846790A

    公开(公告)日：1998-12-08

    申请号：US776597

    申请日：1997-02-18

    申请人： Eiichiro Kimura ,  Yoko Asakura ,  Akinori Uehara ,  Sumio Inoue ,  Yoshio Kawahara ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    发明人： Eiichiro Kimura ,  Yoko Asakura ,  Akinori Uehara ,  Sumio Inoue ,  Yoshio Kawahara ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    IPC分类号： C12N1/21 ,  C12N15/09 ,  C12P13/08 ,  C12P13/14

    CPC分类号： C12P13/14 ,  C12P13/08 ,  Y10S435/84 ,  Y10S435/843

    摘要： A mutant strain having an ability to produce L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to produce and accumulate L-glutamic acid in the medium. A mutant strain having an ability to produce L-lysine and L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent and giving L-lysine productivity to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to simultaneously produce and accumulate L-lysine and L-glutamic acid in the medium.

    摘要翻译： PCT No.PCT / JP95 / 01586 Sec。 371日期1997年2月18日 102（e）1997年2月18日PCT PCT 1995年8月9日PCT公布。 出版物WO96 / 06180 日期：1996年2月29日在具有生物素活性抑制剂的情况下，在含有过量生物素的培养基中，不存在生成L-谷氨酸的能力的突变株是通过赋予生物素作用抑制 代谢产L-谷氨酸棒状细菌。 将该菌株在液体培养基中培养以在培养基中产生并积累L-谷氨酸。 通过赋予生物素活性抑制剂的温度敏感度，获得在含有过量生物素的培养基中不存在任何生物素活性抑制剂时能够产生L-赖氨酸和L-谷氨酸的突变株 并向L-谷氨酸棒状杆菌产生细菌提供L-赖氨酸生产力。 该菌株在液体培养基中培养，以在培养基中同时产生和积累L-赖氨酸和L-谷氨酸。