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公开(公告)号：US08722326B2
公开(公告)日：2014-05-13
申请号：US11981661
申请日：2007-10-31
CPC分类号： C12Q1/6837 , C12Q1/6874 , C12Q2531/125 , C12Q2525/151 , C12Q2521/313
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.
摘要翻译： 本发明涉及使用分散在靶多核苷酸中的衔接子获得靶序列的核苷酸序列信息的方法和组合物。 序列信息可以是新的，例如 测序未知核酸，重新测序或基因分型。 本发明优选地包括在多核苷酸的靶多核苷酸或片段内的间隔位置插入多个衔接子的方法。 这样的衔接子可以用作使用各种测序化学品询问相邻序列的平台，例如通过引物延伸，探针连接等鉴定核苷酸的测序化学物质。 在本发明中包括用于将已知衔接子序列插入靶序列的方法和组合物，使得与适配器存在连续靶序列的中断。 通过对适配器的“上游”和“下游”进行排序，可以完成整个靶序列的识别。
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公开(公告)号：US20090155781A1
公开(公告)日：2009-06-18
申请号：US11981761
申请日：2007-10-31
CPC分类号： C12Q1/6874 , C12N15/64 , C12N15/66 , Y10T436/143333 , C12Q2521/313 , C12Q2525/191 , C12Q2525/151 , C12Q2525/131 , C12Q2565/518 , C12Q2533/107 , C12Q2531/125
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.
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公开(公告)号：US20090118488A1
公开(公告)日：2009-05-07
申请号：US11981793
申请日：2007-10-31
IPC分类号： C07H21/04
CPC分类号： C12Q1/6874 , C12N15/64 , C12N15/66 , Y10T436/143333 , C12Q2521/313 , C12Q2525/191 , C12Q2525/151 , C12Q2525/131 , C12Q2565/518 , C12Q2533/107 , C12Q2531/125
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.
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公开(公告)号：US09228228B2
公开(公告)日：2016-01-05
申请号：US13035740
申请日：2011-02-25
申请人： Radoje T. Drmanac , Matthew Callow
发明人： Radoje T. Drmanac , Matthew Callow
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6874 , C12Q2565/501 , C12Q2539/103 , C12Q2531/119
摘要： The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.
摘要翻译： 本发明一般涉及多核苷酸的分析，特别是衍生自基因组DNA的多核苷酸。 本发明提供了用于这种分析的方法，组合物和系统。 本发明所涵盖的是多核苷酸阵列，其中多核苷酸经历了多轮扩增，以增加与单个多核苷酸分子相关的信号强度。
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公开(公告)号：US07910354B2
公开(公告)日：2011-03-22
申请号：US11927356
申请日：2007-10-29
申请人： Radoje T. Drmanac , Matthew Callow
发明人： Radoje T. Drmanac , Matthew Callow
IPC分类号： C12M1/34
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6874 , C12Q2565/501 , C12Q2539/103 , C12Q2531/119
摘要： The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.
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公开(公告)号：US20090005252A1
公开(公告)日：2009-01-01
申请号：US11981661
申请日：2007-10-31
CPC分类号： C12Q1/6837 , C12Q1/6874 , C12Q2531/125 , C12Q2525/151 , C12Q2521/313
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.
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公开(公告)号：US20120004126A1
公开(公告)日：2012-01-05
申请号：US13035740
申请日：2011-02-25
申请人： Radoje T. Drmanac , Matthew Callow
发明人： Radoje T. Drmanac , Matthew Callow
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6874 , C12Q2565/501 , C12Q2539/103 , C12Q2531/119
摘要： The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.
摘要翻译： 本发明一般涉及多核苷酸的分析，特别是衍生自基因组DNA的多核苷酸。 本发明提供了用于这种分析的方法，组合物和系统。 本发明所涵盖的是多核苷酸阵列，其中多核苷酸经历了多轮扩增，以增加与单个多核苷酸分子相关的信号强度。
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公开(公告)号：US07910302B2
公开(公告)日：2011-03-22
申请号：US11927388
申请日：2007-10-29
申请人： Radoje T Drmanac , Matthew Callow
发明人： Radoje T Drmanac , Matthew Callow
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6874 , C12Q2565/501 , C12Q2539/103 , C12Q2531/119
摘要： The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.
摘要翻译： 本发明一般涉及多核苷酸的分析，特别是衍生自基因组DNA的多核苷酸。 本发明提供了用于这种分析的方法，组合物和系统。 本发明所涵盖的是多核苷酸阵列，其中多核苷酸经历了多轮扩增，以增加与单个多核苷酸分子相关的信号强度。
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公开(公告)号：US20090143235A1
公开(公告)日：2009-06-04
申请号：US11927388
申请日：2007-10-29
申请人： Radoje Drmanac , Matthew Callow
发明人： Radoje Drmanac , Matthew Callow
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6874 , C12Q2565/501 , C12Q2539/103 , C12Q2531/119
摘要： The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.
摘要翻译： 本发明一般涉及多核苷酸的分析，特别是衍生自基因组DNA的多核苷酸。 本发明提供了用于这种分析的方法，组合物和系统。 本发明所涵盖的是多核苷酸阵列，其中多核苷酸经历了多轮扩增，以增加与单个多核苷酸分子相关的信号强度。
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公开(公告)号：US20070099208A1
公开(公告)日：2007-05-03
申请号：US11451691
申请日：2006-06-13
申请人： Radoje Drmanac , Matthew Callow , Snezana Drmanac , Brian Hauser , George Yeung
发明人： Radoje Drmanac , Matthew Callow , Snezana Drmanac , Brian Hauser , George Yeung
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
摘要翻译： 提供单个分子的随机阵列用于进行大规模分析，特别是生物分子，例如基因组DNA，cDNAs，蛋白质等。 在一个方面，本发明的阵列包括随机地设置在离散的间隔开的区域的规则阵列上的DNA片段的连接体，使得基本上所有这些区域都包含不超过单个并联体。 优选地，这样的区域具有基本上小于1mum 2的面积，并且具有最接近的距离，其允许每厘米2的单分子的光学分辨率 。 许多分析化学物质可以应用于本发明的随机阵列，包括杂交化学测序，合成化学测序，SNP检测化学等，以大大扩展这些技术的规模和潜在应用。
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