公开(公告)号：US5922602A

公开(公告)日：1999-07-13

申请号：US260546

申请日：1994-06-16

申请人： Monto Hiroshi Kumagai ,  Guy Richard della-Cioppa ,  Jonathan Donson ,  Damon Alan Harvey ,  Laurence K. Grill

发明人： Monto Hiroshi Kumagai ,  Guy Richard della-Cioppa ,  Jonathan Donson ,  Damon Alan Harvey ,  Laurence K. Grill

IPC分类号： A01H5/00 ,  C07K14/08 ,  C07K14/095 ,  C07K14/415 ,  C07K14/705 ,  C12N1/19 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/24 ,  C12N9/72 ,  C12N9/78 ,  C12N9/84 ,  C12N15/09 ,  C12N15/113 ,  C12N15/12 ,  C12N15/40 ,  C12N15/53 ,  C12N15/82 ,  C12N15/83 ,  C12N15/86 ,  C12P21/04 ,  C12P41/00 ,  C12N15/63

CPC分类号： C12N15/1137 ,  C07K14/005 ,  C07K14/415 ,  C07K14/70571 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8249 ,  C12N15/825 ,  C12N15/8289 ,  C12N15/86 ,  C12N9/00 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1074 ,  C12N9/1085 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/6459 ,  C12N9/78 ,  C12N9/84 ,  C12P41/003 ,  C12Y114/18001 ,  C12Y302/01031 ,  C12Y304/21069 ,  C12N2710/22043 ,  C12N2710/22062 ,  C12N2750/12043 ,  C12N2770/00022 ,  C12N2770/00043 ,  C12N2770/00051 ,  C12N2770/32643 ,  C12N2770/32662 ,  C12N2770/32722 ,  C12N2770/32743 ,  C12N2770/32762 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2799/021 ,  C12N2810/609 ,  G01N2333/4353

摘要： The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.

公开(公告)号：US5705624A

公开(公告)日：1998-01-06

申请号：US579667

申请日：1995-12-27

申请人： Wayne Paul Fitzmaurice ,  Gary Mark Hellmann ,  Laurence Kay Grill ,  Monto Hiroshi Kumagai ,  Guy Richard della-Cioppa

发明人： Wayne Paul Fitzmaurice ,  Gary Mark Hellmann ,  Laurence Kay Grill ,  Monto Hiroshi Kumagai ,  Guy Richard della-Cioppa

IPC分类号： C12N9/00 ,  C07H21/04

CPC分类号： C12N9/00

摘要： DNA sequences isolated from Nicotiana species (e.g., Nicotiana tabacum and Nicotiana benthamiana) and encoding polypeptides having enzymatic activity for producing phytoene, and the polypeptides encoded, are provided.

摘要翻译： 提供了从烟草属（Nicotiana tabacum）和本烟草属烟草属（Nicotiana benhamiana））分离的DNA序列，并编码具有用于产生番茄红素的酶活性的多肽和编码的多肽。