公开(公告)号：US20140325707A1

公开(公告)日：2014-10-30

申请号：US14225823

申请日：2014-03-26

申请人： Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.

发明人： Ilan SELA ,  Haim David Rabinowitch ,  Leonid Chernin ,  Rita Mozes-Koch

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  C07K14/21 ,  C12N15/52 ,  C12N15/8203 ,  C12N15/86 ,  C12N2750/12022 ,  C12N2750/12043 ,  Y02A40/146

摘要： The present invention provides means and methods for producing plants with enhanced growth and yield. Particularly, the present invention provides plants to which an expression construct comprising the entire prn operon was introduced, wherein said plants produce pyrrolnitrin and show enhanced growth and yield production compared to corresponding control plants not harboring the prn operon.

摘要翻译： 本发明提供用于生产具有增加的生长和产量的植物的方法和方法。 特别地，本发明提供了引入包含整个prn操纵子的表达构建体的植物，其中与不含有prn操纵子的相应对照植物相比，所述植物产生吡咯烷腈并显示增强的生长和产量产生。

公开(公告)号：US20040142477A1

公开(公告)日：2004-07-22

申请号：US10773601

申请日：2004-02-06

申请人： Large Scale Biology Corporation

发明人： Monto H. Kumagai ,  Guy R. Della-Cioppa ,  Jonathan Donson ,  Damon A. Harvey ,  Laurence K. Grill

IPC分类号： A01H001/00 ,  C12N015/82 ,  C12N015/87 ,  C12N005/04

CPC分类号： C12N15/1137 ,  C07K14/005 ,  C07K14/415 ,  C07K14/70571 ,  C12N9/00 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1074 ,  C12N9/1085 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/6459 ,  C12N9/78 ,  C12N9/84 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8249 ,  C12N15/825 ,  C12N15/8289 ,  C12N15/86 ,  C12N2710/22043 ,  C12N2710/22062 ,  C12N2750/12043 ,  C12N2770/00022 ,  C12N2770/00043 ,  C12N2770/00051 ,  C12N2770/32643 ,  C12N2770/32662 ,  C12N2770/32722 ,  C12N2770/32743 ,  C12N2770/32762 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2799/021 ,  C12N2810/609 ,  C12P41/003 ,  C12Y114/18001 ,  C12Y302/01031 ,  C12Y304/21069 ,  G01N2333/4353

摘要： The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.

摘要翻译： 本发明提供了用于在真核细胞的细胞质中表达抑制性RNA的新型遗传构建体。 抑制性RNA可以是反义RNA或共抑制RNA，并且起减少目标细胞中目的基因表达的作用。 本发明的遗传结构能够在真核细胞的细胞质中复制并且包含与编码多核苷酸的功能性组合的启动子区。 遗传结构可以设计成在植物细胞，酵母细胞和哺乳动物细胞的细胞质中复制。 当感兴趣的真核细胞是植物细胞时，遗传构建优选衍生自植物RNA病毒。 植物RNA病毒衍生的遗传结构可以使用植物病毒亚基因组启动子，包括与RNA编码区域功能组合的来自烟草花叶病毒的亚基因组启动子。 在本发明的一个优选实施方案中，诱导植物细胞产生升高水平的类胡萝卜素八氢番茄红素。 通过使用本发明的载体抑制酶八氢番茄红素去饱和酶的表达来实现提高的八氢番茄聚糖水平。

公开(公告)号：US5889190A

公开(公告)日：1999-03-30

申请号：US480432

申请日：1995-06-07

申请人： Jon Donson ,  William O. Dawson ,  George L. Grantham ,  Thomas H. Turpen ,  Ann Myers Turpen ,  Stephen J. Garger ,  Laurence K. Grill

发明人： Jon Donson ,  William O. Dawson ,  George L. Grantham ,  Thomas H. Turpen ,  Ann Myers Turpen ,  Stephen J. Garger ,  Laurence K. Grill

IPC分类号： C07K14/08 ,  C07K14/095 ,  C07K14/415 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/24 ,  C12N9/72 ,  C12N9/78 ,  C12N9/84 ,  C12N15/113 ,  C12N15/12 ,  C12N15/33 ,  C12N15/40 ,  C12N15/82 ,  C12N15/83 ,  C12N15/86 ,  C12P41/00 ,  A01H5/00

CPC分类号： C12Y114/18001 ,  C07K14/005 ,  C07K14/415 ,  C12N15/1137 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8249 ,  C12N15/825 ,  C12N15/8289 ,  C12N15/86 ,  C12N9/00 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1074 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/6459 ,  C12N9/78 ,  C12N9/84 ,  C12P41/003 ,  C12Y302/01031 ,  C12Y304/21069 ,  C12N2710/22043 ,  C12N2710/22062 ,  C12N2750/12043 ,  C12N2770/00022 ,  C12N2770/00043 ,  C12N2770/00051 ,  C12N2770/32643 ,  C12N2770/32662 ,  C12N2770/32722 ,  C12N2770/32743 ,  C12N2770/32762 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2810/609

摘要： The present invention is directed to recombinant plant viral nucleic acids and to hosts infected thereby. The recombinant plant viral nucleic acids comprise a native plant viral subgenomic promoter, at least one non-native plant viral subgenomic promoter, a plant viral coat protein coding sequence, and optionally, at least one non-native nucleic acid sequence to be transcribed or expressed in the infected host plant. The recombinant plant viral nucleic acids are stable, capable of systemic infection and capable of stable transcription or expression in the plant host of the non-native nucleic acid sequences.

公开(公告)号：US11932861B2

公开(公告)日：2024-03-19

申请号：US17274559

申请日：2019-09-10

申请人： INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY

发明人： Jae Yean Kim ,  Tien Van Vu ,  Jihae Kim ,  Se Jeong Jeong ,  Hyun Jeong Kim ,  Seo-Jin Park ,  Mil Thi Tran ,  Velu Sivankalyani ,  Yeon Woo Sung ,  Thi Hai Duong Doan ,  Dibyajyoti Pramanik ,  Mahadev Rahul Shelake ,  Geon Hui Son

IPC分类号： C12N15/82

CPC分类号： C12N15/8203 ,  C12N2750/12043

摘要： A recombinant vector according to an embodiment is for genome editing without inserting a replicon into the plant genome in a T0 generation plant. The recombinant vector includes a geminivirus-based replicon between the sequence of LB (left border) and sequence of RB (right border) of Ti plasmid. A method of genome editing without inserting a replicon into the plant genome in a T0 generation plant according to an embodiment includes transforming a plant cell by inserting a foreign gene to the aforementioned recombinant vector.

公开(公告)号：US20140127749A1

公开(公告)日：2014-05-08

申请号：US14113201

申请日：2012-04-23

申请人： Hugh S. Mason ,  Fan Hong ,  George Bjorklund

发明人： Hugh S. Mason ,  Fan Hong ,  George Bjorklund

IPC分类号： C07K14/005

CPC分类号： C07K14/005 ,  C07K16/10 ,  C07K16/109 ,  C07K2317/30 ,  C12N15/8258 ,  C12N2750/12043 ,  C12N2760/14122 ,  C12N2760/14151 ,  C12N2760/16122 ,  C12N2760/16151 ,  C12N2800/22

摘要： The invention provides methods for making a target protein in a plant cell, and compositions thereof, wherein the target protein is a recombinant viral glycoprotein.

摘要翻译： 本发明提供了在植物细胞中制备靶蛋白的方法及其组合物，其中靶蛋白是重组病毒糖蛋白。

公开(公告)号：US20050204422A1

公开(公告)日：2005-09-15

申请号：US11129170

申请日：2005-05-13

申请人： Monto Kumagai ,  Guy Della-Cioppa ,  Jonathan Donson ,  Damon Harvey ,  Laurence Grill

发明人： Monto Kumagai ,  Guy Della-Cioppa ,  Jonathan Donson ,  Damon Harvey ,  Laurence Grill

IPC分类号： A01H5/00 ,  C07K14/08 ,  C07K14/095 ,  C07K14/415 ,  C07K14/705 ,  C12N1/19 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/24 ,  C12N9/72 ,  C12N9/78 ,  C12N9/84 ,  C12N15/09 ,  C12N15/113 ,  C12N15/12 ,  C12N15/40 ,  C12N15/53 ,  C12N15/82 ,  C12N15/83 ,  C12N15/86 ,  C12P21/04 ,  C12P41/00 ,  A01H1/00

CPC分类号： C12N15/1137 ,  C07K14/005 ,  C07K14/415 ,  C07K14/70571 ,  C12N9/00 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1074 ,  C12N9/1085 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/6459 ,  C12N9/78 ,  C12N9/84 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8249 ,  C12N15/825 ,  C12N15/8289 ,  C12N15/86 ,  C12N2710/22043 ,  C12N2710/22062 ,  C12N2750/12043 ,  C12N2770/00022 ,  C12N2770/00043 ,  C12N2770/00051 ,  C12N2770/32643 ,  C12N2770/32662 ,  C12N2770/32722 ,  C12N2770/32743 ,  C12N2770/32762 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2799/021 ,  C12N2810/609 ,  C12P41/003 ,  C12Y114/18001 ,  C12Y302/01031 ,  C12Y304/21069 ,  G01N2333/4353

摘要： The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.

摘要翻译： 本发明提供了用于在真核细胞的细胞质中表达抑制性RNA的新型遗传构建体。 抑制性RNA可以是反义RNA或共抑制RNA，并且起减少目标细胞中目的基因表达的作用。 本发明的遗传结构能够在真核细胞的细胞质中复制并且包含与编码多核苷酸的功能性组合的启动子区。 遗传结构可以设计成在植物细胞，酵母细胞和哺乳动物细胞的细胞质中复制。 当感兴趣的真核细胞是植物细胞时，遗传构建优选衍生自植物RNA病毒。 植物RNA病毒衍生的遗传结构可以使用植物病毒亚基因组启动子，包括与RNA编码区域功能组合的来自烟草花叶病毒的亚基因组启动子。 在本发明的一个优选实施方案中，诱导植物细胞产生升高水平的类胡萝卜素八氢番茄红素。 通过使用本发明的载体抑制酶八氢番茄红素去饱和酶的表达来实现提高的八氢番茄聚糖水平。

公开(公告)号：US5922602A

公开(公告)日：1999-07-13

申请号：US260546

申请日：1994-06-16

申请人： Monto Hiroshi Kumagai ,  Guy Richard della-Cioppa ,  Jonathan Donson ,  Damon Alan Harvey ,  Laurence K. Grill

发明人： Monto Hiroshi Kumagai ,  Guy Richard della-Cioppa ,  Jonathan Donson ,  Damon Alan Harvey ,  Laurence K. Grill

IPC分类号： A01H5/00 ,  C07K14/08 ,  C07K14/095 ,  C07K14/415 ,  C07K14/705 ,  C12N1/19 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/24 ,  C12N9/72 ,  C12N9/78 ,  C12N9/84 ,  C12N15/09 ,  C12N15/113 ,  C12N15/12 ,  C12N15/40 ,  C12N15/53 ,  C12N15/82 ,  C12N15/83 ,  C12N15/86 ,  C12P21/04 ,  C12P41/00 ,  C12N15/63

CPC分类号： C12N15/1137 ,  C07K14/005 ,  C07K14/415 ,  C07K14/70571 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8249 ,  C12N15/825 ,  C12N15/8289 ,  C12N15/86 ,  C12N9/00 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1074 ,  C12N9/1085 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N9/6459 ,  C12N9/78 ,  C12N9/84 ,  C12P41/003 ,  C12Y114/18001 ,  C12Y302/01031 ,  C12Y304/21069 ,  C12N2710/22043 ,  C12N2710/22062 ,  C12N2750/12043 ,  C12N2770/00022 ,  C12N2770/00043 ,  C12N2770/00051 ,  C12N2770/32643 ,  C12N2770/32662 ,  C12N2770/32722 ,  C12N2770/32743 ,  C12N2770/32762 ,  C12N2770/36143 ,  C12N2770/36162 ,  C12N2799/021 ,  C12N2810/609 ,  G01N2333/4353

摘要： The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.

公开(公告)号：US20240352435A1

公开(公告)日：2024-10-24

申请号：US18684481

申请日：2022-08-19

申请人： GRA&GREEN INC.

发明人： Masaki NIWA ,  Takehito KOBAYASHI ,  Yu SAWAI ,  Atsushi OKUDA

IPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/82

CPC分类号： C12N9/22 ,  C12N15/111 ,  C12N15/8223 ,  C12N2310/20 ,  C12N2750/12043

摘要： An object is to provide a plant genome editing technique with higher genome editing efficiency. This object is achieved by a polynucleotide comprising a site-specific nuclease expression cassette and a sequence that binds to a nuclear translocation factor and/or an intranuclear structure, the site-specific nuclease expression cassette comprising (b) a site-specific nuclease coding sequence and (c) a 3′UTR comprising a terminator.

公开(公告)号：US20190194680A1

公开(公告)日：2019-06-27

申请号：US16171158

申请日：2018-10-25

申请人： Arizona Board of Regents on Behalf of Arizona Stat

发明人： Hugh Mason ,  Charles Arntzen ,  Sun Hee Rosenthal ,  Sean Winkle ,  Andrew Diamos

IPC分类号： C12N15/82 ,  C07K16/28 ,  C12N7/00

CPC分类号： C12N15/8258 ,  C07K16/2887 ,  C07K2317/13 ,  C07K2317/14 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8218 ,  C12N2750/12043

摘要： A single vector or multiple separate vectors that contain two or more non-competing replicons for transient expression of the heavy and light chains of Rituximab in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures to optimize the expression is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of the multi-replicon single vector system for producing Rituximab in plant cells.

公开(公告)号：US20150368660A1

公开(公告)日：2015-12-24

申请号：US14762128

申请日：2014-01-22

申请人： Hugh MASON ,  Charles ARNTZEN ,  Sun Hee ROSENTHAL ,  Sean WINKLE ,  Andy DIAMOS ,  THE ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Hugh Mason ,  Charles Arntzen ,  Sun Hee Rosenthal ,  Sean Winkle ,  Andy Diamos

IPC分类号： C12N15/82 ,  C12N7/00 ,  C07K16/28

CPC分类号： C12N15/8258 ,  C07K16/2887 ,  C07K2317/13 ,  C07K2317/14 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8218 ,  C12N2750/12043

摘要： A single vector or multiple separate vectors that contain two or more non-competing replicons for transient expression of the heavy and light chains of Rituximab in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures to optimize the expression is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of the multi-replicon single vector system for producing Rituximab in plant cells.

摘要翻译： 描述了含有两个或更多个非竞争性复制子的单个载体或多个单独的载体，用于在烟草本生叶中短暂表达利妥昔单抗的重链和轻链。 还描述了将这些亚基蛋白正确组装到功能性低聚结构中以优化表达。 该系统通过消除对非竞争病毒的需要，推进植物瞬时表达技术，从而增强了用于在植物细胞中生产利妥昔单抗的多复制子单载体系统的实际商业应用。