公开(公告)号：US11110454B2

公开(公告)日：2021-09-07

申请号：US16115379

申请日：2018-08-28

申请人： NEXUS DX, INC.

发明人： Beom Seok Lee

IPC分类号： B01L3/00

摘要： A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers.

公开(公告)号：US11016106B2

公开(公告)日：2021-05-25

申请号：US15181283

申请日：2016-06-13

申请人： Nexus DX, Inc.

发明人： Michelle Davey ,  George Jackowski ,  Peter Kupchak ,  Eric Stanton

IPC分类号： G01N33/74 ,  C07K16/26 ,  G01N33/535 ,  C12N5/16 ,  G01N33/543 ,  G01N33/68 ,  G01N33/545

摘要： A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.

公开(公告)号：US11857963B2

公开(公告)日：2024-01-02

申请号：US17091692

申请日：2020-11-06

申请人： NEXUS DX, INC.

发明人： Beom Seok Lee

IPC分类号： B01L3/00

CPC分类号： B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/087 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/086 ,  Y10T436/2575

摘要： A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers.

公开(公告)号：US20180143108A1

公开(公告)日：2018-05-24

申请号：US15816449

申请日：2017-11-17

申请人： Nexus Dx, Inc.

发明人： Randall D. Madsen ,  Robert Klepper

IPC分类号： G01N1/28 ,  G01N33/53

CPC分类号： G01N1/28 ,  B01L3/5023 ,  B01L3/5082 ,  B01L3/527 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/046 ,  B01L2300/0609 ,  G01N33/5302

摘要： The present invention provides an apparatus for retaining solid state reagents and/or for processing sample for a diagnostic test, where the apparatus avoids liquid “hang-up” that would otherwise result in loss of sample or fluid volume during sample transfer. The invention further provides diagnostic methods that employ the apparatus, so as to provide sensitive and accurate analyte detection.

公开(公告)号：US09222875B2

公开(公告)日：2015-12-29

申请号：US14133199

申请日：2013-12-18

申请人： NEXUS DX, INC.

发明人： Richard Egan ,  Graham Lidgard ,  David Booker

IPC分类号： G01N21/01 ,  G01N21/55 ,  G01N21/84 ,  G01N33/487 ,  G06F19/00

CPC分类号： G01N21/01 ,  G01N21/84 ,  G01N21/8483 ,  G01N33/487 ,  G01N2201/0446 ,  G01N2201/126 ,  G16H10/40 ,  Y02A90/26

摘要： A diagnostic assay system including a test device and a scanning device are described. In one implementation, the scanning device includes a source of electromagnetic radiation, an optics assembly, a detector, and a microprocessor disposed within a chassis. The test device and scanning device may be configured to be movable relative to each other during operation of the scanning device.

摘要翻译： 描述了包括测试装置和扫描装置的诊断测定系统。 在一个实施方式中，扫描装置包括电磁辐射源，光学组件，检测器和布置在底盘内的微处理器。 测试装置和扫描装置可以被配置为在扫描装置的操作期间可相对于彼此移动。

公开(公告)号：US09823172B2

公开(公告)日：2017-11-21

申请号：US14164603

申请日：2014-01-27

申请人： Nexus DX, Inc.

发明人： Randall Madsen ,  Robert Klepper

IPC分类号： B01L3/00 ,  G01N1/28 ,  G01N33/53

CPC分类号： G01N1/28 ,  B01L3/5023 ,  B01L3/5082 ,  B01L3/527 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/046 ,  B01L2300/0609 ,  G01N33/5302

摘要： The present invention provides an apparatus for retaining solid state reagents and/or for processing sample for a diagnostic test, where the apparatus avoids liquid “hang-up” that would otherwise result in loss of sample or fluid volume during sample transfer. The invention further provides diagnostic methods that employ the apparatus, so as to provide sensitive and accurate analyte detection.

公开(公告)号：US20160011183A1

公开(公告)日：2016-01-14

申请号：US14739959

申请日：2015-06-15

申请人： Nexus Dx, Inc.

发明人： Richard Laswell Egan ,  Graham Peter Lidgard ,  David Dickson Booker ,  Christopher Johann Johnson ,  Alexander Belenky ,  Stan Vukajlovich

IPC分类号： G01N33/543

CPC分类号： G01N33/54306 ,  B01L3/0272 ,  B01L3/5023 ,  B01L3/5029 ,  G01N33/54366 ,  G01N2333/11

摘要： The invention provides compositions, systems and methods for detecting multiple analytes from a sample.

摘要翻译： 本发明提供了用于从样品中检测多种分析物的组合物，系统和方法。

公开(公告)号：US20140370617A1

公开(公告)日：2014-12-18

申请号：US14164603

申请日：2014-01-27

申请人： Nexus DX, Inc.

发明人： Randall Madsen ,  Robert Klepper

IPC分类号： G01N1/28 ,  B01L3/00

CPC分类号： G01N1/28 ,  B01L3/5023 ,  B01L3/5082 ,  B01L3/527 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/046 ,  B01L2300/0609 ,  G01N33/5302

摘要： The present invention provides an apparatus for retaining solid state reagents and/or for processing sample for a diagnostic test, where the apparatus avoids liquid “hang-up” that would otherwise result in loss of sample or fluid volume during sample transfer. The invention further provides diagnostic methods that employ the apparatus, so as to provide sensitive and accurate analyte detection.

摘要翻译： 本发明提供了一种用于保持固态试剂和/或用于处理用于诊断测试的样品的装置，其中装置避免了在样品转移期间会导致样品损失或液体体积的液体“挂起”。 本发明还提供了使用该装置的诊断方法，以便提供灵敏和准确的分析物检测。

公开(公告)号：US20140343860A1

公开(公告)日：2014-11-20

申请号：US14133199

申请日：2013-12-18

申请人： NEXUS DX, INC.

发明人： Richard EGAN ,  Graham LIDGARD ,  David BOOKER

IPC分类号： G01N21/01 ,  G01N21/84 ,  G01N33/487

CPC分类号： G01N21/01 ,  G01N21/84 ,  G01N21/8483 ,  G01N33/487 ,  G01N2201/0446 ,  G01N2201/126 ,  G16H10/40 ,  Y02A90/26

摘要： A diagnostic assay system including a test device and a scanning device are described. In one implementation, the scanning device includes a source of electromagnetic radiation, an optics assembly, a detector, and a microprocessor disposed within a chassis. The test device and scanning device may be configured to be movable relative to each other during operation of the scanning device.

摘要翻译： 描述了包括测试装置和扫描装置的诊断测定系统。 在一个实施方式中，扫描装置包括电磁辐射源，光学组件，检测器和布置在底盘内的微处理器。 测试装置和扫描装置可以被配置为在扫描装置的操作期间可相对于彼此移动。

公开(公告)号：US20220099687A1

公开(公告)日：2022-03-31

申请号：US17324685

申请日：2021-05-19

申请人： NEXUS DX, INC.

发明人： Michelle Davey ,  George Jackowski ,  Peter Kupchak ,  Eric Stanton

IPC分类号： G01N33/74 ,  G01N33/535 ,  C12N5/16 ,  G01N33/543 ,  C07K16/26 ,  G01N33/68

摘要： A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.